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水禽4种常见致病菌多重PCR方法的建立及应用

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黑龙江畜牧兽医 2024年第5期 66-72页

作者：蒲龄张亚楠彭珊杨茂生徐景峨贵州省农业科学院畜牧兽医研究所贵阳550005 广东省现代农业装备研究所广州510000 佛山科学技术学院广东佛山528231

为建立一种能同时检测大肠杆菌、鸭疫里默氏杆菌、多杀性巴氏杆菌、金黄色葡萄球菌的多重PCR方法,试验根据大肠杆菌phoA基因、鸭疫里默氏杆菌ompA基因、多杀性巴氏杆菌kmt 1基因、金黄色葡萄球菌FemB基因的保守区域设计4对特异性引物,... 详细信息

为建立一种能同时检测大肠杆菌、鸭疫里默氏杆菌、多杀性巴氏杆菌、金黄色葡萄球菌的多重PCR方法,试验根据大肠杆菌phoA基因、鸭疫里默氏杆菌ompA基因、多杀性巴氏杆菌kmt 1基因、金黄色葡萄球菌FemB基因的保守区域设计4对特异性引物,通过优化退火温度和引物浓度建立多重PCR方法,检验该方法的特异性、敏感性性、重复性,应用该方法检测145份病死水禽临床样品,并与传统细菌分离鉴定方法进行比较。结果表明:优化后的最佳退火温度为52℃,引物浓度为10μmol/L。建立的多重PCR方法能特异性地扩增出靶基因,沙门氏菌、链球菌、产气荚膜梭菌等临床常见菌均未扩增出条带,对大肠杆菌、鸭疫里默氏杆菌、多杀性巴氏杆菌、金黄色葡萄球菌4种细菌的检出下限是0.4 pg/mL,单一PCR扩增ompA、kmt 1、phoA和FemB基因的检出下限分别为4×10^(-3),0.4,4×10^(-3),4×10^(-6)pg/mL。多重PCR方法对临床样品的检测结果与传统细菌分离鉴定方法的符合率均在90%以上。说明试验建立的多重PCR方法具有结果稳定、重复性高的特点,适合水禽病原微生物流行病学调查。

关键词：多重PCR方法大肠杆菌鸭疫里默氏杆菌多杀性巴氏杆菌金黄色葡萄球菌

Oleic acid promotes atherosclerosis via multiple pathophysiological mechanisms:a narrative review

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Journal of Bio-X Research 2022年第2期5卷 64-66页

作者： Ting Chu maosheng yang Department of Nursing School of NursingZhejiang Chinese Medical University HangzhouZhejiang Province Laboratory of Disorders Genes and Department of Pharmacology Jishou University School of PharmacyJishouHunan ProvinceChina

An increasing body of evidence supports the hypothesis that atherosclerosis is caused by multiple factors and mechanisms.A better understanding of the contribution of hypertriglyceridemia or oleic acid to the pathogenesis of atherosclerosis would significantly increase our ability to successfully treat these *** propose that oleic acid promotes atherosclerosis via multiple pathophysiological *** we suggest that(1)atherosclerosis may develop through multiple pathophysiological mechanisms and that this may be a common feature of human diseases;(2)diseases may be the consequence of natural selection or the interaction of internal and external factors;(3)diseases should be recognized with“biological diversity,”and that this concept should be introduced into medical education,clinical treatment,drug research and development,and medical *** work reported here will benefit a new generation of medical trainees and promote the development of the relevant medical fields.

关键词： atherosclerosis biological diversity cardiovascular disease hypertriglyceridemia oleic acid

High-sensitivity modulation of electromagnetically induced transparency analog in a THz asymmetric metasurface integrating perovskite and graphene

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Photonics Research 2022年第10期10卷 2317-2327页

作者： TONGLING WANG TENGTENG LI HAIYUN YAO YUYING LU XIN YAN MAOYONG CAO LANJU LIANG maosheng yang JIANQUAN YAO School of Information and Automation Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353China State Key Laboratory of Dynamic Measurement Technology North University of ChinaTaiyuan 030051China School of Opto-electronic Engineering Zaozhuang UniversityZaozhuang 277160China Precision Optical Manufacturing and Testing Centre Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China School of Electrical and Optoelectronic Engineering West Anhui UniversityLu’an 237000China

Active control of the electromagnetically induced transparency(EIT)analog is desirable in photonics ***,we theoretically and experimentally proposed a novel terahertz(THz)asymmetric metasurface structure that can possess high-sensitivity modulation under extremely low power density by integrating perovskite or *** the novel metasurface structure with the perovskite coating,the maximum amplitude modulation depth(AMD)of this perovskite-based device reached 490.53%at a low power density of 12.8037 mW/cm^(2).In addition,after the novel THz metasurface structure was combined with graphene,this graphene-based device also achieved high AMD with the maximum AMD being 180.56%at 16.312 mW/cm^(2),and its transmission amplitude could be electrically driven at a low bias *** physical origin of this modulation was explained using a two-oscillator EIT *** work provides a promising platform for developing high-sensitivity THz sensors,light modulators,and switches.

关键词： perovskite integrating transparency

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牛羊有机磷农药中毒的救治

现代畜牧科技 2024年第6期 103-105页

作者：陈川燕赵嫣瑜杨茂生李红芬杨希董仲生云南省大理市祥云县下庄镇农村综合服务中心云南祥云672105 云南省大理市畜牧兽医中心云南大理671014 云南省腾冲市畜牧工作站云南腾冲679100 云南省祥云县王家庄街道农业农村综合服务中心云南祥云655102 云南农业职业技术学院云南昆明650212

家畜有机磷农药中毒曾为兽医临床常见多发病之一,造成较大的损失。至今,农作物、蔬菜及果树仍普遍使用有机磷农药防控病虫害,仍存在直接将有机磷农药涂擦在动物体表,治疗皮肤寄生虫病而致中毒的现象。用硫酸阿托品和碘解磷定救治牛羊有... 详细信息

家畜有机磷农药中毒曾为兽医临床常见多发病之一,造成较大的损失。至今,农作物、蔬菜及果树仍普遍使用有机磷农药防控病虫害,仍存在直接将有机磷农药涂擦在动物体表,治疗皮肤寄生虫病而致中毒的现象。用硫酸阿托品和碘解磷定救治牛羊有机磷农药中毒,有立竿见影的效果,只要救治及时,反复、持续用药足够长的时间,危症病例也几乎能治愈。2013年以来,在云南省祥云县下庄镇救治牛羊有机磷中毒67次,共207头牛、212只羊,总治愈率99.3%。救治过程中发生死亡的病例,主要是救治不及时、或硫酸阿托品重复注射不及时,偶尔因阿托品、碘解磷定使用过量造成严重中毒。

关键词：牛羊有机磷农药中毒救治

A review of structural modification and biological activities of oleanolic acid

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Chinese Journal of Natural Medicines 2024年第1期22卷 15-30页

作者： yang Huali DENG Minghui JIA Hongwei ZHANG Kaicheng LIU yang CHENG maosheng XIAO Wei State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process Jiangsu Kanion Pharmaceutical Co.Ltd.Lianyungang 222001China Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of Education School of Pharmaceutical EngineeringShenyang Pharmaceutical UniversityShenyang 110016China

Oleanolic acid(OA),a pentacyclic triterpenoid,exhibits a broad spectrum of biological activities,including antitumor,antiviral,antibacterial,anti-inflammatory,hepatoprotective,hypoglycemic,and hypolipidemic *** its initial isolation and identification,numerous studies have reported on the structural modifications and pharmacological activities of OA and its *** this,there has been a dearth of comprehensive reviews in the past two decades,leading to challenges in subsequent research on *** on the main biological activities of OA,this paper comprehensively summarized the modification strategies and structureactivity relationships(SARs)of OA and its derivatives to provide valuable reference for future investigations into OA.

关键词： Oleanolic acid Derivatives Pentacyclic triterpenoid Structural modification Biological activities

Hybrid metasurface using graphene/graphitic carbon nitride heterojunctions for ultrasensitive terahertz biosensors with tunable energy band structure

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Photonics Research 2023年第5期11卷 858-868页

作者： HAIYUN YAO ZHAOQING SUN LANJU LIANG XIN YAN YARUWANG maosheng yang XIAOFEI HU ZIQUN WANG ZHENHUA LI MENG WANG CHUANXIN HUANG QILI yang ZHONGJUN TIAN JIANQUAN YAO School of Opto-electronic Engineering Zaozhuang UniversityZaozhuang 277160China Faculty of Materials and Manufacturing Beijing University of TechnologyBeijing 100124China School of Information Science and Engineering Zaozhuang UniversityZaozhuang 277160China School of Electrical and Optoelectronic Engineering West Anhui UniversityLu’an 237000China College of Precision Instruments and Opto-electronics Engineering Tianjin UniversityTianjin 300072China

Integrating novel materials is critical for the ultrasensitive,multi-dimensional detection of biomolecules in the terahertz(THz)*** studies on THz biosensors have used semiconductive active layers with tunable energy band *** this study,we demonstrate three THz biosensors for detecting casein molecules based on the hybridization of the metasurface with graphitic carbon nitride,graphene,and *** achieved lowconcentration detection of casein molecules with a 3.54 ng/m L limit and multi-dimensional sensing by observing three degrees of variations(frequency shift,transmission difference,and phase difference).The favorable effect of casein on the conductivity of the semiconductive active layer can be used to explain the internal sensing *** incorporation of protein molecules changes the carrier concentration on the surface of the semiconductor active layer via the electrostatic doping effect as the concentration of positively charged casein grows,which alters the energy band structure and the conductivity of the active *** measured results indicate that any casein concentration can be distinguished directly by observing variations in resonance frequency,transmission value,and phase *** the heterojunction,the biosensor showed the highest response to the protein among the three *** Silvaco Atlas package was used to simulate the three samples'energy band structure and carrier transport to demonstrate the benefits of the heterojunction for the *** simulation results validated our proposed theoretical mechanism *** proposed biosensors could provide a novel approach for THz metasurface-based ultrasensitive biosensing technologies.

关键词： heterojunction tunable surface

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阿托品治疗肉牛有机磷农药中毒的试验

现代畜牧科技 2024年第8期 107-109页

作者：李红芬赵嫣瑜杨茂生何土开杨希陈川燕董仲生云南省祥云县王家庄街道农业农村综合服务中心云南祥云655102 云南省大理市畜牧兽医中心云南大理671014 云南省腾冲市畜牧工作站云南腾冲679100 云南省大理市祥云县下庄镇畜牧兽医站云南祥云672105 云南农业职业技术学院云南昆明650212

为简化肉牛有机磷农药中毒的救治方法,2013—2022年,在王家庄街道救治肉牛有机磷中毒192例,其中轻症组73例、重症组67例、危症组52例,都只用硫酸阿托品,未用碘解磷啶;同期,在马过河镇应用硫酸阿托品和碘解磷啶救治肉牛67例作对照,其中... 详细信息

为简化肉牛有机磷农药中毒的救治方法,2013—2022年,在王家庄街道救治肉牛有机磷中毒192例,其中轻症组73例、重症组67例、危症组52例,都只用硫酸阿托品,未用碘解磷啶;同期,在马过河镇应用硫酸阿托品和碘解磷啶救治肉牛67例作对照,其中轻症组28例、重症组22例、危症组17例。结果:6个组中,只有王家庄街道危症组的治愈率不足100%(98.1%),差异不显著(P>0.05);阿托品平均注射次数、平均用量及平均疗程,轻症组差异显著(P<0.05),重症和危症组差异均极显著(P<0.01)。肉牛有机磷农药中毒的解救,轻症可以不用碘解磷啶;只要重复注射阿托品的时机及剂量掌握得当,重症和危症均可不用碘解磷啶。

关键词：肉牛有机磷农药中毒阿托品治疗试验

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瘤胃投碘解磷定救治牛有机磷农药中毒的试验

现代畜牧科技 2024年第7期 1-4页

作者：杨茂生李红芬何土开赵嫣瑜杨希陈川燕董仲生云南省腾冲市畜牧工作站云南腾冲679100 云南省曲靖市马龙区王家庄街道农业农村综合服务中心云南马龙655102 云南省大理市畜牧兽医中心云南大理671014 云南省祥云县下庄镇农业综合服务中心云南祥云672105 云南农业职业技术学院云南昆明650212

为克服牛有机磷农药中毒救治过程中,部分患牛瘤胃冲洗困难造成硫酸阿托品用量大、疗程长的问题。将腾冲县12个乡镇339例牛有机磷农药中毒病例,分为对照组62例,按常规注射硫酸阿托品、碘解磷定和对症治疗;试验组277例,在对照组的基础上,... 详细信息

为克服牛有机磷农药中毒救治过程中,部分患牛瘤胃冲洗困难造成硫酸阿托品用量大、疗程长的问题。将腾冲县12个乡镇339例牛有机磷农药中毒病例,分为对照组62例,按常规注射硫酸阿托品、碘解磷定和对症治疗;试验组277例,在对照组的基础上,用胃管投碘解磷定。结果显示,2组轻症的硫酸阿托品平均注射次数、总平均注射量差异显著(P0.05)。研究认为,及早给瘤胃投碘解磷定可大幅度提高牛有机磷农药中毒的抢救效率。

关键词：有机磷农药中毒瘤胃碘解磷定救治试验

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牛康复后再次有机磷农药中毒的防控试验

现代畜牧科技 2024年第4期 19-21页

作者：李红芬杨茂生何土开陈川燕赵嫣瑜杨希董仲生云南省王家庄街道农业农村综合服务中心云南马龙655102 云南省腾冲市畜牧工作站云南腾冲679100 云南省大理市畜牧兽医中心云南大理671014 云南省下庄镇畜牧兽医站云南祥云672105 云南农业职业技术学院云南昆明650212

为防控牛有机磷农药中毒治愈后再次发生慢性蓄积性中毒的现象,2012—2022年,对王家庄街道221例有机磷农药中毒治愈的牛,每隔7~10 d使用1次预防量的碘解磷定脱毒,至当地频繁使用有机磷农药的季节结束后,再继续使用1个月为止。结果显示,22... 详细信息

为防控牛有机磷农药中毒治愈后再次发生慢性蓄积性中毒的现象,2012—2022年,对王家庄街道221例有机磷农药中毒治愈的牛,每隔7~10 d使用1次预防量的碘解磷定脱毒,至当地频繁使用有机磷农药的季节结束后,再继续使用1个月为止。结果显示,221例治愈的牛,在3个月内无重复中毒的现象;与2005—2011年成功救治王家庄街道172个病例,重复中毒31例,复发率18%;及同期内,临近的马河镇何家村治愈32个病例,重复中毒7例,复发率21.9%。在高频率使用有机磷农药,牛有机磷农药中毒病例较多的地区,治愈后间歇使用预防量的碘解磷定,消除体内慢性蓄积的有机磷农药,可有效避免再次中毒的发生。

关键词：牛有机磷农药中毒预防控制试验