检索结果-南通市图书馆

Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration

引用

Neural Regeneration Research 2018年第1期13卷 100-104页

作者： marcela Fernandes Sandra Gomes Valente Rodrigo Guerra Sabongi Joao Baptista Gomes dos Santos Vilnei Mattioli Leite Henning Ulrich Arthur Andrade Nery Maria José da Silva Fernandes Division of Hand and Upper Limb Surgery Department of Orthopedics and Traumatology Universidade Federal de Sao Paulo Sao Paulo Brazil Department of Biochemistry Institute of Chemistry Universidade de Sao Paulo Sao Paulo Brazil Division of Neurosciences Department of Neurology and Neurosurgery Universidade Federal de Sao Paulo Sao Paulo Brazil

Studies have confirmed that bone marrow-derived mesenchymal stem cells （MSCs） can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs （BMSCs） is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs （ADSCs） have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups： sham （only sciatic nerve exposed）, Matrigel （MG; sciatic nerve injury ＋ intravenous transplantation of MG vehicle）, ADSCs （sciatic nerve injury ＋ intravenous MG containing ADSCs）, and BMSCs （sciatic nerve injury ＋ intravenous MG containing BMSCs） groups. Sciatic functional index was calculated to evaluate the function of injured sciatic nerve. Morphologic characteristics of nerves distal to the lesion were observed by toluidine blue staining. Spinal motor neurons labeled with Fluoro-Gold were quantitatively assessed. Compared with sham-operated rats, sciatic functional index was lower, the density of small-diameter fibers was significantly increased, and the number of motor neurons significantly decreased in rats with sciatic nerve injury. Neither ADSCs nor BMSCs significantly improved the sciatic nerve function of rats with sciatic nerve injury,increased fiber density, fiber diameters, axonal diameters, myelin sheath thickness, and G ratios （axonal diameter/fiber diameter ratios） in the sciatic nerve distal to the lesion site. There was no significant difference in the number of spinal motor neurons among ADSCs, BMSCs and MG groups. These results suggest that neither BMSCs nor ADSCs provide satisfactory results for peripheral nerve repair when using MG as the conductor for engraftment.

关键词： nerve regeneration mesenchymal stem cells adipose-derived mesenchmal stem cells sciatic nerve Matrigel sciatic functional index neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

哥伦比亚波哥大地区健康定制食品商业计划研究

哥伦比亚波哥大地区健康定制食品商业计划研究

引用

作者： Gina marcela Rodriguez Ramirez 上海外国语大学

学位级别：硕士

本研究的目的是确定一个框架,以评价建立一家提供饮食计划和特定营养建议服务公司的可行性。研究以创业理论、创业管理、物流管理等管理学理论为基础,因为这些理论对于食品行业成功的关键因素。本研究分析了两类数据,一是来自考察、个... 详细信息

本研究的目的是确定一个框架,以评价建立一家提供饮食计划和特定营养建议服务公司的可行性。研究以创业理论、创业管理、物流管理等管理学理论为基础,因为这些理论对于食品行业成功的关键因素。本研究分析了两类数据,一是来自考察、个人惊艳、访谈以及波哥大生活人群个人信息的一手数据,二是来自政府统计数据、报纸杂志、无政府组织及各类书籍的二手数据。研究发现,计划中的公司可以通过提供饮食计划和营养建议赢得潜在市场。通过认真控制公司结构,采用创新性的营销方案,该公司可以取得商业成功。研究发现,在当前形势下,新建公司必须建立强有力的商业模式,为公司内各环节提供支持,坚持为终端客户提供价值,确保公司可以获得持续利润。本研究的创新性在于为市场提供一种全新的产品组合,对在各类市场中计划进行类似创业活动者有一定参考作用。

关键词：创业物流创业管理食品工业

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

Health Risks due to Consumption of <i>Malus domestica</i>Golden Delicious Containing Heavy Metals

引用

Journal of Environmental Protection 2019年第4期10卷 577-594页

作者： Reyna del Carmen Lara-Severino Julia G. Cerón Breton Rosa M. Cerón Breton marcela Rangel Marrón Joaquín José parra Pérez Octavio Manuel Nah Cabrera Elidesmeralda Abreu Félix Sciences of Health Faculty Autonomous University of Carmen Ciudad del Carmen Campeche Mexico Chemistry Faculty Autonomous University of Carmen Ciudad del Carmen Campeche Mexico

The intake of heavy metals through food consumption is a food safety problem that severely impacts the health of consumers, so information about food intake and heavy metal concentrations in food products is required to estimate the potential risk to the health of the population. The main purpose of this study was to estimate the potential risks to human health from exposure to Cd, Cu, Ni, Pb and Zn through the intake of Malus domestica Golden Delicious consumed in the Mexican southeast, considering the age group and gender. The concentration of each of the metals was quantified by spectrometry of atomic absorption (AAE) and the daily intake of metals (DIM) and health risk indices through the consumption of apple: health risk index (HRI), total health risk index (THRI), target hazard coefficient (THQ) and the probability of carcinogenic risk (CR) were estimated. The concentrations of the metals analyzed in the apple samples showed the following decreasing order: Zn ≥ Ni ≥ Cu ≥ Pb ≥ Cd. The DIM values showed the following decreasing order: child > young adult > adoescent > elders. The health risk assessments (HRI, THRI, THQ and CR) obtained from the samples of Golden Delicious apples are located within the acceptable and/or tolerable limits, however, the increase in the intake of heavy metals through consumption of apples from establishments in the study area or the consumption of various foods contaminated with heavy metals may increase the health risk potential of consumers. For this reason, it is recommended to carry out studies on the concentration of heavy metals in food products, mainly those of greater popular consumption, to guarantee food security of the population.

关键词： Apple Heavy Metal Intake Health Risk Assessment Daily Intake and Carcinogenic Risk

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国文化软实力和中国梦的实现——以圣托马斯大学孔子学院为案例的研究，2008年至2017年

中国文化软实力和中国梦的实现——以圣托马斯大学孔子学院为案例...

引用

作者： marcela Fernanda Solis Walker 厦门大学

学位级别：硕士

随着中国经济在拉丁美洲的影响不断增强,中国政府也力图与拉丁美洲公民建立良好的关系。圣托马斯大学孔子学院的实证案例研究展示了中国如何利用语言和文化的传播推广,提升软实力并改善在包含智利在内等拉丁美洲国家的形象。本文通过软... 详细信息

随着中国经济在拉丁美洲的影响不断增强,中国政府也力图与拉丁美洲公民建立良好的关系。圣托马斯大学孔子学院的实证案例研究展示了中国如何利用语言和文化的传播推广,提升软实力并改善在包含智利在内等拉丁美洲国家的形象。本文通过软实力和中国文化软实力的理论,分析圣托马斯大学孔子学院在中国梦实现道路上中所扮演的角色。

关键词：孔子学院中国文化软实力中国梦

来源：

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

The role of rhizobacteria in rice plants: Growth and mitigation of toxicity

引用

Journal of Integrative Agriculture 2018年第12期17卷 2636-2647页

作者： marcela C F Rêgo Aline F Cardoso Thayná da C Ferreira Marta C C de Filippi Telma F V Batista Rafael G Viana Gisele B da Silva Plant Protection Laboratory Institute of Agrarian Sciences Federal Rural University of Amazon Phytopathology Laboratory Brazilian Enterprise for Agricultural Research-Rice and Beans

Allelopathic compounds reduce the growth and productivity of upland rice plants, especially in consecutive plantations. The rhizobacteria Pseudomonas fluorescens BRM-32111 and Burkholderia pyrrocinia BRM-32113 have been recorded as growth promoters in rice. This study was developed to understand the effect of the application of rhizobacteria on upland rice plants in consecutive plantations. Experiments were conducted in a completely randomized design with four replications of four treatments: rice seed inoculated with P. fluorescens BRM-32111, rice seed inoculated with B. pyrrocinia BRM-32113(both sown on soil with rice residue), non-inoculated plants sown on soil with rice residue(control with residue(WR)), and non-inoculated plants on soil with no residue(NR). Roots and seedling growth were adversely affected by allelopathic compounds in control WR plants. Plants inoculated with rhizobacteria P. fluorescens BRM-32111 or B. pyrrocinia BRM-32113 induced an increase of 88% in biomass, 3% in the leaf area, 40% in length, 67% in root biomass, 21% in chlorophyll a, 53% in chlorophyll(a+b), 50% in rate of carbon assimilation(A), 227% in A/rubisco carboxylation efficiency(Ci) and 63% in water use efficiency(WUE) compared to control WR plants. These results indicate that rhizobacteria P. fluorescens BRM-32111 and B. pyrrocinia BRM-32113 increase the tolerance of rice plants to stress from allelochemicals. There are possible practical agricultural applications of these results for mitigating the effects of environmental allelochemistry on upland rice.

关键词： allelopathy B.pyrrocinia P.fluorescens rhizobacteria rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Large-scale permafrost degradation as a primary factor in Larix sibirica forest dieback in the Khentii massif,northern Mongolia

引用

Journal of Forestry Research 2020年第1期31卷 197-208页

作者： David Juricka Jitka Novotna Jakub Houska Jana Parilkova Jan Hladky Vaclav Pecina Hana Cihlarova marcela Burnog Jakub Elbl Zdena Rosicka Martin Brtnicky Jindrich Kynicky Department of Geology and Pedology Faculty of Forestry and Wood TechnologyMendel University in BrnoZemedelska 161300 BrnoCzech Republic Central European Institute of Technology Brno University of TechnologyPurkynova 656/12361200 BrnoCzech Republic Institute of Water Structures Faculty of Civil EngineeringBrno University of TechnologyZizkova 1760200 BrnoCzech Republic

The objective of this study is to investigate the potential causes of widespread Larix sibirica *** observed in the Khentii massif of northern *** ratio of deadwood to living trees in affected stands in the Goricho region,the southernmost study site situated close to the Gobi Desert,was as high as 3.6:*** fluctuations monitored over 2 years using electrical impedance spectrometry revealed that the Goricho study site had higher soil moisture levels than the two less affected sites Barun Bayan and Dzun *** soil moisture was recorded in an area characterized by highly skeletal soils,ones with more than 35%by volume of rock fragments,and comparatively shallow soil horizons,from valley to *** layer of permafrost influencing hydrogeological processes is much deeper in the Goricho region compared to the undisturbed study *** analysis confirmed a significant number of dead *** on sites with developed *** forest stands,however damaged,grow in this region on well-drained scree slopes or on rocky *** mass mortality observed for *** may be directly linked to accelerated permafrost thaw in the area bordered by the Tuul and the Terelj *** assumption is that *** root system necrosis occurred as a result of long-term waterlogging of developed soils with high spatial heterogeneity,normally able to absorb high quantities of *** areas unaffected were scree fields and rocky bastions characterized by adequate *** of our findings support the primary stages of large-scale permafrost thaw,i.e.,correlating increases in soil moisture with increasing permafrost active layer thickness.

关键词： Larix sibirica Mortality Permafrost thawing Waterlogging Mongolia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Unsuccessful tuberculosis treatment outcomes across Brazil's geographical landscape before and during the COVID-19 pandemic:are we truly advancing toward the sustainable development/end TB goal?

引用

Infectious Diseases of Poverty 2024年第1期13卷 105-106页

作者： Reginaldo Bazon Vaz Tavares Thais Zamboni Berra Yan Mathias Alves marcela Antunes Paschoal Popolin Antonio Carlos Vieira Ramos Ariela Fehr Tartaro Clara Ferreira de Souza Ricardo Alexandre Arcencic Department of Maternal-Infant and Public Health Nursing University of São Paulo at Ribeirão Preto College of Nursing (USP/RPCN) Ribeirão Preto São Paulo Brazil Federal University of Tocantins Palmas Campus (FUT) Palmas Brazil State University of Minas Gerais Passos Campus (SUMG) Passos Brazil

Background Tuberculosis is one of the most significant infectious diseases for global public *** reallocation of healthcare resources and the restrictions imposed by the COVID-19 pandemic have hindered access to TB diag-nosis and *** in unfavorable outcomes of the disease have been observed in *** objective of this study was to analyze the spatial distribution of unfavorable TB treatment outcomes in Brazil before and during the *** An ecological study with spatial analysis was conducted with all 5569 municipalities in *** reported cases of tuberculosis between January 2010 and December 2021,as well as reported cases of COVID-19 from Febru-ary 2020 to December 2021,were *** outcomes studied encompass loss to follow-up,drug-resistant tuber-culosis,and *** Getis Ord GI*technique was employed to assess spatial association,and the Kernel density estimator was used to identify areas with concentrated increases or decreases in *** Local Moran's/was used to examine the spatial association between outcomes and cOVID-19 *** study was approved by the Research Ethics Committee of Ribeirao Preto Nursing School,University of Sao *** There were 134,394 cases of loss to follow-up,10,270 cases of drug resistance,and 37,863 *** of high and low values were identified for all three outcomes,indicating significant changes in the spatial distribu-tion *** in concentrations were observed for lost to follow-up cases in the Southeast,while reductions occurred in the Northeast,South,and ***-resistant tuberculosis experienced an increase in the Southern and Southeastern regions and a decrease in the Northeast and ***-related deaths showed notable concentra-tions in the Midwest,Northeast,South,and *** was an increase in high occurrence clusters for deaths after 2020 and 2021 in the *** The pandemic has brought additional chal

关键词： Tuberculosis COVID-19 Spatial analysis Ecological studies

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

TLR2 and TLR4 polymorphisms influence m RNA and protein expression in colorectal cancer

引用

World Journal of Gastroenterology 2015年第25期21卷 7730-7741页

作者： marcela Alcantara Proenca Juliana Garcia de Oliveira Aline Cristina Targa Cadamuro Maysa Succi Joao Gomes Netinho Eny Maria Goloni-Bertolo érika Cristina Pavarino Ana Elizabete Silva Department of Biology UNESPSao Paulo State University USC-Sacred Heart University PróReitoria de Pesquisa e Pós Graduacao Department of Surgery School of MedicineFAMERPSao Josédo Rio Preto 15090-000SPBrazil UPGEM School of MedicineFAMERPSao Josédo Rio Preto 15090-000SPBrazil

AIM: To evaluate the effect of promoter region polymorphisms of toll-like receptor(TLR)2-196 to-174 del and TLR4-1607T/C(rs10759932) on m RNA and protein expression in tumor tissue and of TLR4+896A/G(rs4986790) on colorectal cancer(CRC) ***: The TLR2-196 to-174 del polymorphism was investigated using allele-specific polymerase chain reaction(PCR) and the TLR4-1607T/C and TLR4+896A/G by PCR-restriction fragment length p o l y m o r p h i s m( R F L P). W e g e n o t y p e d 4 3 4 D N A samples from 194 CRC patients and 240 healthy individuals. The m RNA relative quantification(RQ) was performed in 40 tumor tissue samples by quantitative PCR Taq Man assay, using specific probes for TLR2 and TLR4 genes, and ACTB and GAPDH reference geneswere used as endogenous controls. Protein expression was analyzed by immunohistochemistry with specific primary ***: No association was found for TLR4-1607T/C and TLR4+896A/G by three statistical models(logadditive, dominant and recessive). However, based on dominant and log-additive models, the polymorphic variant TLR2-196 to-174 del was associated with increased CRC risk [dominant: odds ratio(OR) = 1.72, 95%CI: 1.03-2.89; P = 0.038 and log-additive: OR =1.59, 95%CI: 1.02-2.48; P = 0.039]. TLR2 m RNA expression was increased in tumor tissue(RQ = 2.36) when compared to adjacent normal tissue(RQ = 1; P < 0.0001), whereas the TLR4 m RNA showed a basal expression(RQ = 0.74 vs RQ = 1, P = 0.452). Immunohistochemistry analysis of TLR2 and TLR4 protein expression was concordant with the findings of m RNA expression. In addition, the TLR2-196 to-174 del variant carriers showed m RNA relative expression 2.19 times higher than wild-genotype carriers. The TLR2 protein expression was also higher for the TLR2-196 to-174 del variant carriers [117 ± 10 arbitrary unit(a.u.) vs 95 ± 4 a.u., P = 0.03]. However, for the TLR4-1607T/C polymorphism no significant difference was found for both m RNA(P = 0.56) and protein expression(P

关键词： Toll-like receptor 2 Toll-like receptor 4 Colorectal cancer Protein expression Gene expression Genetic polymorphisms

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis

引用

World Journal of Gastroenterology 2018年第47期24卷 5351-5365页

作者： marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva Department of Biology UNESPUniv. Estadual PaulistaCampus of Sao Jose do Rio PretoSao Jose do Rio PretoSao Paulo 15054-000Brazil Department of Surgery and Orthopedics Faculty of MedicineUNESPUniv. Estadual PaulistaCampus of BotucatuBotucatuSao Paulo 18618-687Brazil Endoscopy Center of Rio Preto Sao Jose do Rio PretoSao Paulo 15015-700Brazil Molecular Oncology Research Center Barretos Cancer HospitalBarretosSao Paulo 14784-400Brazil Life and Health Sciences Research Institute University of MinhoCampus GualtarBraga 4710-057Portugal ICVS/3B’s-PT Government Associate Laboratory Campus GualtarBraga 4710-057Portugal Cancer Biology and Therapeutics Group UCD Conway InstituteUniversity College DublinDublin D04 V1W8Ireland

AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction *** Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high *** Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4.

关键词： Colorectal cancer Colorectal adenoma Fusobacterium nucleatum Inflammation Cytokines MicroRNAs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Human papilloma virus and esophageal carcinoma in a Latin-American region

引用

World Journal of Gastroenterology 2009年第25期15卷 3142-3147页

作者： Roberto Herrera-Goepfert marcela Lizano Suminori Akiba Adela Carrillo-García Mauricio Becker-D'Acosta Department of Pathology National Cancer Institute Unit of Biomedical Research in Cancer National Cancer Institute and Biomedical Research InstituteNational Autonomous University of Mexico Department of Epidemiology and Preventive Medicine Kagoshima University Graduate School of Medical and Dental Sciences

AIM:To investigate the presence of high-risk human papilloma virus (HPV) in esophageal squamous cell carcinomas (ESCCs) in a non-selected Mexican ***: Cases with a pathological diagnosis of squamous cell carcinoma of the esophagus were obtained from Department of Pathology files, at the National Cancer Institute in Mexico City during the period between 2000 and 2008. Slides from each case were reviewed and cases with sufficient neoplastic tissue were selected for molecular analysis. DNA was extracted from paraffin-embedded tissue samples for polymerase chain reaction analysis to detect HPV DNA sequences. Demographic and clinical data of each patient were retrieved from corresponding clinical ***: HPV was detected in 15 (25%) of ESCCs. HPV-16 was the most frequently observed genotype, followed by HPV-18; HPV-59 was also detected in one case. Unfortunately, HPV genotype could not be established in three cases due to lack of material for direct sequencing, although universal primers detected the presence of HPV generic sequences. No low-risk HPV genotypes were found nor was HPV-16/18 co-infection. HPV presence in ESCC was not significantly associated with gender, age, alcohol consumption, smoking, anatomic location, or histologic grade. All patients belonged to low and very low socioeconomic strata, and were diagnosed at advanced disease stage. Male patients were most commonly affected and the male:female ratio in HPV-positive ESCC increased two- fold in comparison with HPV-negative cases (6.5:1 vs 3.1:1).CONCLUSION: High prevalence of high-risk HPV in ESCC in Mexico does not support the hypothesis that HPV-associated ESCC is more common in areas with higher ESCC incidence rates.

关键词： Esophagus Squamous cell carcinoma virusHuman papilloma virus High-risk human papilloma virus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：