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Disruption of colonic barrier function and induction of mediator release by strains of Campylobacter jejuni that invade epithelial cells

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World Journal of Gastroenterology 2008年第48期14卷 7345-7352页

作者： Johannes Beltinger Jo del Buono maeve m skelly John Thornley Robin C Spiller William A Stack Christopher J Hawkey Wolfson Digestive Diseases Centre Nottingham University Hospital QMC Campus

AIm: To study the mechanisms by which Campylobacter jejuni (C. jejuni) causes inflammation and diarrhea. In particular, direct interactions with intestinal epithelial cells and effects on barrier function are poorly under- stood. mETHODS: To model the initial pathogenic effects of C. jejuni on intestinal epithelium, polarized human colonic HCA-7 monolayers were grown on permeabilized filters and infected apically with clinical isolates of C. jejuni. Integrity of the monolayer was monitored by changes in monolayer resistance, release of lactate dehydrogenase, mannitol fluxes and electron microscopy. Invasion of HCA-7 cells was assessed by a modified gentamicin protection assay, translocation by counting colony forming units in the basal chamber, stimulation of mediator release by immunoassays and secretory responses in monolayers stimulated by bradykinin in an Ussing chamber. RESULTS: All strains translocated across monolayers but only a minority invaded HCA-7 cells. Strains that invaded HCA-7 cells destroyed monolayer resistance over 6 h, accompanied by increased release of lactate dehydrogenase, a four-fold increase in permeability to [3H] mannitol, and ultrastructural disruption of tight junctions, with rounding and lifting of cells off the filter membrane. Synthesis of interleukin (IL)-8 and prostaglandin E2 was increased with strains that invaded the monolayer but not with those that did not. CONCLUSION: These data demonstrate two distinct effects of C. jejuni on colonic epithelial cells and provide an informative model for further investigation of initial host cell responses to C. jejuni.

关键词： Campylobacter jejuni Cell invasion Cellculture Chloride secretion Colonocyte HCA-7 cells membrane permeability monolayer mucosal barrier Ussing chamber

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神经损伤与功能重建 2024年第8期19卷 F0003-F0003页

作者： Adina N macmahon Copas Sarah F mcComish Andreea Petrasca Rachel mcCormack Daniel Ivers Anna Stricker Jean m Fletcher maeve A Caldwell 唐颖馨 Discipline of Physiology School of MedicineTrinity Biomedical Sciences InstituteTrinity College DublinDublinIreland Trinity College Institute for Neuroscience Trinity CollegeDublinIreland School of Biochemistry and Immunology Trinity Biomedical Sciences InstituteTrinity College DublinDublinIreland School of medicine Trinity Biomedical Sciences InstituteTrinity College DublinDublinIreland 不详

星形胶质细胞是维持稳态的介质,但在帕金森病(Parkinson’s disease,PD)中参与神经炎症。越来越多的证据表明外周免疫细胞参与了PD的发病机制。因此,本研究旨在确定外周免疫细胞分泌的细胞因子在调节中脑星形胶质细胞反应性中的潜在作... 详细信息

星形胶质细胞是维持稳态的介质,但在帕金森病(Parkinson’s disease,PD)中参与神经炎症。越来越多的证据表明外周免疫细胞参与了PD的发病机制。因此,本研究旨在确定外周免疫细胞分泌的细胞因子在调节中脑星形胶质细胞反应性中的潜在作用。人类诱导性多能干细胞(iPSC)衍生的中脑星形胶质细胞暴露于5%和10%的CD4^(+)T细胞条件培养基(CD4Cm)24小时、72小时和7天,以评估长期暴露的影响。此外,星形胶质细胞还暴露于Th17细胞细胞因子IL-17A(10 ng/mL),单独或与TNF-α(0.3 ng/mL)结合,在24小时、72小时和7天评估2种细胞因子可能的协同效应。CD4Cm在中脑星形胶质细胞中引起了急性及长期的变化。观察到了NFκB向细胞核的转移增加,以及在24小时时促炎基因IL-1β和CXCL10的表达增加;在24小时和48小时时C3、LCN2和IL-6的表达增加;同时在这些时间点促炎细胞因子IL-6和CXCL10的释放也有所增加。IL-17A和TNF-α的组合对增加促炎基因表达和细胞因子释放产生了协同效应。IL-17A和TNF-α在24小时时增加了S100β的强度,在72小时时减少了细胞核面积并增加了星形胶质细胞的圆度。在72小时时观察到了γH2AX强度的协同效应,并且在7天时观察到了乳酸脱氢酶(LDH)释放的增加。我们的结果表明,IL-17A和TNF-α协同作用,增强了中脑星形胶质细胞的反应性,其程度类似于CD4Cm。这突出了外周免疫细胞分泌的细胞因子在增强中脑星形胶质细胞反应状态方面的重要性,并提示了它们在PD发病机制中的可能作用。

关键词：帕金森病星形胶质细胞细胞因子诱导性多能干细胞神经炎症反应性星形胶质细胞

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management information systems for the information age / 6th ed.

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2007年

作者： Stephen Haag maeve Cummings Amy Phillips.

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Assessment of Public Health Risks Associated with Naphthalene Entering Residences and Commercial Space from Vapor Intrusion at mGP Sites

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Journal of Environmental Protection 2013年第12期4卷 11-17页

作者： Robin DeHate Brian skelly marie Bourgeois Giffe Johnson Raymond Harbison 1Center for Environmental/Occupational Risk Analysis and management College of Public Health University of South Florida Tampa USA 2GEI Consultants Inc. Valrico USA. Center for Environmental/Occupational Risk Analysis and management College of Public Health University of South Florida Tampa USA GEI Consultants Inc. Valrico USA.

Naphthalene, a constituent of coal tar, is a contaminant frequently found at former manufactured gas plants (mGP). Development at these sites has resulted in residential and commercial areas with potential exposures from vapor intrusion adversely affecting indoor air of residences and buildings. Naphthalene is routinely analyzed in soil vapor intrusion assessments for properties overlying and surrounding former mGP sites. The United States Environmental Protection Agency (EPA) has a proposed unit risk factor and the State of California has promulgated a unit risk factor for naphthalene. Naphthalene exposure from vapor intrusion is potentially a public health risk. The purpose of this study was to evaluate three sites located in the northeast United States to determine the frequency of naphthalene detection in indoor air. A total of 79 properties were included in the study. A total of 546 indoor air samples were analyzed for naphthalene on 161 occasions. Naphthalene concentrations ranged from 0.26 to 51 μg/m3 of air. Only 3 of the 546 indoor air samples detected naphthalene above the ninety-fifth percentile background value of 12 μg/m3 of air. Risk analysis indicated naphthalene vapor intrusion was not a public health risk among the 79 properties built on or near the former mGP sites.

关键词： Soil Vapor Intrusion Naphthalene Volatile Organic Compounds Risk Assessment manufactured Gas Plant

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management information systems for the information age / Stephen Haag, maeve Cummings, Donald J. mcCubbrey. 5th ed.

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2005年

作者： Stephen Haag maeve Cummings Donald J. mcCubbrey.

Includes bibliographical references and index.

ISBN: (数字)0072935863

ISBN: (纸本)0072935863

Includes bibliographical references and index.

关键词： management information systems.

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Body mass index estimation and measurement by healthcare professionals

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Open Journal of Preventive medicine 2012年第3期2卷 265-271页

作者： Tomás Ahern Kirsten Doherty Daniel Kapeluto maeve Davis Una mulholland Edwina Rossiter Irene Gilroy Astrid Billfalk-Kelly Patricia FitzPatrick Leslie Daly Cecily Kelleher Donal O’Shea Department of Endocrinology St Vincent’s University Hospital Elm Park Dublin 4 Ireland Department of Family medicine Memorial University of Newfoundland St. John’s Newfoundland and Labrador Canada Department of Preventive medicine and Health Promotion St Vincent’s University Hospital Elm Park Dublin 4 Ireland School of Health & Science Department of Nursing Midwifery and Health Studies Dundalk Institute of Technology Dublin Road Dundalk Co Louth Ireland

Background: Severe obesity has increased more than three-fold in prevalence over the past fifteen years in Europe and the United States. Correctly identifying severe obesity permits access to mortality-reducing interventions. We aimed to determine the accuracy of healthcare professionals (HCPs) in recognising severe obesity and the frequency of body mass index (BmI) assessment by HCPs. methods: We performed two cross-sectional surveys: one of 206 HCPs in Ireland and Canada and another of 515 Irish hospital healthcare records. The first survey evaluated BmI estimation from photographs and the second examined recording of weight and height during outpatient clinic visits. Results: HCPs underestimated the BmI of severely obese people by an average of 22% to 39%. For a patient with a BmI of 52 kg/m2, 35.4% of family physicians appreciated that the patient was severely obese (BmI > 40 kg/m2) compared with 81.0% of endocrinologists. During clinic visits only 18.1% (n = 75), 1% (n = 4) and 0% (n = 0) of patients had their respective weights, heights and BmIs recorded. Conclusions: HCPs frequently fail to recognise severe obesity from photographs and, in our centre, do not routinely record weight or height. Whether patient outcomes can be improved by measuring weight and height during every HCP encounter warrants further study.

关键词： Severe Obesity Body mass Index Healthcare Professionals

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《翻译研究语料库入门》述评

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当代语言学 2007年第1期9卷 87-91页

作者： m．Olohan 黄勤（述评）不详华中科技大学外国语学院湖北武昌430074

《翻译研究语料库入门》（Introducing Corpora in Translation Studies）一书由Routledge公司于2004年出版.作者maeve Olohan是英国曼彻斯特大学翻译与跨文化中心的高级讲师, 曾与mona Baker 一起创建了世界上第一个翻译语料库, 在翻... 详细信息

《翻译研究语料库入门》（Introducing Corpora in Translation Studies）一书由Routledge公司于2004年出版.作者maeve Olohan是英国曼彻斯特大学翻译与跨文化中心的高级讲师, 曾与mona Baker 一起创建了世界上第一个翻译语料库, 在翻译语料库研究方面颇有建树.该书是她从事翻译语料库研究的一个总结.本书除绪论和结论外,共分十章.重点探讨翻译研究语料库的应用,并为熟悉翻译研究但缺乏语料库知识的研究者介绍语料库方面的知识.

关键词：语料库研究翻译研究述评曼彻斯特大学 ge公司文化中心研究者知识

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急性脊髓损伤患者治疗的临床操作指南:关于mRI基线在临床治疗决策与结果预测中作用的几点建议（英文）

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中华神经外科疾病研究杂志 2017年第6期16卷 529-529页

作者： Fehlings mG martin AR Tetreault LA Aarabi B Anderson P Arnold Pm Brodke D Burns AS Chiba K Dettori JR Furlan JC Hawryluk G Holly LT Howley S Jeji T Kalsi-Ryan S Kotter m Kurpad S Kwon BK marino RJ massicotte E merli G middleton JW Nakashima H Nagoshi N Palmieri K Singh A skelly AC Tsai EC Vaccaro A Wilson JR Yee A Harrop JS Toronto Western Hospital University Health Network University of Toronto University College Cork University of maryland University of Wisconsin University of Kansas medical Center The University of Kansas University of Utah Toronto Rehabilitation Institute University Health Network National Defense medical College Spectrum Research Inc University of California at Los Angeles Christopher & Dana Reeve Foundation Ontario Neurotrauma Foundation University of Cambridge medical College of Wisconsin University of British Columbia Thomas Jefferson University Hospital University of Sydney Nagoya University Keio University The University of Kansas Ottawa Hospital Research Institute Thomas Jefferson University Li Ka Shing Knowledge Institute St. Michael's Hospital Sunnybrook Health Sciences Centre

The objective of this guideline is to outline the role of magnetic resonance imaging(mRI) in clinical decision making and outcome prediction in patients with traumatic spinal cord injury(SCI).methods A systematic review of the literature was conducted to address key questions related to the use of mRI in patients with traumatic *** review focused on longitudinal studies that controlled for baseline neurologic status.A multidisciplinary Guideline Development Group(GDG) used this information,their clinical expertise,and patient input to develop recommendations on the use of mRI for SCI *** on GRADE(Grading of Recommendation,Assessment,Development and Evaluation),a strong recommendation is worded as " we recommend," whereas a weaker recommendation is indicated by "we suggest." Results Based on the limited available evidence and the clinical expertise of the GDG,our recommendations were:(1) "We suggest that mRI be performed in adult patients with acute SCI prior to surgical intervention,when feasible,to facilitate improved clinical decision-making"(quality of evidence,very low) and(2) "We suggest that mRI should be performed in adult patients in the acute period following SCI,before or after surgical intervention,to improve prediction of neurologic outcome "(quality of evidence,low).Conclusions These guidelines should be implemented into clinical practice to improve outcomes and prognostication for patients with SCI.

关键词：神经外科疾病医学研究患者

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