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How to fill the biodiversity data gap:Is it better to invest in fieldwork or curation?

Plant Diversity 2024年第1期46卷 39-48页

作者： Carlos A.Vargas Marius Bottin Tiina Sarkinen James E.Richardson marcela Celis Boris Villanueva Adriana Sanchez Programa de Biología Facultad de Ciencias NaturalesUniversidad del RosarioBogotaD.C.Colombia Subdireccion Científica Jardín Botanico de Bogota“Jose Celestino Mutis”BogotaD.C.Colombia Independent Researcher BogotaD.C.Colombia Tropical Diversity Section Royal Botanic Garden EdinburghUK School of Biological Earth and Environmental SciencesUniversity College CorkCorkIreland Departamento de Química y Biología Universidad del NorteKm.5 Vía Puerto ColombiaArea Metropolitana de Barranquilla 081007Colombia

Data gaps and biases are two important issues that affect the quality of biodiversity information and downstream *** how best to fill existing gaps and account for biases is necessary to improve our current information most *** current main approaches for obtaining and improving data include(1)curation of biological collections,and(2)***,the comparative effectiveness of these approaches in improving biodiversity data remains little *** used the Flora de Bogota project to study the magnitude of change in species richness,spatial coverage,and sample coverage of plant records based on curation versus *** process of curation resulted in a decrease in species richness(synonym and error removal),but it significantly increased the number of records per *** contributed to a slight increase in species richness,via accumulation of new ***,curation led to increases in spatial coverage,species observed by locality,the number of plant records by species,and localities by species compared to ***,curationwas more efficient in producing new information compared to fieldwork,mainly because of the large number of records available in *** recommend intensive curatorial work as the first step in increasing biodiversity data quality and quantity,to identify bias and gaps at the regional scale that can then be targeted with *** stepwise strategy would enable fieldwork to be planned more costeffectively given the limited resources for biodiversity exploration and characterization.

关键词： Colombia Flora de Bogota Sample coverage Species richness Tropical Andes

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Environmental changes affect picoplanktonic composition in Antarctic Peninsula ponds

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Advances in Polar Science 2024年第1期35卷 108-122页

作者： Micaela DÍAZ Leonardo LAGOMARSINO Gabriela MATALONI Marianela BELTRÁN marcela LIBERTELLI Paulina FERMANI Laboratorio de Ecología Acuática INTECH-UNSAM-CONICETChascomús 7130Argentina Facultad de Ciencias Naturales y de la Salud UNPSJBComodoro Rivadavia 9000Argentina Laboratorio de Biodiversidad Limnología y Biología de la Conservación3IA-UNSAM-CONICETBuenos Aires 1650Argentina Departamento de Biología de Predadores Tope Instituto Antártico ArgentinoBuenos Aires 1650Argentina Laboratorio de Microbiología Ambiental IBIOMAR/CESIMAR-CENPAT-CONICETPuerto Madryn 9120Argentina

Antarctic Peninsula is experiencing one of the largest global warming events *** water bodies generated by the melting of snow in summer are numerous,and they might act as sentinels of climate change due to their rapid response and ability to integrate catchment *** in climate can influence the structure of microbial communities which dominate these freshwaters ***,we characterize three ponds at Cierva Point(Antarctic Peninsula)by examining their physico-chemical and morphological characteristics and we explored how different factors modify the structure of the microbial *** studied the abundance and biomass of heterotrophic bacteria,picocyanobacteria and picoeukaryote algae during January and February of two consecutive summers(2017 and 2018).We found that ponds had different limnological characteristics,due to their location,geomorphological features and presence of the surrounding flora and ***-chemical parameters as well as microbial community differed between ponds,months and *** 2017,most ponds were oligo to mesotrophic *** larger accumulated rainfall(as a result of environmental changes on the Antarctic Peninsula)during 2018,particularly in February,causes nutrient runoff into water *** affects those ponds with the highest seabird circulation,such as gentoo penguin,increasing *** a result,picoplanktonic abundances were higher,and the community structure shifts to a largely heterotrophic bacteria dominated *** results suggest that these communities could act as sentinels to environmental changes,anticipating a future with mostly hypertrophic ponds.

关键词： microorganisms freshwater environments climate change Cierva Point

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Determinant variables on the isotopic values of particulate organic matter in a neotropical floodplain

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Journal of Oceanology and Limnology 2024年第4期42卷 1173-1185页

作者： Vinícius de ANDRADE URBANO Matheus MAXIMILIAN RATZ SCOARIZE Gustavo HENRIQUE ZAIA ALVES Driele DELANIRA-SANTOS marcela da SILVA CAETANO Evanilde BENEDITO Departamento de Biologia(DBI) Laboratório de Ecologia EnergéticaUniversidade Estadual de Maringá(UEM)Maringá87020-900Brasil Programa de Pós-graduação em Ecologia de Ambientes Aquáticos Continentais(PEA) Universidade Estadual de Maringá(UEM)Maringá87020-900Brasil Departamento de Biologia Geral Universidade Estadual de Ponta Grossa(UEPG)Campus UvaranasPonta Grossa 84030-900Brasil Australian Rivers Institute(ARI) Griffith UniversityNathan QLD 4111Australia

Particulate organic matter(POM)is an important energy source for aquatic consumers,understanding its origin and composition is essential for understanding the energetic dynamics of aquatic *** aim of this study was to analyze the relationship between POM and phytoplankton(isotopic values and chlorophyll concentration)and abiotic variables during dry and rainy *** was conducted in rivers and lagoons in the floodplain of the Upper Paraná*** found a greater difference inδ13 C values of POM between sampling points than between seasons,indicating that the composition of regional sources influences the composition of POM more than dry and rainy *** addition,the concentration of chlorophyll during the dry season was positively correlated withδ13 C values during that rainy ***,we found a relationship between factors limiting the growth of phytoplankton andδ13 C values of POM,such as phosphate ions,indicating that variables that regulate phytoplankton growth tend to influence the composition of POM in river ***,maintaining the variables that regulate the phytoplankton community is of fundamental importance for the composition of POM,an important energy source in aquatic environments.

关键词： freshwater wetland stable isotopes food web subtropical environment

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A prototype for on-site generation of chlorinated disinfectant for use in rural aqueducts

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Water Science and Engineering 2024年第1期17卷 33-40页

作者： Diana marcela Cuesta Parra Felipe Correa Mahecha Andrés Felipe Rubio Pinzon Davidcamilo Ramirez Bustos Leonel Alveyro Teran Llorente Miguel Fernando Jimenez Jimenez Chemical and Environmental Engineering Department Fundacion Universidad de AmericaBogota 117111Colombia Chemical Engineering Department Fundacion Universidad de AmericaBogota 117111Colombia Mecanical Engineering Department Fundacion Universidad de AmericaBogota 117111Colombia

Sodium hypochlorite has significant potential as a sanitation solution in hard-to-reach *** studies have investigated the optimal electrolysis parameters for its production with volumes greater than 1o *** study evaluated sodium hypochlorite production through electrolysis in a 22-L prototype and identified the optimal operating *** were performed using graphite electrodes with areas of 68.4 cm^(2) at the laboratory scale and 1865.0 cm^(2) at the prototype scale.A design for experiments with different operating times,chloride concentrations,and electric current intensities was *** optimal operating time,sodium chloride concentration,and current intensity at the laboratory scale were 120 min,150 g of chloride per liter,and 3 A,respectively,leading to the production of 5.02 g/L of the disinfectant with an energy efficiency of 12.21 mg of Cl_(2) per *** the prototype scale,the maximum sodium hypochlorite concentration of 3.99 g of chloride per liter was achieved with an operating time of 120 min,a sodium chloride concentration of 100 g of chloride per liter,and a current intensity of 70 A,reaching an energy efficiency of 42.56 mg of Cl_(2) per *** addition,this study evaluated the influences of the chloride concentration,current intensity,and operating time on the production of sodium hypochlorite at the two scales,and formulated the equations showing the trends of sodium hypochlorite production and energy efficiency in the electrochemical *** 22-L prototype model for production of this oxidizing substance is promising for disinfection of large volumes of water in areas that are difficult to access.

关键词： Disinfection Electrolysis Energy efficiency Optimization Sodium hypochlorite

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Unsuccessful tuberculosis treatment outcomes across Brazil's geographical landscape before and during the COVID-19 pandemic:are we truly advancing toward the sustainable development/end TB goal?

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Infectious Diseases of Poverty 2024年第1期13卷 105-106页

作者： Reginaldo Bazon Vaz Tavares Thais Zamboni Berra Yan Mathias Alves marcela Antunes Paschoal Popolin Antonio Carlos Vieira Ramos Ariela Fehr Tartaro Clara Ferreira de Souza Ricardo Alexandre Arcencic Department of Maternal-Infant and Public Health Nursing University of São Paulo at Ribeirão Preto College of Nursing (USP/RPCN) Ribeirão Preto São Paulo Brazil Federal University of Tocantins Palmas Campus (FUT) Palmas Brazil State University of Minas Gerais Passos Campus (SUMG) Passos Brazil

Background Tuberculosis is one of the most significant infectious diseases for global public *** reallocation of healthcare resources and the restrictions imposed by the COVID-19 pandemic have hindered access to TB diag-nosis and *** in unfavorable outcomes of the disease have been observed in *** objective of this study was to analyze the spatial distribution of unfavorable TB treatment outcomes in Brazil before and during the *** An ecological study with spatial analysis was conducted with all 5569 municipalities in *** reported cases of tuberculosis between January 2010 and December 2021,as well as reported cases of COVID-19 from Febru-ary 2020 to December 2021,were *** outcomes studied encompass loss to follow-up,drug-resistant tuber-culosis,and *** Getis Ord GI*technique was employed to assess spatial association,and the Kernel density estimator was used to identify areas with concentrated increases or decreases in *** Local Moran's/was used to examine the spatial association between outcomes and cOVID-19 *** study was approved by the Research Ethics Committee of Ribeirao Preto Nursing School,University of Sao *** There were 134,394 cases of loss to follow-up,10,270 cases of drug resistance,and 37,863 *** of high and low values were identified for all three outcomes,indicating significant changes in the spatial distribu-tion *** in concentrations were observed for lost to follow-up cases in the Southeast,while reductions occurred in the Northeast,South,and ***-resistant tuberculosis experienced an increase in the Southern and Southeastern regions and a decrease in the Northeast and ***-related deaths showed notable concentra-tions in the Midwest,Northeast,South,and *** was an increase in high occurrence clusters for deaths after 2020 and 2021 in the *** The pandemic has brought additional chal

关键词： Tuberculosis COVID-19 Spatial analysis Ecological studies

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A multi-platform analysis of human gingival crevicular fluid reveals ferroptosis as a relevant regulated cell death mechanism during the clinical progression of periodontitis

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International Journal of Oral Science 2024年第2期16卷 265-279页

作者： Alfredo Torres M.Angélica Michea Ákos Végvári Marion Arce Valentina Pérez marcela Alcota Alicia Morales Rolando Vernal Mauricio Budini Roman A.Zubarev Fermín E.González Laboratory of Experimental Immunology&Cancer Faculty of DentistryUniversity of ChileSantiagoChile Department of Conservative Dentistry Faculty of DentistryUniversity of ChileSantiagoChile Division of Chemistry I Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSweden Periodontal Biology Laboratory Faculty of DentistryUniversidad de ChileSantiagoChile Laboratory of Cellular and Molecular Pathology Institute for Research in Dental SciencesFaculty of DentistryUniversity of ChileSantiagoChile

Ferroptosis is implicated in the pathogenesis of numerous chronic-inflammatory diseases,yet its association with progressive periodontitis remains *** investigate the involvement and significance of ferroptosis in periodontitis progression,we assessed sixteen periodontitis-diagnosed *** progression was clinically monitored over twelve weeks via weekly clinical evaluations and gingival crevicular fluid(GCF)collection was performed for further *** metrics,proteomic data,in silico methods,and bioinformatics tools were combined to identify protein profiles linked to periodontitis progression and to explore their potential connection with *** western blot analyses validated key ***,a single-cell RNA sequencing(scRNA-seq)dataset(GSE164241)for gingival tissues was analyzed to elucidate cellular dynamics during periodontitis *** progression was identified as occurring at a faster rate than traditionally *** samples from progressing and non-progressing periodontal sites showed quantitative and qualitatively distinct proteomic *** addition,specific biological processes and molecular functions during progressive periodontitis were revealed and a set of hub proteins,including SNCA,CA1,HBB,SLC4A1,and ANK1 was strongly associated with the clinical progression status of ***,we found specific proteins-drivers or suppressors-associated with ferroptosis(SNCA,FTH1,HSPB1,CD44,and GCLC),revealing the co-occurrence of this specific type of regulated cell death during the clinical progression of ***,the integration of quantitative proteomic data with scRNA-seq analysis suggested the susceptibility of fibroblasts to *** analyses reveal proteins and processes linked to ferroptosis for the first time in periodontal patients,which offer new insights into the molecular mechanisms of progressive periodontal *** findings may le

关键词： finding progression faster

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Modelling the development of biological structures displaying longitudinal geometries in vitro:culturing pluripotent stem cells on plasma-treated,growth factor-coupled polycaprolactone fibres

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Engineered Regeneration 2024年第1期5卷 124-138页

作者： Badwi B.Boumelhem Stuart T.Fraser Syamak Farajikhah Rachel A.Shparberg Michael B.Morris marcela M.M.Bilek Anyu Zhang Behnam Akhavan Simon Fleming Maryanne Large School of Physics Faculty of ScienceUniversity of SydneyAustralia Sydney Nano Institute University of SydneyAustralia School of Chemical and Biomolecular Engineering University of SydneyAustralia School of Medical Sciences Faculty of Medicine and HealthUniversity of SydneyAustralia School of Biomedical Engineering Faculty of EngineeringUniversity of SydneyAustralia School of Engineering University of NewcastleCallaghanNSW 2308Australia Hunter Medical Research Institute(HMRI) Precision Medicine ProgramNew Lambton Heights NSW 2305Australia

Many biological structures such as nerves,blood and lymphatic vessels,and muscle fibres exhibit longitudinal ge-ometries with distinct cell types extending along both the length and width of internal linear *** these three-dimensional structures in vitro is challenging:the best-defined stem-cell differentiation systems are mono-layer cultures or organoids using pluripotent stem *** stem cells can differentiate into functionally mature cells depending on the signals received,holding great promise for regenerative ***,the integration of in vitro differentiated cell types into diseased tissue remains a *** scaffolds can bridge this gap if the appropriate signalling systems are incorporated into the ***,we have taken a biomimicry approach to generate longitudinal structures in *** this approach,mouse embryonic stem cells are directed to differentiate to specific cell types on the surface of polycaprolactone(PCL)fibres treated by plasma-immersion ion implantation and to which with lineage-specifying molecules have been covalently *** demonstrate the simplicity and utility of our method for efficiently generating high yields of the following cell types from these pluripotent stem cells:neurons,vascular endothelial cells,osteoclasts,adipocytes,and cells of the erythroid,myeloid,and lymphoid *** arranged plasma-treated scaffolds with differentiated cell types could ultimately serve as a means for the repair or treatment of diseased or damaged tissue.

关键词： Pluripotent stem cells Lineage commitment Growth factors Biomaterial engineering Plasma-immersion ion implantation Covalent biomolecule attachment Polycaprolactone

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Assessing the impact of concurrent high-fructose and highsaturated fat diets on pediatric metabolic syndrome:A review

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World Journal of Clinical Pediatrics 2024年第2期13卷 62-79页

作者： Manuel Alejandro Vargas-Vargas marcela González-Montoya Olin Torres-Isidro Claudia Isabel García-Berumen Omar Ortiz-Avila Elizabeth Calderón-Cortés Christian Cortés-Rojo Instituto de Investigaciones Químico–Biológicas Universidad Michoacana de San Nicolás de HidalgoMorelia 58030MichoacánMexico Facultad de Enfermería Universidad Michoacana de San Nicolás de HidalgoMorelia 58020MichoacánMexico

High-saturated fat(HF)or high-fructose(HFr)consumption in children predispose them to metabolic syndrome(MetS).In rodent models of MetS,diets containing individually HF or HFr lead to a variable degree of ***,simultaneous intake of HF plus HFr have synergistic effects,worsening MetS *** children,the effects of HF or HFr intake usually have been addressed ***,we have reviewed the outcomes of HF or HFr diets in children,and we compare them with the effects reported in *** humans,HFr intake causes increased lipogenesis,hypertriglyceridemia,obesity and insulin *** the other hand,HF diets promote low grade-inflammation,obesity,insulin *** the deleterious effects of simultaneous HF plus HFr intake on MetS development in rodents,there is little information about the combined effects of HF plus HFr intake in *** aim of this review is to warn about this issue,as individually addressing the effects produced by HF or HFr may underestimate the severity of the outcomes of Western diet intake in the pediatric *** consider that this is an alarming issue that needs to be assessed,as the simultaneous intake of HF plus HFr is common on fast food menus.

关键词： Fructose Saturated fat Metabolic syndrome Insulin resistance Type 2 diabetes Ultra processed foods Children Obesity Dyslipidemia Non-alcoholic fatty liver disease

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Current concepts in the immunohistochemical evaluation of liver tumors

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World Journal of Hepatology 2015年第10期7卷 1403-1411页

作者： Anne K Koehne de Gonzalez marcela a salomao Stephen M Lagana Department of Pathology and Cell Biology College of Physicians and SurgeonsColumbia University

Immunohistochemistry often plays an important role in the evaluation of liver tumors. Recent advances have established a classification system for hepatocellular adenomas(HCAs) based on morphology,molecular alterations,and immunohistochemistry. Specifically,loss of liver fatty acid binding protein is seen in HNF1α-inactivated HCA,staining with serum amyloid A isseen in inflammatory HCA,and diffuse staining with glutamine synthetase(GS) is seen in β-catenin activated HCA. A panel of immunohistochemical stains including glypican-3(GPC-3),heat shock protein 70,and GS are useful in distinguishing HCC from non-malignant dysplastic nodules. Immunohistochemistry is also useful to determine whether a liver tumor is of primary hepatocellular or metastatic origin. Recently described markers useful for this purpose include arginase-1,GPC-3,and bile salt export pump. These newer markers may offer superior utility when compared to traditional markers of hepatocellular differentiation such as alpha-fetoprotein,hepatocyte paraffin-1,polyclonal carcinoembryonic antigen,and CD10. This paper will review recent advances in the immunohistochemical evaluation of liver tumors.

关键词： Immunohistochemistry Hepatocellularadenoma Focal nodular hyperplasia Hepatocellularcarcinoma

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The computerized LASSI-BC Test versus the Standard LASSI-L Paper-and-Pencil Version in Community-Based-Samples

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Advances in Alzheimer's Disease 2024年第1期13卷 11-25页

作者： Rosie E. Curiel Cid Alexandra Ortega Ubbo Visser marcela Kitaigorodsky D. Diane Zheng Diana Hincapie Kirsten Horne Crenshaw Ashleigh Beaulieu Brooke Bosworth Liz Gallardo Emory Neer Sofia Ramirez Elizabeth A. Crocco Mike Georgiou Efrosyni Sfakianaki David A. Loewenstein Center for Cognitive Neuroscience and Aging and Department of Psychiatry and Behavioral Sciences Miller School of Medicine University of Miami Miami FL USA Department of Computer Sciences University of Miami Coral Gables FL USA Nova Southeastern University Fort Lauderdale FL USA Department of Radiology and Nuclear Medicine Miller School of Medicine University of Miami Miami FL USA

Proactive Semantic Interference (PSI) and failure to recover from PSI (frPSI), are novel constructs assessed by the LASSI-L. These measures are sensitive to cognitive changes in early Mild Cognitive Impairment (MCI) and preclinical AD determined by Aβ load using PET. The goal of this study was to compare a new computerized version of the LASSI-L (LASSI-Brief Computerized) to the standard paper-and-pencil version of the test. In this study, we examined 110 cognitively unimpaired (CU) older adults and 79 with amnestic MCI (aMCI) who were administered the paper-and-pencil form of the LASSI-L. Their performance was compared with 62 CU older adults and 52 aMCI participants examined using the LASSI-BC. After adjustment for covariates (degree of initial learning, sex, education, and language of evaluation) both the standard and computerized versions distinguished between aMCI and CU participants. The performance of CU and aMCI groups using either form was relatively commensurate. Importantly, an optimal combination of Cued B2 recall and Cued B1 intrusions on the LASSI-BC yielded an area under the ROC curve of .927, a sensitivity of 92.3% and specificity of 88.1%, relative to an area under the ROC curve of .815, a sensitivity of 72.5%, and a specificity of 79.1% obtained for the paper-and-pencil LASSI-L. Overall, the LASSI-BC was comparable, and in some ways, superior to the paper-and-pencil LASSI-L. Advantages of the LASSI-BC include a more standardized administration, suitability for remote assessment, and an automated scoring mechanism that can be verified by a built-in audio recording of responses.

关键词： Mild Cognitive Impairment Proactive Semantic Interference LASSI-L Computerized Cognitive Assessment

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