检索结果-南通市图书馆

World Journal of Pediatrics 2015年第4期11卷 380-385页

作者： Shu Teng Yi wei Shi-Yong Zhao Xian-Yao Lin Qi-min Shao Juan Wang Department of Pediatric Infection Hangzhou Children's HospitalHangzhouChina

Background:Hand-foot-and-mouth disease(HFMD)is a common pediatric infectious disease caused by a variety of intestinal *** 71(EV71)is the primary pathogen that might cause severe symptoms and even death in children with *** study aimed to investigate the intestinal detoxification time of HFMD children with EV71 infection and its related ***:Sixty-five HFMD children with EV71 infection were followed *** stool samples were collected once every 4 to 7 *** nucleic acids were detected byfl uorescent polymerase chain reaction until the results became *** positive rates of viral nucleic acids were analyzed by the Kaplan-Meier *** Log-rank test and Cox-Mantel test were used to analyze factors affecting the HFMD children with EV71 ***:On the 2nd,4th,6th and 10th week,the positive rates of viral nucleic acids in stool samples of the 65 children were 94.6%,48.1%,17.2%and 0,*** analysis showed that the intestinal detoxification time of the children were related to gender,pre-admission disease course,severity of disease,and use of steroids or gamma globulin(P<0.05).Multivariate analysis showed that the severity of disease was an independent factor affecting the intestinal detoxification time(P<0.05),with a relative risk of ***:The longest intestinal detoxification time of HFMD children with EV71 infection was 10 *** severity of disease was an important factor affecting the intestinal detoxification time of HFMD children with EV71 *** HFMD children with EV71 infection had a longer intestinal detoxification time.

关键词： detoxification time enterovirus 71 hand-foot-and-mouth disease relative factor

Circadian disruption and sleep disorders in neurodegeneration

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Translational Neurodegeneration 2023年第1期12卷 766-790页

作者： Yun Shen Qian-kun Lv wei-ye Xie Si-yi Gong Sheng Zhuang Jun-yi Liu Cheng-jie mao Chun-feng Liu Department of Neurology and Clinical Research Center of Neurological Disease The Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience Soochow UniversitySuzhou 215123China Department of Psychiatry Faculty of MedicineThe Chinese University of Hong KongShatinN.T.Hong Kong SARChina Department of Neurology Dushu Lake Hospital Affiliated to Soochow UniversitySuzhou 215125China

Disruptions of circadian rhythms and sleep cycles are common among neurodegenerative diseases and can occur at multiple *** evidence reveals a bidirectional relationship between disruptions of circadian rhythms and sleep cycles and neurodegenerative *** disruption and sleep disorders aggravate neurodegeneration and neurodegenerative diseases can in turn disrupt circadian rhythms and ***,circadian disruption and various sleep disorders can increase the risk of neurodegenerative ***,harnessing the circadian biology findings from preclinical and translational research in neurodegenerative diseases is of importance for reducing risk of neurodegeneration and improving symptoms and quality of life of individuals with neurodegenerative disorders via approaches that normalize circadian in the context of precision *** this review,we discuss the implications of circadian disruption and sleep disorders in neurodegenerative diseases by summarizing evidence from both human and animal studies,focusing on the bidirectional links of sleep and circadian rhythms with prevalent forms of *** findings provide valuable insights into the pathogenesis of neurodegenerative diseases and suggest a promising role of circadian-based interventions.

关键词： Circadian disruption Sleep disorder Parkinson’s disease Alzheimer’s disease

Reaction Kinetic Equation for Char Combustion of Underground Coal Gasification

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of China University of mining and Technology 2006年第2期16卷 141-146页

作者： YU Hong-guan YANG Lan-he FENG wei-min LIU Shu-qin SONG Zhen-qi School of Chemical and Environmental Engineering Shandong University of Science and Technology Qingdao Shandong 266510 China School of mineral Resource and Geoscience China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China Engineering Department Beizao Coal Mine Group Companies of Longkou Mining Industry Longkou Shangdong 265700 China School of Chemical and Environmental Engineering China University of Mining ＆ Technology Beijing 100083 China School of mineral Resource and Environmental Engineering Shandong University of Science and Technology Qingdao Shandong 266510 China

Based on the quasi-steady-state approximation, the dynamic equation of char combustion in the oxidation zone of underground coal gasification (UCG) was derived. The parameters of the dynamic equation were determined at 900℃ using a thermo-gravimetric (TG) analyzer connected to a flue gas analyzer and this equation. The equation was simplified for specific coals, including high ash content, low ash content, and low ash fusibility ones. The results show that 1) the apparent reaction rate constant increases with an increase in volatile matter value as dry ash-free basis,2) the effective coefficient of diffusion decreases with an increase in ash as dry basis, and 3) the mass transfer coefficient is independent of coal quality on the whole. The apparent reaction rate constant, mass-transfer coefficient and effective coefficient of diffusion of six char samples range from 7.51×104 m/s to 8.98×104 m/s, 3.05×106 m/s to 3.23×106 m/s and 5.36×106 m2/s to 8.23×106 m2/s at 900℃, respectively.

关键词： underground coal gasification char combustion kinetic equation thermo-gravimetric

新孢子虫硫氧还蛋白的生物信息学分析及其原核表达的鉴定

在线全文

学校读者我要写书评

暂无评论

中国预防兽医学报 2024年第7期46卷 691-699页

作者：齐闻新张旻李晨周思含徐啊慧钱伟锋胡苏辉王天奇位治国洪炀闫文朝河南科技大学动物科技学院河南洛阳471023 中国疾病预防控制中心寄生虫病预防控制所国家卫生健康委员会寄生虫病原与媒介生物学重点实验室上海200241

为研究新孢子虫硫氧还蛋白(Trx)的生物学特性,本研究利用PCR扩增犬新孢子虫Trx(NcTrx)基因的编码区序列,构建pET-32a(+)-NcTrx原核表达载体,经双酶切与测序鉴定正确后,利用系列生物信息学软件分析NcTrx基因及其编码氨基酸序列的生物信... 详细信息

为研究新孢子虫硫氧还蛋白(Trx)的生物学特性,本研究利用PCR扩增犬新孢子虫Trx(NcTrx)基因的编码区序列,构建pET-32a(+)-NcTrx原核表达载体,经双酶切与测序鉴定正确后,利用系列生物信息学软件分析NcTrx基因及其编码氨基酸序列的生物信息学特征,并对其蛋白互作网络中排名前100的蛋白进行GO功能和KEGG信号通路的富集分析。重组质粒中NcTrx基因的测序结果显示,NcTrx基因片段为1 287 bp,编码428个氨基酸;该蛋白含1个信号肽,1个跨膜区,主要位于内质网上;NcTrx蛋白的二、三级结构主要由α-螺旋、无规则卷曲组成,有12个B细胞抗原表位;该蛋白属于硫氧还蛋白家族,存在典型的氧化还原基序“-CXXC-”,有38个磷酸化位点、10个O-糖基化位点;与NcTrx蛋白互作最强的为酪氨酸激酶样蛋白,二者通过氢键和静电作用实现相互对接;GO功能及KEGG信号通路的显著性富集分析结果显示,NcTrx与其互作的蛋白主要参与蛋白质转运、蛋白质二硫键异构酶活性等生物学过程,与内质网蛋白质加工、硫代谢等信号通路密切相关。将pET-32a(+)-NcTrx转化BL21(DE3)感受态细胞,并经IPTG诱导表达后采用镍亲和层析法纯化重组NcTrx蛋白(rNcTrx),经SDS-PAGE检测该蛋白的表达及纯化效果,采用Bradford法测定纯化蛋白的浓度。采用western blot鉴定rNcTrx的反应原性。SDS-PAGE结果显示,rNcTrx分子量约66 ku,主要以包涵体的形式表达,且纯化效果较好,浓度为0.8 mg/mL。Western blot检测结果显示,纯化的rNcTrx能够与鼠His MAb、新孢子虫感染的小鼠血清发生特异性反应,在66 ku处出现特异性条带。本研究首次经PCR扩增NcTrx基因,对NcTrx基因及其蛋白进行了生物信息学分析,并经原核系统表达及鉴定了NcTrx蛋白,为后续深入探究该蛋白的生物学功能提供参考依据。

关键词：新孢子虫硫氧还蛋白生物信息学原核表达

基于改进随机森林算法的汽轮机振动故障诊断研究

在线全文

学校读者我要写书评

暂无评论

浙江电力 2024年第9期43卷 107-116页

作者：李蔚吴懿范毛静宇常增军李仲博王方舟浙江大学能源工程学院杭州310027 北京热力集团有限责任公司北京100028 北京京能能源技术研究有限责任公司北京100089

随机森林算法具有抗噪声和计算能力强的优点,被广泛应用于旋转机械的振动故障诊断中,但在工业场景中存在样本较少、无法引入先验知识、准确度较低等问题。对此,基于层次分析思想,利用信息熵引入先验知识优化决策树,提出了基于IRF(改进... 详细信息

随机森林算法具有抗噪声和计算能力强的优点,被广泛应用于旋转机械的振动故障诊断中,但在工业场景中存在样本较少、无法引入先验知识、准确度较低等问题。对此,基于层次分析思想,利用信息熵引入先验知识优化决策树,提出了基于IRF(改进随机森林算法)的汽轮机振动故障诊断方法。为验证所提方法的有效性和可靠性,采用某百万火电机组数据中心的真实运行数据集进行评估。计算结果表明,相较于经典随机森林算法,IRF能够在降低33%决策树数目的情况下具有更高的精确度和低漏报率,同时运行时间缩短至经典随机森林算法的11.4%,在火电机组实时精确振动故障诊断方面有较高的实用价值。

关键词：汽轮机振动故障诊断改进随机森林算法层次分析信息熵

Influence of various stressors on the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis

在线全文

学校读者我要写书评

暂无评论

Insect Science 2017年第3期24卷 418-430页

作者： Yi-Fei Xie Jin-Zhi Niu Xuan-Zhao Jiang Wen-Jia Yang Guang-mao Shen Dong wei Guy Smagghe Jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing China Department of Crop Protection Faculty of Bioscience Engineering Ghent University Ghent Belgium Hunan Academy of Forestry Changsha China

RNA interference （RNAi）-based technology has emerged as a potential tool for controlling insect pests, however, previous studies found that the efficiency of RNAi in Bactrocera dorsalis was variable. In nature, insects often meet various challenges, such as pathogen infections, extreme temperatures, lack of nutrition and heavy metals. To better understand the association of the stressors with efficiency of RNAi, in the current study we tested the expression of three core genes, dicer2 （Bddcr2）, r2d2 （Bdr2d2） and argonaute2 （Bdago2）, of the small interfering RNA （siRNA） pathway of B. dorsalis upon various stressors. Our results showed that all three genes were upregulated by the infection of invertebrate iridescent virus 6, which suggested a function of the siRNA pathway against viral infection. The loading of FeC13 could also increase the expression of Bddcr2. The treatments ofEscherichia coli, extremely high （40~C） and low （0~C） temperatures, as well as starvation, could negatively influence the expression of Bddcr2 and/or Bdago2. In total, our results showed that various stressors could influence the expression of core components ofB. dorsalis siRNA pathway. This highlights further speculation on the RNAi efficiency upon these stressors. Considering the complexity and variation of RNAi efficiency in different conditions, these results provide initial aspects in possible environmental stressors to influence the activity of the siRNA pathway, but the real impact of RNAi efficiency posed by these stressors requires further studies.

关键词： argonaute Bactrocera dorsalis dicer RNA interference R2D2 stressor

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

低开销的匿名通信群组威胁人物挖掘方法

四川大学学报（自然科学版） 2024年第4期61卷 37-46页

作者：霍艺璇赵佳鹏时金桥齐敏孙岩炜王学宾杨燕燕北京邮电大学网络空间安全学院北京100876 中国科学院信息工程研究所北京100093 中国人民公安大学信息网络安全学院北京100038

深暗网因其强隐匿性、接入简便性和交易便捷性,滋生了大量非法活动,如推广网络博彩、贩卖毒品等.随着网络社交方式的更新,加密即时通信工具Telegram中的群组成为不法分子推广黑灰产、买卖资源和工具的聚集地,大量不法分子利用Telegram... 详细信息

深暗网因其强隐匿性、接入简便性和交易便捷性,滋生了大量非法活动,如推广网络博彩、贩卖毒品等.随着网络社交方式的更新,加密即时通信工具Telegram中的群组成为不法分子推广黑灰产、买卖资源和工具的聚集地,大量不法分子利用Telegram的匿名功能在对内容无限制、消息短、文字难理解的群组中推动业务而逃避监管,严重威胁国家社会稳定和网络安全.如果能够基于对群组中大量低信息量内容的分析,挖掘大批量潜在威胁人物,将为监管、治理和打击部门提供更多有价值的线索.本文提出一种低开销的匿名通信群组威胁人物挖掘方法,通过调整文本中网络公害流行术语的重要程度优化内容分析质量,融合大语言模型的强大知识储备和生成能力,对群组内容进行无监督的高质量动态时序主题提取与可视化统计分析.实验结果表明,与传统分类做法相比,本文方法大大降低了人工标注成本,提升了威胁人物挖掘的数量和质量,加深了对网络公害生态的理解,具有现实意义.

关键词：网络公害文本挖掘 Telegram群组主题建模

不同饲粮粗蛋白质水平对槟榔江水牛泌乳性能的影响

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

动物营养学报 2024年第4期36卷 2457-2474页

作者：李安润陈艳美文泽芳张丽娟姜丰黄琴张瑞云史建伟金贞张敏余四娜毛华明李清云南农业大学动物科学技术学院云南省动物营养与饲料重点实验室昆明650201 云南省草地动物科学研究院昆明650212 陇川县科学技术协会德宏678700 个旧市畜牧技术推广站个旧661000 福贡县农业农村局怒江673400

本试验旨在探究不同饲粮粗蛋白质水平对槟榔江水牛泌乳性能的影响。试验采用单因素完全随机区组设计,将21头健康、年龄[(6.33±2.75)岁]和胎次[(3.14±1.56)胎]相近,且上胎产奶量相近的槟榔江水牛随机分为3个组(每组7头),分别饲喂低粗... 详细信息

本试验旨在探究不同饲粮粗蛋白质水平对槟榔江水牛泌乳性能的影响。试验采用单因素完全随机区组设计,将21头健康、年龄[(6.33±2.75)岁]和胎次[(3.14±1.56)胎]相近,且上胎产奶量相近的槟榔江水牛随机分为3个组(每组7头),分别饲喂低粗蛋白质水平(9.5%)、中粗蛋白质水平(12.5%)和高粗蛋白质水平(14.8%)的全混合日粮。预试期7 d,正试期273 d。结果表明:随着饲粮粗蛋白质水平的升高,槟榔江水牛泌乳性能相关指标均有一定变化,其中14.8%粗蛋白质组泌乳期日均产奶量、平均产奶量以及乳蛋白、乳非脂固形物和乳尿素氮含量均显著高于9.5%粗蛋白质组(P0.05);随着泌乳时间的增加,12.5%和14.8%粗蛋白质组乳脂含量基本都呈现为先降低后升高的变化趋势,9.5%粗蛋白质变化规律不明显,但3组泌乳末期乳脂含量都比泌乳初期高,且12.5%粗蛋白质组乳脂含量最高,为7.84%;3组平均泌乳天数、乳脂蛋比以及乳脂、乳糖和乳总固形物含量差异均不显著(P>0.05),且乳脂蛋比、乳糖和乳总固形物含量变化不大;12.5%粗蛋白质组乳体细胞数显著高于9.5%和14.8%粗蛋白质组(P<0.05)。由此可知,饲粮粗蛋白质水平从9.5%提高至14.8%可显著提高槟榔江水牛产奶量以及乳蛋白、乳非脂固形物和乳尿素氮含量,但对泌乳天数和乳脂、乳糖、乳总固形物含量以及乳脂蛋比无显著影响。

关键词：泌乳性能槟榔江水牛粗蛋白质水平

The role of targeting protein for Xklp2 in tumorigenesis of hepatocellular carcinoma

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2023年第4期22卷 418-422页

作者： Ting Li Lu-Jian Zhu An-min Huang Yi-Feng wei Jun Xu Ye-Jin Xu Department of Infectious Diseases Jinhua Municipal Central HospitalJinhua 321000China School of the First Clinical Medical Sciences(School of Information and Engineering) Wenzhou Medical UniversityWenzhou 325035China

To the Editor:Hepatocellular carcinoma(HCC)is a prevalent form of gastrointestinal *** current combination of immunotherapy and other treatments improves overall survival compared to conventional therapies,but the immunosuppressive microenvironment of HCC is a significant barrier to the efficacy of immunothera-peutic drugs[1].Tumor immune escape is promoted by interactions among immunosuppressive cells,including tumor-associated macrophages,marrow-derived suppressor cells,tumor-associated neutrophils,cancer-associated fibroblasts,and tumor-infiltrating Tregs[2].

关键词： drugs suppressor hepatocellular

Glycerol aerobic oxidation to glyceric acid over Pt/hydrotalcite catalysts at room temperature

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2019年第23期64卷 1764-1772页

作者： Junbo Zhang Xiaolin Li ming Xu Yusen Yang Yinwen Li Ning Liu Xiaoyu Meng Lifang Chen Shuxian Shi min wei State Key Laboratory of Chemical Resource Engineering Beijing Advanced Innovation Center for Soft Matter Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China College of Chemistry and Molecular Engineering and College of Engineering BIC-ESATPeking UniversityBeijing 100871China

Glycerol(GLY) aerobic oxidation in an aqueous solution is one of the most prospective pathways in biomass transformation, where the supported catalysts based on noble metals(mainly Au, Pd, Pt) are most commonly employed. Herein, Pt nanoparticles supported on rehydrated MgxAl1-hydrotalcite(denoted as re-MgxAl1-LDH-Pt) were prepared via impregnation-reduction method followed by an in situ rehydration process, which showed high activity and selectivity towards GLY oxidation to produce glyceric acid(GLYA) at room temperature. The metal-support interfacial structure and catalyst basicity were modulated by changing the Mg/Al molar ratio of the hydrotalcite precursor, and the optimal performance was achieved on re-Mg6Al1-LDH-Pt with a GLY conversion of 87.6% and a GLYA yield of 58.6%, which exceeded the traditional activated carbon and oxide supports. A combinative study on structural characterizations(XANES, CO-FTIR spectra, and benzoic acid titration) proves that a higher Mg/Al molar ratio promotes the formation of positively charged Ptd+species at metal-support interface, which accelerates bond cleavage of a-C–H and improves catalytic activity. Moreover, a higher Mg/Al molar ratio provides a stronger basicity of support that contributes to the oxidation of terminal-hydroxyl and thus enhances the selectivity of GLYA. This catalyst with tunable metal-support interaction shows prospective applications toward transformation of biomass-based polyols.

关键词： Rehydrated hydrotalcites Interfacial structure Support basicity Active sites Rate-determining step