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Screening for Monoclonal Antibodies Against the Membrane Proteins of Chicken Embryo Fibroblast Blocking the Infection of IBDV

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Agricultural Sciences in China 2010年第2期9卷 294-298页

作者： YANg Yan-yan ZHU Li-qian YANg Ji-fei TENg man CAI Shu-jun WANg Li LUO Jun DENg Rui-guang ZHANg gai-ping Henan Provincial Key Laboratory of Animal Immunology Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China College of Veterinary Medicine Yangzhou University Yangzhou 225009 P.R.China

This article aims to establish an efficient assay for screening monoclonal antibodies （McAbs） against the membrane proteins of chicken embryo fibroblast （CEF） for further studies of the cellular receptors of infectious bursal disease virus （IBDV）. McAbs against the membrane proteins of CEF were prepared by cell fusion. The monolayer CEF pre-incubated with the CEF-specific McAbs for 2 h were infected with IBDV and incubated with F22-EA6-biotin postinfection. Then, the cells were reacted with streptavidin-horseradish peroxidase （HRP） and finally stained by 3-amino-9-ethylcarbazole （AEC）. The inhibitive percentage of IBDV infection was calculated by counting the IBDV-infected cells to determine the inhibition efficiency of the CEF-specific McAbs. Compared with the control cells, the IBDV-infected cells pretreated with CEF-specific antibody significantly decreased; supernatant fluids of a total of 768 hybridomas were analyzed. The results of immunohistochemistry assays showed that six of them （1A5, 1H11, 2B 12, 3g1, 4D10, and 4B8） have the abilities to block the infection of IBDV to CEF, among which 4B8 can perfectly block the infection. This novel method is a sensitive and specific assay for the screening of CEF membrane protein-specific McAbs, which can block the infection of IBDV to CEF, and these McAbs can be used for the further investigations of the cellular receptors of IBDV.

关键词： CEF IBDV receptor biotin-streptavidin

Physicochemical properties, fatty acid and mineral content of some walnuts (Juglans regia L.) types

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Agricultural Sciences 2010年第2期1卷 62-67页

作者： Mehmet Musa Ozcan Cesariİman Derya Arslan Department of Soil Science Faculty of AgricultureUniversity of SelcukKonyaTurkey Food Engineering KirsehirTurkey

Some physical and chemical properties, mineral content and fatty acid compositions of kernel and oils of several walnut types (Büyük Oba, Kaman2, Kaman5) were determined. The oil yields from these kernels changed between 61.4% to 72.8%. The crude fibre contents of ker nels ranged between 3.77% and 3.80%. In add ition, crude protein contents of kernels ranged between 7.05% and 8.10%. While the peroxide values of kernel oils change between 3.18 meq/ Kg and 3.53 meq/Kg, acidity values ranged be tween 0.35% and 0.56%. The main fatty acids of walnut kernel oils were oleic, linoleic, linolenic and palmitic acids. Linoleic acid contents of ker nel oils varied between 49.7% and 55.5%. On the other hand, oleic acid contents ranged between 20.5% and 26.4%. As a result, the present study showed the walnut kernels of the researched species of walnut kernels from Turkey are a potential source of valuable oil which might be used for edible and other industrial applica tions.

关键词： Walnut Kernel Oil Fatty Acid Composition Mineral Contents

带双参数的脉冲泛函微分方程正周期解的存在性

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山东大学学报（理学版） 2015年第6期50卷 69-74,82页

作者：徐嫚西北师范大学数学与统计学院甘肃兰州730070

研究了带双参数的脉冲泛函微分方程u'(t)=h(t,u(t))-λf(t,u(t-τ(t))),t∈R,t≠tk,u(t+k)-u(tk)=μIk(tk,u(tk-τ(tk)))正周期解的存在性,其中λ>0,μ≥0为参数,获得了其在更一般条件下正周期解的存在性结果。主要结果的证明基于不动... 详细信息

研究了带双参数的脉冲泛函微分方程u'(t)=h(t,u(t))-λf(t,u(t-τ(t))),t∈R,t≠tk,u(t+k)-u(tk)=μIk(tk,u(tk-τ(tk)))正周期解的存在性,其中λ>0,μ≥0为参数,获得了其在更一般条件下正周期解的存在性结果。主要结果的证明基于不动点指数理论。

关键词：脉冲泛函微分方程双参数正周期解不动点指数

奇异Φ-Laplacian周期边值问题解的存在性

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山东大学学报（理学版） 2015年第8期50卷 72-77页

作者：徐嫚西北师范大学数学与统计学院甘肃兰州730070

考虑了奇异Φ-Laplacian周期边值问题{(Φ(u'))'+g(u)=s+e(t),t∈[0,T],u(0)-u(T)=0=u'(0)-u'(T)解的存在性,其中Φ:(-a,a)→R是单调递增的同胚且Φ(0)=0,0g∈C(R,R),e∈C[0,T],s是一个参数。主要结果的证明基于紧集连通理论及Le... 详细信息

关键词： Ф-Laplacian 周期边值问题紧集连通理论 Leray—Schauder度

High-performance ZnO humidity sensor synthesized by coprecipitation with PVP as surfactant for human respiration detection

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Optoelectronics Letters 2023年第1期19卷 1-7页

作者： man Jianping gU Weiyuan HU Ziyan DOU Xiaozong ZHANg Hongyan Xinjiang Key Laboratory of Solid State Physics and Devices Xinjiang UniversityUrumqi830046China School of Physics Science and Technology Xinjiang UniversityUrumqi830046China

A high-performance zinc oxide(ZnO)/polyvinylpyrrolidone(PVP)humidity sensor was prepared by simple coprecipitation method with PVP as *** coprecipitation method has low reaction temperature,little energy consumption and simple preparation,which is suitable for large-scale *** PVP makes sample’s surface with more hydroxyl and oxygen vacancies than pure ZnO,which can absorb more water molecules and promote the decomposition of water molecules into H3O+to form effective ion *** the molar ratio of PVP to ZnO is 1:1,the ZnO/PVP humidity sensor has low hysteresis(~4.2%),short response/recovery time(9/10 s),excellent stability and high sensitivity with more than 4 orders of magnitude in relative humidity(RH)range from 11%to 95%.Moreover,the ZnO/PVP humidity sensor can distinguish different respiratory states of human body,which has a potential in monitoring and prevention of respiratory diseases.

关键词： humidity performance surfactant

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小区域研究的信息化：数据架构及模型

中国历史地理论丛 2008年第2期23卷 5-11页

作者：满志敏复旦大学历史地理研究中心上海200433

区域内人地关系的变化过程研究是一个前沿课题,人与自然相互作用最强烈的地区成为许多重大课题的关注目标。信息化条件下的区域研究要求从空间差异和时间变化这三个维度描述人地关系的相互变化。本文讨论了小区域数据描述要求的载体数据... 详细信息

区域内人地关系的变化过程研究是一个前沿课题,人与自然相互作用最强烈的地区成为许多重大课题的关注目标。信息化条件下的区域研究要求从空间差异和时间变化这三个维度描述人地关系的相互变化。本文讨论了小区域数据描述要求的载体数据,从载体数据的意义、空间尺度、稳定性要求、可能性要求等方面讨论了运用载体数据的一些需要关注的问题。区域描述的专题数据有许多来源,需要一定的架构方法整合,因此相关的数据标准化处理和定义,以及数据在时间变化上的定义成了架构数据的重要问题。

关键词：区域研究人地关系数据架构载体数据

Role of diabetes mellitus on the recurrence rate of hepatocellular carcinomas after radiofrequency ablation in chronic hepatitis C patients

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Hepatoma Research 2015年第1期1卷 24-29页

作者： Abd Allah Ahmed el-Sawy Mohamed Abd el-Raouf Tawfik Hepatogastroenterology and Endoscopy Unit Internal Medicine DepartmentTanta University HospitalTanta 31527Egypt

Aim:The aim was to assess the impact of hyperglycemia on the recurrence of hepatocellular carcinoma(HCC)as well as evaluate survival after curative ablation by ***:This study,which was conducted retrospectively on 107 chronic hepatitis C(CHC)patients with 159 HCCs,was presented to the Hepatology Unit of Internal Medicine Department at Tanta University *** lesions were curatively treated by radiofrequency ablation(RFA)and the surveillance of HCC recurrence was evaluated radiologically every 3 months for periods between 6 and 36 *** 107 subjects,70 were males and 37 were females,with mean age 50.4±9.4 *** patients were divided according to their glycemic state into the following three groups:group I,which included 37 type 2 diabetic patients,with adequate maintenance of blood glucose,has 52 HCCs;group II,which included 25 type 2 diabetic patients with inadequate maintenance of blood glucose,has 43 HCCs;and group III,which included 45 euglycemic non-diabetic patients,has 64 ***:Our results showed that,there was signifi cant increase in recurrence rate in diabetic patients with inadequate maintenance of blood glucose(group II)compared to those in group I and group III(Pgly,there was no signifi cant difference concerning HCC recurrence between diabetic patients with adequate maintenance of blood glucose(group I)and non-diabetic euglycemic patients(group III).Our results also identifi ed that,inadequate maintenance of blood glucose in diabetic patients was also a signifi cant predictor of poor ***:Inadequate maintenance of blood glucose in diabetic patients is a signifi cant risk factor for recurrence of HCC and for poor survival after curative RFA therapy in CHC patients.

关键词： Hepatocellular carcinoma hyperglycemia radio frequency ablation recurrence survival

CH_(4)/NO_(x) Reduced Mechanisms Used for Modeling Premixed Combustion

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Energy and Power Engineering 2012年第4期4卷 264-273页

作者： Abdellatif Belcadi Mohammed Assou el Houssine Affad el Houssine Chatri Department of Physics Faculty of SciencesMoulay Ismail UniversityMeknèsMorocco Laboratory of Heat Transfer and Mass FST of MohammadiaHassan II UniversityMohammadiaMorocco

This study has identify useful reduced mechanisms that can be used in computational fluid dynamics (CFD) simulation of the flow field, combustion and emissions of gas turbine engine combustors. Reduced mechanisms lessen computational cost and possess the ability to accurately predict the overall flame structure, including gas temperature and species as CH4, CO and NOx. The S-STEP algorithm which based on computational singular perturbation method (CSP) is performed for reduced the detailed mechanism gRI-3.0. This algorithm required as input: the detailed mechanism, a numerical solution of the problem and the desired number of steps in the reduced mechanism. In this work, we present a 10-Step reduced mechanism obtained through S-STEP algorithm. The rate of each reaction in the reduced mechanism depends on all species, steady-state and non-steady state. The former are calculated from the solution of a system of steady-state algebraic relations with the point relaxation algorithm. Based on premixed code calculations, The numeric results which were obtained for 1 atm ≤ Pressure ≤ 30 atm and 1.4 ≤ ф ≤ 0.6 on the basis of the ten steps global mechanism, were compared with those computed on the basis of the detailed mechanism gRI-3.0. The 10-step reduced mechanism predicts with accuracy the similar results obtained by the full gRI-3.0 mechanism for both NOx and CH4 chemistry.

关键词： CSP Method S-STEP Algorithm Reduced Mechanism Methane and Premixed Laminar Flame

Primary Hyperparathyroidism in a Twin Pregnancy: A Case Report

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Open Journal of Endocrine and Metabolic Diseases 2016年第6期6卷 167-171页

作者： Luis Eduardo Pérez Sánchez Julio César Jordan Balanza Alicia goya Pacheco P.E.g. de Chaves Rodríguez man.el Barrera gómez Endocrine Surgery Unit Department of General and Digestive Surgery Hospital Universitario Nuestra Se?ora de Candelaria Tenerife Spain

The primary hyperparathyroidism during pregnancy is an infrequent disorder but it is rarer in a twin gestation. Often it is not suspected because the symptomatology is overlapped with the normal course of pregnancy and it is usually discovered after a routine blood test. Ultrasound is the best option to identify the adenoma, while the gold-standard is technetium-99 m sestamibi but is contraindicated in this situation. The treatment is controversial and depends of the time of the gestation: the medical therapy is accepted in the first and third trimester, and the surgery is reserved for the second trimester, where the side effects are low. We present a case of primary hyperparathyroidism in a twin pregnancy treated by a minimally invasive parathyroidectomy in the second trimester without consequences for both, mother and newborns.

关键词： Hyperparathyroidism Pregnancy Surgery