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Inhibition of Acinetobacter baumannii Biofilm Formation by Methanolic Extract of Nothoscordum bivalve

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Advances in Microbiology 2018年第5期8卷 422-438页

作者： Hernández-marín David Alejandro Munoz-Ortega martín Humberto Guevara-Lara Fidel Gúzman-Lucio marco Antonio Sánchez-García Eduardo Laboratorio de Química Analítica Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónSan Nicolás de los GarzaNuevo LeónMéxico Laboratorio de Patología Molecular Experimental Departamento de QuímicaCentro de Ciencias BásicasUniversidad Autónoma de AguascalientesAguascalientesMéxico Laboratorio de Biotecnología y Funcionalidad de Alimentos Departamento de QuímicaCentro de Ciencias BásicasUniversidad Autónoma de AguascalientesAguascalientesMéxico departamento de Botánica Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónMonterreyNuevo LeónMéxico

Acinetobacter baumannii is one of the most prevalent pathogens in nosocomial infections and has been cause of concern in recent years because, it has presented multiresistance to antibiotics and besides can form biofilms on biotic and abiotic surfaces like tissues and medical devices. Therefore, the search for new alternatives of natural origin to inhibit biofilm formation is being conducted. In the present investigation, sub-lethal concentrations (5.61 mg/mL, 3.74 mg/mL and 1.87 mg/mL) of methanolic extract of Nothoscordum bivalve were evaluated, showing biofilm formation inhibition up to 40.8%, in one nosocomial isolated of A. baumannii by the microtiter biofilm formation assay using crystal violet. On the other hand, the concentrations of 5.61 mg/mL and 3.74 mg/mL, caused an overexpression (up to 15.4 times) in the genes involved in the formation of biofilm (abaI, bap and csuE);due to this, the interaction of the extract with the bacteria was analyzed by scanning electron microscopy (SEM) and cellular damage was observed in the structure and stability of biofilm.

关键词： Biofilm Sub-Lethal Nothoscordum bivalve Acinetobacter baumannii

Reservoir Processes Related to Exploitation in los Azufres (México) Geothermal Field Indicated by Geochemical and Production Monitoring Data

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International Journal of Geosciences 2015年第9期6卷 1048-1059页

作者： Víctor Manuel Arellano Rosa mar.a Barragán Miguel Ramírez Siomara López Alfonso Aragón Adriana Paredes Emigdio Casimiro Lisette Reyes Instituto de Investigaciones Eléctricas Gerencia de Geotermia Cuernavaca México Comisión Federal de Electricidad Gerencia de Proyectos Geotermoeléctricos Morelia México Comisión Federal de Electricidad Residencia Los Azufres Campamento Agua Fría México

A combined analysis of geochemical and production data of 39 wells of the los Azufres (Mexico) geothermal field (227.4 MWe) over time was developed to investigate the exploitation-related processes for 2003-2011. In the south zone, important effects of reinjection were observed through Cl increases in some wells (up to 8000 mg/kg) while in wells with significant boiling, Cl has decreased. In most of the north zone wells, the variations in gas data indicated boiling and condensation of a highly gas-depleted brine, which seems to consist of reinjection fluids. It is suggested that this process maintains the production in the zone relatively stable. The main reservoir exploitation-related processes found were: 1) production of reinjection returns;for this, it was possible to distinguish a) wells that produce liquid and steam from injection, and b) wells that produce steam from injection and sometimes condensed steam from injection;2) boiling: two types of boiling were identified: a) boiling with steam gain, and b) boiling with steam loss. The results indicated that an effective reservoir recharge occurs since very moderate production declining rates were found.

关键词： los Azufres Geothermal Field Well Simulation Fluid Geochemistry Well Bottom Conditions Production Wells

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水表面波矩型谐振器非线性共振曲线的测量

物理学报 1985年第6期 796-800页

作者：吴君汝 A.LARRAzA I.ruDNICK 南京大学物理系 department of Physics University of California

本文是在作者最近发现的非传播型水表面孤波的基础上对有关机制的进一步探讨。实验表明水表面波矩型谐振器非线性共振曲线可向低频端或向高频端偏移,决定这种偏移方向的参数是平衡时液面的高度。作者并系统地测量了在不同液面高度下的... 详细信息

本文是在作者最近发现的非传播型水表面孤波的基础上对有关机制的进一步探讨。实验表明水表面波矩型谐振器非线性共振曲线可向低频端或向高频端偏移,决定这种偏移方向的参数是平衡时液面的高度。作者并系统地测量了在不同液面高度下的共振曲线,确定了共振曲线在非线性响应下不发生偏移的转换液面高度。

关键词：共振曲线谐振器共振器表面波波浪电容传感器矩型表面水波共振频率非线性共振滚动台液面高度

R&3D技术创新风险与失败案例研究——以洁净煤技术为例

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科学学与科学技术管理 2014年第9期35卷 102-110页

作者：张汉威苏竣汝鹏周源清华大学公共管理学院北京100084

基于R&3D的框架,拓展了Tassey的技术风险曲线,分别从理论和实证两方面进行验证。在理论方面,综述了技术创新风险的典型性研究,从融合的角度探讨了技术创新风险的类型和水平,对示范和推广阶段的风险进行了综合评定。在实践方面,以洁净煤... 详细信息

基于R&3D的框架,拓展了Tassey的技术风险曲线,分别从理论和实证两方面进行验证。在理论方面,综述了技术创新风险的典型性研究,从融合的角度探讨了技术创新风险的类型和水平,对示范和推广阶段的风险进行了综合评定。在实践方面,以洁净煤技术领域为例,选取了燃煤磁流体技术、增压流化床燃烧联合循环发电技术、多喷嘴对置式水煤浆气化技术作为典型案例进行成功与失败的对比研究。理论研究结果表明,示范阶段交织存在着多种类型、不同程度的风险,极易导致技术创新的失败。实证研究结果表明,(科学)技术就绪水平、市场和产业就绪水平、制度就绪水平是决定R&3D中前三个阶段成败的重要因素,应予以特别的关注和重视。

关键词：技术创新风险失败洁净煤

Arachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitis via AMPK and mTOR regulation

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World Journal of Gastroenterology 2020年第34期26卷 5101-5117页

作者： David Fernández-Ramos Fernando Lopitz-Otsoa Laura delacruz-Villar Jon Bilbao martina Pagano Laura Mosca Maider Bizkarguenaga marina Serrano-Macia Mikel Azkargorta mar.a Iruarrizaga-Lejarreta Jesús Sot Darya Tsvirkun Sebastiaan martijn van Liempd Felix M Goni Cristina Alonso mar.a Luz martínez-Chantar Felix Elortza Liat Hayardeny Shelly C Lu JoséM Mato Precision Medicine and Metabolism Laboratory Centro de Investigación Cooperativa en Biociencias(CIC bioGUNE)Derio 48160BizkaiaSpain CIBERehd-Centro de Investigación Biomédica en Red de enfermedades hepáticas y digestivas Madrid 28029Spain department of Precision Medicine University of Campania"Luigi Vanvitelli"Naples 80138Italy Liver Disease Laboratory Centro de Investigación Cooperativa en Biociencias(CIC bioGUNE)Derio 48160Spain Proteomics Platform Centro de Investigación Cooperativa en Biociencias(CIC bioGUNE)Derio 48160Spain OWL Metabolomics Derio 48160Spain Instituto Biofisika(UPV/EHU CSIC)Leioa 48940SpainDepartamento de Bioquímica y Biología MolecularUniversidad del País VascoLeioa 48940Spain Pre-clinical and Chemistry Manufacturing and ControlsGalmed PharmaceuticalsTel Aviv 6578317Israel Metabolomics Platform Centro de Investigación Cooperativa en Biociencias(CIC bioGUNE)Derio 48160Spain Division of Digestive and Liver Diseases Cedars-Sinai Medical CenterLos AngelesCA 90048United States

BACKGROUND Arachidyl amido cholanoic acid(Aramchol)is a potent downregulator of hepatic stearoyl-CoA desaturase 1(SCD1)protein expression that reduces liver triglycerides and fibrosis in animal models of *** a phase IIb clinical trial in patients with nonalcoholic steatohepatitis(NASH),52 wk of treatment with Aramchol reduced blood levels of glycated hemoglobin A1c,an indicator of glycemic *** To assess lipid and glucose metabolism in mouse hepatocytes and in a NASH mouse model[induced with a 0.1%methionine and choline deficient diet(0.1MCD)]after treatment with *** Isolated primary mouse hepatocytes were incubated with 20μmol/L Aramchol or vehicle for 48 ***,analyses were performed including Western blot,proteomics by mass spectrometry,and fluxomic analysis with 13C-uniformly labeled *** the in vivo part of the study,male C57BL/6J mice were randomly fed a control or 0.1MCD for 4 wk and received 1 or 5 mg/kg/d Aramchol or vehicle by intragastric gavage for the last 2 *** metabolomics were assessed using ultra-high-performance liquid chromatography-time of flight-MS for the determination of glucose metabolism-related *** Combination of proteomics and Western blot analyses showed increased AMPK activity while the activity of nutrient sensor mTORC1 was decreased by Aramchol in *** translated into changes in the content of their downstream targets including proteins involved in fatty acid(FA)synthesis and oxidation[PACCα/β(S79),SCD1,CPT1A/B,HADHA,and HADHB],oxidative phosphorylation(NDUFA9,NDUFB11,NDUFS1,NDUFV1,ETFDH,and UQCRC2),tricarboxylic acid(TCA)cycle(MDH2,SUCLA2,and SUCLG2),and ribosome(P-p70S6K[T389]and P-S6[S235/S236]).Flux experiments with 13Cuniformely labeled glucose showed that TCA cycle cataplerosis was reduced by Aramchol in hepatocytes,as indicated by the increase in the number of rounds that malate remained in the TCA ***,liver metabolomic analysis showed

关键词： Nonalcoholic fatty liver disease Steatohepatitis Methionine and choline deficient diet Tricarboxylic acid cycle Hemoglobin A1c Stearoyl-CoA desaturase 1

能谱CT单能量联合mar去除髋关节置换物伪影的临床研究

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局解手术学杂志 2021年第10期30卷 894-899页

作者：苏伟冯如无锡市第九人民医院放射科江苏无锡214062 无锡市人民医院医学影像科江苏无锡214023

目的探讨能谱CT单能量联合多伪影去除(mar)技术对去除髋关节置换物伪影的应用价值。方法收集于我院行髋关节置换术的31例患者的临床资料,患者均行能谱CT检查联合mar技术扫描,基于原始能谱CT扫描数据重建70~140 KeV以10个KeV能级为间隔的... 详细信息

目的探讨能谱CT单能量联合多伪影去除(mar)技术对去除髋关节置换物伪影的应用价值。方法收集于我院行髋关节置换术的31例患者的临床资料,患者均行能谱CT检查联合mar技术扫描,基于原始能谱CT扫描数据重建70~140 KeV以10个KeV能级为间隔的8组单能量图像(非mar组)及8组单能量mar图像(mar组)。由2名高年资影像医师对每组图像进行主观评分。检测相同能级下2组图像上髋关节假体周围金属高低密度伪影区、周围肌肉、周围骨组织、膀胱及皮下脂肪中的CT值及标准差(SD)值,计算并比较2组图像中假体周围肌肉、周围骨组织、膀胱中信噪比(SNR)和伪影指数(AI)。结果各能级中mar组图像主观评分均高于非mar组(Pmar组假体周围金属低密度区CT值均高于非mar组(Pmar组假体周围金属低密度区SD值均低于非mar组(Pmar组假体周围金属高密度区CT值均低于非mar组(Pmar组假体周围金属高密度区SD值均低于非mar组(Pmar组假体周围肌肉SNR值均高于非mar组(Pmar组(Pmar组假体周围骨组织SNR值均高于非mar组(Pmar组假体周围骨组织AI值均低于非mar组(Pmar组膀胱SNR值均高于非mar组(Pmar组(Pmar技术对去除髋关节置换物伪影具有较好效果,可提高图像清晰度,进而提高诊断准确度,具备良好临床应用价值。

关键词：能谱成像髋关节置换金属伪影体层摄影术

经腹腔镜和经阴道子宫肌瘤剔除术临床效果对比分析

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现代医药卫生 2014年第12期30卷 1783-1784页

作者：许茹娜雷州市人民医院广东雷州524200

目的探讨经腹腔镜子宫肌瘤剔除术(LM)与经阴道子宫肌瘤剔除术(TVM)2种手术方式的临床特点及应用价值。方法选取2011年1月至2013年5月在该院行子宫肌瘤剔除术患者108例,分为LM组55例和TVM组53例。LM组行经LM治疗,TVM组行TVM治疗,观察两... 详细信息

目的探讨经腹腔镜子宫肌瘤剔除术(LM)与经阴道子宫肌瘤剔除术(TVM)2种手术方式的临床特点及应用价值。方法选取2011年1月至2013年5月在该院行子宫肌瘤剔除术患者108例,分为LM组55例和TVM组53例。LM组行经LM治疗,TVM组行TVM治疗,观察两组患者手术时间、术中出血、术后疼痛、住院时间及术后并发症等指标。结果两组患者手术时间、术中出血量、肠功能恢复时间等比较,差异均有统计学意义(P0.05)。结论 2种手术均有优缺点和局限性,临床医生应根据患者的个体情况采用适合的手术方式,以获得最佳治疗效果。

关键词：腹腔镜检查平滑肌瘤外科学阴道外科学子宫肿瘤外科学治疗结果对比研究

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C语言与C++语言几点差异的应用实例分析

河北软件职业技术学院学报 2016年第1期18卷 52-54页

作者：周建儒四川信息职业技术学院

C语言是面向过程的编程语言,C++语言是面向对象的编程语言,虽然它们是两种不同的程序设计语言,但是在基础语法的实际应用中既有相似之处,又有细微差别,尤其是在字符串与字符数组的混合使用及互相转换过程中体现得更为明显,这些差异是其... 详细信息

C语言是面向过程的编程语言,C++语言是面向对象的编程语言,虽然它们是两种不同的程序设计语言,但是在基础语法的实际应用中既有相似之处,又有细微差别,尤其是在字符串与字符数组的混合使用及互相转换过程中体现得更为明显,这些差异是其底层实现过程不同所致。结合程序实例的分析可以看出,两种语言在布尔数据类型、字符串处理、指针与引用的参数传递等相关应用中的差异。

关键词： C语言 C++语言实例分析

CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses

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World Journal of Gastrointestinal Oncology 2013年第3期5卷 50-59页

作者： Carlos Alberto Fajardo Andrés Javier Quiroga Andrea Coronado Karen Labrador Nicole Acosta Pilar delgado Carlos Jaramillo mar.a Mercedes Bravo Cancer and Infectious Agents Research Group National Cancer Institute Molecular Diagnostics and Bioinformatics Laboratory Biological Sciences Department Los Andes University

AIM: To investigate the influence of the CagA diversity in Helicobacter pylori (H. pylori ) strains from Colombia on the host cell biology. METHODS: Eighty-four H. pylori-cagA positive strains with different Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs patterns, isolated from patients with gastritis (n=17), atrophic gastritis (n=17), duodenal ulcer (n=16), intestinal metaplasia (n=16) and gastric cancer (n=18), were included. To determine the integrity of the cag pathogenicity island (cag PAI) we evaluated the presence of cagA, cagT, cagE, and cag10 genes by polymerase chain reaction. AGS gastric epithelial cellswere infected with each strain and assayed for translo-cation and tyrosine phosphorylation of CagA by western blot, secretion of interleukin-8 (IL-8) by enzyme-linked immuno sorbent assay after taking supernatants from cocultures and cell elongation induction. For cell elongation quantification, coculture photographs were taken and the proportion of "hummingbird" cells (>15 μm) was determined. RESULTS: Overall 72% (60/84) of the strains were found to harbor a functional cag PAI. Levels of phos-phorylated CagA were significantly higher for isolates from duodenal ulcer than the ones in strains from gas-tritis, atrophic gastritis, intestinal metaplasia and gastric cancer (49.1% ± 23.1% vs 21.1% ± 19.5%, P defective strains did not induce detectable levels of IL-8 at 6 h post-infection. At 30 h post-infection all strains induced IL-8 expression in AGS cells, although cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI (57.1 ± 56.6 pg/mL vs 513.6 ± 338.6 pg/mL,P < 0.0001). We did not observe differences in the extent of cell

关键词： Helicobacter pylori cagA 3’region CagA protein Interleukin 8 Cell elongation Glu-Pro-Ile-Tyr-Ala