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Local changes in chromatin Accessibility and Transcriptional Networks Underlying the Nitrate Response in Arabidopsis Roots

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Molecular Plant 2019年第12期12卷 1545-1560页

作者： jose M.Alvarez Tomas c.Moyano Tao Zhang Diana E.Gras Francisco J.Herrera Viviana Araus jose AQ'Brien Laura carrillo Joaquin Medina Jesus Vicente-carbajosa Jiming Jiang Rodrigo A.Gutierrez Pontificia Universidad catolica de chile SantiagoChile Yangzhou University YangzhouChina Instituto de Agrobiotecnologia del Litoral CONICETSanta FeArgentina University of california BerkeleyCAUSA Trancura Biosciences Inc.San FranciscoCA 94158USA centro de Biotecnología y Genómica de Plantas(cBGP UPM-INIA)Universidad Politecnica de Madrid(UPM)Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA)Campus de Montegancedo28223-Pozuelo de AlarcóMadridSpain Department of Horticulture University of Wisconsin-MadisonMadisonWI 53706USA Department of Plant Biology and Horticulture Michigan State UniversityMI 48824USA

Transcriptional regulation,determined by the chromatin structure and regulatory elements interacting at pro-moter regions,is a key step in plant responses to environmental ***(NO3-)is a nutrient signal that regulates the expression of hundreds of genes in Arabidopsis ***,we integrate mRNA sequencing,genome-wide RNA polymeraseⅡ(RNPⅡ),chromatin immunoprecipitation sequencing,and DNase Sequencing datasets to establish the relationship between RNPⅡoccupancy and chromatin accessibility in response to NO3-treatments in Arabidopsis *** footprinting allowed us to identify in vivo regula-tory elements controlling gene expression in response to ***3--modulated transcription factor(TF)footprints are important for a rapid increase in RNPⅡoccupancy and transcript accumulation over *** mapped key TF regulatory interactions and functionally validated the role of NAP,an NAc-domain containing TF,as a new regulatory factor in *** together,our study provides a comprehensive view of transcriptional networks in response to a nutrient signal in Arabidopsis roots.

关键词： nitrate transcriptional regulation footprinting RNA polymeraseⅡ regulatory networks

Petiveria alliacea Suppresses Airway Inflammation and Allergen-Specific Th2 Responses in Ovalbumin-Sensitized Murine Model of Asthma

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chinese Journal of Integrative Medicine 2018年第12期24卷 912-919页

作者： Rosa martha Perez Gutierrez jose maria Mota Flores Natural Products Research Laboratory Higher School of Chemical Engineering and Extractive IndustriesInstituto Politecnico Nacional (IPN) Professional Unit Adolfo Lopez Mateos Av. Instituto Politécnico NacionalS/N

Objective： To examine the effect of metanol extract of Petiveria alliacea （PM） on airway inflammation in a murine model of chronic asthma. Methods： Two-month-old male BALB/c mice （n=6-8/group） were sensitized on days 0 and 14 by intraperitoneal injection of 20μg ovalbumin （OVA）. On day 25, the mice received an airway challenge with OVA （3%, w/v, in phosphate buffered saline）. PM was administered orally by oral gavage to mice at doses of 100, 200 and 400 mg/kg body weight once daily from days 18 to 23. control mice were orally administered phosphate buffered saline （PBS） to induce a model of asthma. At the end of the test, respiratory reactivity was assayed, the total cell number, interleukin-4 （IL-4）, IL-5, IL-13, tumor necrosis factor-alpha （TNF-α） and reactive oxygen species （ROS） in the bronchoalveolar lavage fluid （BALF） were determined and the levels of serum IgE, intercellular cell adhesion molecule 1 （IcAM-1） and eotoxin were measured. In addition, lung tissue was used to qualify the IL-4, IL-5, IL-13, TNF-α and transforming growth factor beta 1 （TGF-β1）. Histologic examination was performed to observe inflammatory cellular infiltration. Results： The administration of PM in comparison with the OVA-only treated group significantly attenuated the infiltration of eosinophUs and other inflammatory cells （P〈0.01）. Airway resistance （RI） in the OVA-only induced group was significantly higher than that of the PBS control group （P〈0.01） when methacholine was added. TNF-α, IgE, TGF-β1 and cytokine levels IL-4, IL-5, IL-β in the BALF decreased compared to control mice （P〈0.01 or P〈0.05）. PM treatment also inhibited the production of chemokines, eotaxin and IcAM-1 in BALF （P〈0.01）, which improved lung function. Histopathological examination revealed that the sensitized treated PM groups had significant lower in inflammatory scores similar to dexamethasone treatments and the untreated group. conclusion： Administration of PM could inhibit airway inflammation, regulate cyt

关键词： Petiveria alliacea allergy antiinflammation asthma

Expression of juvenile hormone acid O-methyltransferase and juvenile hormone synthesis in Blattella germanica

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Insect Science 2018年第5期25卷 787-796页

作者： claudia V. Dominguez jose L. maestro Institute of Evolutionary Biology CSIC-Universitat Pompeu Fabra Barcelona Spain

Juvenile hormone （JH）, a sesquiterpenoid synthetized by the insect corpora allata （cA）, plays critical roles in metamorphosis and reproduction. Penultimate or last step of JH synthesis is catalyzed by juvenile hormone acid O-methyltransferase （JHAMT）. Here we report the cloning and expression analysis of the JHAMT orthologue in the cockroach, Blattella germanica （L.）（BgJHAMT）. BgJHAMT is mainly expressed in cA, with only expression traces in ovary. Three different isoforms, differing in the 3＇-UTR sequence, were identified. Isoform A shows between 35 and 65 times higher expression than B and c in cA from penultimate nymphal instar and adult females. RNAi-triggered knock down of BgJHAMT produces a dramatic reduction of JH synthesis, concomitant with a decrease of fat body viteUogenin expression and basal follicle length. BgJHAMT mRNA levels in cA of females along the gonadotrophic cycle parallel, with a slight advancement, JH synthesis profile. BgJHAMT mRNA levels were reduced in starved females and in females in which we reduced nutritional signaling by knocking down insulin receptor and target of rapamycin （TOR）. Results show that conditions that modify JH synthesis in adult B. germanica females show parallel changes of BgJHAMT mRNA levels and that the JH-specifie branch of the JH synthesis pathway is regulated in the same way as the mevalonate branch. Furthermore, we demonstrate that nutrition and its signaling through the insulin receptor and TOR pathways are essential for activating BgJHAMT expression, which suggests that this enzyme can be a checkpoint for the regulation of JH production in relation to nutritional status.

关键词： Blattella germanica corpora allata JHAMT juvenile hormone nutritionalsignaling

Excretion of biliary compounds during intrauterine life

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World Journal of Gastroenterology 2009年第7期15卷 817-828页

作者： Rocio IR macias jose JG marin maria A Serrano Laboratory of Experimental Hepatology and Drug Targeting CIBERehdUniversity of Salamanca

In adults,the hepatobiliary system,together with the kidney,constitute the main routes for the elimination of several endogenous and xenobiotic compounds into bile and urine,***,during intrauterine life the biliary route of excretion for cholephilic compounds,such as bile acids and biliary pigments,is very *** very early in pregnancy the fetal liver produces bile acids,bilirubin and biliverdin,these compounds cannot be efficiently eliminated by the fetal hepatobiliary system,owing to the immaturity of the excretory machinery in the fetal ***,the potentially harmful accumulation of cholephilic compounds in the fetus is prevented by their elimination across the *** to the presence of detoxifying enzymes and specifi c transport systems at different locations of the placental barrier,such as the endothe-lial cells of chorionic vessels and trophoblast cells,this organ plays an important role in the hepatobiliary-like function during intrauterine *** relevance of this excretory function in normal fetal physiology is evident in situations where high concentrations of biliary compounds are accumulated in the *** may result in oxidative stress and apoptosis,mainly in the placenta and fetal liver,which might affect normal fetal development and challenge the fate of the *** present article reviews current knowledge of the mechanisms underlying the hepatobiliary function of the fetal-placental unit and the repercussions of several pathological conditions on this tandem.

关键词： Bile acids Bilirubin Foetus Liver Placenta

Antioxidant effects of D-004, a lipid extract from the Roystonea regia fruit, on the plasma of healthy men

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Asian Journal of Andrology 2009年第3期11卷 385-392页

作者： Emesto Lopez Vivian Molina jose Illnait Ambar Oyarzfibal Lilia c. Femandez Rosa mas Rafael Gamez Julio c. Fernandez Sonia Jimenez Meilis Mesa Ivon Hollands Sarahi Mendoza Medical Surgical Research centre Havana 6990 Cuba centre of Natural Products from the National centre for Scientific Research Havana 6880 Cuba

The aim of thisstudy was to conduct a randomized, double-blind and placebo-controlled study to investigate the effects of D-004, a lipid extract of the Roystonea regia fruit that prevents testosterone- and phenylepinephrine- induced prostate hyperplasia in rodents, on plasma oxidative markers in healthy men. We enrolled male volunteers （20-55 years） in good health and without lower urinary tract symptoms. Thirty-four eligible participants were randomized to placebo or D-004 （320 mg） capsules administered daily for 6 weeks. An interim check-up and a final visit were conducted after 3 and 6 weeks of therapy, respectively. Physical examinations were performed at each visit, and laboratory tests were performed at baseline and at treatment completion. Oxidative variables included plasma malondialdehyde （MDA）, total hydroxyperoxides （TOH）, sulphydryl （SH） groups and total antioxidant status （TAS）. We assessed treatment compliance and addressed adverse experiences （AEs） at weeks 3 and 6. At week 6, with D-004, the mean reductions of plasma MDA （26.7%）, TOH （18.8%） and SH groups （31.6%）, and the mean increase of TAS （35.3%） were significantly different from those of placebo （P 〈 0.001 for plasma TAS, P 〈 0.0001 for all other comparisons）. D-004 did not differ from the placebo in safety indicators. There were two withdrawals （both in the D-004 group）, with one due to dyspepsia （the only AE during the trial）. In conclusion, D-004 displayed antioxidant effects on plasma oxidative markers in healthy men, which was consistent with findings from laboratory experimental studies.

关键词： antioxidant benign prostate hyperplasia D-004 lipid peroxidation Roystonea regia

Soil-transmitted helminth egg contamination from soil of indigenous communities in selected barangays in Tigaon, camarines Sur, Philippines

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Asian Pacific Journal of Tropical Medicine 2020年第9期13卷 409-414页

作者： James Owen c.Delaluna mary Jane c.Flores Vicente Y.Belizario Jr. jose Isagani B.Janairo Derick Erl P.Sumalapao Biology Department College of ScienceDe La Salle UniversityManila Philippines Biological control Research Unit Center for Natural Sciences and Environmental ResearchDe La Salle UniversityManila Philippines Department of Parasitology College of Public HealthUniversity of the PhilippinesManila Philippines Department of Epidemiology and Biostatistics College of Public HealthUniversity of the PhilippinesManila Philippines

Objective:To provide baseline data on the prevalence and intensity of soil-transmitted helminthiasis egg contamination in the soil among indigenous ***:A total of 317 soil samples from three barangays of indigenous communities communities in Tigaon,camarines Sur,Philippines were examined for soil-transmitted helminthiasis egg contamination using optimized sugar flotation ***:Of the soil samples examined,141(44.48%)were contaminated by Ascaris spp.,Toxocara spp.,and Trichuris *** cumulative prevalence varying across the study sites(Pcaris *** predominant in all study sites,followed by Toxocara *** Trichuris *** a prevalence of 41.96%,7.57%,and 5.36%,***,Toxocara *** the highest intensity of contamination,followed by Ascaris *** Trichuris *** term of geometric mean soil-transmitted helminthiasis eggs recovered per one gram soil sample(34.25,21.45,and 11.85 respectively).Each study site harbors significant amount of soiltransmitted helminthiasis eggs and zoonotic Toxocara eggs,which present high risk of soil-transmitted helminthiasis infection,particularly among children observed to play and cohabitate with animals known to be hosts of these ***:The alarming rate of soil-transmitted helminthiasis and Toxocara egg contamination reported in this study suggests that additional measures should be undertaken to control soil-transmitted helminthiasis and zoonotic intestinal infections in the country.

关键词： Indigenous peoples Soil-transmitted helminths Ascaris Toxocara Trichuris Public health

Visual grading criteria for Japanese larch ( Larix kaempferi ) structural timber from Spain

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Journal of Forestry Research 2020年第6期31卷 2605-2614页

作者： mari-jose Barriola José-Ramón Aira Edgar Lafuente Tknika LHri Aplikatutako Ikerketa eta Berrikuntzako EAEko zentroaZamalbide Auzoa z/g20100 ErrenteriaGipuzkoaSpain Escuela de Ingeniería de la Industria Forestal Agronómica y de la BioenergíaUniversidad de ValladolidUVa-Campus Duques de Sorias/n42005 SoriaSpain cesefor Centro de Servicios y Promoción Forestal y de su Industria de Castilla y LeónCalle C42005 SoriaSpain

Larch wood is structurally classifi ed in many countries as one of conifers with the highest load-bearing capacity(strength class of c30).The Spanish visual classifi cation regulation only assigns a strength class to 4 pine woods:Laricio pine(Pinus nigra ***),Silvestre pine(Pinus sylvestris L.),Radiata pine(Pinus radiata ***),and Pinaster pine(Pinus pinaster Ait.).This work adds to the number of structurally characterised species by creating a visual classifi cation table for Japanese larch wood(Larix kaempferi(Lamb.)carr.)which diff erentiates between 2 visual classes,MEG-1 and *** strength values were calculated for each class(fk,MEG-1=31.80 MPa,f k,MEG-2=24.55 MPa),mean module of elasticity(E 0,mean,MEG-1=13,082 MPA,E 0,mean,MEG-2=12,320 MPA)and density(ρk,MEG-1=456.6 kg m−3,ρk,MEG-2=469.1 kg m−3),before fi nally assigning a strength class of c30 to visual class MEG-1,and a strength class of c24 to visual class MEG-2.

关键词： Japanese larch wood Visual grading Strength class Mechanical properties Density

Simultaneous Biparametric Determination of Total calcium and Potassium in Biological Fluids by Flow Injection Analysis──Use of Powell＇s Method in the System Optimization

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chemical Research in chinese Universities 1994年第1期10卷 5-15页

作者： Araujo, Alberto N. Jun, Shiao Lima, jose L.F.c. Alonso, Julian Poch, manel del Valle, manel

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ＳｉｍｕｌｔａｎｅｏｕｓＢｉｐａｒａｍｅｔｒｉｃＤｅｔｅｒｍｉｎａｔｉｏｎｏｆＴｏｔａｌＣａｌｃｉｕｍａｎｄＰｏｔａｓｓｉｕｍｉｎＢｉｏｌｏｇｉｃａｌＦｌｕｉｄｓｂｙＦｌｏｗＩｎｊｅｃｔｉｏｎＡｎａｌｙｓｉｓ──ＵｓｅｏｆＰｏｗｅｌｌ＇ｓＭｅｔｈｏｄ...

关键词： Flow-injection analysis Two-component determination Potassiumand calcium Blood serum Powell's optimization

crisis challenges of small firms in macao during the cOVID-19 pandemic

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Frontiers of Business Research in china 2020年第4期14卷 403-425页

作者： jose cAlves Tan cheng Lok Yubo Luo and Wei Hao city University of macao 4th FloorLuso-Chinese BuildingAvenida Padre Tomas PereiraMacaoChina

This research develops a framework that combines crisis stages,stakeholder engagement and crisis *** framework is applied to small firms in macao during the 2019 novel coronavirus disease(cOVID-19)pandemic *** conduct a qualitative study based on semi-structured interviews with the leaders of six small firms in *** findings suggest that the cOVID-19 pandemic has turned into a"normal"context,which blurs the traditional crisis termination *** also find that participating firms engage more with internal stakeholders than external *** strategies adopted by small firms include flexible human resource(HR)practices,cost reduction,enhancing customer relations,and using government support *** strategies are effective in the short term;firms need to pay attention to diversity and learning for the long term.

关键词： crisis development crisis strategies Small firms 2019 novel coronavirus disease(cOVID-19) macao