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Image Processing of Biological Liquids Films for Medical Diagnostics

Journal of Electronic Science and Technology 2020年第1期18卷 19-30页

作者： maksim aleksandrovich baranov Institute of Physics Nanotechnology and TelecommunicationsPeter the Great St.Petersburg Polytechnic UniversitySt.Petersburg 195251

In this paper, the development of smart medical autonomous technology is considered. An example of a smart medical autonomous distributed system for diagnostics is also discussed. To develop this system for medical image analysis we review several processing methods. The use of the cuneiform dehydration method for medical diagnosis is considered. The experimental results obtained for blood serum dehydrated films are presented. The author proposes an algorithm for the primary identification of structures formed in the films and their use for automated detection of various structures for diagnostic purposes. The paper describes the first stage of image processing, i.e. the selection of filtering types for the correct identification of structural features and characteristics of the images. The results of filtering and some computational results of various types of structures in the films are presented.

关键词： Biological liquids dehydrated films image processing medical diagnostic

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Role of Electromagnetic Fluctuations in Organic Electronics

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Journal of Electronic Science and Technology 2020年第1期18卷 52-58页

作者： Vladimir M.Mostepanenko Elena N.Velichko maksim aleksandrovich baranov Institute of Physics Nanotechnology and TelecommunicationsPeter the Great St.Petersburg Polytechnic UniversitySt.Petersburg 195251 Central Astronomical Observatory at Pulkovo of Russian Academy of Sciences St.Petersburg 196140

Thin organic films find expanding applications in electronic and optoelectronic devices, biotechnology,food packing, and for many other purposes. Among other factors, the stability of films with a thickness below a micrometer is determined by the zero-point and thermal fluctuations of the electromagnetic field. These fluctuations result in the van der Waals and Casimir free energy and forces between a film and a substrate. The fluctuationinduced force may be both attractive and repulsive making the film either more or less stable, respectively. Here, we review recently obtained results for the Casimir free energy of both freestanding and deposited on the metallic and dielectric substrates peptide films. We also perform computations for the free energy of the peptide films deposited on a silica glass substrate in the region of parameters where this free energy vanishes. Possible applications of the obtained results are discussed.

关键词： Casimir free energy organic electronics peptide film

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Molecular and cellular changes in the post-traumatic spinal cord remodeling after autoinfusion of a genetically-enriched leucoconcentrate in a mini-pig model

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Neural Regeneration Research 2023年第7期18卷 1505-1511页

作者： Maria Aleksandrovna Davleeva Ravil Rasimovich Garifulin Farid Vagizovich Bashirov Andrei aleksandrovich Izmailov Leniz Faritovich Nurullin Ilnur Ildusovich Salafutdinov Dilara Zilbarovna Gatina Dmitrij Nikolaevich Shcherbinin Andrei aleksandrovich Lysenko Irina Leonidovna Tutykhina maksim Mikhailovich Shmarov Rustem Robertovich Islamov Department of Histology Cytology and EmbryologyKazan State Medical UniversityKazanRussia Кazan Institute of Biochemistry and Biophysics Federal Research Center of Kazan Scientific Center of Russian Academy of SciencesKazanRussia Institute of Fundamental Medicine and Biology Kazan Federal UniversityKazanRussia The National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation MoscowRussia

Post-traumatic spinal cord remodeling includes both degenerating and regenerating processes,which affect the potency of the functional recovery after spinal cord injury(SCI).Gene therapy for spinal cord injury is proposed as a promising therapeutic strategy to induce positive changes in remodeling of the affected neural *** our previous studies for delivering the therapeutic genes at the site of spinal cord injury,we developed a new approach using an autologous leucoconcentrate transduced ex vivo with chimeric adenoviruses(Ad5/35)carrying recombinant *** the present study,the efficacy of the intravenous infusion of an autologous genetically-enriched leucoconcentrate simultaneously producing recombinant vascular endothelial growth factor(VEGF),glial cell line-derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)was evaluated with regard to the molecular and cellular changes in remodeling of the spinal cord tissue at the site of damage in a model of mini-pigs with moderate spinal cord *** animals were randomly divided into two groups of 4 pigs each:the therapeutic(infused with the leucoconcentrate simultaneously transduced with a combination of the three chimeric adenoviral vectors Ad5/35‐VEGF165,Ad5/35‐GDNF,and Ad5/35‐NCAM1)and control groups(infused with intact leucoconcentrate).The morphometric and immunofluorescence analysis of the spinal cord regeneration in the rostral and caudal segments according to the epicenter of the injury in the treated animals compared to the control mini-pigs showed:(1)higher sparing of the grey matter and increased survivability of the spinal cord cells(lower number of Caspase-3-positive cells and decreased expression of Hsp27);(2)recovery of synaptophysin expression;(3)prevention of astrogliosis(lower area of glial fibrillary acidic protein-positive astrocytes and ionized calcium binding adaptor molecule 1-positive microglial cells);(4)higher growth rates of regeneratingβIII-tubulin-positi

关键词： autologous genetically-enriched leucoconcentrate chimeric adenoviral vector gene therapy glial cell line-derived neurotrophic factor mini-pig neural cell adhesion molecule spinal cord contusion injury vascular endothelial growth factor

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