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Papaya Ring Spot Virus:An Understanding of a Severe Positive-Sense Single Stranded RNA Viral Disease and Its Management

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Phyton-International Journal of Experimental Botany 2022年第10期91卷 2099-2110页

作者： Muhammad Umer Mustansar Mubeen Yasir Iftikhar Haider Ali Muhammad Zafar-ul-Hye Rafia Asghar Mazhar Abbas malik abdul rehman Ernesto A.Moya-Elizondo Yuejun He Institute for Forest Resources and Environment of Guizhou and Forestry College Research Centre of Forest EcologyGuizhou UniversityGuiyang550025China Department of Plant Pathology College of AgricultureUniversity of SargodhaSargodha40100Pakistan College of Bioscience University of BirminghamBirminghamB152TTUK Department of Soil Science Faculty of Agricultural Sciences and TechnologyBahauddin Zakariya UniversityMultan60800Pakistan Department of Plant Pathology PMAS University of Arid AgricultureRawalpindi43490Pakistan Department of Agriculture and Agribusiness Management University of Karachi Karachi75270Pakistan Citrus Research Institute Sargodha40100Pakistan Department of Plant Production Faculty of AgronomyUniversidad de ConcepciónChillán3780000Chile

Viral diseases have been studied in-depth for reducing quality,yield,health and longevity of the fruit,to highlight the economic ***-sense single-stranded RNA viruses are more devastating among all viruses that infect fruit *** of the best examples is papaya ringspot virus(PRSV).It belongs to the genus Potyvirus and it is limited to cause diseases on the family Chenopodiaceae,Cucurbitaceae and *** virus has a serious threat to the production of papaya,which is famous for its high nutritional and pharmaceutical *** plant parts such as leaves,latex,seeds,fruits,bark,peel and roots may contain the biological compound that can be isolated and used in pharmaceutical industries as a disease *** disease symptoms consist of vein clearing and yellowing of young *** ring spot patterns with concentric rings and spots on fruit reduce its quality and *** virus has two major strains P and *** former cause disease in papaya and cucurbits while the later one in *** comprises 94.4%protein,including a 36 kDa coat protein which is a component responsible for a non-persistent transmission through aphids,and 5.5%nucleic *** protection,development of transgenic crops,exploring the resistant sources and induction of pathogen derived resistance have been recorded as effective management of *** with these practices reduced aphid population through insecticides and plant extracts have been found ecofriendly approaches to minimize the disease *** of transgenic crops is a big challenge for the success of disease resistant papaya *** aim of this review is to understand the genomic nature of PRSV,detection methods and the different advanced control meth*** review article will be helpful in developing the best management strategies for controlling PRSV.

关键词： Papaya obligate parasite +ssRNA PRSV aphid genomic characterization and functions

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Antagonistic Potential of Bacterial Species against Fungal Plant Pathogens(FPP)and Their Role in Plant Growth Promotion(PGP):A Review

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Phyton-International Journal of Experimental Botany 2022年第9期91卷 1859-1877页

作者： Amjad Ali Yasir Iftikhar Mustansar Mubeen Haider Ali Muhammad Ahmad Zeshan Zohaib Asad Muhammad Zafar-ul-Hye malik abdul rehman Mazhar Abbas Muhammad Rafique Muhammad Usman Ghani Department of Plant Protection Sivas University of Science and TechnologySivas58140Turkey Department of Plant Pathology College of AgricultureUniversity of SargodhaSargodha40100Pakistan College of Bioscience University of BirminghamBirminghamB152TTUK Department of Plant Pathology PMAS University of Arid AgricultureRawalpindi43490Pakistan Department of Soil Science Faculty of Agricultural Sciences and TechnologyBahauddin Zakariya UniversityMultan60800Pakistan Citrus Research Institute Sargodha40100Pakistan Department of Agriculture and Agribusiness Management University of Karachi75270KarachiPakistan Pest warning&Quality Control of Pesticides SahiwalPunjab40210Pakistan Institute of Soil and Environmental Sciences University of AgricultureFaisalabad38000Pakistan

Since the 19th century to date,the fungal pathogens have been involved in causing devastating diseases in *** types of fungal pathogens have been observed in important agricultural crops that lead to significant pre and postharvest *** application of synthetic fungicide against the fungal plant pathogens(FPP)is a traditional management practice but at the same time these fungicides kill other beneficial microbes,insects,animal,and humans and are harmful to *** antagonistic microorganism such as bacteria are being used as an alternate strategy to control the *** antagonistic species are cost-effective and eco-friendly in *** biocontrol bacteria have a broad mechanism against fungal pathogens present in the phyllosphere and rhizosphere of the *** antagonistic bacteria have different strategies against the FPP,by producing siderophore,biofilm,volatile organic compounds(VOCs),through parasitism,antibiosis,competition for limited resources and induce systemic resistance(ISR)in the host plant by activating the immune *** commercial bio-products synthesized by the major bacterial species Pseudomonas syringae,Burkholderia cepacia,Streptomyces griseoviridis,Pseudomonas fluorescens and Bacillus subtilis are used to control Fusarium,Pythium,Rhizoctonia,Penicillium,Alternaria,and *** commercial bio-formulations of bacteria act as both antifungal and plant growth *** Plant growth-promoting rhizobacteria(PGPR)played a significant role in improving plant health by nitrogen-fixing,phosphorus solubilization,phytohormones production,minimizing soil metal contamination,and by ACC deaminase antifungal *** articles are available on the specific antifungal activity of bacteria in plant ***,this review article has summarized the information on biocontrol activity of bacteria against the FPP and the role of PGPR in plant growth *** review also provided a complete picture

关键词： Fungi plant pathogens synthetic fungicide antagonism bio-products PGPR

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The surveillance of arsenic levels in the drinking water of primary schools and the assessment of the potential cancer-related health risks of children in Multan, Pakistan

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Emerging Contaminants 2024年第1期10卷 17-23页

作者： Hidayatullah Mahar abdul rehman Memon Aqsa Ishfaq Suhail Ahmed Soomro Mehran University of Engineering&Technology JamshoroPakistan NFC Institute of Engineering&Technology MultanPakistan

To investigate the levels of arsenic(As)in the groundwater and drinking water of primary schools and their potential impact on human health is main objective of this *** assess risk,the following parameters i.e.,carcinogenic indices(CI),hazardous indexes(HI),carcinogenic risk(CR),and hazardous quotient(HQ)have been measured for both dermal and oral arsenic *** study has been focused on primary schools located in four tehsils of Multan,where water samples were *** levels,with concentrations ranging from 3.31 to 191 mg/l,exceeding the safe limit set by the World Health Organization(WHO)have been detected in 99.9%of the *** results show that arsenic content ranged from 3.31 to 191 mg/l,with 99.9%of the samples exceeding the World Health Organization's safe limit of 10 mg/*** CR and CI exceeded the USEPA limit of 10–6 in all four tehsils ranged from 2.6×10^(-6) to 9.4×10^(-6) and 0.00023 to 0.0009 respectively in both dermal and oral cases.,Multan Sadar(1.51,1.39)and Multan City(1.76,1.71)exhibited HI and HQ values that surpassed the threshold of 1.0,signaling a significant likelihood of chronic and cancer-causing health consequences for individuals who are exposed to polluted water causing *** findings suggest that the groundwater in the Multan district poses a significant health risk to humans due to high levels of arsenic *** contamination is associated with an increased risk of mortality from internal cancers(including liver,kidney,lung,and bladder),as well as a higher HI&CI incidence of skin ***,pregnancy outcomes are affected,with associations found between arsenic exposure and lower birth weight,and infant *** researchers propose that water supply organizations and educational institutions promptly take measures to ensure that the affected areas have access to arsenic-free drinking water for the well-being of students and residents.

关键词： Risk assessment Drinking water contamination Arsenic Primary school Pakistan

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Assessment of plastic degradation by indigenous bacteria from waste disposal sites

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Emerging Contaminants 2024年第3期10卷 111-123页

作者： Farzana Javid Ghadir Ali abdul rehman Rehan Naeem Imran Ali Iffat Naz Department of Microbiology and Biotechnology Faculty of Life SciencesAbasyn University Peshawar CampusPeshawar25000Khyber PakhtunkhwaPakistan Department of Life Sciences School of ScienceUniversity of Management and Technology(UMT)Lahore54700Pakistan Department of Microbiology Kohat University of Science and Technology(KUST)Kohat26000Khyber PakhtunkhwaPakistan Department of Biotechnology and Genetic Engineering Kohat University of Science and Technology(KUST)Kohat26000Khyber PakhtunkhwaPakistan Department of Botany Kohat University of Science and Technology(KUST)Kohat26000Khyber PakhtunkhwaPakistan Department of Biology Deanship of Educational ServicesQassim UniversityBuraydah51452Kingdom of Saudi Arabia

The present study focuses on the isolation of bacterial pathogens from waste disposal sites,and examination of their efficiencies for degrading various *** bacterial isolates such as ***,Corynebacterium spp.,Micrococcus spp.,Azotobacter spp.,Pseudomonas spp.,Staphylococcus spp.,and Bacillus *** *** *** the highest percentage reduction in black plastic weight(47.46%),while Corynebacterium spp.,and *** achieved a maximum reduction of 45.76 and 46.42%for pink plastic *** *** Micrococcus *** notable reductions of 46.43%and 56.60%in white and Canteen Stores Department(CSD)biodegradable plastics,*** density(OD)measurements revealed that bacterial isolates achieved maximum OD values with CSD plastics,emphasizing their proficiency in plastic *** Transform Infrared Spectroscopy(FTIR)confirmed the degradation process,with a decrease in the peak value from 2916 to 2914 cm^(-1) for pink plastic,signifying alkane,and alkene *** new peaks at 1398,1371,and 1361 cm^(-1) in the FTIR spectrum of CSD plastic indicated presence of *** Electron Microscopic(SEM)micrographs illustrated bacterial colonization,cracks,and spots on plastic surfaces,while Xray Diffraction(XRD)spectra showed peaks at 4.083,3.705,3.020,and 1.909,suggesting plastic *** conclusion,soil of waste disposal site harbored diverse microbial species,and have highlighted their effectiveness in mitigating environmental pollution by degrading hazardous plastic waste.

关键词： Plastic degradation Bacterial isolates Waste disposal sites Fourier transform infrared spectroscopy Scanning electron microscopy X-ray diffraction Environmental pollution

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Retroperitoneal bronchogenic cyst:A case report and review of literature

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World Journal of Clinical Cases 2024年第15期12卷 2586-2596页

作者： abdul malik Qais Ahmad Naseer Muhammad Asad Iqbal Shi-Ya Han Sheng-Chun Dang Department of General Surgery The Affiliated Hospital of Jiangsu UniversityZhenjiang 212001Jiangsu ProvinceChina Department of Preventive Medicine and Public Health Laboratory Science School of MedicineJiangsu UniversityZhenjiang 212013Jiangsu ProvinceChina Department of Laboratory Medicine School of MedicineJiangsu UniversityZhenjiang 212013Jiangsu ProvinceChina Department of Clinical Pharmacy Shenyang Pharmaceutical UniversityShenyang 110000Liaoning ProvinceChina Department of Surgery Siyang HospitalSuqian 223700Jiangsu ProvinceChina

BACKGROUND Bronchogenic cysts are rare developmental anomalies that belong to the category of congenital enterogenous *** arise from lung buds and are present at *** embryonic foregut is their ***,they are located within the chest cavity,particularly in the cavum mediastinale of the thoracic cavity or lodged in the pulmonary parenchyma,and are considered a type of lung bud *** SUMMARY A 49-year-old male patient was admitted to the hospital due to the detection of a retroperitoneal mass during a physical *** weeks before admission,the patient underwent a physical examination and routine laboratory tests,which revealed a space-occupying mass in the retroperitoneal *** patient did not report any symptoms(such as abdominal pain,flatulence,nausea,vomiting,high fever,or chills).The computed tomography(CT)revealed a retroperitoneal spaceoccupying lesion with minimal enhancement and a CT value of approximately 36 Hounsfield *** lesion was not delineated from the boundary of the pancreatic body and was closely related to the retroperitoneum *** Following a series of tests,an abdominal mass was identified,prompting the implementation of a laparoscopic retroperitoneal mass excision *** the investigation,an 8 cm×7 cm cystic round-shaped mass with a distinct demarcation was identified in the upper posterior region of the ***,full resection of the mass was *** pathological examination reveled a cystic mass characterized by a smooth inner *** cystic mass was found to contain a white,viscous liquid within its capsule.

关键词： Bronchogenic cyst Retroperitoneal space Excision Laparoscopic surgery Case report

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The effects of selenium on the translation efficiency of selenoproteins Gpx1， Gpx4， and Txnrd1 in oxLDL-induced ***926 cells

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Food Science and Human Wellness 2024年

作者： Jiaqi Lu Jing Meng Yanzhi Lang abdul rehman Yamei Yan Linwu Ran School of Public Health， Ningxia Medical University Key Laboratory of Environmental Factors and Chronic Disease Control Yinchuan School of Clinical Medicine， Ningxia Medical University Goji Berry Research Institute， Ningxia Academy of Agriculture and Forestry Sciences

Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins. Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress. This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in ***926 cells induced by oxLDL. The impact of selenium on the viability of ***926 cells was detected by the MTT method， and intracellular ROS level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1， respectively. RNA-seq， quantitative real-time PCR (RT-qPCR)， and western blot were used to investigate the selenoprotein expression. Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling (Ribo-Seq) coupled with transcriptomics. Our data showed that selenium supplementation (0.5 μmol/L) significantly decreased ROS production， reduced mitochondrial inner membrane potential and increased the proliferative activity of ***926 cells induced by oxLDL. Moreover， The protective effects of selenium against oxLDL-induced ***926 cell injury were associated with the upregulation of the expressions of selenoproteins GPX1， GPX4， and TXNRD1. Furthermore， the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled， but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL. Finally， Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1， GPX4， and TXNRD1 were regulated at the translation process level. These findings suggested that selenium could have preventive effects in oxLDL induced ***926 cell injury by regulating the selenoprotein expression， and the selenoproteins expressions at the translation level in vascular endothelial cells need further study.

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The Impact of Solid Waste Generation, Storage and Separation Practices among Households on the Environment in Freetown

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Journal of Geoscience and Environment Protection 2024年第8期12卷 219-238页

作者： abdul malik Bangura Patrick Fayia Kanty College of Environmental Science and Engineering Tongji University Shanghai China

As urbanization and population growth continue to increase in Freetown, due to changes in economic, social, environmental, political, and demographic factors, the municipal solid waste (MSW) generation also continues to increase, making its management difficult for the municipal authority. Efficient separation and storage of solid waste at the source of generation can boost resource and energy recovery from MSW. This study examines the municipal solid waste management (MSWM) process, focusing on generation, storage and separation practices among households and their impact on the environment in Freetown. It emphasizes the inclusion of MSWM programs in primary schools to raise public awareness, the implementation of effective waste management practices, and the enforcement of related policies to enhance the MSWM sector, contributing to sustainable MSWM in Freetown. By utilizing both qualitative and quantitative methods, 393 structured questionnaires were administered across three selected sections to collect data on household solid waste storage and separation practices. The analysis employed descriptive statistics, using Origin-Pro9 and MS Excel. The findings show that with a population of 1.53 million people in Freetown, the per capita solid waste generation is 0.58 kg per day. The findings also show that 97% of the households have storage facilities as a result of the increase in awareness and education about the proper storage of solid waste. However, 96% of respondents do not practice separation of solid waste at the source of generation, which has become a concern among researchers in Sierra Leone. Additionally, 88% of respondents are unaware of ISWM principles, with only 12% aware, most of whom have received some education on proper solid waste management. The study recommends improving MSWM in Freetown to protect public health and the environment.

关键词： Generation Storage Separation Household Municipal Solid Waste Freetown

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Cardioembolic Stroke as a Sequela of General Ventricular Hypokinesia Secondary to Acute Myocarditis in a 16-Year-Old Male

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Open Journal of Emergency Medicine 2024年第3期12卷 85-94页

作者： abdul Khattan rehman Renaldo Pavrey Nazir Juvale Murtaza Bagwala Department of Emergency Medicine Saifee Hospital Mumbai India Department of Emergency Medicine Nanavati Max Super Specialty Hospital Mumbai India Department of Cardiology Saifee Hospital Mumbai India

Paediatric cardiac disease is an established cause of ischemic stroke in the neonatal and infantile groups. These diseases may be congenital or acquired. However, clinical myocarditis is in itself, relatively uncommon in older children. The most common pathogen is Coxsackie virus B. The offending agent instigates an immune response, which causes myocardial oedema with eventual systolic and diastolic dysfunction. Cardioembolic stroke can occur secondary to an intra-mural thrombus in a dysfunctional atrium or ventricle. We describe the case of an adolescent male with acute myocarditis complicated by a thromboembolic stroke. After initial management of acute pulmonary oedema and heart failure with restricted ejection fraction (HFrEF), the child developed seizure-like symptoms on the 10th day of hospitalization, prompting urgent neuro-radio diagnosis, which revealed acute infarcts in the cerebellum and frontal lobe of the cerebrum. We believe this case to be of clinical relevance because;1) The diagnosis of an acute stroke in children is often delayed due to the atypical clinical presentation and often the absence of traditional stroke-like symptoms, and 2) There is a lack of sufficient high-quality evidence regarding the predictors and the immediate management of stroke in paediatric heart disease, as well as inadequate data on prevalence and incidence in paediatric cardioembolic strokes.

关键词： Paediatric Ischemic Stroke Adolescent Myocarditis Ventricular Hypokinesia Cardioembolic

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Effect of Light Emitting Diodes (LEDs) on Growth, Mineral Composition, and Nutritional Value of Wheat & Lentil Sprouts

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1117-1128页

作者： abdul Momin Amana Khatoon Wajahat Khan Dilsat Bozdoğan Konuşkan Muhammad Mudasar Aslam Muhammad Jamil Shafiq Ur rehman Baber Ali Alevcan Kaplan Sana Wahab Muhammad Nauman Khan Sezai Ercisli Mohammad Khalid Al-Sadoon Department of Botany Kohat University of Science&TechnologyKohat26000Pakistan Department of Food Engineering Faculty of AgricultureHatay Mustafa Kemal UniversityHatay31000Türkiye Department of Botany University of Science&TechnologyBannu28100Pakistan Department of Biotechnology and Genetic Engineering Kohat University of Science&TechnologyKohat26000Pakistan Department of Biology University of HaripurKhyber Pakhtunkhwa22620Pakistan School of Science Western Sydney UniversityPenrithNew South Wales2151Australia Department of Crop and Animal Production Sason Vocational SchoolBatman UniversityBatman72060Türkiye Department of Plant Sciences Quaid-i-Azam UniversityIslamabad45320Pakistan Department of Botany Islamia College PeshawarPeshawar25120Pakistan Department of Horticulture Agricultural FacultyAtaturk UniversityErzurum25240Türkiye HGF Agro Ata TeknokentErzurumTR-25240Türkiye Department of Zoology College of ScienceKing Saud UniversityRiyadh11451Saudi Arabia

Sprouts are ready-to-eat and are recognized worldwide as functional components of the human *** advances in innovative agricultural techniques could enable an increase in the production of healthy *** use of light-emitting diode(LED)in indoor agricultural production could alter the biological feedback loop,increasing the functional beneﬁts of plant foods such as wheat and lentil sprouts and promoting the bioavailability of *** effects of white(W),red(R),and blue(B)light were investigated on the growth parameters and nutritional value of wheat and lentil *** the laboratory,seeds were sown under three different LED treat-ments:white,red,and blue light,while normal incandescent light served as a *** seed germina-tion improved by 18.34%and 12.67%for wheat and 18.34%and 12.67%for lentil sprouts under LED treatments R and B,*** increase in total soluble protein and sugar by 33.4%and 9.23%in wheat and by 31.5%and 5.87%in lentils was observed under the R LED *** C concentrations in wheat and lentils were signiﬁcantly increased by R LED compared to all other *** parameters,including potassium and sodium concentrations,were signiﬁcantly increased under red and blue light compared to the control;white light,on the other hand,signiﬁcantly decreased all these *** to the experimental data,red and blue LED light could be beneﬁcial in the production of functional wheat and lentil sprouts with high nutrient concentrations.

关键词： Growth parameters LED nutritional value sprouts total soluble proteins

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Left bundle branch pacing vs biventricular pacing in heart failure patients with left bundle branch block:A systematic review and meta-analysis

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World Journal of Cardiology 2024年第1期16卷 40-48页

作者： Farah Yasmin abdul Moeed Rohan Kumar Ochani Hamna Raheel malik Ali Ehtsham Awan Ayesha Liaquat Arisha Saleem Muhammad Aamir Nael Hawwa Salim Surani Department of Internal Medicine Yale University School of MedicineNew HavenCT 06511United States Department of Medicine Dow University of Health ScienceKarachi 74200SindhPakistan Department of Medicine SUNNY Upstate Medical UniversitySyracuseNY 13210United States Department of Cardiovascular Medicine Lehigh Valley Heart and Vascular InstituteAllentownPA 18105United States Department of Medicine Cleveland ClinicClevelandOH 44195United States Department of Medicine&Pharmacology Texas A&M UniversityCollege StationTX 77843United States

BACKGROUND Left bundle branch pacing(LBBP)is a novel pacing modality of cardiac resynchronization therapy(CRT)that achieves more physiologic native ventricular activation than biventricular pacing(BiVP).AIM To explore the validity of electromechanical resynchronization,clinical and echocardiographic response of *** Systematic review and Meta-analysis were conducted in accordance with the standard guidelines as mentioned in detail in the methodology *** In our analysis,the success rate of LBBP-CRT was determined to be 91.1%.LBBP CRT significantly shortened QRS duration,with significant improvement in echocardiographic parameters,including left ventricular ejection fraction,left ventricular end-diastolic diameter and left ventricular end-systolic diameter in comparison with *** A significant reduction in New York Heart Association class and B-type natriuretic peptide levels was also observed in the LBBP-CRT group vs BiVP-CRT ***,the LBBP-CRT cohort had a reduced pacing threshold at follow-up as compared to BiVP-CRT.

关键词： Left bundle branch pacing Biventricular pacing QRS duration Left ventricular ejection fraction Heart failure

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