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Characterisation of pH decline and meat color development of beef carcasses during the early postmortem period in a Chinese beef cattle abattoir

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Journal of Integrative Agriculture 2018年第7期17卷 1691-1695页

作者： ZHANG Yi-min David L.Hopkins ZHAO Xiao-xiao Remy van de Ven mao yan-wei ZHU Li-xian HAN Guang-xing LUO Xin College of Food Science and Engineering Shandong Agricultural UniversityTai'an 271018P.R.China NSW Department of Primary Industries Centre for Red Meat and Sheep DevelopmentCowra 2794Australia NSW Department of Primary Industries Orange Agricultural InstituteForest RoadOrange 2800Australia Sishui Xinlv Food Co. Ltd.Jining 273200P.R.China

This study investigated the pH/temperature decline of beef carcasses in a typical Chinese abattoir and color development as pH declined during rigor onset. A natural cubic spline model was used to model the pH/temperature decline for those carcasses which passed through pH 6.0. Six of the 97 carcasses that exhibited a high （-〉6.10） ultimate pH （pHu）（darkcutting） in the M. Iongissimus lumborum （LL） were sampled, along with the same numbers of normal pHu and intermediate pHu carcasses （5.40-5.79; 5.80-6.10, respectively）, to examine color development within 24 h postmortem. It was shown that 66.7% of the modeled carcasses were outside the ideal pH/temperature window with a temperature@pH6.0 lower than ideal, suggesting the need for acceleration of the pH decline. The stable and low a^＊, b^＊ and chroma values of high pHu beef within the first 12 h indicated dark-cutting beef might be detected earlier than expected.

关键词： pH decline color ultimate pH dark-cutting beef

Implications of step-chilling on meat color investigated using proteome analysis of the sarcoplasmic protein fraction of beef longissimus lumborum muscle

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Journal of Integrative Agriculture 2018年第9期17卷 2118-2125页

作者： ZHANG Yi-min ZHANG Xiu-ze WANG Tian-tian David L.Hopkins mao yan-wei LIANG Rong-rong yanG Guang-fu LUO Xin ZHU Li-xian College of Food Science and Engineering Shandong Agricultural UniversityTai'an 2 71018P.R.China NSW Department of Primary Industries Centre for Red Meat and Sheep DevelopmentCowra 2794Australia Shandong Hongan(Group)Co. Ltd.Yangxin 251800P.R.China

In order to improve beef color and color stability, step-chilling （SC） was applied on excised bovine Iongissimus lumborum muscle, with chilling starting at 0-4℃ for 5 h, then holding the temperature at 12-18℃ for 6 h, followed by 0-4℃ again until 24 h post-mortem, pH and temperature were measured during rigor on SC loins as well as those subjected to routine chilling （RC, 0-4℃, till 24 h post-mortem）. Color L＊, a＊, b＊ values, metmyoglobin （MetMb） content, MetMb reducing ability （MRA） and NADH content were determined on samples aged for 1, 7, and 14 d. Sarcoplasmic proteome analysis was only conducted on d 1 samples. The results showed muscles subjected to SC maintained a temperature at around 15℃ for 5 to 10 h post-mortem, and exhibited a slow temperature decline, but rapid pH decline. Beef steaks treated with SC had higher L＊, a＊, b＊ and chroma values than those of RC samples at 1 and 7 d chilled storage （0-4℃）, while showing no significant difference for a＊, b＊ and chroma values at d 14. The SC samples also exhibited a lower relative content of surface MetMb, higher MRA and NADH content, compared with RC beef steaks during storage, indicating the SC-treated beef showed an improved color stability. Eleven differential protein spots/nine proteins were identified by two-dimensional gel electrophoresis and mass spectrometry, and those proteins were mainly involved in redox, chaperone binding, metabolic and peroxidase activity. Oxidoreductases play a role in decreasing the oxidation-induced myoglobin oxidation and benefiting the production of NADH, and finally improving the colour of beef. Of these, pyruvate dehydrogenase E1 component subunit beta showed a positive correlation with color L＊, a＊, b＊ values and accounted for more than 60% of the variation in color values; this protein can be considered as a potential beef color biomarker. The present study provided valuable information for studies on the molecular mechanism of color improvement from step-chilling, as wel

关键词： step-chilling beef color proteomics oxidoreductase

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西方古典伦理学视角下的“伪善”:似善非善

华中科技大学学报（社会科学版） 2016年第4期30卷 22-26,39页

作者：闫茂伟东南大学人文学院江苏南京211189

伪善研究的视角在西方语境与背景下呈现出多学科化,而在具有基础性的伦理学这一视角下,伪善研究直到现代才在道德哲学家那里受到重视;并且,"伪善"这一概念也被认为至少不是一个古典哲学的术语。然而,伪善的道德内涵以及与之有关的伪善... 详细信息

伪善研究的视角在西方语境与背景下呈现出多学科化,而在具有基础性的伦理学这一视角下,伪善研究直到现代才在道德哲学家那里受到重视;并且,"伪善"这一概念也被认为至少不是一个古典哲学的术语。然而,伪善的道德内涵以及与之有关的伪善现象与问题早在古希腊时期就得到了详尽的论述,尤其是苏格拉底和柏拉图的"‘善’的虚假印象"、亚里士多德的"显得是某种善的东西"为界定伪善的道德内涵提供了具有历史厚度的学理渊源。也就是在这样的西方古典伦理学视角下,伪善的本义即"似善非善"便浮出"历史水面"。

关键词：伪善西方古典伦理学似善非善

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德性伦理应用于企业的合理性及其建构

华中科技大学学报（社会科学版） 2015年第2期29卷 30-35页

作者：闫茂伟东南大学人文学院江苏南京211189

在西方,德性伦理的应用性早已呈现在相关的理论和实践中,并为当今德性伦理应用于企业提供了基本的理论依据和实践旨向。而德性伦理的独特立场、明显优势以及作为企业解决现实道德难题的伦理资源为德性伦理应用于企业提供了一定的现实合... 详细信息

在西方,德性伦理的应用性早已呈现在相关的理论和实践中,并为当今德性伦理应用于企业提供了基本的理论依据和实践旨向。而德性伦理的独特立场、明显优势以及作为企业解决现实道德难题的伦理资源为德性伦理应用于企业提供了一定的现实合理性。为此,惟有建构目的善、品质善和实体善有机统一的德性伦理体系和利益与责任相关者共同参与的公司治理体系,德性伦理应用于企业的合理性方能得以建构,进而才能使得企业以德性为其自身以及活动的本源。

关键词：德性伦理企业应用性合理性

Asymmetric interfacial engineering regulated charge distribution on Ru/Ni-N-C sites for efficient hydrogen evolution reaction in alkaline and seawater electrolytes

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Science China Chemistry 2025年

作者： Rui Yu Xi Cao Chengyang Zhu Qingqing Chen Ziyi yan Binbin Jiang Junjie mao Xianwen wei Key Laboratory of Electrochemical Clean Energy of Anhui Higher Education Institutes College of Chemistry and Materials ScienceAnhui Normal University Anhui Provincial Key Laboratory of Advanced Catalysis and Energy Materials School of Chemistry and Chemical Engineering Anqing Normal University School of Chemistry and Chemical Engineering Anhui University of Technology

Developing highly efficient and cost-effective electrocatalysts for hydrogen evolution reaction（HER） is grandly challenging in alkaline and alkaline seawater electrolytes. Herein, the asymmetric interfacial active sites with Ru nanoparticles and Ni single atoms in Ru/Ni-N-C were designed through a solvothermal-pyrolysis process. Constructing highly asymmetric interfacial active sites with a strong electron delocalization effect significantly improves HER kinetics. As expected, Ru/Ni-N-C requires low overpotentials with around 20 mV at 10 mA cm-2and robust durability over 48 h in alkaline and alkaline seawater *** at 200 mA cm-2, the overpotentials are close to 100 mV in both above electrolytes for Ru/Ni-N-C. Density function theory calculation results suggest that the asymmetric interface between Ru nanoparticle and Ni-N-C could accelerate the dissociation of water molecules and modulate the hydrogen species, consequently improving the HER performances. This work opens a unique approach for constructing advanced materials for energy conversion.

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High Fe‑Loading Single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

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Nano-Micro Letters 2025年第2期17卷 187-203页

作者： Si Chen Fang Huang Lijie mao Zhimin Zhang Han Lin Qixin yan Xiangyu Lu Jianlin Shi Department of Cardiology Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200092People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China Shanghai Frontiers Science Center of Nanocatalytic Medicine School of MedicineTongji UniversityShanghai 200092People’s Republic of China

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site ***,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with *** constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial ***,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading ***,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/*** addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)*** in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound *** work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

关键词： Nanocatalytic medicine Single-atom catalysts Reactive oxygen species(ROS) High metal loading Oxidase catalysis

Solar cycles during the seventeenth century revealed by equatorial aurora records

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Earth and Planetary Physics 2025年第1期9卷 182-187页

作者： Yong wei LiMei yan Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

Solar cycles are fundamental to astrophysics,space exploration,technological infrastructure,and Earth's climate.A better understanding of these cycles and their history can aid in risk mitigation on Earth,while also deepening our knowledge of stellar physics and solar system *** the solar cycles between 1600 and 1700-especially the post-1645 Maunder Minimum,characterized by significantly reduced solar activity-poses challenges to existing solar activity *** study utilizes a new red equatorial auroral catalog from ancient Korean texts to establish solar cycle patterns from 1623 to ***,a further reevaluation of the solar cycles between 1610 and 1755 identified a total of 13 cycles,diverging from the widely accepted record of 12 cycles during that *** research enhances our understanding of historical solar activity,and underscores the importance of integrating diverse historical sources into modern analyses.

关键词： solar cycle Maunder Minimum solar activity red equatorial aurora West Pacific geomagnetic anomaly

Construction of hpaA gene from a clinical isolate of Helicobacter pyloriand identification of fusion protein

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World Journal of Gastroenterology 2003年第7期9卷 1529-1536页

作者： Ya-Fei mao Jie yan Li-wei Li Shu-Ping Li Department of Medical Microbiology and Parasitology,College of Medical Sciences,Zhejiang University,Hangzhou 310031,Zhejiang Province,China Department of Medical Microbiology and Parasitology College of Medical Sciences Zhejiang University Hangzhou 310031 Zhejiang Province China.

AIM: To clone hpaA gene from a clinical strain of Helicobacter pylori and to construct the expression vector of the gene and to identify immunity of the fusion ***: The hpaA gene from a clinical isolate Y06 of *** was amplified by high fidelity PCR. The nucleotide sequence of the target DNA amplification fragment was sequenced after T-A cloning. The recombinant expression vector inserted with hpaA gene was constructed. The expression of HpaA fusion protein in E. coli BL21DE3 induced by IPTG at different dosages was examined by *** blot with commercial antibody against whole cell of H. pylorias well as immunodiffusion assay with selfprepared rabbit antiserum against HpaA fusion protein were applied to determine immunity of the fusion protein. ELISA was used to detect the antibody against HpaA in sera of 125 patients infected with H. pylori and to examine HpaA expression of 109 clinical isolates of H. ***: In comparison with the reported corresponding sequences, the homologies of nucleotide and putative amino acid sequences of the cloned hpaA gene were from 94.25-97.32 % and 95.38-98.46 %, respectively. The output of HpaA fusion protein in its expression system of pET32ahpaA-BL21DE3 was approximately 40 % of the total bacterial proteins. HpaA fusion protein was able to combine with the commercial antibody against whole cell of H. pyloriand to induce rabbit producing specific antiserum with 1:4immunodiffusion titer after the animal was immunized with the fusion protein. 81.6 % of the serum samples from 125patients infected with ***(102/125) were positive for HpaA antibody and all of the tested isolates of ***(109/109) were detectable for ***: A prokaryotic expression system with high efficiency of *** gene was successfully *** HpaA expressing fusion protein showed satisfactory immunoreactivity and antigenicity. High frequencies of HpaA expression in different H. pyloriclinicalstrains an

关键词： hpaA基因幽门螺旋杆菌融合蛋白聚合酶链反应

Analysis of Micromechanical Properties at the Interface of Pre-wet SBS Modified Asphalt Mixture Based on Molecular Simulation Technology

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 103-113页

作者： CHEN Wuxing CHEN Shuang YU yan ZHANG Jiangyi XU Haiyang GUO wei School of Mines China University of Mining and TechnologyXuzhou 221000China College of Construction Engineering Jilin Uni-versityChangchun 130026China

The pre-wetting of aggregate surface is a means to improve the interface performance of SBS modified asphalt and *** effect of pre-wetting technology on the interaction between SBS modified asphalt and aggregate was analyzed by molecular dynamics *** diffusion coefficient and concentration distribution of SBS modified asphalt on aggregate surface are *** simulation results show that the diffusion coefficient of the aggregate surface of SBS modified asphalt is increased by 47.6%and 70.5%respectively after 110#asphalt and 130#asphalt are *** concentration distribution of SBS modified asphalt on the aggregate surface after pre-wetting is more *** to the results of interface energy calculation,the interface energy of SBS modified bitumen and aggregate can be increased by about 5%after *** to the results of molecular dynamics simulation,the pre-wetting technology can effectively improve the interface workability of SBS modified bitumen and aggregate,so as to improve the interface performance.

关键词： pre-wet oil-stone interface interface interaction interface mechanics molecular dynamics simulation