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Role of hypoxia preconditioning in therapeutic potential of mesenchymal stem-cell-derived extracellular vesicles

World Journal of Stem Cells 2022年第7期14卷 453-472页

作者： Victoria Pulido-Escribano Bárbara Torrecillas-Baena mar.a Camacho-Cardenosa Gabriel dorado mar.aÁngeles Gálvez-Moreno Antonio Casado-díaz Unidad de Gestión Clínica de Endocrinología y nutrición-GC17 Instituto Maimónides de Investigación Biomédica de CórdobaHospital Universitario Reina SofíaCórdoba 14004Spain dep.Bioquímica y Biología Molecular Campus Rabanales C6-1-E17Campus de Excelencia Internacional Agroalimentario(ceiA3)Universidad de CórdobaCIBERFESCórdoba 14071Spain

The use of mesenchymal stem-cells(MSC)in cell therapy has received considerable attention because of their *** properties include high expansion and differentiation in vitro,low immunogenicity,and modulation of biological processes,such as inflammation,angiogenesis and ***,the regenerative effect of MSC is partly due to their paracrine *** has prompted numerous studies,to investigate the therapeutic potential of their secretome in general,and specifically their extracellular vesicles(EV).The latter contain proteins,lipids,nucleic acids,and other metabolites,which can cause physiological changes when released into recipient ***,contents of EV can be modulated by preconditioning MSC under different culture *** them,exposure to hypoxia stands out;these cells respond by activating hypoxia-inducible factor(HIF)at low O_(2) *** has direct and indirect pleiotropic effects,modulating expression of hundreds of genes involved in processes such as inflammation,migration,proliferation,differentiation,angiogenesis,metabolism,and cell *** of these genes is reflected in the contents of secreted ***,numerous studies show that MSC-derived EV conditioned under hypoxia have a higher regenerative capacity than those obtained under *** this review,we show the implications of hypoxia responses in relation to tissue *** addition,hypoxia preconditioning of MSC is being evaluated as a very attractive strategy for isolation of EV,with a high potential for clinical use in regenerative medicine that can be applied to different pathologies.

关键词： Cell priming Extracellular vesicles Hypoxia Hypoxia-inducible factor Mesenchymal stemcells Regenerative medicine

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Bacterial diversity in Fez tanneries and Morocco's Binlamdoune River,using 16S RnA gene based fingerprinting

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Journal of Environmental Sciences 2010年第12期22卷 1944-1953页

作者： Adil Essahale Moustafa Malki Irma marín Mohieddine Moumni Moulay Isml University Faculty of ScienceDepartment of BiologyBP 11 201 ZitouneMeknès 50000Morocco department of Molecular Biology Faculty of ScienceAutonomous University of Madrid (UAM)

Tannery wastewater causes serious ecological and sanitary damage. Chemical analysis of water from Binlamdoune River of the medina of Fez was conducted and the results revealed the presence of toxic elements from tanneries and other industrial activities, which strongly affected water quality. To determine the effectiveness of bioremediation for depollution, we studied the abundance and diversity of bacteria residing in these polluted environments. Conducting denaturing gradient gel electrophoresis （PCR-dGGE） of the 16S rdnA area using primers related to bacteria showed a bacterial community belonging to eubacterial groups, that is, Epsilonproteobacteria, Clostridia, Lactobacillales, Bacteroidetes, Gammaproteobacteria, and Alphaproteobacteria. In addition, cloning displayed the presence of clones belonging to the Firmicutes group. Moreover, scanning electron microscopy revealed a significant heterogeneity of microorganism forms and structures. These endogenous microbes could have a significant role in the purification of Binlamdoune River and Fez tannery wastewater.

关键词： Fez tanneries Binlamdoune River biodiversity bacteria 16S rRnA

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Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates

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World Journal of Gastroenterology 2010年第3期16卷 320-329页

作者： maritza Lienlaf Juan Pablo Morales mar.a Inés díaz Rodrigo díaz Elsa Bruce Freddy Siegel Gloria León Paul R Harris Alejandro Venegas Microbial Pathogenesis and Vaccine Biotechnology Laboratory Department of Molecular Genetics and MicrobiologyFaculty of Biological SciencesPontificia Universidad Católica de Chile Gastroenterology Unit Faculty of MedicineUniversidad Austral de Chile Institute of Biochemistry Faculty of SciencesUniversidad Austral de Chile department of Pediatrics School of MedicinePontificia Universidad Católica de Chile

AIM:To evaluate how widely Helicobacter pylori (H. pylori ) HopE and HopV porins are expressed among Chilean isolates and how seroprevalent they are among infected patients in ***: H. pylori hopE and hopV genes derived from strain CHCTX-1 were cloned by polymerase chain reaction (PCR), sequenced and expressed in Escherichia coli Ad494 (dE3). Gel-purified porins were used to prepare polyclonal antibodies. The presence of both genes was tested by PCR in a collection of H. pylori clinical isolates and their expression was detected in lysates by immunoblotting. Immune responses against HopE, HopV and other H. pylori antigens in sera from infected and non-infected patients were tested by Western blotting using these sera as f irst antibody on recombinant H. pylori ***: PCR and Western blotting assays revealed that 60 and 82 out of 130 Chilean isolates carried hopE and hopV genes, respectively, but only 16 and 9, respectively, expressed these porins. IgG serum immunoreactivity evaluation of 69 H. pylori-infected patients revealed that HopE and HopV were infrequently recognized (8.7% and 10.1% respectively) compared to H. pylori VacA (68.1%) and CagA (59.5%) antigens. Similar values were detected for IgA serum immunoreactivity against HopE (11.6%) and HopV (10.5%) although lower values for VacA (42%) and CagA (17.4%) were obtained when compared to the IgG ***: A scarce expression of HopE and HopV among Chilean isolates was found, in agreement with the infrequent seroconversion against these antigens when tested in infected Chilean patients.

关键词： Helicobacter pylori Gene expression HopE and HopV porins Antigens Immune response

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Role of lipids in the control of autophagy and primary cilium signaling in neurons

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neural Regeneration Research 2024年第2期19卷 264-271页

作者： mar.a Paz Hernández-Cáceres daniela Pinto-nuñez Patricia Rivera Paulina Burgos Francisco díaz-Castro Alfredo Criollo mar.a Jose Yañez Eugenia Morselli Instituto de Investigación en Ciencias Odontológicas(ICOd) Facultad de OdontologíaUniversidad de ChileSantiagoChile department of Basic Sciences Faculty of Medicine and ScienceUniversidad San SebastiánSantiagoChile Physiology department Faculty of Biological SciencesPontificia Universidad Católica de ChileSantiagoChile Advanced Center for Chronic diseases(ACCdiS) Facultad de Ciencias Químicas y Farmacéuticas&Facultad de MedicinaUniversidad de ChileSantiagoChile Autophagy Research Center SantiagoChile

The brain is,after the adipose tissue,the organ with the greatest amount of lipids and diversity in their composition in the human *** neurons,lipids are involved in signaling pathways controlling autophagy,a lysosome-dependent catabolic process essential for the maintenance of neuronal homeostasis and the function of the primary cilium,a cellular antenna that acts as a communication hub that transfers extracellular signals into intracellular responses required for neurogenesis and brain development.A crosstalk between primary cilia and autophagy has been established;however,its role in the control of neuronal activity and homeostasis is barely *** this review,we briefly discuss the current knowledge regarding the role of autophagy and the primary cilium in *** we review the recent literature about specific lipid subclasses in the regulation of autophagy,in the control of primary cilium structure and its dependent cellular signaling in physiological and pathological conditions,specifically focusing on neurons,an area of research that could have major implications in neurodevelopment,energy homeostasis,and neurodegeneration.

关键词： autophagic flux cholesterol fatty acids GPCR lysosomal storage diseases neurons nPC1 phosphoinositides primary cilium

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Current and emerging therapies for alcohol-associated hepatitis

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Liver Research 2023年第1期7卷 35-46页

作者： Francisco Idalsoaga Gustavo Ayares Luis Antonio díaz Jorge Arnold mar.a Ayala-Valverde david Hudson marco Arrese Juan Pablo Arab department of Gastroenterology School of MedicinePontificia Universidad Catolica de ChileSantiagoChile Internal Medicine Service Hospital El PinoCritical Patient UnitClinica DavilaSantiagoChile division of Gastroenterology Department of MedicineSchulich School of MedicineWestern University&London Health Sciences CentreLondonOntarioCanada department of Epidemiology and Biostatistics Schulich School of MedicineWestern UniversityLondonOntarioCanada Alimentiv LondonOntarioCanada

Alcohol-related liver disease(ALd)encompasses a spectrum of diseases caused by excessive alcohol *** includes hepatic steatosis,steatohepatitis,variable degrees of fibrosis,cirrhosis,and alcohol-associated hepatitis(AH),the latter being the most severe acute form of the *** AH is associated with high mortality(reaching up to 30%e50%)at 90 *** cornerstone of ALd,and particularly AH,treatment continues to be abstinence,accompanied by support measures such as nutritional supplementation and management of alcohol withdrawal syndrome(AWS).In severe AH with model for end-stage liver disease(MELd)score21,corticosteroids can be used,especially MELd score between 25 and 39,where the highest benefit is *** key aspects of treatment include the early identification of infections and their associated management and the proper identification of potential candidates for liver *** development of new therapies based on the pathophysiology and mechanisms of liver injury are *** includes the modulation and management of the innate immune response,gut dysbiosis,bacterial translocation,and bacteria-derived products from the *** hold promise for the future of AH treatment.

关键词： Alcohol-associated hepatitis(AH) Alcohol-related liver disease(ALd) Alcohol use disorder(AUd) AUd treatment nutritional therapy Steroids therapy Cirrhosis Fibrosis

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different xylogenesis responses to atmospheric water demand contribute to species coexistence in a mixed pine–oak forest

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Journal of Forestry Research 2023年第1期34卷 51-62页

作者： marín Pompa-García J.Julio Camarero Michele Colangelo Facultad de Ciencias Forestales y Ambientales de la Universidad Juárez del Estado de durango Río Papaloapan y Blvd.Durango S/N Col.Valle del SurDurangoMéxico Instituto Pirenaico de Ecología(IPE-CSIC) Avda.Montanna 1005ZaragozaSpain School of Agricultural ForestFood and Environmental SciencesUniv.BasilicataPotenzaItaly

Seasonal patterns of wood formation(xylogenesis)remain understudied in mixed pine-oak forests despite their contribution to tree coexistence through temporal niche *** was assessed in three pine species(Pinus cembroides,Pinus leiophylla,Pinus engelmannii)and one oak(Quercus grisea)coexisting in a semi-arid Mexican *** main xylogenesis phases(production of cambium cells,radial enlargement,cell-wall thickening and maturation)were related to climate data considering 5-15-day temporal *** pines,cambium activity maximized from mid-march to April as temperature and evaporation increased,whereas cell radial enlargement peaked from April to May and was constrained by high evaporation and low ***-wall thickening peaked from June to July and in August-September as maximum temperature and vapour pressure deficit(VPd)*** of earlywood and latewood tracheids occurred in May-June and June-July,enhanced by high minimum temperatures and VPd in *** and *** oak,cambial onset started in march,constrained by high minimum temperatures,and vessel radial enlargement and radial increment maximized in April as temperatures and evaporation increased,whereas early wood vessels matured from May to June as VPd ***,15-day wet conditions enhanced cell radial enlargement in *** and ***,whereas early-summer high 15-day temperature and VPd drove cell-wall thickening in *** night conditions and high evaporation rates during spring and summer enhanced *** earlier growth peak in oak and a higher responsiveness to spring-summer water demand in pines contributed to their coexistence.

关键词： Bimodal growth drought Vapour pressure deficit Wood formation Xylem phenology

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Small intestinal bacterial overgrowth in inactive Crohn's disease:Influence of thiopurine and biological treatment

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World Journal of Gastroenterology 2014年第38期20卷 13999-14003页

作者： Cristina Sánchez-Montes Vicente Ortiz Guillermo Bastida Ester Rodríguez mar.a Yago belén beltrán mar.am Aguas marisa Iborra Vicente Garrigues Julio Ponce Pilar nos Gastroenterology Unit Hospital Universitari i Politécnic La Fe

AIM: To investigate the influence of thiopurines and biological drugs on the presence of small intestinal bacterial overgrowth (SIBO) in patients with inactive Crohn’s disease (Cd).

关键词： Crohn’ s disease Bacterial overgrowth Thiopurines Biologics Inflammatory bowel disease

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Patients with hepatocellular carcinoma that die during the first year of liver transplantation have high blood sFasL concentrations

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World Journal of Clinical Cases 2023年第8期11卷 1753-1760页

作者： Leonardo Lorente Sergio T Rodriguez Pablo Sanz Agustín F González-Rivero Antonia Pérez-Cejas Javier Padilla dácil díaz Antonio González mar.a M martín Alejandro Jiménez Purificación Cerro Julián Portero Manuel A Barrera Intensive Care Unit Hospital Universitario de CanariasLa Laguna 38320Spain Intensive Care Unit Hospital Universitario Nuestra Señora CandelariaSanta Cruz Tenerife 38010Spain department of Surgery Hospital Universitario Nuestra Señora de CandelariaSanta Cruz Tenerife 38010Spain department of Laboratory Hospital Universitario de CanariasLa Laguna 38320Spain department of digestive Hospital Universitario Nuestra Señora de CandelariaSanta Cruz Tenerife 38010Spain Research Unit Hospital Universitario de CanariasLa Laguna 38320Spain Transplant Unit Hospital Universitario Nuestra Señora CandelariaSanta Cruz Tenerife 38010Spain department of Radiology Hospital Universitario Nuestra Señora CandelariaSanta Cruz Tenerife 38010Spain

BACKGROUnd Fas ligand(FasL)is one ligand that activates extrinsic apoptosis *** expression in lymphocytes of FasL have been found in patients with acute rejection of liver transplantation(LT).no high blood concentrations of soluble FasL(sFasL)have been found in patients with acute LT rejection;however,the samples size of those studies was *** To determine whether patients with hepatocellular carcinoma(HCC)that dead during the first year of LT have higher blood sFasL concentrations previously to LT that those who that remain alive in a study of higher sample *** Patients underwent LT due to HCC were included in this retrospective *** sFasL levels prior to LT were measured and one-year LT mortality was *** non-surviving patients(n=14)showed higher serum sFasL levels[477(269-496)vs 85(44-382)pg/mL;Pn surviving patients(n=113).Serum sFasL levels(pg/mL)were associated with mortality(OR=1.006;95%CI=1.003-1.010;P=0.001)independently of age of LT donor in the logistic regression *** We report for the first time that HCC patients who die within the first year of HT have higher blood sFasL concentrations prior to HT than those who remain alive.

关键词： sFasL Hepatocellular carcinoma Liver transplantation Mortality Outcome

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nuclear Activity in Circumnuclear Ring Galaxies

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International Journal of Astronomy and Astrophysics 2016年第3期6卷 219-235页

作者： mar.a P. Agüero Rubén J. díaz Horacio dottori Observatorio Astronómico de Córdoba UNCand CONICET Córdoba Argentina ICATE CONICET San Juan Argentina Gemini Observatory La Serena Chile Instituto de Física UFRGS Porto Alegre Brazil

We have analyzed the frequency and properties of the nuclear activity in a sample of galaxies with circumnuclear rings and spirals (CnRs), compiled from published data. From the properties of this sample a typical circumnuclear ring can be characterized as having a median radius of 0.7 kpc (mean 0.8 kpc, rms 0.4 kpc), located at a spiral Sa/Sb galaxy (75% of the hosts), with a bar (44% weak, 37% strong bars). The sample includes 73 emission line rings, 12 dust rings and 9 stellar rings. The sample was compared with a carefully matched control sample of galaxies with very similar global properties but without detected circumnuclear rings. We discuss the relevance of the results in regard to the AGn feeding processes and present the following results: 1) bright companion galaxies seem not to be important for the appearance of CnRs, which appear to be more related to intrinsic properties of the host galaxies or to minor merger processes;2) the proportion of weak bars in galaxies with a CnR is higher than expected;3) the incidence of Seyfert (Sy) activity coeval with CnRs is significantly larger than the rate expected from the morphological distribution of the host galaxies;4) the rate of Sy 2 to Sy 1 type galaxies with CnRs is about three times larger than the expected ratio for galaxies without CnRs and is opposite to that predicted by the geometric paradigm of the classical unified model for AGns, although it does support the hypothesis that Sy 2 activity is linked to circumnuclear star formation. The possible selection effects of the sample are discussed, and we conclude that the detected trends are strong enough to justify high quality observations of as large as possible set of galaxies with circumnuclear rings and their matched control samples.

关键词： Galaxies Spiral nuclei Structure dynamics Active

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Calibration of the dEM sliding friction and rolling friction parameters of a cohesionless bulk material

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Particuology 2024年第9期92卷 126-139页

作者： Jonatan marín Pérez Tugrul Comlekci Yevgen Gorash donald MacKenzie University of Strathclyde 75 Montrose StreetG11XJGlasgowUnited Kingdom

The discrete element method(dEM)has become a valuable tool for understanding the mechanical behaviour of granular assemblies,however,the accuracy of the dEM simulations depends on several interaction parameters such as the sliding friction and rolling friction ***,these parameters are estimated using bulk calibration approach(BCA)where the draw down test has been suggested as an effective way to approach these *** test provides up to four bulk criteria,the angle of repose,shear angle,mass flow rate and the mass loss which are used to narrow down the possible ***,there is still more research needed around it to fully understand how this methodology *** experimental and numerical study was carried out using the draw down test to assess the influence of different mass flow rates and particle shapes on dEM parameters for a cohesionless bulk *** is concluded that use of multi-sphere particles and three aperture sizes in the draw down test to calibrate the sliding and rolling friction parameters of a cohesionless bulk material can converge to a small feasible region in which a single combination of the friction coefficients can be *** calibrated sliding and rolling friction coefficients were validated using multi-sphere particles,where the maximum deviation was 5.9%from the experimental values.

关键词： discrete element method Calibration draw down Sliding friction Rolling friction Particle shape

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