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Applied mathematics and mechanics(English Edition) 2020年第4期41卷 637-654页

作者： Lijun ZHANG m.B.ARAIN m.m.BHATTI A.ZEESHAN H.hal-SULAmI College of mathematics and Systems Science Shandong University of Science and Technology Nonlinear Analysis and Applied mathematics (NAAm)-Research Group Department of Mathematics Faculty of Science King Abdulaziz University Department of mathematics and Statistics International Islamic University

The three-dimensional(3D) nano?uid ?ow among the rotating circular plates ?lled with nanoparticles and gyrotactic microorganisms is studied. A generalized form of the magnetic Reynolds number is used for the mathematical modeling of the ferro-nano?uid ?ow. The torque e?ects on the lower and upper plates are calculated.A di?erential transform scheme with the Pad′e approximation is used to solve the coupled highly nonlinear ordinary di?erential equations. The results show that the squeeze Reynolds number signi?cantly suppresses the temperature, microorganism, and nanoparticle concentration distribution, and agree well with those obtained by the numerical method.

关键词： swimming of microorganism generalized magnetic Reynolds number nanofluid differential transform solution circular rotating plate

Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in ***

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Synthetic and Systems Biotechnology 2024年第2期9卷 330-339页

作者： Kristin V.Presnell Omar melhem Sarah m.Coleman Nicholas J.morse hal S.Alper mcKetta Department of Chemical Engineering The University of Texas at Austin200E Dean Keeton St.Stop C0400AustinTX78712USA Institute for Cellular and molecular Biology The University of Texas at Austin2500 Speedway AvenueAustinTX78712USA

metabolic engineering and synthetic biology endeavors benefit from promoters that perform consistently(or robustly)with respect to cellular growth phase(exponential and stationary)and fermentation scale(microtiter plates,tubes,flasks,and bioreactors).However,nearly all endogenous promoters(especially in Saccharomyces cerevisiae)do not perform in this *** this work,a hybrid promoter engineering strategy is leveraged to create novel synthetic promoters with robustness across these *** a multi-dimensional RNA-seq dataset,promoters with specific phase dependencies were *** enriched with functional transcription factors were identified using mEmE *** motif-containing fragments could impart activity dependence in the opposing ***,we obtain two new promoters with high and consistent expression across both phases by increasing the exponential phase activity of the starting stationary-phase scaffold by 38 and 23-fold ***,we show that these promoters function consistently across various laboratory growth scales over time in a microtiter plate and in ***,this work presents and validates a new strategy for engineering promoters in *** with high levels of expression that are robust to cellular growth phase and the scale of the culture.

关键词： endogenous synthetic stationary

NEW ERROR ESTImATES FOR LINEAR TRIANGLE FINITE ELEmENTS IN THE STEKLOV EIGENVALUE PROBLEm

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Journal of Computational mathematics 2018年第5期36卷 682-692页

作者： hal Bi Yidu Yang Yuanyuan Yu Jiayu Han School of mathematical Sciences Guizhou Normal University Guiyang 550001 China

This paper is concerned with the finite elements approximation for the Steklov eigen- value problem on concave polygonal domain. We make full use of the regularity estimate and the characteristic of edge average interpolation operator of nonconforming Crouzeix- Raviart element, and prove a new and optimal error estimate in ｜｜｜｜o,δΩ for the eigenfunc- tion of linear conforming finite element and the nonconforming Crouzeix-Raviart element. Finally, we present some numerical results to support the theoretical analysis.

关键词： Steklov eigenvalue problem Concave polygonal domain Linear conforming finite element Nonconforming Crouzeix-Raviart element Error estimates.

火花源光发射光谱法分析中测量不确定度的表述及原材料规格一致性概率的计算

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冶金分析 2012年第1期32卷 19-22页

作者： Edmund halász J-m Bhlen 赛默飞世尔科技有限公司

根据ISO 17025,每年有越来越多的实验室开始使用光发射光谱仪(OES)。ISO17025的遵守提升了评估和报道测量不确定度的重要性。《测量不确定度表示指南》(GUm)提供了不确定度分析的指导方法。不过,这些指导方针的实施是一项艰巨的任务,尤... 详细信息

根据ISO 17025,每年有越来越多的实验室开始使用光发射光谱仪(OES)。ISO17025的遵守提升了评估和报道测量不确定度的重要性。《测量不确定度表示指南》(GUm)提供了不确定度分析的指导方法。不过,这些指导方针的实施是一项艰巨的任务,尤其是不熟悉必要的数学和统计学概念时。因此,试验和校准人员必须经常寻找现成的工具以满足分析需求。本文报道了赛默飞世尔科技有限公司实施的分析软件,用于测量不确定度的计算。此外,还描述了基于不确定度知识的应用,以用于计算产品一致性的概率。

关键词：火花源光发射光谱测量不确定度置信区间一致性测试风险分析

Transporting droplets through surface anisotropy

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microsystems & Nanoengineering 2015年第1期1卷 74-81页

作者： hal R.Holmes Karl F.Böhringer Department of Bioengineering University of WashingtonSeattleWA 98195USA Department of Electrical Engineering University of WashingtonSeattleWA 98195USA

This review article examines digital microfluidic systems that manipulate droplets through surface *** systems are categorized as surface tension driven or contact line *** tension driven systems include electrowetting on dielectric,marangoni flow on microheater arrays,and chemical gradient surfaces,whereas contact line driven systems include anisotropic ratchet conveyors,nanostructured Parylene ratchets,and tilted pillar *** article describes the operating principles and outlines the fabrication procedures for each *** also present new equations that unify several previous models of contact line driven *** strengths and weaknesses of each system are compared,with a focus on their ability to perform the generation,switching,fusion,and fission of ***,we discuss current and potential future applications of these systems.

关键词： droplets digital microfluidics electrowetting thermocapillary forces contact line oscillation

Bioproduced Proteins On Demand(Bio-POD)in hydrogels using Pichia pastoris

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Bioactive materials 2021年第8期6卷 2390-2399页

作者： Shuo-Fu Yuan Sierra m.Brooks Annalee W.Nguyen Wen-Ling Lin Trevor G.Johnston Jennifer A.maynard Alshakim Nelson hal S.Alper Institute for Cellular and molecular Biology The University of Texas at AustinAustinTXUSA mcKetta Department of Chemical Engineering The University of Texas at AustinAustinTXUSA Department of Chemistry University of WashingtonBox 351700SeattleWAUSA

Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation *** a result,these systems are not easily portable or reusable for on-demand protein production *** this study,we employ Bioproduced Proteins On Demand(Bio-POD),a F127-bisurethane methacrylate hydrogel-based technique that immobilizes engineered Pichia pastoris for preservable,on-demand production and secretion of medium-and high-molecular weight proteins(in this case,SEAP,α-amylase,and anti-HER2).The gel samples containing encapsulated-yeast demonstrated sustained protein production and exhibited productivity immediately after lyophilization and *** hydrogel platform described here is the first hydrogel immobilization using a *** system to produce recombinant proteins of this *** results highlight the potential of this formulation to establish a cost-effective bioprocessing strategy for on-demand protein production.

关键词： Protein production Hydrogel Pichia pastoris Immobilization Lyophilization

Nelson Textbook of Pediatrics

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Food plant with antioxidant,tyrosinase inhibitory and antimelanoma activity:Prunus mahaleb L

2004年

作者： Richard E., Behrman , Robert m., Kliegman , hal B. Jenson

来源：畅想之星图书光盘版（南... 评论

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Food Bioscience 2022年第4期48卷 464-473页

作者： Zühal Bayrakçeken Güven Zeynep Dogan Iclal Saracoglu Laurent Picot Akito Nagatsu A.Ahmet Basaran Hacettepe University Faculty of PharmacyDepartment of PharmacognosyAnkaraTurkey La Rochelle University UMR CNRS 7266 LIENSsLa RochelleFrance Kinjo Gakuin University College of PharmacyDepartment of PharmacognosyNagoyaJapan Baskent University Faculty of PharmacyDepartment of PharmacognosyAnkaraTurkey Erzincan Binali Yıldırım University Faculty of PharmacyDepartment of PharmacognosyErzincanTurkey

Prunus mahaleb *** are used as spice and folk remedies in many *** aim of this study was bioactivity guided isolation and characterization phytoconstituents of *** which have been studied very limited so *** antioxidant capacities of seed and kernel(endocarp)extracts were studied by DPPH,nitric oxide(NO),superoxide(SO),TEAC,CUPRAC *** antiproliferative activity were investigated for the first time against B16F10,A2058,HeLa and L929 cell lines using mTT *** acid and ursolic acid which have strong antioxidant and cell growth inhibitory effect,were isolated from the most active *** the inactive fractions,quercetin-3-O-(2-O,6-O-α-dirhamnopyranosylβ-glucopyranoside),kaempferol-3-O-(2-O,6-O-α-dirhamnopyranosylβ-glucopyranoside)andβ-sitosterol 3-O-β-glucopyranoside were *** study is the first record for the isolation of ursolic acid from all parts of ***,gallic acid from *** kernels,two flavonol triglycosides from the Prunus species and their analysis by advanced NmR spectroscopic *** acid(IC50:170.2μm)and gallic acid(IC50:10.5μm)showed strong tyrosinase inhibition,gallic acid being 5.4 times more effective than standard *** extracts have strong antioxidant activity,showed high growth inhibitory activity with dose-dependent manner on melanoma cells but no growth inhibitory on healthy L929 and HeLa cancer *** high antioxidant capacity of kernel,its selective growth inhibition of B16F10 and A2058 melanoma cells and its strong tyrosinase inhibitory compounds suggest that it could be a new,non-toxic and inexpensive resource for melanoma treatment and cosmetic industry.

关键词： Prunus mahaleb L. Chemical composition Cell growth inhibition Antioxidant Tyrosinase inhibition

The angiotensin Ⅱ type 1 receptor and receptor-associated proteins

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Cell Research 2001年第3期11卷 165-180页

作者： GUO DENG FU, YU LIN SUN, PAVEL HAmET, TADASHI INAGAmI Research Centre, Hotel-Dieu of chum and Department of medicine, University of montreal, montreal, Quebec, Canada H2W 1T8 Department of Biochemistry, Vanderbilt University, School of medicine, Nashville Research Centre Hotel-Dieu of CHUM and Department of Medicine University of Montreal Montreal Quebec Canada H2W 1T8. Department of Biochemistry Vanderbilt University School of Medicine Nashville TN USA 37232.

The mechanisms of regulation, activation and signal transduction of the angiotensin II (Ang II) type 1 (AT1) receptor have been studied extensively in the decade after its cloning. The AT1 receptor is a major component of the renin-angiotensin system (RAS). It mediates the classical biological actions of Ang II. Among the structures required for regulation and activation of the receptor, its carboxyl- terminal region plays crucial roles in receptor internalization, desensitization and phosphorylation. The mechanisms involved in heterotrimeric G-protein coupling to the receptor, activation of the downstream signaling pathway by G proteins and the Ang II signal transduction pathways leading to specific cellular responses are discussed. In addition, recent work on the identification and characterization of novel proteins associated with carboxy1-terminus of the AT1 receptor is presented. These novel proteins will advance our understanding of how the receptor is internalized and recycled as they provide molecular mechanisms for the activation and regulation of G-protein-coupled receptors.

关键词： RAS Ang II receptor internalization recycling yeast two-hybrid system.

火花源光发射光谱法分析钢中夹杂物的研究进展——夹杂物成分和尺寸的现象学分析与计算

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冶金分析 2011年第10期31卷 7-13页

作者： Edmund halász Jean-Luc Dorier Jean-marc Bhlen 赛默飞世尔科技有限公司

为更好了解与试样电火花烧蚀有关的实验现象,我们设计了一种新算法,并报道了其用于测定夹杂物尺寸的结果。在此算法中,用元素氧化物、硫化物和氮化物测量通道的单火花强度分析技术测定了某一特定类型夹杂物中各元素的浓度,包括铝、钙、... 详细信息

为更好了解与试样电火花烧蚀有关的实验现象,我们设计了一种新算法,并报道了其用于测定夹杂物尺寸的结果。在此算法中,用元素氧化物、硫化物和氮化物测量通道的单火花强度分析技术测定了某一特定类型夹杂物中各元素的浓度,包括铝、钙、镁、锰、钛以及硅等。对每个相关通道,计算了指定类型夹杂物有关的强度峰值对应的强度值之和与所有强度值和之间的比值。在光谱化学分析中,此比值与测试浓度相乘,所得结果为指定类型夹杂物中元素的平均浓度。结合单火花烧蚀质量与夹杂物成分的化学计量学分析,可以估算夹杂物的尺寸。通过进一步的分析可以实现夹杂物尺寸(小、中和大级别)的辨别。本方法的创新性在于,单火花源强度既不需要漂移修正也不需要校正。本算法可以直接用于计算钢中大部分常见夹杂物的平均浓度和尺寸的分析,例如氧化物、硫化物和尖晶石。测定结果显示夹杂物的平均直径在1～10μm之间。

关键词：光发射光谱火花源光发射光谱-脉冲差别分析法非金属夹杂物夹杂物分析夹杂物成分夹杂物尺寸