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Macrophage Inflammatory Protein-lalpha mediates Matrix Metalloproteinase-9 enhancement in human adherent monocytes fed with malarial pigment

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Asian Pacific Journal of Tropical Medicine 2011年第12期4卷 925-930页

作者： Giuliana Giribaldi Elena Valente Amina Khadjavi manuela polimeni Mauro Prato Dipartimento di Genetica Biologia e BiochimicaFacolta di Medicina e ChirurgiaUniversita degli studi di TorinoTorinoItaly Dipartimento di Genetica Biologia e BiochimicaFacolta di Medicina e ChirurgiaUniversita degli studi di TorinoTurinoItaly Corresponding author:Prof.Mauro Prato PhDDepartment of GeneticsBiology and BiochemistryUniversity of Torino Medical School10126TorinoItaly

Objective:To investigate the role of macrophage inflammatory protein-1alpha(MIP-1 alpha) in the detrimental enhancement of matrix mnetalloproteinase-9(MMP-9) expression,release and activity induced by phagocytosis of malarial pigment(haemozoin,HZ) in human monocytes. Methods:Human adherent monocytes were unfed/fed with native HZ for 2 *** 24 hours. MIP-1 alpha production was evaluated by ELISA in cell *** /fed monocytes were treated in presence/absence of anti-human MIP-1 alpha blocking antibodies or recombinant human MIP-lalpha for 15 h(RNA studies) or 24 h(protein studies): therefore,MMP-9 mRNA expression was evaluated in cell lysatcs by Real Time RT-PCR,whereas proMMP-9 and active MMP-9 protein release were measured in cell supernatants by Western blotting and gelatin ***:Phagocytosis of HZ by human monocytes increased production of MIP-1 *** expression of MMP-9 and protein release of proMMP-9 and active *** the HZ-enbancing effects on MMP-9 were abrogated by anti-human MIP- 1 alpha blocking antibodies and mimicked by recombinant human MIP-l ***: The present work suggests a role for MIP-lalpha in the HZ-dependent enhancement of MMP-9 expression,release and activity observed in human *** new detrimental effects of HZ-triggered proinflammatory response by phagocytic cells in falciparum malaria.

关键词： Plasmodium falciparum Malaria Monocyte Phagocytosis Haemozoin Matrix metalloproteinase-9 Macrophage inflammatory proteinlalpha

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