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Postnatal development of fetuses with a single umbilical artery:differences between malformed and non-malformed infants

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World Journal of Pediatrics 2015年第1期11卷 61-66页

作者： jose Vicente Arcos-Machancoses Purificación marín-Reina Eugenia Romaguera-Salort Yolanda García-Camuñas Antonio Pérez-Aytés Máximo Vento Department of Pediatrics University & Polytechnic Hospital La Fe Valencia Spain Division of Neonatology University & Polytechnic Hospital La Fe Valencia Spain Department of Obstetrics & Gynecology University & Polytechnic Hospital La Fe Valencia Spain Division of Dysmorphology & Reproductive Assessment University & Polytechnic Hospital La Fe Valencia Spain

Background:The presence of a single umbilical artery(SUA)is a fetal soft marker of congenital *** the most common related malformations,there are cardiological,nephrourological and digestive anomalies,most of which are considered to have a vascular *** is an association between increased incidence of intrauterine growth retardation and adverse perinatal indicators,but whether this association is due to related anomalies or isolated SUA(SUA)is ***:We reviewed 96 cases of iSUA and non-isolated SUA(niSUA),diagnosed in a period of two years in a referral hospital for high-risk *** on prenatal explorations,including fetal ultrasonography and karyotyping,were *** was diagnosed when no malformations were found prenatally or in postnatal ***:Sixty-six newborns(68.8%)had no other anomalies and 30(31.3%)presented with a variety of malformations including heart diseases,urophaties,digestive,nervous and musculoskeletal disorders,genetic abnormalities and complex *** and nephrourological abnormalities were found to be the most frequent association with a SUA(both in 23.8%of malformed SUA newborns).Intrauterine growth restriction was not higher in iSUA newborns than in a normal *** allowed optimal prenatal diagnosis in most ***:The prognosis of the fetus with a SUA is determined by the presence of other malformations observed by an expert *** no other findings are made,only a routine physical examination should be performed in newborns,but no other complementary examinations are required.

关键词： cardiovascular abnormalities congenital abnormalities fetal growth retardation prenatal ultrasonography single umbilical artery

Influence of in vitro capacitation time on structural and functional human sperm parameters

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Asian Journal of Andrology 2020年第5期22卷 447-453页

作者： Paula Saez-Espinosa Natalia Huerta-Retamal Laura Robles-Gomez Manuel Aviles Jon Aizpurua Irene Velasco Alejandro Romero mar.a jose Gomez-Torres Department of Biotechnology Faculty of ScienceUniversity of AlicanteAlicante 03080Spain FISABI0-University Hospital of San Juan de Alicante Service of Gynecology and ObstetricsSan Juan de Alicante 03550Spain Departm ent of Cell Biology and Histology Faculty of MedicineUniversity of Murcia and IMIB-ArrixacaMurcia 30100Spain IVF Spain Reproductive MedicineAlicante 03540Spain University Hospital of San Juan de Alicante Service of Gynecology and ObstetricsSan Juan de Alicante 03550Spain Human Fertility Cathedra University of AlicanteAlicante 03080Spain.

A cascade of dramatic physiological events is linked to the sperm acrosome reaction and binding to the oocyte's zona pellucida during human sperm ***,structural and functional sperm changes during capacitation currently remain poorly ***,we performed a multibiomarker approach based on the utilization of sperm concentration,motility,viability,morphology,acrosome reaction,tyrosine phosphorylation,DNA fragmentation,and lectin-binding sites to analyze the impact caused by swim-up selection times(uncapacitated,1 h capacitated,and 4 h capacitated)on sperm function and structure in normozoospermic *** found that a 4 h swim-up capacitation increased sperm quality,because a large number of cells with normal morphology and lower DNA fragmentation rates were ***,the long-term capacitation induced a higher percentage of cells with tyrosine phosphorylation of the principal piece as well as a redistribution of lectin-binding ***,the multivariate biomarkers analyzed showed a less variable distribution on spermatozoa recovered after 4 h capacitation than that with the shorter capacitation *** findings stress the importance of capacitation time as a relevant factor in sperm quality with potential biological reproductive implications both for basic research and in assisted reproduction techniques.

关键词： acrosome reaction capacitation DNA fragmentation glycocalyx lectin-binding sites membrane integrity protein phosphorylation sperm selection

Confirmatory factor analysis of VISA-P scale and measurement invariance across sexes in athletes with patellar tendinopathy

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Journal of Sport and Health Science 2017年第3期6卷 365-371页

作者： Sergio Hernandez-Sanchez Ferran Abat mar.a D.Hidalgo Antonio I.Cuesta-Vargas Victor Segarra jose M.Sanchez-Ibanez Antonia Gomez-Conesa Department of Pathology and Surgery Physiotherapy AreaMiguel Hernandez UniversityValencia 03550Spain Sports Orthopaedic Surgeon ReSport ClinicBarcelona 08017Spain Department of Basic Psychology and Methodology University of MurciaMurcia 30100Spain Department of Physiotherapy Faculty of Health SciencesUniversity of MalagaMalaga 29009Spain

Background:The Victorian Institute of Sport Assessment–Patella(VISA-P) scale is the most condition-specific patient-reported outcome measure used to assess symptom severity in athletes with patellar *** exploratory factor analyses have been conducted to evaluate the scale's dimensionality,with inconsistent results,and the factor structure of the scale remains *** aims of the present study were to determine the factorial structure of the VISA-P scale using confirmatory factor analysis(CFA) and test measurement invariance across ***:The study included a convenience sample of 249 Spanish athletes with patellar *** was performed to assess factorial *** 1-and 2-factor models were *** invariance across sexes was evaluated via multi-group CFA with several fit indices using EQS 6.1 ***:The internal consistency coefficient was *** CFA models were examined and the 1-factor model in which errors for Items 7 and8 were correlated showed acceptable fit in terms of comparative fit index(CFI) and goodness-of-fit index(GFI) statistics(CFI = 0.93;GFI = 0.94;standardized root mean square residual = 0.06;root mean square error of approximation = 0.10;90% confidence interval:0.08–0.13).This model was invariant across ***:The 1-factor model of the Spanish version of the VISA-P scale(VISA-P-Sp) in which errors for Items 7 and 8 were correlated demonstrated relative fit in *** obtained via VISA-P-Sp can be compared between men and women without sexes *** studies should examine the VISA-P scale and other single-score patient-reported outcome measures concurrently.

关键词： Jumper's knee Measurement invariance Patient-reported outcome measure Structural validity

Chronic model of tympanic perforation in rats with mitomycin C and dexamethasone

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Journal of Otology 2019年第1期14卷 12-16页

作者： Tomás Esteban Noemí M.Atucha jose mar.a Moraleda Joaquín Garcia-Esta? Carmen L.Insausti Javier Moraleda-Deleyto Servicio de Otorinolaringologia Hospital General Universitario Santa Lucia Departamento de Fisiologia Facultad de Medicina Universidad de Murcia Instituto Murciano de Investigacion Biosanitaria Departamento de Medicina Facultad de Medicina Universidad de MurciaInstituto Murciano de Investigacion Biosanitaria ENT Service University Hospital of Lewisham

A rat model of chronic tympanic membrane perforation was developed to be used in the search of new materials for the sealing of these perforations. A longitudinal study was carried out in rats subjected to incisional myr-ingotomy followed by the application of mitomycin C alone or with dexamethasone. Rats were checked at days 3, 7,1 0,1 4 and weekly thereafter until perforation closure, for up to 6 months. The addition of dexamethasone is a key component in order to obtain a chronic opening. Myringotomies treated w ith saline had a mean healing time of 8.5 days. At 8 weeks, between 62.5% and 77.7% of tympanic membranes treated w ith mitomycin C and dexamethasone remained perforated and at 6 months this number fell to 21.4%. This technique is able to maintain most tympanic membrane perforations patent for at least 8 weeks. This rat model is adequate for its use in preclinical or translational research.

关键词： Animal model Chronic tympanic membrane perforation Mitomycin C Myringotomy Dexamethasone

Biomagnetic techniques for evaluating gastric emptying,peristaltic contraction and transit time

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World Journal of Gastrointestinal Pathophysiology 2011年第5期2卷 65-71页

作者： jose mar.a De la Roca-Chiapas Teodoro Cordova-Fraga Division of Health Sciences Department of PsychologyUniversity of GuanajuatoCampus Leon Department of Physical Engineering University of GuanajuatoCampus Leon

Biomagnetic techniques were used to measure motility in various parts of the gastrointestinal(GI) tract,particularly a new technique for detecting magnetic markers and tracers.A coil was used to enhance the signal from a magnetic tracer in the GI tract and the signal was detected using a fluxgate magnetometer or a magnetoresistor in an unshielded *** of esophageal transit time were affected by the position of the *** reproducibility of estimates derived using the new biomagnetic technique was greater than 85% and it yielded estimates similar to those obtained using *** technique is suitable for studying the effect of emotional state on GI physiology and for measuring GI transit *** biomagnetic technique can be used to evaluate digesta transit time in the esophagus,stomach and colon,peristaltic frequency and gastric emptying and is easy to use in the hospital setting.

关键词： Biomagnetic techniques Magnetogastrography Gastric emptying Scintigraphy Peristaltic contractions

Correlation between Iron Reducibility in Natural and Iron-Modified Clays and Its Adsorptive Capability for Arsenic Removal

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Advances in Materials Physics and Chemistry 2016年第5期6卷 129-139页

作者： Irma Lia Botto Simonetta Tuti mar.a jose Gonzalez Delia Gazzoli CEQUINOR (CCT-La Plata-UNLP) La Plata Argentina Dipartimento di Scienze Universitá Roma Tre Roma Italy INREMI (CICPBA-UNLP) La Plata Argentina Dipartimento di Chimica Universitá La Sapienza Roma Italy

The study reports aspects that allowed to correlate structural and redox properties of iron species deposited on clay minerals with the capacity of geomaterials for arsenic removal. Natural ferruginous clays as well as an iron-poor clay chemically modified with Fe(III) salt (ferrihydrite species) were investigated as adsorbents of the arsenate(V) in water. The study, carried out from minerals from abundant Argentinean deposits, was conducted with the aid of different techniques such as X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM-EDS), Raman Spectroscopy, ICP-AES (Inductively Coupled Plasma) chemical analysis and Temperature Programmed Reduction (TPR). This last technique allowed to detect availability of iron species in oxidic environment with different structural complexity and to determine active sites, accessible for arsenate(V) adsorption. The effect was observed through temperature dependence of the first Fe(III) reduction step (below 570°C) of iron-oxide species. The sequence of reducibility: ferrihydrite > hydrous oxide (goethite) > anhydrous oxide (hematite) > structural iron in clay was in agreement with the availability of iron active sites for the reducing process as well as for the arsenate adsorption. The important role of very high iron content in original samples was also observed. The chemical activation of iron-poor clay by a simple and feasible modification with Fe(III) solutions promoted the deposition of the ferrihydrite active phase with an increase of 2.81% (expressed as Fe2O3) respect to the original content of 1.07%, constituting an accessible and eco-friendly technological alternative to solve the environmental problem of water containing arsenic.

关键词： Temperature Programmed Reduction Iron-Oxide Species Arsenic Removal

Presacral venous bleeding during mobilization in rectal cancer

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World Journal of Gastroenterology 2017年第9期23卷 1712-1719页

作者： jose Enrique Casal Núnez Vincenzo Vigorita Alejandro Ruano Poblador Ana mar.a Gay Fernández mar.a ángeles Toscano Novella Nieves Cáceres Alvarado Lucinda Pérez Dominguez Coloproctology Unit and General and Digestive Surgery Unit Vigo University Hospital Complex36212 VigoSpain

To analyze the anatomy of sacral venous plexus flow, the causes of injuries and the methods for controlling presacral hemorrhage during surgery for rectal *** review of the databases MEDLINE® and Embase™ was conducted, and relevant scientific articles published between January 1960 and June 2016 were examined. The anatomy of the sacrum and its venous plexus, as well as the factors that influence bleeding, the causes of this complication, and its surgical management were *** is a review of 58 published articles on presacral venous plexus injury during the mobilization of the rectum and on techniques used to treat presacral venous bleeding. Due to the lack of cases published in the literature, there is no consensus on which is the best technique to use if there is presacral bleeding during mobilization in surgery for rectal cancer. This review may provide a tool to help surgeons make decisions regarding how to resolve this serious *** series of alternative treatments are described; however, a conventional systematic review in which optimal treatment is identified could not be performed because few cases were analyzed in most publications.

关键词： Presacral hemorrhaging Rectal surgery Sacral venous plexus Pelvic surgery Sacral anatomy

Cadaveric bone marrow mesenchymal stem cells:first experience treating a patient with large severe burns

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Burns & Trauma 2015年第2期3卷 74-82页

作者： Eduardo Mansilla Gustavo H.marín Mirta Berges Silvia Scafatti Jaime Rivas Andrea Núñez martin Menvielle Roberto Lamonega Cecilia Gardiner Hugo Drago Flavio Sturla Mercedes Portas Silvia Bossi mar.a Victoria Castuma Sandra Peña Luengas Gustavo Roque Karina martire jose mar.a Tau Gabriel Orlandi Adrian Tarditti Tissue Engineering Regenerative Medicine and Cell Therapies LaboratoryCUCAIBAProvince of Buenos Aires Ministry of HealthTransplantation ProgramLa PlataProvince of Buenos AiresArgentina Burns and Plastic Surgery Department San Martín HospitalProvince of Buenos Aires Ministry of HealthLa PlataProvince of Buenos AiresArgentina Burns Hospital Buenos Aires CityArgentina Department of Chemistry Mayaguez CampusUniversity of Puerto RicoMayaguezPuerto RicoUSA.

Background:In January 2005,Rasulov et *** published"First experience in the use of bone marrow mesenchymal stem cells(MSCs)for the treatment of a patient with deep skin burns".Here,we present the first ever treated patient with cadaveric bone marrow mesenchymal stem cells(CMSCs)in the history of ***:A young man,who severely burned 60%of his total body surface with 30%of full-thickness burns while working with a grass trimmer that exploded,was involved in the *** were obtained from the bone marrow of a cadaver donor in a routine procurement procedure of CUCAIBA,the Province of Buenos Aires,Argentina,Ministry of Health,Transplantation Agency,cultured,expanded,and applied on the burned surfaces using a fibrin spray after early ***:So far,our preliminary experience and our early results have been very impressive showing an outstanding safety data as well as some impressive good results in the use of ***:Based on al this,we think that improvements in the use of stem cells for burns might be possible in the near future and a lot of time as well as many lives could be saved by many other research teams all over the *** will probably be a real scientific opportunity in Regenerative Medicine as well as in Transplantation.

关键词： Burns Cadaveric Transplantation Skin regeneration Mesenchymal stem cells

Determination of altitudes of the three main Ecuadorian summits through GNSS positioning

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Geodesy and Geodynamics 2022年第4期13卷 343-351页

作者： mar.a jose Merizalde Mora Cesar Alberto Leiva Gonzalez Dennys Alexander Enriquez Hidalgo Theofilos Toulkeridis Universidad de Las Fuerzas Armadas ESPE SangolquiEcuador Instituto Geografico Militar IGM QuitoEcuador Universidad de Especialidades Turisticas UDET QuitoEcuador

Determining the altitude of mountains is one of the most fundamental challenges for ***,the main objective of the study is to determine the altitudes of the three main summits in Ecuador through GNSS positioning,referring to the official vertical datum of Ecuador and the geo-potential surface(W_(o)).The three peaks are active volcanoes named Chimborazo,Cotopaxi and Cayambe,all of which are known to be over 5700 m above sea *** GNSS was performed on the summit of the volcanoes,and the GPS leveling method was applied on nearby *** these summits,the correction of the potential difference between the mean sea level and W_(o) was *** altitudes referred to the mean sea level were compared with other registered heights,and the results are more accurate than previous geodetic missions.

关键词： Altitude Volcanoes GNSS GPS leveling Geopotential