检索结果-南通市图书馆

The GSTP1 gene variant rs1695 is not associated with an increased risk of multiple sclerosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2015年第6期12卷 777-779页

作者： Jose AG Agundez Elena Garcia-martin carmen martinez Julian Benito-Leon Julian Benito-Leon Jorge Millan-Pascualmar.a Diaz-Sanchez Patricia Calleja Diana Pisa Laura Turpin-Fenoll Hortensia Alonso-Navarro Lucia Ayuso-Peralta Dolores Torrecillas esteban Garcla-Albea Jose Francisco Plaza-Nieto Felix Javier Jimenez-Jimenez Department of Pharmacology University of Extremadura CAceres Spain Department of Biochemistry and Molecular Biology University of Extremadura Caceres Spain CiBERNED Centro de InvestigaciOn BiomEdica en Red de Enfermedades Neurodegenerativas Instituto de Salud Carlos III Spain Service of Neurology Hospital Universitario Doce de Octubre Madrid Spain Department of Medicine University Complutense Madrid Spain Section of Neurology Hospital La Mancha-Centro Alcazar de San Juan (Ciudad Real) Spain Centro de Biologfa Molecular Severo Ochoa (CSIC-UAM) Facultad de Ciencias Universidad Autonoma Cantoblanco 28049 Madrid Spain Department of Medicine-Neurology Hospital 'Principe de Asturias' Universidad de Alcala Alcala de Henares (Madrid) Spain Section of Neurology Hospital Universitario del Sureste Arganda del Rey (Madrid) Spain Correspondence: Dr FJ Jimenez-Jimenez C/Marroquina 14 3° B E-28030 Madrid Spain.

We analyzed the allelic and genotypic frequencies of the glutathione-S-transferase P1 （GSTPI） rs1695 single nucleotide polymorphism （SNP） in 290 patients with multiple sclerosis （MS） and in 310 healthy controls. We found no significant association between the rs1695 variant and MS. Among MS patients, there was no relationship between the rs1695 variant and either gender, clinical type of MS or the age of onset of MS.

关键词： associated increased sclerosis

Molecular epidemiology and putative origin of hepatitis C virus in random volunteers from Argentina

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第35期19卷 5813-5827页

作者： Noemí del Pino José Raúl Oubia Francisco Rodríguez-Frías Juan Ignacio esteban mar.a Buti Teresa Otero Josep Gregori Damir García-Cehic Silvia Camos mar.a Cubero Rosario Casillas Jaume Guàrdia Rafael esteban Josep Quer Apunlab Laboratories Institute of Medical Microbiology and Parasitology (IMPAM) UBA-CONICET Department of MicrobiologyParasitology and Immunology Faculty of Medicine University of Buenos Aires CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd) del Instituto de Salud Carlos Ⅲ and Biochemistry Department Hospital Universitari Vall d’Hebrón (HUVH) CIBERehd del Instituto de Salud Carlos Ⅲ Autonomous University of Barcelona and Liver Unit Internal Medicine Lab. Malalties Hepatiques Vall d’Hebron Institut de Recerca (VHIR) Hospital Universitari Vall d’Hebrón (HUVH) Unitat de Diagnostic Molecular HUVH Liver Unit Internal Medicine Lab Malalties Hepatiques VHIR-HUVH Roche Diagnostics SL CIBERehd del Instituto de Salud Carlos Ⅲ Liver Unit Internal Medicine Lab MalaltiesHepatiques VHIR-HUVH Liver Unit and Biochemistry Department HUVH

AIM:To study the subtype prevalence and the phylogenetic relatedness of hepatitis C virus(HCV)sequences obtained from the Argentine general population,a large cohort of individuals was ***:Healthy Argentinian volunteers(n=6251)from 12 provinces representing all geographical regions of the country were *** parents or legal guardians of individuals younger than 18 years provided informed written consent for *** corresponding written permission from all municipal authorities was obtained from each city or town where subjects were to be *** RNA reverse transcription-polymerase chain reaction products were sequenced and phylogenetically *** 5’untranslated region(5’UTR)was used for RNA detection and initial genotype *** NS5B polymerase region,encompassing nt 8262-8610,was used for ***:An unexpectedly low prevalence of HCV infection in the general population(0.32%)was *** data contrasted with previous studies that reported rates ranging from 1.5%to 2.5%,mainly performed in selected populations of blood donors or vulnerable *** latter values are in keeping with the prevalence reported by the 2007 Argentinian HCV Consensus(approximately 2%).HCV subtypes weredistributed as follows:1a(25%),1b(25%),2c(25%),3a(5%),and 2j(5%).Two isolates ascribed either to genotype 1(5%)or to genotype 3(5%)by 5’UTR phylogenetic analysis could not be *** 1a sequences comprised a highly homogeneous population and clustered with United States ***1b sequences represented a heterogeneous population,suggesting that this genotype might have been introduced from different *** subtype 2c sequences clustered close to the 2c reported from Italy and Southern ***:HCV has a low prevalence of 0.32%in the studied general population of *** pattern of HCV introduction and transmission in Argentina appears to be a consequence of multiple events and different

关键词： Hepatitis C virus NS5B subtyping Molecular epidemiology Hepatitis C virus Argentina Hepatitis C virus 5’ untranslated region

Anterior Segment Parameters Associated to Acute Glaucoma with Slit Lamp Optical Coherence Tomography

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Ophthalmology 2014年第2期4卷 40-45页

作者： Liamet Fernández Argones Ibrain Piloto Díaz Sirley Sibello Deustua carmen mar.a Padilla González Glaucoma Department Cuban Ophthalmology Institute “Ramón Pando Ferrer” Havana City Cuba

Purpose: To identify the anterior segment parameters associated to acute primary angle closure. Patients and Methods: Case-control study in 58 primary angle closure suspect eyes (PACS, control group) and in 19 acute primary angle closure fellow eyes, case group. All subjects underwent SL OCT imaging in a dark room. Results: The SS, ACD and ACV measures were significantly smaller in case group than in control group (p = 0.011;p mary angle closure is associated to the smaller anterior segment dimensions and the thicker iris compared to PACS.

关键词： Acute Primary Angle Closure Acute Glaucoma Angle Opening Distance Trabecular-Iris Space Area Iris Thickness Optical Coherence Tomography

Conservation and variability of hepatitis B core at different chronic hepatitis stages

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2020年第20期26卷 2584-2598页

作者： mar.al Yll mar.a Francesca Cortese Mercedes Guerrero-Murillo Gerard Orriols Josep Gregori Rosario Casillas Carolina González Sara Sopena Cristina Godoy mar.a Vila David Tabernero Josep Quer Ariadna Rando Rosa Lopez-martinez Rafael esteban mar Riveiro-Barciela mar.a Buti Francisco Rodríguez-Frías Liver Pathology Unit Departments of Biochemistry and MicrobiologyHospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Liver Unit Liver Disease Laboratory-Viral HepatitisVall d'Hebron Institut Recerca-Hospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Department of Microbiology Hospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas Instituto de Salud Carlos ⅢMadrid 28029Spain Liver Unit Department of Internal MedicineHospital Universitari Vall d'HebronUniversitat Autónoma de BarcelonaBarcelona 08035Spain

BACKGROUND Since it is currently not possible to eradicate hepatitis B virus(HBV)infection with existing treatments,research continues to uncover new therapeutic *** core protein,encoded by the HBV core gene(HBC),intervenes in both structural and functional processes,and is a key protein in the HBV life *** this reason,both the protein and the gene could be valuable targets for new therapeutic and diagnostic ***,alterations in the protein sequence could serve as potential markers of disease *** To detect,by next-generation sequencing,HBC hyper-conserved regions that could potentially be prognostic factors and targets for new *** Thirty-eight of 45 patients with chronic HBV initially selected were included and grouped according to liver disease stage[chronic hepatitis B infection without liver damage(CHB,n=16),liver cirrhosis(LC,n=5),and hepatocellular carcinoma(HCC,n=17)].HBV DNA was extracted from patients’plasma.A region between nucleotide(nt)1863 and 2483,which includes HBC,was amplified and analyzed by next-generation sequencing(Illumina Mi Seq platform).Sequences were genotyped by distance-based discriminant *** and intergroup nt and amino acid(aa)conservation was determined by sliding window *** presence of nt insertion and deletions and/or aa substitutions in the different groups was determined by aligning the sequences with genotype-specific consensus *** Three nt(nt 1900-1929,2249-2284,2364-2398)and 2 aa(aa 117-120,159-167)hyper-conserved regions were shared by all the clinical *** groups showed a similar pattern of conservation,except for five nt regions(nt 1946-1992,2060-2095,2145-2175,2230-2250,2270-2293)and one aa region(aa 140-160),where CHB and LC,respectively,were less conserved(P<0.05).Some group-specific conserved regions were also observed at both nt(2306-2334 in CHB and 1935-1976 and 2402-2435 in LC)and aa(between aa 98-103 in CHB and 28-30 and 5

关键词： Hepatitis B virus Hepatitis B core gene Next-generation sequencing Genetic conservation Amino acid substitution Gene therapy Small interfering RNA

Reduction of the closure time of postoperative enterocutaneous fistulas with fibrin sealant

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第22期16卷 2793-2800页

作者： Jorge Avalos-González Eliseo Portilla-deBuen Caridad Aurea Leal-Cortés Abel Orozco-Mosqueda mar.a del carmen Estrada-Aguilar Gabriela Abigail Velázquez-Ramírez Gabriela Ambriz-González Clotilde Fuentes-Orozco Aldo Emmerson Guzmán-Gurrola Alejandro González-Ojeda Research Unit in Clinical Epidemiology High Specialized Medical Unit Surgical Research Division National Western Medical Center Department of Surgery Regional General Hospital #1 of the Mexican Institute for Social Security Avenida Héroes de Nocupetaro y Nicolás de Regules Colonia Centro Department of Pediatric Surgery Children's Hospital National Western Medical Center

AIM: To assess whether the use of fibrin sealantshortens the closure time of postoperative enterocutaneous fistulas (ECFs). METHODS: The prospective case-control study included 70 patients with postoperative ECFs with an output of 2 cm and without any local complication. They were divided into study (n = 23) and control groups (n = 47). Esophageal, gastric and colocutaneous fistulas were monitored under endoscopic visualization, which also allowed fibrin glue application directly through the external hole. Outcome variables included closure time, time to resume oral feeding and morbidity related to nutritional support. RESULTS: There were no differences in mean age, fistula output, and follow-up. Closure-time for all patients of the study group was 12.5 ± 14.2 d and 32.5 ± 17.9 d for the control group (P < 0.001), and morbidity related to nutritional support was 8.6% and 42.5%, respectively (P < 0.01). In patients with colonic fistulas, complete closure occurred 23.5 ± 19.5 d after the first application of fibrin glue, and spontaneous closure was observed after 36.2 ± 22.8 d in the control group (P = 0.36). Recurrences were observed in 2 patients because of residual disease. One patient of each group died during follow-up as a consequence of septic complications related to parenteral nutrition. CONCLUSION: Closure time was significantly reduced with the use of fibrin sealant, and oral feeding was resumed faster. We suggest the use of fibrin sealant for the management of stable enterocutaneous fistulas.

关键词： Enterocutaneous fistulas Fibrin sealant Spontaneous closure

Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Microbiology 2016年第9期6卷 733-744页

作者： Adriana Beatriz Simes Ramona del carmen Maturano Valentín Tassile Adriana Catalina Caballero Silvana mar.a del Mónaco Facultad de Ciencias y Tecnología de los Alimentos Universidad Nacional del Comahue (UNCo) Villa Regina Argentina Grupo de Enología Instituto de Investigación y Desarrollo en Ingeniería de Procesos Biotecnología y Energías Alternativas (PROBIEN-Consejo Nacional de Investigaciones Científicas y Técnicas CONICET) Facultad de Ingeniería Universidad Nacional del Comahue (UNCo) Neuquén Argentina

At present, Argentina does not count with a production of indigenous yeast strains with suitable technological and oenological features to be used in the regional winery industry. Isolation and molecular characterization of these microorganisms and its fermentation attributes would be relevant to the sustainable development of the activity in the country and to recognize and preserve the biodiversity of the region. Eight strains isolated from grapes and musts from the North Patagonian region and genetically identified as Saccharomyces cerevisiae, were studied for their fermentation behavior, emphasizing in hexose transport through the plasma membrane, which is the limiting step of the process. Thus, sugar consumption profiles were analyzed in different media at laboratory scale, to be subsequently applied to the fermentation of natural musts. Three of the eight initial strains were selected, named NNM10, NIF8 and NMN16 according to their fermentation profiles. The expression of hexose transporters during fermentations revealed interesting differences in the response of each strain to sugar consumption, where transporters HXT2 and HXT5 showed significant changes in expression in Patagonian strains, which are normally associated to endurance to culture stress conditions. The results obtained by combining the characteristics studied, at molecular and physiological level, are extremely encouraging. Native strain NMN16, showed a high potential for application in local winemaking. Assays carried out on a pilot scale will determine the feasibility of applying this strain with promising technological features at industrial scale.

关键词： Fermentation Wine Saccharomyces cerevisiae Hexose Transporters

The Relationship between Consumption,Socioeconomic Level and Reasons of Tomato Intake in Mexico

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences 2018年第7期9卷 777-791页

作者： mar.ana Lares-Michel Fatima Ezzahra Housni Virginia Gabriela Aguilera Cervantes Rosa mar.a Michel Nava Humberto Bracamontes Del Toro Claudia Llanes Canedo mar.a del carmen Barragan Carmona Research Center in Food Behavior and Nutrition(CICAN) Centro Universitario del SurUniversidad de GuadalajaraGuzmanMexico Tecnologico Nacional de Mexico GuzmanMexico

The red tomato has been associated to Mexico throughout history. This vegetable is native to the New World and was domesticated in Mesoamerica, before its arrival to Europe. It has always been of great cultural importance in Mexicans diet and of great economic value. Currently, Mexico is the first tomato exporter in the world. However, its marketing affects its price and availability and can harm consumption. Then, due to the cultural role of this vegetable, it is interesting to analyze the relationship between consumption, socioeconomic status and tomato intake reasons in the case of Zapotlan el Grande, Jalisco, Mexico. For this reason, a Food Consumption Frequency Questionnaire (FCFQ) was applied to 384 adults from 18 to 65 years old, which included classification of socioeconomic level and consumption reasons. The results showed that the tomato was ingested by more than 90% of the population, with an average frequency of 21.66 ± 10.57 times per month and 114.68 daily grams per capita. For the relationship between socioeconomic level and intake reasons, it was found that there was an impact of monthly income, occupation and gastronomy on the intake of this vegetable.

关键词： Tomato Consumption Socioeconomic Level Motives of Consumption Zapotlan El Grande

Pathology handling of pancreatoduodenectomy specimens:Approaches and controversies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2014年第9期6卷 351-359页

作者： mar.a del carmen Gómez-Mateo Luis Sabater-Ortí Antonio Ferrández-Izquierdo Department of Pathology Hospital Universitario Donostia Department of General and Gastroenterological Surgery Hospital Clínico ValenciaUniversity of Valencia Department of Pathology Hospital Clínico ValenciaUniversity of Valencia

Pancreatic cancer,with a 5% 5-year survival rate,is the fourth leading cause of cancer death in Western ***,only 20% of all patients benefit from surgical *** need to prolong survival has prompted pathologists to develop improved protocols to evaluate pancreatic specimens and their surgical ***,the new protocols will provide clinicians with more powerful prognostic indicators and accurate information to guide their therapeutic *** the availability of several guidelines for the handling and pathology reporting of duodenopancreatectomy specimens and their continual updating by expert pathologists,there is no consensus on basic issues such as surgical margins or the definition of incomplete excision(R1) of pancreatic ductal *** article reviews the problems and controversies that dealing with duodenopancreatectomy specimens pose to pathologists,the various terms used to define resection margins or infiltration,and *** reviewing the literature,including previous guidelines and based on our own experience,we present our protocol for the pathology handling of duodenopancreatectomy specimens.

关键词： handling consensus powerful infiltration reviewing updating incomplete margin availability dealing

Organic Carbon Storage in Evergreen Oak Forest Ecosystems of the Middle and High Moroccan Atlas Areas

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Forestry 2015年第3期5卷 260-273页

作者： Mohamed Boulmane mar.a del carmen Santa-Regina Mohamed Halim Abderrahim Khia Hayat Oubrahim Hassan Abbassi Ignacio Santa-Regina Forest Research Center B.P. 763 Av. Omar Ibn Khattab Agdal Morocco Faculty of Biology Campus Miguel de Unamuno C/Donantes de Sangre s/n Salamanca Spain Faculty of Sciences Mohamed V Agdal Morocco Faculty of Sciences Ibn Tofail Kénitra Morocco Institute of Natural Resources and Agrobiology of Salamanca IRNASA-CSIC Salamanca Spain

We report carbon stock in biomass, litter and soil estimated for six locations in natural Quercus ilex L. stands of the Middle and High Moroccan Atlas. Twenty trees at each location were selected according to their diameter classes and felled to measure the biomass of trunk, branches, twigs and leaves and determine allometric relationships. Soil was sampled in five depths (0 - 15, 15 - 30, 30 - 50, 50 - 70 and 70 - 100 cm) and litterfall production measured in all tree stands. The total carbon stock in above-ground biomass ranged between 17 Mg·haǃ in Aït Aamar stand (High Atlas) and 91 Mg·haǃ in Ksiba stand (Middle Atlas). Perennial organs (trunk, branches and twigs) stored over 95% of the tree carbon stock. Soil organic carbon concentrations ranged from 0.01% (in 70 - 100 cm in all stands) to 8.1% (in 0 - 15 cm in the Ajdir stand in Middle Atlas). The total organic carbon stock in the soil ranged between 141.4 t·haǃ in Ajdir and 24.6 t·haǃ in Asloul. The litter contained 0.2 Mg C haǃ in the clearing (C2) stand of High Atlas and 14.3 Mg C haǃ in (Ajdir) of carbon. The best fitted model for predicting carbon stock in tree biomass was obtained by applying the allometric equation Y = aXb for each biomass fraction and stand, where Y is the aboveground biomass (dry weight) and X is the DBH (Mean diameter at breast height, 1.30 m). These previous data obtained in the present study confirm the important function of these natural forests as longterm C sinks, in forest biomass, litter and soil. The potential long term C storage of these systems is moderately high, especially in less-intensively managed forests that include large trees. The established relationship between DBH and carbon stock in different tree organs can be used for forest carbon accounting, and also synthesize available information on oak forest as a sink for atmospheric CO2, and identify the management options that may enhance the capacity for C capture/ storage

关键词： Allometric Methods Bulk Density Soil Organic Carbon Vegetation