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Screen-printed graphite electrode on polyvinyl chloride and parchment strips integrated with genetic programming for in situ nitrate sensing of aquaponic pond water

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Information Processing in Agriculture 2024年第2期11卷 187-201页

作者： Ronnie concepcionⅡ Bernardo Duarte maria Gemel Palconit Jonah Jahara BaunArgel Bandala Ryan Rhay Vicerra Elmer Dadios Department of Manufacturing Engineering and Management De La Salle UniversityManila 1004 Philippines marE-marine and Environmental Sciences Centre.&ARNET-Aquatic Research Infrastructure Network Associated Laboratory Faculdade de Ciencias da Universidade de LisboaCampo Grande1749-016 LisboaPortugal Departamento de Biologia Vegetal da Faculdade de Ciencias da Universidade de Lisboa Campo Grande 1749-016 LisboaPortugal Department of Electronics and Computer Engineering De La Salle UniversityManila 1004 Philppines

Nitrate is the primary water-soluble macronutrient essential for plant growth that is converted from excess fish feeds,fish effluents,and degrading biomaterials on the aquaponic pond floor,and when aquacultural malpractices occur,large amounts of it retain in the water system causing increase rate in eutrophication and toxifies fish and aquaculture *** nitrate sensor prototypes still require performing the additional steps of water sample deionization and dilution and were constructed with expensive *** response to the challenge of sensor enhancement and aquaponic water quality monitoring,this study developed sensitive,repeatable,and reproducible screen-printed graphite electrodes on polyvinyl chloride and parchment paper substrates with silver as electrode material and 60:40 graphite powder:nail polish formulated conductive ink for electrical traces,integrated with 9-gene genetic expression model as a function of peak anodic current and electrochemical test time for nitrate concentration prediction that is embedded into low-power Arduino ESP32 for in situ nitrate sensing in aquaponic pond *** SPE electrical traces were designed on the two types of *** electron microscopy with energy dispersive X-ray confirmed the electrode surface *** cyclic voltammetry using 10 to 100 mg/L KNO3 and water from three-depth regions of the actual pond established the electrochemical test time(10.5 s)and electrode potential(0.135 V)protocol necessary to produce peak current that corresponds to the strength of nitrate ions during *** findings from in situ testing revealed that the proposed sensors have strong linear predictions(R2=0.968 MSE=1.659 for nSPEv and R2=0.966 MSE=4.697 for nSPEp)in the range of 10 to 100 mg/L and best detection limit of 3.15μg/L,which are comparable to other sensors of more complex *** developed three-electrode electrochemical nitrate sensor confirms that it is reliable for bot

关键词： Aquaponic water quality Electrochemical technology Genetic programming Graphite electrode Nitrate sensor Precision agriculture Printed electronics Scanning electron microscopy Screen-printed electrode Voltammetry

A comprehensive review of genetic causes of obesity

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World Journal of Pediatrics 2024年第1期20卷 26-39页

作者： marcio Jose concepcion-Zavaleta Juan Eduardo Quiroz-Aldave maria del Carmen Durand-Vasquez Elman Rolando Gamarra-Osorio Juan del Carmen Valencia de la Cruz Claudia Mercedes Barrueto-Callirgos Susan Luciana Puelles-Leon Elena de Jesus Alvarado-Leon Frans Leiva-Cabrera Francisca Elena Zavaleta-Gutierrez Luis Alberto concepcion-Urteaga Jose Paz-Ibarra Universidad Cientifica del Sur Bolivar 2150 AvenuePueblo Libre15084LimaPeru Division of Medicine Hospital de Apoyo ChepénChepenPeru Division of Family Medicine Hospital de Apoyo ChepénChepenPeru Division of Endocrinology Hospital Victor Lazarte EchegarayTrujilloPeru Division of Pediatrics Hospital de Apoyo ChepenChepenPeru Division of Internal Medicine Hospital de Apoyo ChepenChepenPeru Division of Genetics Universidad Nacional de TrujilloTrujilloPeru Division of Neonatology Hospital Belen de TrujilloTrujilloPeru Division of Internal Medicine Universidad Nacional de TrujilloTrujilloPeru Department of Medicine School of MedicineUniversidad Nacional Mayor de San MarcosLimaPeru

Background Obesity is a multifactorial chronic disease with a high,increasing worldwide *** causes account for 7%of the cases in children with extreme *** sources This narrative review was conducted by searching for papers published in the PubMed/MEDLINE,Embase and SciELO databases and included 161 *** search used the following search terms:"obesity","obesity and genetics","leptin","Prader-Willi syndrome",and"melanocortins".The types of studies included were systematic reviews,clinical trials,prospective cohort studies,cross-sectional and prospective studies,narrative reviews,and case *** The leptin-melanocortin pathway is primarily responsible for the regulation of appetite and body ***,several important aspects of the pathophysiology of obesity remain *** causes of obesity can be grouped into syndromic,monogenic,and polygenic causes and should be assessed in children with extreme obesity before the age of 5 years,hyperphagia,or a family history of extreme obesity.A microarray study,an analysis of the melanocortin type 4 receptor gene mutations and leptin levels should be performed for this *** are three therapeutic levels:lifestyle modifications,pharmacological treatment,and bariatric *** Genetic study technologies are in constant development;however,we are still far from having a personalized approach to genetic causes of obesity.A significant proportion of the affected individuals are associated with genetic causes;however,there are still barriers to its approach,as it continues to be underdiagnosed.

关键词： Leptin Melanocortin Obesity Prader-Willi syndrome Precision medicine

Plant geranylgeranyl diphosphate synthases: every (gene) family has a story

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aBIOTECH 2021年第3期2卷 289-298页

作者： M.Victoria Barja Manuel Rodriguez-concepcion Centre for Research in Agricultural Genomics(CRAG)CSIC-IRTA-UAB-UB Campus UAB Bellaterra08193 Barce-lonaSpain Institute for Plant Molecular and Cell Biology(BMCP) CSIC-Universitat Politecnica de Valencia46022 ValenciaSpain

Plant isoprenoids(also known as terpenes or terpenoids)are a wide family of primary and secondary metabolites with multiple *** particular,most photosynthesis-related isoprenoids(including carotenoids and chlorophylls)as well as diterpenes and polyterpenes derive from geranylgeranyl diphosphate(GGPP)produced by GGPP synthase(GGPPS)enzymes in several cell *** genomes typically harbor multiple copies of differentially expressed genes encoding GGPPS-like *** sequence comparisons allow to identify potential GGPPS candidates,experimental evidence is required to ascertain their enzymatic activity and biologi cal ***,functional analyses of the full set of potential GGPPS paralogs are only available for a handful of plant *** we review our current knowledge on the GGPPS families of the model plant Arabidopsis thaliana and the crop species rice(0ryza sativa),pepper(Capsicum annuum)and tomato(Solanum lycopersicum).The results indicate that a major determinant of the biological role of particular GGPPS paralogs is the expression profile of the corresponding genes even though specific interactions with other proteins(including GGPP-consuming enzymes)might also contribute to *** some species,however,a single GGPPS isoforms appears to be responsible for the production of most if not all GGPP required for cell *** the mechanisms regulating GGPPS activity in particular cell compartments,tissues,organs and plant species will be very useful for future metabolic engineering approaches aimed to manipulate the accumulation of particular GGPP-derived products of interest without negatively impacting the levels of other isoprenoids required to sustain essential cell functions.

关键词： Geranylgeranyl diphosphate GGPP GGPP synthase Isoprenoid Isoprenyl transferase

A novel artificial bee colony-optimized visible oblique dipyramid greenness index for visionbased aquaponic lettuce biophysical signatures estimation

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Information Processing in Agriculture 2023年第3期10卷 312-333页

作者： Ronnie concepcion II Elmer Dadios Edwin Sybingco Argel Bandala Department of Manufacturing Engineering and Management De La Salle UniversityManila 1004Philippines Department of Electronics and Computer Engineering De La Salle UniversityManila 1004Philippines Center for Engineering and Sustainable Development Research De La Salle UniversityManila 1004Philippines

In response to the challenges in providing real-time extraction of crop biophysical signatures,computer vision in computational crop phenotyping highlights the opportunities of computational intelligence *** and angular brightness due to the presence of photosynthetic light unevenly illuminate crop *** this study,a novel vegetation index named artificial bee colony-optimized visible band oblique dipyramid greenness index(vODGIabc)was proposed to enhance vegetation pixels by correcting the saturation and brightness levels,and the ratio of visible RGB reflectance ***-grade smartphone was used to acquire indoor and outdoor aquaponic lettuce images daily for full 6-week crop life *** introduced saturation rectification coeffi-cient(X),value rectification coefficient(m),green–red wavelength adjustment factor(a),and green–blue wavelength adjustment factor(b)on the original triangular greenness index resulted in 3D canopy reflectance spectrum with two oblique tetrahedrons formed by connecting the vertices of visible RGB band reflectance and maximum wavelength point map to corresponding saturation and value of lettuce-captured *** neighborhood component analysis(NCA),minimum redundancy maximum relevance(MRMR),Pearson’s correlation coefficient(PCC),and analysis of variance(ANOVA)weighted most of the canopy area,energy,and *** linear relationships were exhibited by using vODGIabc in estimating lettuce crop fresh weight,height,number of spanning leaves,leaf area index,and growth stage with R2 values of 0.9368 for InceptionV3,0.9574 for ResNet101,0.9612 for ResNet101,0.9999 for Gaussian processing regression,and accuracy of 88.89%for ResNet101,*** low-cost approach on developing greenness index for biophysical signatures estimation proved to be more accurate than the previously established triangular greenness index(TGI)using RGB smartphone camera.

关键词： Lettuce Plant phenotype Precision farming Remote sensing Swarm intelligence Vegetation index

Structural effects on the magnetic hyperthermia properties of iron oxide nanoparticles

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Progress in Natural Science:Materials International 2016年第5期26卷 440-448页

作者： Eric C.Abenojar Sameera Wickramasinghe Jesbaniris Bas-concepcion Anna Cristina S.Samia Department of Chemistry Case Western Reserve University Chemistry Department University of Puerto Rico Rio Piedras Campus

Magnetic iron oxide nanoparticles(IONPs) are heavily explored as diagnostic and therapeutic agents due to their low cost, tunable properties, and biocompatibility. In particular, upon excitation with an alternating current(AC) magnetic field, the NPs generate localized heat that can be exploited for therapeutic hyperthermia treatment of diseased cells or pathogenic microbes. In this review, we focus on how structural changes and inter-particle interactions affect the heating efficiency of iron oxide-based magnetic NPs. Moreover, we present an overview of the different approaches to evaluate the heating performance of IONPs and introduce a new theranostic modality based on magnetic imaging guided–hyperthermia.

关键词： Iron oxide nanoparticles Magnetic hyperthermia Magneto-structural effects Magnetic dipolar interactions Magnetic imaging guided–hyperthermia

Aquaphotomics determination of nutrient biomarker for spectrophotometric parameterization of crop growth primary macronutrients using genetic programming

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Information Processing in Agriculture 2022年第4期9卷 497-513页

作者： Ronnie concepcion II Sandy Lauguico Jonnel Alejandrino Elmer Dadios Edwin Sybingco Argel Bandala Department of Manufacturing Engineering and Management De La Salle UniversityManila 1004Philippines Department of Electronics and Computer Engineering De La Salle UniversityManila 1004Philippines Center for Engineering and Sustainable Development Research De La Salle UniversityManila 1004Philippines

Water quality assessment is currently based on time-consuming and costly laboratory pro-cedures and numerous expensive physicochemical sensors *** response to the trend of device minimization and reduced outlays in sustainable aquaponic water monitoring,the integration of aquaphotomics and computational intelligence is presented in this *** study used the combination of temperature,pH,and electrical conductivity sensors in predicting crop growth primary macronutrient concentration(nitrate,phos-phate,and potassium(NPK)),thus,limiting the number of deployed sensors.A total of 220 water samples collected from an outdoor artificial aquaponic pond were temperature perturbed from 16 to 36℃ with 2℃ increments to mimic ambient range,which varies water compositional *** was applied on ultraviolet,visible light,and near-infrared spectral regions,100 to 1000 nm,to determine NPK ***-pal component analysis emphasized nutrient dynamics through selecting the highly corre-lated water absorption bands resulting in 250 nm,840 nm,and 765 nm for nitrate,phosphate,and potassium *** activated water bands were used as wave-length protocols to spectrophotometrically measure macronutrient ***-iments have shown that multigene symbolic regression genetic programming(MSRGP)obtained the optimal performance in parameterizing and predicting nitrate,phosphate,and potassium concentrations based on water physical properties with an accuracy of 87.63%,88.73%,and 99.91%,*** results have shown the established 4-dimensional nutrient dynamics map reveals that temperature significantly strengthens nitrate and potassium above 30℃ and phosphate below 25℃ with pH and electrical con-ductivity ranging between 7 and 8 and 0.1 to 0.2 mS cm^(-1) *** novel approach of developing a physicochemical estimation model predicted macronutrient concentra-tions in real-time using physical limnological sensors with a

关键词： Aquaphotomics Genetic programming Plant nutrients Physicochemical composition Spectrophotometry Water quality monitoring

Direct conversion of human fibroblasts into retinal pigment epithelium-like cells by defined factors

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Protein & Cell 2014年第1期5卷 48-58页

作者： Kejing Zhang Guang-Hui Liu Fei yi Nuria Montserrat Tomoaki Hishida concepcion Rodriguez Esteban Juan Carlos Izpisua Belmonte] Gene Expression Laboratory Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Department of Molecular and Cellular Physiology Stanford University School of Medicine 265 Campus Drive StanfordCA 94305 USA Center for Regenerative Medicine in Barcelona Dr. Aiguader 88 08003 Barcelona Spain Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine (CIBER-BBN) BarcelonaSpain

The generation of functional retinal pigment epithelium （RPE） is of great therapeutic interest to the field of regenerative medicine and may provide possible cures for retinal degenerative diseases, including age-related macular degeneration （AMD）. Although RPE cells can be produced from either embryonic stem cells or induced pluripotent stem cells, direct cell reprogramming driven by lineage-determining transcription factors provides an immediate route to their generation. By monitoring a human RPE specific Bestl：：GFP reporter, we report the conversion of human fibroblasts into RPE lineage using defined sets of transcription factors. We found that Bestl：：GFP positive cells formed colonies and exhibited morphological and molecular features of early stage RPE cells. Moreover, they were able to obtain pigmen- tation upon activation of Retinoic acid （RA） and Sonic Hedgehog （SHH） signaling pathways. Our study not only established an ideal platform to investigate the tran- scriptional network regulating the RPE cell fate deter- mination, but also provided an alternative strategy to generate functional RPE cells that complement the useof pluripotent stem cells for disease modeling, drug screening, and cell therapy of retinal degeneration.

关键词： retinal pigment epithelium fibroblasts,direct conversion

The Body in Old Age and Dependency： A Territory of Threat and Uncertainty

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Sociology Study 2015年第7期5卷 562-576页

作者： maria concepcion Arroyo Universidad Juarez del Estado de Durango (JuarezUniversity of Durango) Mexico

This paper explores the image of the body in physically dependent elderly men and women and the way in which this image reconfigures their identity creating new meanings. In old age, the body becomes related with illness, with disablement and with its own material finiteness. Representations of the body are thus constructed around pain, deficiency, and fragility. The research was carried out from a qualitative perspective, performing in-depth interviews, with participant observation and a subject ID card as data collecting techniques. The identity of the interviewees who consented to be recorded was protected. The data were analyzed constructing concepts and theoretical and empirical categories with the support of the Etnograph V.5 software for qualitative data. The findings reflect discourses on the body that turn on its deterioration and limitations that prevent old people from functioning adequately in life. Metaphors were identified telling of a sense of＂deteriorated identity＂; most participants saw themselves as ＂a burden＂. They also showed symptoms of annoyance and shame regarding their sick or fragile bodies, as well as a constant memory of the healthy or ＂ideal＂ body of the past, which is hegemonic in our culture.

关键词： Body old age dependency identity

The Pact for Mexico as a Mechanism of Governance and Legitimacy

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International Relations and Diplomacy 2014年第11期2卷 736-747页

作者： Luis Enrique concepcion Montiel Autonomous University of Baja California USA

Govemability is the quality of a political community, according to which its institutions of Government act effectively within its space, in a way considered legitimate by the citizens, thus allowing the flee exercise of the political will of the Executive branch through civic obedience of the people. Recently in Mexico, it has referred repeatedly to the precariousness of the governability by the incidence of insecurity, unemployment and extreme poverty. The Pact for Mexico--attempts to answer to these problems--is a political agreement between the President of the Republic and the three main parties--Institutional Revolutionary Party （PRI）, the National Action Party （PAN）, and the Party of the Democratic Revolution （PRD）--for big actions and specific reforms which, according to the signatories, will see Mexico toward a more prosperous future, leading the democratic transition and drive economic growth that generates quality jobs and allow to reduce poverty and social inequality. In this sense, the main axes of the Pact include those related to economic growth, competitiveness, employment, security and agreements for governability. The purpose of this paper is to analyze the Pact for Mexico from the perspective of govemability, since its dimensions of legitimacy and effectiveness. It will analyze the main reforms and political agreements, the role of Congress and the main actors; as well as the arguments of those who defend the Pact stating that it is a mechanism of governability and those who reject it that it overshadows the work of the legislature.

关键词： govemability governance legitimacy pact