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Classification of Plant Endogenous States Using Machine Learning-Derived Agricultural Indices

Plant Phenomics 2023年第3期5卷 474-483页

作者： Sally Shuxian Koh Kapil Dev Javier Jingheng Tan Valerie Xinhui Teo Shuyan Zhang Dinish U.S. malini olivo Daisuke Urano Temasek Life Sciences Laboratory National University of SingaporeSingaporeSingapore Department of Biological Sciences National University of SingaporeSingaporeSingapore Translational Biophotonics Laboratory Institute of Bioengineering and BioimagingAgency for ScienceTechnology and Research(A*STAR)SingaporeSingapore Institute of Materials Research and Engineering(IMRE) Agency for ScienceTechnology and Research(A*STAR)SingaporeSingapore

Leaf color patterns vary depending on leaf age,pathogen infection,and environmental and nutritional stresses;thus,they are widely used to diagnose plant health statuses in agricultural *** visible-near infrared-shortwave infrared(VIS-NIR-SWIR)sensor measures the leaf color pattern from a wide spectral range with high spectral ***,spectral information has only been employed to understand general plant health statuses(e.g.,vegetation index)or phytopigment contents,rather than pinpointing defects of specific metabolic or signaling pathways in ***,we report feature engineering and machine learning methods that utilize VIS-NIR-SWIR leaf reflectance for robust plant health diagnostics,pinpointing physiological alterations associated with the stress hormone,abscisic acid(ABA).Leaf reflectance spectra of wild-type,ABA2-overexpression,and deficient plants were collected under watered and drought ***-and ABA-associated normalized reflectance indices(NRIs)were screened from all possible pairs of wavelength *** associated NRIs showed only a partial overlap with those related to ABA deficiency,but more NRIs were associated with drought due to additional spectral changes within the NIR wavelength *** support vector machine classifiers built with 20 NRIs predicted treatment or genotype groups with an accuracy greater than those with conventional vegetation *** selected NRIs were independent from leaf water content and chlorophyll content,2 well-characterized physiological changes under *** screening of NRIs,streamlined with the development of simple classifiers,serves as the most efficient means of detecting reflectance bands that are highly relevant to characteristics of interest.

关键词： drought reflectance indices

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Micro-and nano-fiber probes for optical sensing,imaging,and stimulation in biomedical applications

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Photonics Research 2020年第11期8卷 1703-1724页

作者： Xia Yu Shuyan Zhang malini olivo Nanxi Li School of Instrumentation and Optoelectronic Engineering Beihang UniversityBeijing 100083China Singapore Bioimaging Consortium Agency for ScienceTechnologyand Research(A*STAR)Singapore 138667Singapore Institute of Microelectronics Agency for ScienceTechnologyand Research(A*STAR)Singapore 138634Singapore

The flexibile nature of optical fiber enables it to offer remote-access capabilities,which could be used in many biomedical *** review focuses on different micro-and nano-structured fiber probes for applications in biosensing,imaging,and *** modifications to fiber could extend design freedom from waveguide optimization to functional material *** probes with optimized waveguide structures or integrated functional materials could achieve enhanced optical mode interaction with biosamples,and hence obtain ultrasensitive biosensors with a remarkably low limit of ***,bioimaging with a high spatial resolution can be obtained by engineering dispersion and nonlinearity of light propagation in the fiber core or designing a metal-coated tapered fiber tip with a sub-wavelength *** metasurfaces can be assembled on a fiber tip to achieve a large depth of focus and remove *** is also a compact solution to realize the precise delivery of light for in vivo applications,such as deep brain *** optical beam size,shape,and direction could be steered by the probe ***-and nano-technologies integrated with fiber contribute to various approaches to further improve detection limit,sensitivity,optical resolution,imaging depth,and stimulation precision.

关键词： fiber waveguide stimulation

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An in vitro investigation of photodynamic efficacy of FosPeg■ on human colon cancer cells

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Journal of Innovative Optical Health Sciences 2015年第5期8卷 86-93页

作者： Ricky W.K.Wu Ellie S.M.Chu Zheng Huang malini C.olivo David C.W.Ip Christine M.N.Yow Medical Laboratory Science Department of Health Technology&Informatics Hong Kong Polytechnic UniversityHKSARChina Department of Medical Science Tung Wah CollegeHKSARChina College of Photonic&Electronic Engineering Fujian Normal University Fuzhou China University of Colorado Denver Cancer Center COUSA Department of Pharmacy National University of SingaporeSingapore

Photodynamic therapy(PDT)is a novel therapeutic approach for combating various *** involves the administration of a photosensitizer which generates singlet oxygen after light ***■ is the liposomal formulation of *** this in vitro study,the photodynamic efficacy of FosPeg■ on a human colon cancer cell line(HT29)was investigated via studying the cellular uptake of FosPeg■,FosPeg■ PDT mediated photocytotoxicity and the cell death mechanism were ***■ PDT demonstrated its antitumor effect in a drug and light dose-dependent manner in HT-29 *** dose(LD50)was achieved with 0.4g/mL of drug and 3 J/cm^(2) of light ***■ PDT triggered apoptotic cell death via activating caspase cascade and regulating cell cycle *** conclusion,FosPegr-PDT is an effective measure to combat human colon cancer cells.

关键词： FosPeg■ HT-29 apoptosis caspase-3

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