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Science China Life Sciences 2024年

作者： Hongyu Li qingwen ma Yan Xue Linlin Cai Liwen Bao Lei Hong Yitao Zeng Shuzhen Huang Richard H.Finnell Fanyi Zeng Shanghai Institute of Medical Genetics Shanghai Children's Hospital Shanghai Jiao Tong University School of Medicine Department of Histo-Embryology Genetics and Developmental Biology Shanghai Jiao Tong University School of Medicine NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology Shanghai Key Laboratory of Embryo and Reproduction Engineering Center for Precision Environmental Health Baylor College of Medicine School of Pharmacy Macau University of Science and Technology

Autosomal recessive spinocerebellar ataxias（SCARs） are one of the most common neurodegenerative diseases characterized by progressive ataxia. Although SCARs are known to be caused by mutations in multiple genes, there are still many cases that go undiagnosed or are misdiagnosed. In this study, we presented a SCAR patient, and identified a probable novel pathogenic mutation（c.1A>G, p.M1V） in the AFG3L2 start codon. The proband's genotype included heterozygous mutations of the compound AFG3L2(p.[M1V]; [R632X]（c.[1A>G];[1894.C>T]）), which were inherited from the father（c.1A>G, p.M1V） and mother（c.1894C>T, p.R632X）. Functional studies performed on hiPSCs（human induced pluripotent stem cells） generated from the patients and HEK293T cells showed that the mutations impair mitochondrial function and the unbalanced expression of AFG3L2 mRNA and protein levels. Furthermore, this novel mutation resulted in the degradation of the protein and the reduction of the stability of the AFG3L2 protein, and MCU（mitochondrial calcium uniporter） complex mediated Ca2+overload.

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Dynamical Mechanisms of Effects of Landslides on Long Distance Oil and Gas Pipelines

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Wuhan University Journal of Natural Sciences 2006年第4期11卷 820-824页

作者： ma qingwen WANG Chenghua KONG Jiming Institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu 610041 Sichuan China Graduate University of Chinese Academy of Sciences Beijing 100049 China

According to the investigations on the oil and gas pipelines such as the Lan-Cheng-Chong pipeline and the Southwest pipeline, there are two ways of laying pipeline： pipelines paralleling （approximately） to the main slide direction and pipelines perpendicular （approximately） to the main slide direction. If earth-retaining walls have been built for pipelines paralleling to the main slide direction, they will prevent the lands from sliding; On the contrary, without earth-retaining walls, the sharp broken rocks in the backfilling soil will scratch the safeguard of the pipeline when the landslides take place. Pipelines perpendicular to the main slide direction can be classified into four types according to the relative positions between pipelines and landslides： Pipelines over the slide planes, pipelines inside the fracture strips of slide planes, pipelines below the slide planes and pipelines behind the backsides of landslides. The different dynamical mechanisms of the process in which landslide acts against pipelines are analyzed based on whether the pipelines are equipped with fixed frusta, because the sliding resistance depends on whether and how many fixed frusta are equipped and the distance between frusta.

关键词： landslide long distance oil and gas pipeline dynamical mechanism

Criterions and Measures of Route Selection of Shallowly Embedded Long-Distance Oil and Gas Pipeline in Mountain Areas

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Wuhan University Journal of Natural Sciences 2006年第4期11卷 787-792页

作者： WANG Chenghua ma qingwen KONG Jiming CHEN Zefu LI Xiuzhen Institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu 610041 Sichuan China Graduate university of Chinese Academy of Sciences Beijing 100049 China

According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows： the flat landform is the first choice in route alignment. The foot of mountain is the first choice when the route passes by the valley. The route should pass by but the shady and deposited slope and not in sunny and erosive slope as possible as it can. The pipeline should be vertical to contour climbing and descending the mountain except steep slope. Tunnel can be used in crossing foothill. Perpendicularly traversing the river is better than beveling; the worst choice is to put the pipeline along the river. Bypass is the best choice in karsts area. The order of route selection should be pre-choosing, investigation, optimization and adjustment.

关键词： shallowly embedded long-distance oil and gas pipeline criterions and measures of route selection avoiding geological hazards

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肉苁蓉寄主梭梭和白梭梭主要生物学性状评价

植物遗传资源学报 2024年第8期25卷 1347-1355页

作者：沈亮庞青云张明慧徐荣陈君刘同宁马清温刘永萍国家自然博物馆中国医学科学院北京协和医学院药用植物研究所道地药材品质保障与资源持续利用全国重点实验室北京城市学院生物医药学部宁夏永宁县本草苁蓉种植基地新疆林业科学院造林治沙研究所

采用田间农艺观察、单因素方差分析、相关性分析和分子鉴定等方法对我国梭梭属2个物种梭梭和白梭梭的主要生物学性状以及肉苁蓉寄生情况进行比较分析。结果表明梭梭和白梭梭在形态特征、农艺性状及花粉结构方面均存在显著差异，其中成... 详细信息

采用田间农艺观察、单因素方差分析、相关性分析和分子鉴定等方法对我国梭梭属2个物种梭梭和白梭梭的主要生物学性状以及肉苁蓉寄生情况进行比较分析。结果表明梭梭和白梭梭在形态特征、农艺性状及花粉结构方面均存在显著差异，其中成熟的梭梭种子果翅长度和千粒重显著低于白梭梭，但发芽率和发芽势显著高于白梭梭；梭梭花粉网眼较深，网脊颗粒结构的数量显著少于白梭梭，且花粉体积小于白梭梭。相关分析表明梭梭和白梭梭农艺指标与气温和相对湿度等气候因子显著相关，其中梭梭对水分及光照等环境因子变化适宜能力较强，分布较广；而白梭梭受水分等因子限制，仅在新疆准噶尔盆地长势较好。核基因内转录间隔区（ITS,internal transcribed spacer）分子鉴定技术可以有效鉴别梭梭和白梭梭，并且证明二者均可被肉苁蓉寄生，但二者寄生的肉苁蓉持续成活率存在差异。根据梭梭和白梭梭生物学性状特征和肉苁蓉寄生情况，适宜推广梭梭作为寄主植物生产肉苁蓉药材。本研究为我国梭梭属植物的分类鉴定、引种栽培以及肉苁蓉优良寄主种质筛选提供科学依据。

关键词：梭梭白梭梭肉苁蓉花粉 ITS

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木质素基复合膜的研究进展

中国造纸学报 2024年第2期39卷 83-91页

作者：李鑫穆瀚东毛壮壮刘纾彤马庆玟许桂娟刘姗姗王强齐鲁工业大学(山东省科学院)生物基材料与绿色造纸国家重点实验室山东济南250353 潍坊汇胜绝缘技术有限公司山东潍坊261201

木质素作为自然界中储量最为丰富的芳香族聚合物,可以赋予复合膜材料良好的机械强度,以及抗氧化、抗紫外等功能。本文介绍了木质素结构特点和改性方法,重点论述了木质素及其衍生物在聚合物复合膜材料中应用的研究进展,包括木质素作为填... 详细信息

木质素作为自然界中储量最为丰富的芳香族聚合物,可以赋予复合膜材料良好的机械强度,以及抗氧化、抗紫外等功能。本文介绍了木质素结构特点和改性方法,重点论述了木质素及其衍生物在聚合物复合膜材料中应用的研究进展,包括木质素作为填充物增强复合膜材料的机械性能,以及木质素作为改性剂提高复合膜材料的阻隔性和热稳定性等;展望了木质素及其衍生物应用于高性能复合膜材料的研究方向及应用潜力。

关键词：木质素改性聚合物复合膜材料

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Activation of proHGF by St14 induces mouse embryonic stem cell differentiation

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Protein & Cell 2016年第8期7卷 601-605页

作者： Yan, Xiaoshuang Xue, Yan Zhou, Yiye Cheng, Yan Yin, Shang ma, qingwen Zeng, Fanyi Shanghai Jiao Tong Univ Sch Med Inst Med Sci Shanghai 200025 Peoples R China Shanghai Jiao Tong Univ Shanghai Childrens Hosp Shanghai Inst Med Genet Shanghai 200040 Peoples R China Minist Hlth Key Lab Med Embryo Mol Biol Shanghai 200040 Peoples R China Shanghai Lab Embryo & Reprod Engn Shanghai 200040 Peoples R China

Embryonic stem cells （ESCs） are capable of unlimited selfrenewal and differentiation, thus generating remarkable interest in their potential therapeutic use in cell-based tissue engineering and regeneration. Stem cell based therapy has been considered as the most promising therapeutic strategy for a range of debilitating diseases （Reynolds and Lamba, 2014）.

关键词： Hepatocyte Growth Factor Pluripotent Gene Protease System Hepatocyte Growth Factor Activator Transmembrane Serine Protease

A Profile of Native Integration Sites Used by φC31 Integrase in the Bovine Genome

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Journal of Genetics and Genomics 2012年第5期39卷 217-224页

作者： Lijuan Qu qingwen ma Zaiwei Zhou Haiyan ma Ying Huang Shuzhen Huang Fanyi Zeng Yitao Zeng Shanghai Institute of Medical Genetics Children＇s Hospital of Shanghai Shanghai Jiao Tong University School of Medicine 24/1400 West Beijing Road Shanghai 200040 China Key Lab of Medical Embryo Molecular Biology Ministry of Health Shanghai 200040 China Shanghai Key Lab of Embryo and Reproduction Engineering Shanghai Municipal Committee of Science and Technology Shanghai 200040 China Institute of Medical Science Shanghai Jiao Tong University School of Medicine 280 South Chongqing Road Shanghai 200025 China

The Streptomyces phage φC31 integrase can efficiently target attB-bearing transgenes to endogenous pseudo attP sites within mammalian genomes. To better understand the activity of φC31 integrase in the bovine genome, DNA sequences of 44 integration events were analyzed, and 32 pseudo attP sites were identified. The majority of these sites share a sequence motif that contains inverted repeats and has similarities to wild-type attP site. Genomic DNA flanking these sites typically contained repetitive sequence elements, such as short and long interspersed repetitive elements. These sequence features indicate that DNA sequence recognition plays an important role in guiding φC31-mediated site-specific integration. In addition, BF27 integration hotspot sites were identified in the bovine genome, which accounted for 13.6% of all isolated integration events and mapped to an intron of the deleted in liver cancer 1 （DLC1） gene. Also we found that the pseudo attP sites in the bovine genome had other features in common with those in the human genome. This study represents the first time that the sequence features of pseudo attP sites specific integrase system has great potential for applied modifications in the bovine genome were analyzed. We conclude that this site- of the bovine genome.

关键词： φC31 integrase Pseudo attP sites Sequence motif Repetitive elements Bovine genome

Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning

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Journal of Genetics and Genomics 2011年第1期38卷 21-28页

作者： Hao Yan Zhonghai Yan qingwen ma Fei Jiao Shuzhen Huang Fanyi Zeng Yitao Zeng Shanghai Institute of Medical Genetics Shanghai Jiao Tong University 24/1400 West Beijing Road Shanghai 200040 China The Key Lab of Embryo Molecular Biology Ministry of Health China and Shanghai Lab of Embryo and Reproduction Engineering 24/1400 West Beijing Road Shanghai 200040 China Institute of Medical Science Shanghai Jiao Tong University School of Medicine 280 South Chongqing Road Shanghai 200025 China

Reconstructed embryos derived from intersubspecies somatic cell nuclear transfer （SCNT） have poorer developmental potential than those from intrasubspecies SCNT. Based on our previous study that Holstein dairy bovine （HD） mitochondrial DNA （mtDNA） haplotype compatibility between donor karyoplast and recipient cytoplast is crucial for SCNT embryo development, we performed intersubspecies SCNT using HD as donor karyoplast and Luxi yellow heifer （LY） as recipient cytoplast according to mtDNA haplotypes determined by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） analysis. The results demonstrated that intersubspecies mtDNA homotype SCNT embryos had higher pre- and post-implantation developmental competence than intrasubspecies mtDNA heterotype embryos as well as improved blastocyst reprogramming status, including normal H3K9 dimethylation pattern and promoter hypomethylation of pluripotent genes such as Oct4 and Sox2, suggesting that intersubspecies SCNT using LY oocytes maintains HD cloning efficiency and may reprogram HD nuclei to develop into a normal cloned animal ultimately. Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HD cloning.

关键词： Somatic cell nuclear transfer (SCNT) Mitochondria mtDNA haplotype Epigenetic modification Bovine Intersubspecis Intrasubspecis Developmental competence

Rapid surface texturing to achieve robust superhydrophobicity,controllable droplet impact,and anti-frosting performances

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Friction 2024年第2期12卷 291-304页

作者： qingwen DAI Lei CHEN Jiabao PAN Liping SHI Dameng LIU Wei HUANG Xiaolei WANG College of Mechanical and Electrical Engineering Nanjing University of Aeronautics&AstronauticsNanjing 210016China State Key Laboratory of Tribology in Advanced Equipment Department of Mechanical EngineeringTsinghua UniversityBeijing 100084China School of Mechanical Engineering Anhui Polytechnic UniversityWuhu 241000China School of Mechanical Engineering Anhui University of TechnologyMa’anshan 243032China

Robust superhydrophobic surfaces with excellent capacities of repelling water and anti-frosting are of importance for many mechanical *** this work,wear-resistant superhydrophobic surfaces were fabricated by curing a mixture of polyurethane acrylate(PUA)coating and 1H,1H,2H,2H-Perfluorodecyltrichlorosilane(HFTCS)on titanium alloy(TC4)surfaces decorated with micropillars pattern,thus,composite functional surfaces with PUA coating in the valleys around the micropillars pattern of TC4 were *** contact angle on fabricated surfaces could reach 167°.Influences of the geometric parameters of micropillars pattern on the apparent contact angle were investigated,and the corresponding wear-resistant property was *** impact and anti-frosting performances on the prepared surfaces were *** optimized design of surface texture with robust superhydrophobicity,controllable droplet impact,and anti-frosting performances was *** design principle is of promising prospects for fabricating superhydrophobic surfaces in traditional mechanical systems.

关键词： surface texture robust superhydrophobic wear-resistant droplet impact anti-frosting

基于多因素分析的烘丝机入口含水率预测模型的建立与应用

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烟草科技 2015年第5期48卷 67-73页

作者：钟文焱陈晓杜马庆文郭天文龙岩烟草工业有限责任公司

为了保障制丝过程中烘丝机入口含水率的稳定性,采用Pearson相关分析的方法,确定烘丝机入口含水率的主要影响因素,并用神经网络算法和多元回归分析方法建立含水率预测模型。通过模型求解,实现给定烘丝机入口含水率计算松散回潮机回潮加... 详细信息

为了保障制丝过程中烘丝机入口含水率的稳定性,采用Pearson相关分析的方法,确定烘丝机入口含水率的主要影响因素,并用神经网络算法和多元回归分析方法建立含水率预测模型。通过模型求解,实现给定烘丝机入口含水率计算松散回潮机回潮加水比例参考值的目的。采用模型预测值与实测值对比的方法进行检验。结果表明:烘丝机入口含水率设定值为19.2%时,采用本方法得到的烘丝机入口含水率均值为19.21%,优于改进前的19.09%,且误差标准偏差由0.43%降到0.26%,批次间烘丝机入口含水率的波动得到改善。

关键词：多因素分析烘丝机入口含水率相关分析温湿度加水比例