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Exposure to cuticular bacteria can alter host behavior in a funnel-weaving spider

Current Zoology 2018年第3期64卷 721-726页

作者： Olivia B.PARKS Krishna S.KOTHAMASU Michael J.ZIEMBA Morgan BENNER madison cristinziano Serena KANTZ Daniel LEGER John LI Devanshi PATEL William RABUSE Samantha SUTTON Amandi WILSON Priyanka BAIREDDY Aditi A.KAMAT Mariah J.CALLAS Matthew J.BORGES Marysa N.SCALIA Emily KLENK Gabrielle SCHERER Maria M.MARTINEZ Sarah R.GRUBS Nancy KAUFMANN Jonathan N.PRUITT Carl N.KEISER Department of Biological Sciences University of PittsburghPittsburghPA 15260USA Department of Ecology Evolutionand Marine BiologyUniversity of CaliforniaSanta BarbaraCA 93106USA Rice University Academy of Fellows Rice UniversityHoustonTX 77005USA Department of BioSciences Rice UniversityHoustonTX 77005USA

Contact with environmental microbes are arguably the most common species interaction in which any animal *** have noted diverse relationships between hosts and resident microbes,which can have strong consequences for host development,physiology,and behavior. Many of these studies focus specifically on pathogens or beneficial microbes,while the benign microbes,of which the majority of bacteria could be described,are often ***,we explore the nature of the relationships between the grass spider Agelenopsis pennsylvanica and bacteria collected from their cuticles in ***,using culture-based methods,we identified a portion of the cuticular bacterial communities that are naturally associated with these ***,we topically exposed spiders to a subset of these bacterial monocultures to estimate how bacterial exposure may alter 3 host behavioral traits:boldness,aggressiveness,and activity *** conducted these behavioral assays 3 times before and 3 times after topical application,and compared the changes observed in each trait with spiders that were exposed to a sterile control *** identified 9 species of bacteria from the cuticles of 36 spiders and exposed groups of 20 spiders to 1 of 4 species of cuticular *** found that exposure to Dermacoccus nishinomiyaensis and Staphylococcus saprophyticus was associated with a lO-fold decrease in the foraging aggressiveness of spiders toward prey in their *** bacterial exposure did not have survival consequences for hosts,these data suggest that interactions with cuticular bacteria,even non- pathogenic bacteria,could alter host behavior.

关键词： Agelenopsis pennsylvanica aggressiveness Araneae cuticular bacteria personality

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Prader-Willi Syndrome and the Use of Medical Nutrition Therapy

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Case Reports in Clinical Medicine 2024年第8期13卷 339-344页

作者： Alexis Howarth Cameron Carroll madison Battersby Stephen Brown JuliSu DiMucci-Ward Department of Nutrition Edward Via College of Osteopathic Medicine Spartanburg South Carolina USA

Title: Integrating Consistent Individualized Carbohydrate-Controlled Anti- Inflammatory Nutritional Plan (C-ICAN) in the Management of Prader-Willi Syndrome: A Case Report. Prader–Willi syndrome (PWS) is a rare genetic disorder caused by a loss of function of specific genes on chromosome 15. Patients with this disease present unique challenges in management, particularly regarding obesity and nutritional regulation as the disease symptoms change depending on the age of the patient and the phase of the disease. These challenges pose critical stressors to caregivers and their families. We present a case report of a 5-year-old Caucasian male diagnosed with PWS, exhibiting failure to thrive and uncontrolled weight gain. His caregiver was his elderly grandmother who, by her own admission, was ill-equipped to deal with the patient’s physical symptoms and his behavior in response to dietary restrictions. Through a multidisciplinary approach involving medical nutrition therapy (MNT) involving the implementation of a Consistent Individualized Carbohydrate-Controlled Anti-Inflammatory Nutritional plan (C-ICAN), growth hormone supplementation, and behavioral interventions patient markedly improved physically and emotionally.

关键词： Prader-Willi Syndrome Growth Hormone Medical Nutrition Therapy Eating Control Obesity

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Pelvic fractures in blunt trauma patients:A comparative study

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World Journal of Orthopedics 2024年第5期15卷 418-434页

作者： Alexander A Fokin Joanna Wycech Knight madison E Tharp Kyler C Brinton Phoebe K Gallagher Justin Fengyuan Xie Russell D Weisz Ivan Puente Trauma and Critical Care Services Delray Medical CenterDelray BeachFL 33484United States Charles E Schmidt College of Medicine Department of SurgeryFlorida Atlantic UniversityBoca RatonFL 33431United States Trauma and Critical Care Services Broward Health Medical CenterFort LauderdaleFL 33316United States Charles E Schmidt College of Medicine Florida Atlantic UniversityBoca RatonFL 33431United States Herbert Wertheim College of Medicine Department of SurgeryFlorida International UniversityMiamiFL 33199United States

BACKGROUND Pelvic fractures(PF)with concomitant injuries are on the rise due to an increase of high-energy *** of the elderly population with age related comorbidities further complicates the *** organ injuries are kindred with PF due to the proximity to pelvic *** of contrast blush(CB)on computed tomography in patients with PF is considered a sign of active bleeding,however,its clinical significance and association with outcomes is *** To analyze polytrauma patients with PF with a focus on the geriatric population,co-injuries and the value of contrast *** This retrospective cohort study included 558 patients with PF admitted to level 1 trauma center(01/2017-01/2023).Analyzed variables included:Age,sex,mechanism of injury(MOI),injury severity score(ISS),Glasgow coma scale(GCS),abbreviated injury scale(AIS),co-injuries,transfusion requirements,pelvic angiography,embolization,laparotomy,orthopedic pelvic surgery,intensive care unit and hospital lengths of stay,discharge disposition and *** study compared geriatric and non-geriatric patients,patients with and without CB and abdominal *** score matching was implemented in comparison *** PF comprised 4%of all trauma admissions.89 patients had CB.286(52%)patients had concomitant injuries including 93(17%)patients with abdominal *** patients compared to non-geriatric had more falls as MOI,lower ISS and AIS pelvis,higher GCS,less abdominal co-injuries,similar CB and angio-embolization rates,less orthopedic pelvic surgeries,shorter lengths of stay and higher *** propensity matching,orthopedic pelvic surgery rates remained lower(8%vs 19%,P<0.001),hospital length of stay shorter,and mortality higher(13%vs 4%,P<0.001)in geriatric *** of 89 patients with CB,45(51%)were *** propensity matching,patients with CB compared to without CB had more pelvic angiography(71%vs 12%,P<0.001),hi

关键词： Pelvic fractures Geriatric trauma patients Abdominal co-injuries Contrast blush Contrast extravasation Angioembolization Polytrauma patients Surgical interventions Blunt trauma External and internal fixation for pelvic stabilization

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礼来闯入减肥药市

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商业周刊（中文版） 2024年第3期 62-67页

作者： madison Muller Baptiste Virot(图) 王湛(译) 不详

礼来公司在减肥药领域起步较晚但现如今,其新药Zepbound有望成为史上最畅销的药物,如果礼来公司(EliLilly&Co.)听取了理查德·迪马奇(Richard Di Marchi)的建议,它可能会率先进入价值800亿美元的减肥药市场。30年前,迪马奇在这家总部位... 详细信息

礼来公司在减肥药领域起步较晚但现如今,其新药Zepbound有望成为史上最畅销的药物,如果礼来公司(EliLilly&Co.)听取了理查德·迪马奇(Richard Di Marchi)的建议,它可能会率先进入价值800亿美元的减肥药市场。30年前,迪马奇在这家总部位于印第安纳波利斯的制药公司担任科学家,研究一种名为胰高血糖素样肽-1(GLP-1)的肠道激素。在一项小型试验中,他证明了注射该激素会导致人的体重减轻。这一发现具有广阔的前景,他和一位合作者申请了专利。礼来公司只需利用该技术开发一种产品,就能拥有这种体重管理产品至少10年的独家销售权。

关键词：独家销售制药公司减肥药小型试验礼来公司迪马技术开发体重管理

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gFACs:Gene Filtering,Analysis,and Conversion to Unify Genome Annotations Across Alignment and Gene Prediction Frameworks

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Genomics, Proteomics & Bioinformatics 2019年第3期17卷 305-310页

作者： madison Caballero Jill Wegrzyn Department of Ecology and Evolutionary Biology University of Connecticut

Published genomes frequently contain erroneous gene models that represent issues associated with identification of open reading frames,start sites,splice sites,and related structural *** source of these inconsistencies is often traced back to integration across text file formats designed to describe long read alignments and predicted gene *** addition,the majority of gene prediction frameworks do not provide robust downstream filtering to remove problematic gene annotations,nor do they represent these annotations in a format consistent with current file *** frameworks also lack consideration for functional attributes,such as the presence or absence of protein domains that can be used for gene model *** provide oversight to the increasing number of published genome annotations,we present a software package,the Gene Filtering,Analysis,and Conversion(gFACs),to filter,analyze,and convert predicted gene models and *** software operates across a wide range of alignment,analysis,and gene prediction files with a flexible framework for defining gene models with reliable structural and functional *** supports common downstream applications,including genome browsers,and generates extensive details on the filtering process,including distributions that can be visualized to further assess the proposed gene *** is freely available and implemented in Perl with support from BioPerl libraries at https://***/PlantGenomicsLab/gFACs.

关键词： Genome annotation Bioinformatics Protein annotation Gene prediction Alignment

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Live-Cell Imaging of Dual-Labeled Golgi Stacks in Tobacco BY-2 Cells Reveals Similar Behaviors for Different Cisternae during Movement and Brefeldin A Treatment

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Molecular Plant 2011年第5期4卷 896-908页

作者： Stephanie L. madison Andreas Nebenfuhr Department of Biochemistry and Cellular and Molecular Biology University of Tennessee Knoxville TN 37996-0840 USA

In plant cells, the Golgi apparatus consists of numerous stacks that, in turn, are composed of several flattened cisternae with a clear cis-to-trans polarity. During normal functioning within living cells, this unusual organelle displays a wide range of dynamic behaviors such as whole stack motility, constant membrane flux through the cisternae, and Golgi enzyme recycling through the ER. In order to further investigate various aspects of Golgi stack dynamics and integrity, we co-expressed pairs of established Golgi markers in tobacco BY-2 cells to distinguish sub-compartments of the Golgi during monensin treatments, movement, and brefeldin A （BFA）-induced disassembly. A combination of cis and trans markers revealed that Golgi stacks remain intact as they move through the cytoplasm. The Golgi stack orientation during these movements showed a slight preference for the cis side moving ahead, but trans cisternae were also found at the leading edge. During BFA treatments, the different sub-compartments of about half of the observed stacks fused with the ER sequentially; however, no consistent order could be detected. In contrast, the ionophore monensin resulted in swelling of trans cisternae while medial and particularly cis cisternae were mostly unaffected. Our results thus demonstrate a re- markable equivalence of the different cisternae with respect to movement and BFA-induced fusion with the ER. In addi- tion, we propose that a combination of dual-label fluorescence microscopy and drug treatments can provide a simple alternative approach to the determination of protein localization to specific Golgi sub-compartments.

关键词： Golgi apparatus Golgi stack integrity brefeldin A monensin tobacco BY-2 cells live-cell imaging.

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Public Risk Perception Attitude and Information-Seeking Efficacy on Floods:A Formative Study for Disaster Preparation Campaigns and Policies

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International Journal of Disaster Risk Science 2020年第5期11卷 592-601页

作者： Do Kyun David Kim T.Phillip madison Department of Communication University of Louisiana at LafayetteLafayette.LA 70503USA

Floods are among the most frequent and devastating natural hazards and disasters in many southern states in the United *** study examined the relationship and reciprocal predictability between two theoretical constructs-risk perception attitude(RPA)and informationseeking efficacy(ISE)——in regard to pluvial *** addition,this study extended these theoretical constructs to investigate differences in RPA and ISE among potential audience segments,providing practitioners with applicable insights for designing effective flood prevention and risk management *** of data from 716 residents in south Louisiana revealed a statistically strong relationship between RPA and *** research also identified specific audience segments that would benefit from an increase in RPA and ISE concerning *** meaningful findings infonn a discussion of the theoretical and practical implications of the relationship between RPA and ISE and guide future disaster preparation campaigns and policies.

关键词： Communication campaign Disaster preparation Flood victims Information-seeking efficacy Pluvial flood Risk perception attitude

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Consumers’experiences and preferences for plant-based meat food:Evidence from a choice experiment in four cities of China

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Journal of Integrative Agriculture 2023年第1期22卷 306-319页

作者： WANG Ge madison T PLASTER Bai Yun-li LIU Cheng-fang China Center for Agricultural Policy School of Advanced Agricultural SciencesPeking UniversityBeijing 100871P.R.China Yenching Academy Peking UniversityBeijing 100871P.R.China Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101R.P.China United Nations Environment Programme–International Ecosystem Management Partnership Beijing 100101R.P.China

This paper examined consumers’experiences in and preferences for plant-based meat(PBM)food and their respective correlates,based on data from an online survey of 579 consumers in four major cities in China in early *** first described consumers’experiences in consuming and purchasing PBM food and their correlates,and then analyzed consumer preferences using hypothetical choice *** experiment offered consumers various options to purchase burgers made from PBM or animal-based meat(ABM),combined with different countries of origin(COO),taste labels,and *** data showed that respondents hold overall positive attitudes toward PBM food;85 and 82%of respondents reported experience in eating and purchasing PBM food,*** than half of them ate PBM food because they wanted to try new food(58%),or were interested in healthy food(56%).Income,religion,and dietary restrictions were significantly correlated with consumers’experiences in PBM food *** from the Random Parameter Logit Model based on the hypothetical choice experiment data showed that 79%of respondents chose PBM burgers and were willing to pay an average of 88 CNY for a PBM *** also found that 99.8 and 83%of respondents are willing to buy burgers made in China and those with a taste label,with a willingness to pay(WTP)of 208 and 120 CNY,*** heterogeneity test revealed that females and those with at least a bachelor’s degree,higher income,religious beliefs,and dietary restrictions are more likely to buy PBM burgers than their counterparts.

关键词： plant-based meat(PBM) choice experiment consumers’preference willingness to pay

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An OGCM Simulation of Seasonal and Interannual Variabilities in the Surface-Layer Pacific of the Equatorial Band

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Advances in Atmospheric Sciences 2002年第2期19卷 219-235页

作者： Boyin Huang and Zhengyu Liu Department of Atmospheric and Oceanic Sciences, University of Wisconsin-madison 1225 W. Dayton St., madison, WI-53706, U.S.A. University of Wisconsin-madison

The heat budget is analyzed in the surface-layer (0-50 m) Pacific of the equatorial band (10°S-10°N), using the simulation of an ocean general circulation model from 1945 to 1993. The analysis indicates that downward net surface heat flux from the atmosphere and ocean advective heat fluxes play distinct roles in seasonal and interannual variabilities of surface-layer ocean temperature. The surface heat flux dominantly determines the ocean temperature in the seasonal time-scale. But, it has a negative feedback to the ocean temperature in the interannual time-scale. The interannual variability of ocean temperature is largely associated with the cold advection from off-equatorial divergent flow in the central Pacific and from upwelling in the cold tongue. Both the surface heat flux and ocean advective heat fluxes are important to the ocean temperature during an El Nino event. The ocean. advective heat fluxes are further associated with local westward trade wind in the central Pacific. These results are largely consistent with some regional observational analyses.

关键词： Seasonal variability Interannual variability Heat flux

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Periorbital rejuvenation in the clinic:A state-of-the-art review

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2023年第3期9卷 242-248页

作者： Sarah M.Russel J.madison Clark Department of Otolaryngology Head and Neck Surgery University of North Carolina at Chapel HillChapel HillNorth CarolinaUSA Division of Facial Plastic and Reconstructive Surgery Department of Otolaryngology Head and Neck SurgeryUniversity of North Carolina at Chapel HillChapel HillNorth CarolinaUSA

Objective:To provide an overview of the various treatment options available in the clinic to achieve periorbital *** Sources:Literature review and expert ***:Periorbital rejuvenation in the clinic can be accomplished through a variety of treatment modalities,including topical therapies,skin resurfacing,and fillers and ***,some surgical approaches,such as upper blepharoplasties,ptosis,and brow lifts,can be performed in the clinic under local anesthesia with only mild oral ***,the successful execution of such procedures depends on proper patient selection and maximizing patient comfort.

关键词： blepharoplasty chemexfoliation laser therapy rejuvenation skin aging

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