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Science China Chemistry 2018年第9期61卷 1151-1158页

作者： man gao Xiaowu Liu Hai Yang Yan Yu Key Laboratory of Materials for Energy Conversion Chinese Academy of Sciences(CAS) Department of Materials Science and EngineeringUniversity of Science and Technology of China

Double carbon coated Fe P composite(Fe P@NC@r GO)was in situ fabricated via the phosphorization process of the as-prepared Prussian blue@graphene oxide(PB@GO)*** Fe P nanocrystals were successfully embedded in the nitrogen-doped porous carbon *** used as the anode for lithium ion batteries(LIBs),the Fe P@NC@r GO anode shows superior lithium storage properties,delivering a high specific capacity of 830 m A h g^(-1)after 100 cycles at 100 m A g^(-1)and excellent rate capability of 359 m A h g^(-1)at 5 A g^(-1).The outstanding performance mainly ascribes to the synergistic effect of the double carbon coating and porous structure *** introduction of porous carbon and graphene coating on Fe P nanoparticles greatly enhance the electronic conductivity of the active material and well accommodates the large volume variation of Fe P during the cycling process.

关键词： FeP Prussian blue reduced graphene oxide anode lithium ion battery

2D sandwich-like nanosheets of ultrafine Sb nanoparticles anchored to graphene for high-efficiency sodium storage

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Nano Research 2017年第12期10卷 4360-4367页

作者： Xiaowu Liu man gao Hai Yang Xiongwu Zhong Yan Yu CAS Key Materiels for Energy Conversion Department of Materials Science and Engineering University of Science and Technology of Chine Hefei 230026 China Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University Tianjin 300071 China State Key Laboratory of Fire Science University of Science and Technology of China Hefei 230026 China

Sb is considered a promising anode material for high-performance sodium-ion batteries （NIBs） owing to its high theoretical specific capacity （660 mAh-g-1）. However, Sb shows a very large volume change （-200%） during sodiation and desodiation, leading to poor electrochemical performance. Here, we designed and tested a sandwich-like graphene-supported Sb nanocomposite （denoted Sb@RGO@Sb）, in which ultrafine Sb nanoparticles are uniformly anchored on a reduced graphene oxide （RGO） surface. The ultrafine Sb nanocrystals anchored on the RGO surface minimize the aggregation of Sb and inhibit restacking of the RGO sheets, leading to a minimum transport length for both ions and electrons. The graphene layer not only accommodates the large volume variation of Sb during cycling but also promotes the electron conductivity of the whole electrode. Owing to its unique structure, this sandwich-like composite exhibits superior sodium storage properties.

关键词： Sb/graphene composites sodium ion battery anode sandwich-like structure

Gene Clone and Characterization of a Novel Thermostable β-Galactosidase with Transglycosylation Activity from Thermotoga naphthophila RUK-10

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Chemical Research in Chinese Universities 2015年第4期31卷 564-568页

作者： YANG Jingwen DI Xiangjun WANG man gao Renjun Key Laboratory for Molecular Enzymology and Engineering Ministry of Education School of Life Science Jilin University Changchun 130012 P. R. China

We cloned and expressed a new recombinant β-galactosidase（TN0949） from Thermotoga naphthophila RKU-10 with the pET28a（＋） vector system in Escherichia coli BL21（DE3）, and determined its catalytic capability to synthesize alkyl glucosides. The recombinant enzyme was purified to a single band via heat treatment and Ni2＋-NTA affinity chromatography. The molecular mass of the recombinant enzyme was estimated to be 79 kDa with sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE）. TN0949 can hydrolyze o-nitrophenylβ-D- galactopyranoside at the optimum pH and temperature of 6.5 and 80 ℃, respectively. TN0949 can also hydrolyze lactose at the optimum pH and temperature of 5.2 and 80 ℃, respectively. The Km values for the hydrolyses of o-nitrophenyl fl-D-galactopyranoside and lactose were 0.82 and 83.65 mmol/L, respectively. TN0949 was stable over a wide range of pH（3.0 to 7.0） after 24 h of incubation. The half-lives of TN0949 at 75, 80 and 85 ℃ were 22, 6 and 1.33 h, respectively. The enzyme displayed the capability to use lactose as the transglycosylation substrate to synthesize butyl galactopyranoside and hexyl galactopyranoside, indicating its suitability as a candidate industrial biocatalyst.

关键词： β-Galactosidase Thermostable Transglycosylation

基于多特征融合卷积神经网络结合Transformer的电能质量扰动分类方法

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北华大学学报（自然科学版） 2025年第1期26卷 115-124页

作者：王高峰张卓石高蔓钱云北华大学电气与信息工程学院吉林吉林132021

随着可再生能源发电技术的发展,越来越多的可再生能源和设备应用到电力系统中,使电能质量扰动(Power Quality Disturbances,PQDs)发生频率显著增加。PQDs的准确分类对于研究PQDs发生原因和预防至关重要。提出基于多特征融合的卷积神经网... 详细信息

随着可再生能源发电技术的发展,越来越多的可再生能源和设备应用到电力系统中,使电能质量扰动(Power Quality Disturbances,PQDs)发生频率显著增加。PQDs的准确分类对于研究PQDs发生原因和预防至关重要。提出基于多特征融合的卷积神经网络(CNN)结合Transformer模型(CNN-Transformer)对PQDs进行分类。利用快速傅里叶变换(fast Fourier transform,FFT)从PQDs时间序列中提取频域信息,使用CNN-Transformer模型分别对PQDs的时域和频域信息进行特征提取,实现PQDs识别分类。使用该模型对16种合成PQDs数据进行仿真,结果显示:该模型在无噪声条件下的分类准确率为99.88%,在噪声条件下准确率在98.00%以上,且拥有良好的抗噪性和泛化性能。与现有部分分类模型比较显示,本文模型在对比的模型中性能最优。

关键词：电能质量扰动分类时频分析卷积神经网络多头注意力机制

Transcriptomic analysis of spinal cord regeneration after injury in Cynops orientalis

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Neural Regeneration Research 2023年第12期18卷 2743-2750页

作者： Di Wang man Zhao Xiao Tang man gao Wenjing Liu Minghui Xiang Jian Ruan Jie Chen Bin Long Jun Li College of Life Sciences Anhui Normal UniversityWuhuAnhui ProvinceChina College of Animal Science and Technology Nanjing Agricultural UniversityNanjingJiangsu ProvinceChina Laboratory of Reproductive Medicine The Second People’s HospitalWuhuWuhuAnhui ProvinceChina

Cynops orientalis(***)has a pronounced ability to regenerate its spinal cord after ***,exploring the molecular mechanism of this process could provide new approaches for promoting mammalian spinal cord *** this study,we established a model of spinal cord thoracic transection injury in ***,which is an endemic species in *** performed RNA sequencing of the contused axolotl spinal cord at two early time points after spinal cord injury–during the very acute stage(4 days)and the subacute stage(7 days)–and identified differentially expressed genes;additionally,we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses,at each time *** sequencing showed that 13,059 genes were differentially expressed during *** spinal cord regeneration compared with uninjured animals,among which 4273 were continuously downregulated and 1564 were continuously ***-regulated genes were most enriched in the Gene Ontology term“multicellular organismal process”and in the ribosome pathway at 10 days following spinal cord *** found that multiple genes associated with energy metabolism were down-regulated and multiple genes associated with the lysosome were up-regulated after spinal cord injury,indicating the importance of low metabolic activity during wound *** response-associated pathways were activated during the early acute phase(4 days),while the expression of extracellular matrix proteins such as glycosaminoglycan and collagen,as well as tight junction proteins,was lower at 10 days post-spinal cord injury than 4 days post-spinal cord ***,compared with 4 days post-injury,at 10 days post-injury neuroactive ligand-receptor interactions were no longer down-regulated,up-regulated differentially expressed genes were enriched in pathways associated with cancer and the cell cycle,and SHH,VIM,and Sox2 were prominently *** staining showed

关键词： Cynops orientalis extracellular matrix glial fibrillary acidic protein metabolism neuron RNA sequence salamander spinal cord injury spinal cord regeneration Transcriptomics

Celastrol inhibits inflammatory factors expression in glioblastoma

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Traditional Medicine Research 2024年第6期9卷 1-11页

作者： Na Qin Shu-Xian Yang Shi-man gao Ting Zhu Institute of Neuroregeneration&Neurorehabilitation Department of PathophysiologySchool of Basic MedicineQingdao UniversityQingdao 266071China Department of Pharmacy Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China Women and Children’s Hospital Qingdao UniversityQingdao 266034China

Background:Glioblastoma is one of the most common primary intracranial tumors of the central nervous system in *** chemotherapy is an important component of glioblastoma treatment,its effectiveness remains *** to multiple immunosuppressive mechanisms,glioblastoma immunotherapy has not been effective in treating many patients as a result of the clinical breakthroughs in the ***,the development of cancer immunotherapy relies on the understanding of how tumors interact with the immune system and the analysis of their molecular *** study identified the key interactions between immune cells in the glioma microenvironment using RNA microarrays and single-cell ***:First,we screened differentially expressed genes in tumor and control samples from GSE29796 and GSE50161 datasets using *** differentially expressed genes were used to perform enrichment analysis and construct protein-protein interaction topological analysis to analyze the interaction between *** single-cell RNA sequencing data from the GSE162631 database,we identified immune cell types within the glioblastoma microenvironment,and validated the hub gene expression in these *** addition,based on the GEPIA and TIMER databases,hub genes were investigated and compared with immune infiltration to determine differential ***,CellChat was used to visualize the gene expression distribution and cell-to-cell communication analysis of the proteins between different types of ***:We found that monocytes/macrophages may communicate with each other in the tumor microenvironment through MIF-(CD74+CXCR4)and MIF-(CD74+CD44).In addition,our study indicated that celastrol has the ability to inhibit inflammatory factors expression by MIF/CD74 signaling pathway in U87 ***:This study improved the effectiveness of cancer immunotherapy strategies and developed new ideas for immunotherapy that can be applied to gl

关键词： glioblastoma monocytes/macrophages single-cell RNA sequencing MIF/CD74 signaling pathway celastrol

Inhibition of bromodomain-containing protein 4 enhances the migration of esophageal squamous cell carcinoma cells by inducing cell autophagy

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World Journal of Gastrointestinal Oncology 2022年第12期14卷 2340-2352页

作者： Wen-Qian Yang Rui Liang man-Qi gao Yu-Zhen Liu Bo Qi Bao-Sheng Zhao Department of Thoracic Surgery The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina Esophageal Cancer Institute Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina Life Science Research Center The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina

BACKGROUND Esophageal squamous cell carcinoma(ESCC),the predominant type of esophageal cancer,has a 5-year survival rate less than 20%.Although the cause of poor prognosis is the high incidence and mortality of ESCC,the high rate of metastasis after esophageal cancer surgery is the main cause of death after the ***-containing protein 4(BRD4),an epigenetic reader of chromatinacetylated histones in tumorigenesis and development,plays an essential role in regulating oncogene ***4 inhibition and BRD4 inhibition-based treatment can potentially suppress ESCC ***,the effects and mechanisms of action of BRD4 on ESCC cell migration remain *** To explore the effect of BRD4 on cell migration of ESCC in vitro and its possible molecular *** Human ESCC cell lines KYSE-450 and KYSE-150 were *** 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was performed to examine cell proliferation,and the transwell migration assay was conducted to test ESCC cell ***1,a BRD4 inhibitor,was applied to cells,and BRD4 siRNA was transfected into ESCC cells to knockdown endogenous ***-RFP-LC3 adenovirus was infected into ESCC cells to evaluate the effect of JQ1 on *** blotting was performed to determine the protein levels of BRD4,E-cadherin,vimentin,AMP-activated protein kinase(AMPK),and *** BRD4 was either downregulated by small interfering RNA or pretreated with JQ1 in ESCC cells,leading to increased tumor migration in ESCC cells in a dose-and time-dependent *** of BRD4 not only significantly suppressed cell proliferation but also strongly increased cell migration by inducing epithelial-mesenchymal transition(EMT).The protein expression of vimentin was increased and E-cadherin decreased in a dose-dependent manner,subsequently promoting autophagy in KYSE-450 and KYSE-150 *** with JQ1,a BRD4 inhibitor,inhibited BRD4-induced LC3-II activation and

关键词： JQ1 Bromodomain-containing protein 4 Cell migration Cell autophagy Epithelialmesenchymal transition Esophageal squamous cell carcinoma

Studying Chinese Characters in a Web-Based Learning Environment: A Case Study of Swedish University Students

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汉语教学方法与技术 2020年第2期3卷 1-16页

作者： Tao Yang man gao Dalarna University Sweden

This survey study aims to investigate the perceptions learners have of the character-learning strategies they employ when taking a web-based course in *** seven Likert-scale statements are included in the questionnaire to examine learners’opinions on the three character-learning strategies that are widely used in traditional campus courses.A total of 65 students who completed the beginner level‘Chinese Characters’web-based course at a university in Sweden completed the *** results suggest that students in web-based courses consider these three strategies to be just as helpful and effective as campus students;moreover,the more orthodox strategy-rote learning-is found to be the most popular among distance ***,findings of this study provide insight into the limitations and advantages associated with a web-based course,and also the possible effect learners’age and gender may have on learning strategy preferences.

关键词： Chinese characters character-learning strategy web-based courses distance education

Time-sequential changes of differentially expressed miRNAs during the process of anterior lumbar interbody fusion using equine bone protein extract, rhBMP-2 and autograft

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Frontiers of Materials Science 2014年第1期8卷 72-86页

作者： Da-Fu CHEN Zhi-Yu ZHOU Xue-Jun DAI man-man gao Bao-Ding HUANG Tang-Zhao LIANG Rui SHI Li-Jin ZOU Hai-Sheng LI Cody BUNGER Wei TIAN Xue-Nong ZOU Laboratory of Bone Tissue Engineering Beijing Research Institute of Traumatology and Orthopaedics Beijing Jishuitan Hospital Beijing 100035 China Department of Spine Surgery/Orthopaedic Research Institute The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080 China Department of Surgery the First Affiliated Hospital of Nanchang University Nanchang 330006 China Orthopaedic Research Laboratory/The Institute of Clinical Medicine Aarhus University Hospital DK-8000 Aarhus Denmark Medicine School Shenzhen University Shenzhen 518060 China

The precise mechanism of bone regeneration in different bone graft substitutes has been well studied in recent researches. However, miRNAs regulation of the bone formation has been always mysterious. We developed the anterior lumbar interbody fusion （ALIF） model in pigs using equine bone protein extract （BPE）, recombinant human bone morphogenetic protein-2 （rhBMP-2） on an absorbable collagen sponge （ACS）, and autograft as bone graft substitute, respectively. The miRNA and gene expression profiles of different bone graft materials were examined using microarray technology and data analysis, including self-organizing maps, KEGG pathway and Biological process GO analyses. We then jointly analyzed miRNA and mRNA profiles of the bone fusion tissue at different time points respectively. Results showed that miRNAs, including let-7, miR-129, m iR-21, miR-133, miR-140, miR-146, miR-184, and miR-224, were involved in the regulation of the immune and inflammation response, which provided suitable inflammatory microenvironment for bone formation. At late stage, several miRNAs directly regulate SMAD4, Estrogen receptor 1 and 5-hydroxytryptamine （serotonin） receptor 2C for bone formation. It can be concluded that miRNAs play important roles in balancing the inflammation and bone formation.

关键词： miRNA bone protein extract (BPE) recombinant human bone morphoge-netic protein-2 (rhBMP-2) autograft

Diagnosis based on electromagnetic navigational bronchoscopyguided biopsied peripheral lung lesions in a 10-year-old girl:A case report

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World Journal of Clinical Cases 2023年第15期11卷 3651-3657页

作者： Fan-Zheng Meng Qiong-Hua Chen man gao Li'e Zeng Jie-Ru Lin Jing-Yang Zheng Department of Pediatrics The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Respiratory Medicine The Third Clinical Medical CollegeFujian Medical UniversityQuanzhou Women's and Children's HospitalQuanzhou 362000Fujian ProvinceChina Department of Respiratory Medicine Quanzhou Women's and Children's HospitalQuanzhou 362000Fujian ProvinceChina Department of Respiratory Medicine Quanzhou Women's and Children's HospitalThe Third Clinical Medical CollegeFujian Medical UniversityQuanzhou 362000Fujian ProvinceChina

BACKGROUND Electromagnetic navigational bronchoscopy(ENB)is an emerging diagnostic tool that enables practitioners to biopsy peripheral lung tissues that were previously only accessible under computed tomography(CT)***,few studies have investigated ENB use in ***,we report a case of a 10-yearold girl with peripheral lung lesions who complained of a 7-d persistent *** was diagnosed with Streptococcus parasanguinis infection based on findings obtained using ENB-guided transbronchial lung biopsy(TBLB).CASE SUMMARY A 10-year-old girl presented with constitutional symptoms of cough and fever of 7 days’*** CT scans detected peripheral lung lesions and no endobronchial *** performed under the guidance of an ENB Lungpro navigation system was safe,well-tolerated,and effective for biopsying peripheral lung *** of biopsied samples indicated the patient had a pulmonary Streptococcus parasanguinis infection,which was treated with antibiotics instead of more invasive treatment *** patient’s symptoms resolved after she received a 3-wk course of oral *** of pretreatment and post-treatment CT scans revealed absorption of some lung lesions within 7 mo of hospital *** ENB-guided TBLB biopsying of peripheral lung lesions in this child is a safe,well-tolerated,and effective alternative to conventional interventions.

关键词： Electromagnetic navigational bronchoscopy Lungpro Transbronchial lung biopsy Child Diagnosis Case report