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Estimate of the energy value of soybean meal relative to corn based on growth performance of nursery pigs

journal of Animal Science and Biotechnology 2020年第3期11卷 912-919页

作者： Henrique S.Cemin Hayden E.Williams Mike D.Tokach Steve S.Dritz jason C.Woodworth joel M.DeRouchey Robert D.Goodband Kyle F.Coble Brittany A.Carrender mandy j.gerhart Department of Animal Sciences and Industry College of AgricultureKansas State UniversityManhattanKS 66506USA Department of Diagnostic Medicine/Pathobiology College of Veterinary MedicineKansas State UniversityManhattanKS 66506USA jBS USA GreeleyCO 80634USA

Background:Two experiments were conducted to determine the effects of increasing amounts of soybean meal(SBM)in swine diets and estimate the energy value of ***:A total of 2233 pigs(PIC 337×1050,Hendersonville,TN)and 3796 pigs(PIC 359×C40),initially 11.0 kg and 17.6 kg body weight(BW),were used in Exp.1 and 2,*** Exp.1,pigs were placed in 92 pens each containing 20 to 27 *** Exp.2,pigs were placed in 84 pens each containing 37 to 43 *** were assigned in a randomized complete block design with BW as the blocking *** treatments consisted of 21%,27%,33%,or 39%SBM in Exp.1 and 17.5%,22%,26.5%,31%,35.5%,or 40%SBM in Exp.2,obtained by changing the inclusion rate of feed-grade amino acids and corn *** Exp.1,representative samples of corn grain,SBM,and distillers dried grains with solubles were analyzed for total AA content prior to diet *** Exp.2,diets were formulated using NRC(2012)nutrient *** diets were fed for 21 and 22 d(Exp.1 and 2)and there were 23 replicates in Exp.1 and 14 replicates in *** were weighed and feed disappearance measured weekly to calculate average daily gain(ADG),average daily feed intake(ADFI),gain-to-feed ratio(G:F),and caloric efficiency(CE).Data were analyzed with block as a random effect and treatment as a fixed effect,and contrasts were constructed to test the linear and quadratic effects of increasing ***:In Exp.1,there was a tendency(linear,P=0.092)for a decrease in ADFI as SBM *** was a tendency(P=0.090)for a quadratic response for ADG,with a decrease in ADG observed with 39%SBM *** fed diets with increasing SBM had a tendency(quadratic,P=0.069)for an increase in G:F up to 33%SBM and an improvement(linear,P=0.001;quadratic,P=0.063)in CE with increasing *** CE to estimate the energy of SBM relative to corn,a value of 105.4%of corn energy or 2816 kcal/kg NE was determined using all data *** removing the CE value

关键词： Caloric efficiency Energy Soybean meal Swine

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Estimate of the energy value of soybean meal relative to corn based on growth performance of nursery pigs.

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畜牧与生物技术杂志(英文版) 2020年第3期

作者： Henrique S.Cemin Hayden E.Williams Mike D.Tokach Steve S.Dritz jason C.Woodworth joel M.DeRouchey Robert D.Goodband Kyle F.Coble Brittany A.Carrender mandy j.gerhart

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The availability of probiotics and donor human milk is associated with improved survival in very preterm infants

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World journal of Pediatrics 2018年第5期14卷 492-497页

作者： janet Sharpe mandy Way Pieter j.Koorts Mark W.Davies Grantley Stable Neonatal Unit Department of NeonatologyRoyal Brisbane and Women’s HospitalButterfi eld StHerstonBrisbaneQLD 4029Australia Queensland Institute of Medical Research and Royal Brisbane and W omen’s Hospital Statistical Unit BrisbaneQLDAustralia Department of P ediatrics and Child Health The University of QueenslandBrisbaneQLDAustralia

Background To determine whether the introduction of pasteurized donor human milk and probiotics for infants born<32 weeks gestational age or<1500 g birthweight is associated with a reduction in mortality and the incidence of necrotising enterocolitis (NEC) and sepsis. Methods We performed a retrospective analysis of two cohorts: before and after the introduction of probiotics and pasteurised donor human milk. Univariate analysis of primary and secondary outcomes was performed;variables impacting outcomes were assessed using multivariate logistic regression. Results There were 1791 infants: 1334 in the pre-donor milk/probiotic cohort and 457 in the post-donor milk/probiotic cohort. On univariate analysis, mortality (7.6 vs. 2.4%, P<0.001) and incidence of sepsis (6.2 vs. 3.5%, P=0.028) were statistically significantly lower in the post-donor milk/probiotic group. NEC (2.8 vs. 1.5%, P=0.14) and non-NEC associ-ated gastrointestinal perforation (1.6 vs. 0.4%, P=0.052) were lower in the post-donor milk/probiotics cohort, but these were not statistically significant. The difference in mortality remained statistically significant on multivariate analysis in the post-donor milk/probiotic cohort compared to those in the pre-donor milk/probiotic cohort (odds ratio 0.31, 95% confidence interval 0.16–0.61). The decrease in the incidence of NEC was consistent with previous observational studies but the differ-ence was not statistically significant. Conclusion The availability of probiotics and pasteurised donor human milk is associated with a reduction in mortality in very preterm infants.

关键词： Enterocolitis necrotizing Infant newborn Milk human Milk banks Probiotics

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Human-Animal Interactions in Disaster Settings:A Systematic Review

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International journal of Disaster Risk Science 2023年第3期14卷 369-381页

作者： Haorui Wu Lindsay K.Heyland mandy Yung Maryam Schneider School of Social Work Faculty of HealthDalhousie UniversityHalifaxNS B3H 4R2Canada Department of Psychology Mount Saint Vincent UniversityHalifaxNSB3M2J6Canada

This systematic review aimed to assess the current knowledge of human-animal interactions(HAIs)in disaster settings and identify areas for future research.A Preferred Reporting Items for Systematic Reviews and Meta-Analyses search was conducted on three multidisciplinary databases,identifying English-language journal articles published between january 2000and February 2022 that explored the benefits of and challenges associated with HAI in disasters and *** review analyzed 94 articles using both quantitative and qualitative *** review found a paucity of universal terminology to describe the bidirectional relationship between humans and animals during disasters and a failure to include all animal types in every stage of disaster and emergency ***,research predominantly focused on the health and well-being benefits of HAI for humans rather than *** to promote social and environmental justice for humans and their co-inhabitants should support the welfare of both humans and animals in disaster *** recommendations were developed based on these findings to increase the inclusion of HAI in research,policy,and *** of the review included the exclusion of pre-2000 articles and all grey literature,limited research examining different combinations of animal and disaster types,and limited research outside of North America.

关键词： Emergency management Hazards and disaster research Human-animal bonds Human-animal interactions Human-animal welfare Inclusive Literature review

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Effect of toasting grain silages from field peas(Pisum sativum)and field beans(Vicia faba)on in vitro gas production,methane production,and post-ruminal crude protein content

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Animal Nutrition 2020年第3期6卷 342-352页

作者： Martin Bachmann Christian Kuhnitzsch Sebastian Michel Annabel Thierbach mandy Bochnia jorg M.Greef Siriwan D.Martens Olaf Steinhofel Annette Zeyner Institute of Agricultural and Nutritional Sciences Martin Luther University Halle-WittenbergHalle(Saale)06120Germany Saxon State Office for Environment Agriculture and GeologyKollitsch04886Germany julius Kühn Institute Federal Research Centre for Cultivated PlantsInstitute for Crop and Soil ScienceBraunschweig38116Germany

Legume grains such as field peas and field beans can be produced on a local level,and may be reliable sources of dietary protein and energy apart from common soybean and rapeseed *** ruminants,protein,starch,and carbohydrates from peas and field beans are fermented in large part before reaching the small *** objective of this study was to evaluate the effects of a combination of ensiling and hydro-thermic treatment(i.e.,toasting at 160℃for 30 min)of grains of peas and field beans on the concentrations of post-ruminal crude protein(PRCP)and rumen-undegraded protein(RUP).Moreover,24-h gas production and methane production were *** this,an in vitro batch culture system with ruminal fluid from sheep was ***-undegraded protein was determined using the Streptomyces griseus protease *** electron micrographs were used to visualize morphological changes of starch granules and their joint matrices in peas and field beans after ensiling,toasting,or a combination of *** pea grains contained crude protein(CP)at 199 g/kg DM,PRCP at 155 g/kg DM at a ruminal passage rate of 0.08/h(Kp8),RUP at 33 g/kg DM at Kp8,and starch at 530 g/kg *** field beans contained CP at 296 g/kg DM,PRCP at 212 g/kg DM at Kp8,RUP at 54 g of/kg DM at Kp8,and starch at 450 g/kg *** PRCP did not considerably differ among native and treated peas or field *** in the peas,RUP at Kp8 increased after ensiling by 10 g/kg DM(i.e.,30%;P<0.05).Toasting increased RUP(Kp8)in ensiled peas by another 28%(P<0.05).Toasting had no effect on PRCP or RUP when the peas or field beans were not ensiled *** and methane production were not affected by any treatment,and scanning electron micrographs did not reveal structural changes on the starches doubtless of any *** seemed to be more affected by treatment with ensiled+toasted peas than with ensiled+toasted field beans,but starches and other carbohydrates from both legumes remained unaffec

关键词： Legume grain silage Toasting In vitro gas production Protein evaluation Streptomyces griseus protease test

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Responses of Macroinvertebrate Community Metrics to a Wastewater Discharge in the Upper Blue River of Kansas and Missouri, USA

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journal of Water Resource and Protection 2015年第15期7卷 1195-1220页

作者： Barry C. Poulton jennifer L. Graham Teresa j. Rasmussen mandy L. Stone mandy L. Stone U.S. Geological Survey Columbia Environmental Research Center Columbia Missouri USA U.S. Geological Survey Kansas Water Science Center Lawrence Kansas USA

The Blue River Main wastewater treatment facility (WWTF) discharges into the upper Blue River (725 km2), and is recently upgraded to implement biological nutrient removal. We measured biotic condition upstream and downstream of the discharge utilizing the macroinvertebrate protocol developed for Kansas streams. We examined responses of 34 metrics to determine the best indicators for discriminating site differences and for predicting biological condition. Significant differences between sites upstream and downstream of the discharge were identified for 15 metrics in April and 12 metrics in August. Upstream biotic condition scores were significantly greater than scores at both downstream sites in April (p = 0.02), and in August the most downstream site was classified as non-biologically supporting. Thirteen EPT taxa (Ephemeroptera, Plecoptera, Trichoptera) considered intolerant of degraded stream quality were absent at one or both downstream sites. Increases in tolerance metrics and filtering macroinvertebrates, and a decline in ratio of scrapers to filterers all indicated effects of increased nutrient enrichment. Stepwise regressions identified several significant models containing a suite of metrics with low redundancy (R2 = 0.90 - 0.99). Based on the rapid decline in biological condition downstream of the discharge, the level of nutrient removal resulting from the facility upgrade (10% - 20%) was not enough to mitigate negative effects on macroinvertebrate communities.

关键词： Macroinvertebrates Metrics Wastewater Nutrients

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5-Fluorouracil-related enhancement of adenoviral infection is Coxsackievirus-adenovirus receptor independent and associated with morphological changes in lipid membranes

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World journal of Gastroenterology 2006年第32期12卷 5168-5174页

作者： Chiara Cabrele mandy Vogel Pompiliu Piso Markus Rentsch josef Schroder Karl W jauch Hans j Schlitt Alexander Beham Department of Surgery Clinic of the Regensburg University Franz-Josef-Strauss-Allee 11D-93053RegensburgGermany

AIM： To evaluate the mechanism underlying the effects of 5-Fluorouracil （5-FU） on adenoviral infection. METHODS： Low and high Coxsackievirus-Adenovirus Receptor （CAR） expressing human colon carcinoma cell lines were treated with 5-FU and two El-deleted adenoviral constructs, one transferring GFP （Ad/CMV- GFP） the other bax （Ad/CEA-bax）. The number of infected cells were monitored by GFP expression. To evaluate the effects of 5-FU in a receptor free system, Ad/GFP were encapsulated in liposomes and treated with 5-FU. Ad/GFP release was estimated with PCR and infection of 293 cells with the supernatant. Electron microscopy of the Ad5-GFP-liposome complex was made to investigate morphological changes of the liposomes after 5-FU. RESULTS： Infection rates of all cell lines increased from 50% to 98% with emerging 5-FU concentrations. The enhanced viral uptake was independent of the CAR expression. Additionally, 5-FU treated liposomes released 2-2.5 times more adenoviruses. Furthermore, 5-FU- treated liposomes appeared irregular and porous-like. CONCLUSION： adenoviral uptake is enhanced in the presence of 5-FU irrespective of CAR and is associated with morphological changes in membranes making the combination of both a promising option in gene therapy.

关键词： 5-Fluorouracil Coxsackievirus-adenovirusreceptor Adenoviral infection Gene therapy

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如何对待物理教学中的各种数量问题

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国际物理教育通讯 1991年第8期 1-6页

作者： j．B．gerhart 罗星凯周永平华盛顿大学广西师范大学

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知识产权许可策略探讨

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电子知识产权 2009年第7期 67-70页

作者： Esther H.Lim mandy j.Song 美国飞翰律师事务所

中国已成为一个巨大的知识产权许可市场。中国企业在考虑将知识产权许可作为知识产权管理战略的一部分时,有必要做出几个关键决策:是否该取得许可、许可内容以及许可来源。知识产权许可有助于中国公司取得有价值的技术、节省研发费用并... 详细信息

中国已成为一个巨大的知识产权许可市场。中国企业在考虑将知识产权许可作为知识产权管理战略的一部分时,有必要做出几个关键决策:是否该取得许可、许可内容以及许可来源。知识产权许可有助于中国公司取得有价值的技术、节省研发费用并避免商业化的延宕。

关键词：知识产权许可来源许可内容

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小反刍兽疫病毒(PPRV)N蛋白的表达与ELISA检测方法的建立

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中国兽医学报 2008年第2期28卷 146-148,156页

作者：张喜悦王志亮刘春菊李林包静月何昭阳 mandy C Carrie B Anderson j 吉林农业大学动物科技学院吉林长春130118 中国动卫中心外来病诊断中心山东青岛266032 动物卫生所英国伦敦GU24 ONF

通过对下载的16株小反刍兽疫病毒(PPRV)N基因片段进行序列分析,合成了PPRV N基因。用NP1和NP2引物扩增。纯化的PPRV N基因在T4DNA连接酶的作用下,和PET32-a载体进行连接反应构建重组质粒pET-a-PPRV-N。经诱导表达和纯化后,得到了大量的P... 详细信息

通过对下载的16株小反刍兽疫病毒(PPRV)N基因片段进行序列分析,合成了PPRV N基因。用NP1和NP2引物扩增。纯化的PPRV N基因在T4DNA连接酶的作用下,和PET32-a载体进行连接反应构建重组质粒pET-a-PPRV-N。经诱导表达和纯化后,得到了大量的PPRV N蛋白。用PPRV N蛋白制备免疫血清,经国际标准血清标化后作为参考阳性血清,同时以PPRV N蛋白为抗原建立间接ELISA(I-ELISA)方法。对山东省的467份羊血清检测,使用S/P(样品值/阳性值)平均值加3倍标准差的方法,计算出其临界点为0.28。应用间接ELISA和针对H蛋白的单抗为基础的C-ELISA检测来自于疫区的69份血清,结果两者的符合率为79.7%。

关键词： PPRV N蛋白表达 ELISA

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