检索结果-南通市图书馆

Encapsulation of docosahexaenoic acid (DHA) using self-assembling food-derived proteins for efficient biological functions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2023年第5期12卷 1861-1871页

作者： Yumeng Liu Kaixin Cao Tong Li Delun mu Naiqi Zhang Ying Wang rina Wu Junrui Wu College of Food Science Shenyang Agricultural UniversityShenyang 110866China Liaoning Engineering Research Center of Food Fermentation Technology Shenyang 110161China Shenyang Key Laboratory of microbial Fermentation Technology Innovation Shenyang 110866China

Docosahexaenoic acid(DHA;22n-6)possesses multiple biological functions, including antioxidant activity and ameliorating hypertriglyceridemia. However, the application of DHA has been limited due to poor aqueous solubility and susceptible to oxidation. Here, ovalbumin(O), myosin(m), 7S soy globulin(S), and β-lactoglobulin(β), hydrolyzed by chymotrypsin, self-assembled into micelles, respectively. Adding incremental DHA to micelles caused endogenous fluorescence quenching of O, m, S, and β micelles, implying successful incorporation of DHA into hydrophobic cores of micelles(O(DHA), m(DHA), S(DHA), and β(DHA)). The results showed that micelles provided spatial stability and improved solubility, and stability against thermal and ultraviolet(UV)light for DHA. The uptake of DHA from m(DHA), β(DHA), O(DHA), and S(DHA)was 3.27-, 3.91-, 2.7-, and 3.95-fold higher, respectively, than that of DHA by Caco-2 cells. Encapsulation in micelles increased DHA aqueous solubility and uptake, which enhanced cellular endogenous antioxidant defense. meanwhile, increased uptake of DHA was verified by HepG2 cells, and O, m, S, and β micelles were proven to increase DHA uptake to reduce lipid deposition. Our findings strongly support the possibility that O, m, S, and β micelles can be regarded as a carrier for loading DHA.

关键词： Docosahexaenoic acid micelle Solubility Stability Cellular uptake

Climate change evidence in tree growth and stand productivity at the upper treeline ecotone in the Polar Ural mountains

在线全文

学校读者我要写书评

暂无评论

Forest Ecosystems 2020年第1期7卷 76-91页

作者： Nadezhda m.Devi Vladimir V.Kukarskih Аrina A.Galimova Valeriy S.mazepa Andrey A.Grigoriev Institute of Plant and Animal Ecology Ural BranchRussian Academy of Sciences8 Marta202/3Ekaterinburg 620144Russia

Background:Recent warming is affecting species composition and species areal distribution of many ***,although most treeline studies have estimated the rates of forest expansion into tundra,still little is known about the long-term dynamic of stand productivity at the forest-tundra ***,we make use of tree-ring data from 350 larch(Larix sibirica Ledeb.)and spruce(Picea obovata Ledeb.)sampled along the singular altitudinal treeline ecotone at the Polar Urals to assess the dynamic of stand establishment and productivity,and link the results with meteorological observations to identify the main environmental ***:The analysis of stand instalment indicated that more than 90%of the living trees appeared after *** this period,the stand became denser and moved 50m upward,while in recent decades the trees of both species grew *** maximum afforestation occurred in the last decades of the twentieth century,and the large number of encountered saplings indicates that the forest is still *** upward shift coincided with a slight increase of may-August and nearly doubling of September-April precipitation while the increase in growth matched with an early growth season warming(June+0.27°C per decade since 1901).This increase in radial growth combined with the stand densification led to a 6-90 times increase of biomass since ***:Tree-ring based twentieth century reconstruction at the treeline ecotone shows an ongoing forest densification and expansion accompanied by an increased *** changes are driven by climate change mechanism,whereby the leading factors are the significant increase in may-June temperatures and precipitation during the dormant *** of phytomass accumulation mechanisms within treeline ecotone is valuable for improving our understanding of carbon dynamics and the overall climate balance in current treeline ecosystems and for predicting how these will be altered by global ch

关键词： Polar Urals Treeline Productivity Climate change Radial growth Recruitment Larch Spruce

在线全文

学校读者我要写书评

暂无评论

向列相液晶器件光调制性能研究

沈阳理工大学学报 2024年第1期43卷 91-96页

作者：王萧鲁小鑫张雪凤王浪乌日娜沈阳理工大学理学院沈阳110159

液晶的折射率易受外加电压和温度的影响,据此可获得电场和温度可调制器件。为改善液晶器件对光的调制性能,制备了向列相液晶器件,并对其调制性能进行理论模拟和实验研究。首先研究液晶器件的透过光强和相位延迟随电压变化关系,结果显示... 详细信息

液晶的折射率易受外加电压和温度的影响,据此可获得电场和温度可调制器件。为改善液晶器件对光的调制性能,制备了向列相液晶器件,并对其调制性能进行理论模拟和实验研究。首先研究液晶器件的透过光强和相位延迟随电压变化关系,结果显示,相同电压下,增大器件厚度,光强调制曲线的峰谷数增多,相位调制量增大,其中电压在0.8~3 V范围内,相位延迟曲线变化较明显,厚度为4、6和10μm的器件可分别获得约1.21π、1.87π和3.19πrad的相位调制量。其次对厚度为4μm的器件进行温度可调制性能的研究,结果显示,温度从20℃升至45℃时,获得的温度可调制量约为0.29πrad。改变电场或温度均能引起液晶的折射率变化,从而实现对光的调控,适当增加液晶器件厚度可获得较大的相位调制量。研究结果对液晶光调制器件的性能改善及应用具有重要参考意义。

关键词：液晶光调制器向列相液晶光强调制相位调制

基于随机森林的川崎病诊断关键实验室指标预测研究

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

检验医学与临床 2024年第19期21卷 2887-2891,2897页

作者：王爱琼萨日娜张巧丽柴利娜李爱月内蒙古自治区鄂尔多斯市中心医院儿科内蒙古鄂尔多斯017000 内蒙古迪安丰信医疗科技有限责任公司内蒙古呼和浩特010000

目的通过随机森林算法构建诊断模型,分析不完全川崎病区别于完全川崎病的关键实验室指标,以提高不完全川崎病的诊断率及降低其误诊率。方法选取2018年1月至2023年6月就诊于鄂尔多斯市中心医院儿科确诊为完全川崎病或不完全川崎病的患儿... 详细信息

目的通过随机森林算法构建诊断模型,分析不完全川崎病区别于完全川崎病的关键实验室指标,以提高不完全川崎病的诊断率及降低其误诊率。方法选取2018年1月至2023年6月就诊于鄂尔多斯市中心医院儿科确诊为完全川崎病或不完全川崎病的患儿145例,其中不完全川崎病34例(不完全组),完全川崎病111例(完全组)。比较两组红细胞计数、血红蛋白、红细胞沉降率、血钠、白细胞计数、血小板计数、平均血小板体积、中性粒细胞计数、中性粒细胞百分比、淋巴细胞计数、淋巴细胞百分比、C反应蛋白、丙氨酸转氨酶、总蛋白、清蛋白、总胆红素、天冬氨酸转氨酶、降钙素原(PCT)、肌酸激酶、肌酸激酶m多肽(CK-m)、N末端B型钠尿肽前体(NT-proBNP)、凝血酶原时间、凝血酶时间、D-二聚体、尿白细胞计数的差异,利用随机森林算法构建诊断模型,分析不完全川崎病区别于完全川崎病的实验室指标。结果模型结果显示,贡献度前5位的实验室指标依次为血小板计数(0.381)、尿白细胞计数(0.372)、淋巴细胞百分比(0.260)、凝血酶时间(0.255)及中性粒细胞百分比(0.163),在区分川崎病类型方面具有重要的指示意义。构建的诊断模型ROC曲线下面积为0.864,灵敏度为1.00,特异度为0.74。结论通过随机森林算法得出,血小板计数、尿白细胞计数、淋巴细胞百分比、凝血酶时间以及中性粒细胞百分比可作为诊断不完全川崎病的关键实验室指标,这为患儿的诊断与及时治疗提供理论依据。

关键词：随机森林不完全川崎病实验室指标早期诊断完全川崎病

维普期刊数据库博看期刊

Variability in the Levels of BTEX at a Pollution Hotspot in New Delhi, India

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2016年第10期7卷 1245-1258页

作者： manisha Gaur rina Singh Anuradha Shukla Academy of Scientific & Innovative Research (AcSIR) Environmental Science Division CSIR-Central Road Research Institute (CRRI) New Delhi India Environmental Science Division CSIR-Central Road Research Institute (CRRI) New Delhi India

It is a matter of concern that despite taking measures to control aromatic content in gasoline in India, the levels of volatile organic compounds are rising again in many of the Indian cities. The transport sector has been one of the major sectors which are re-sponsible for worsening the air quality of Delhi city. While thousands of toxic com-pounds are emitted from automobiles, industries, gasoline stations as well as service stations, however, volatile organic compounds are important due to their significant contribution to ozone formation, cancer, and non-cancer health risks. In this study, roadside levels of benzene, toluene, ethylbenzene and xylenes (BTEX) were investi-gated at the one of the major arterial road of Delhi city. Air samples were collected by activated coconut shell charcoal adsorbent tubes. The BTEX concentrations were de-termined by gas chromatography-flame ionization detector (GC-FID) technique. The mean concentrations of benzene, toluene, ethylbenzene and xylenes were, respectively, 60.22, 162.68, 49.42 and 25.25 μg/m3. The relative concentration distribution pattern and mutual correlation analysis indicated that in BTEX had sources other than vehicle emission at the study site. The samples collected, showed that BTEX had significantly higher concentrations in winter than those in spring and summer.

关键词： BTEX Urban Air Pollution Vehicle Emissions Delhi

On Pairwise Singular Sets and Pairwise Singular maps

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of mathematics and System Science 2014年第4期4卷 261-268页

作者： rina Pankaj Verma mamta Singh School of Studies in mathematics Vikram University Ujjain (M.P.) India Department of Appl. Sci. & Comp. Tech. Bundelkhand University Jhansi India

In this paper we introduce the concept of pairwise singular sets and pairwise singular maps between pairwise locally compact and pairwise hausdorff spaces and study the properties of pairwise singular maps.

关键词： Pairwise singular sets pairwise singular maps

Seasonal and Spatial Variation of BTEX in Ambient Air of Delhi

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2016年第5期7卷 670-688页

作者： rina Singh manisha Gaur Anuradha Shukla Environmental Science Division CSIR-CRRI New Delhi India Environmental Science Division AcSIR-CRRI New Delhi India

The present study aims to provide an indication of ambient levels of BTEX in the immediate vicinity of petrol filling stations in Delhi & other road side corridors of Delhi. In this study spatial and seasonal variation of BTEX has been measured at different locations in Delhi. For the measurement of VOCs, passive based diffusion methodology was adopted for the adsorption of BTEX over the activated charcoal. Four categories of sampling sites were chosen (residential, traffic junctions, road side corridors and petrol pumps). The result confirms that the aromatic concentrations in the ambient air of gas stations are appreciably higher than the average values in locations with high vehicular flux. The mean concentration of BTEX at all the locations was 0.622μg/m3, 0.361 μg/m3, 1.037 μg/m3 and 0.122 μg/m3. The average concentration of benzene was highest at petrol pump 3.5 μg/m3 however at all other sampling sites it was in the range of 0.294 to 0.712 μg/m3. The mean concentration value shows that the xylene concentration dominates more in Delhi as compared to benzene. The total average BTEX concentration at road side corridors was 0.594 μg.m-3, at residential corridors was 0.262 μg.m-3, at intersections it was 0.649 μg.m-3 and at petrol filling stations it was 1.457 μg.m-3. The concentration was in the order of petrol filling stations > intersections > road side corridors > residence. These data show the predominance of diesel driven vehicles in Delhi since xylene mainly comes from diesel exhausts. The influential VOC species in case of diesel engine exhaust are benzene, ethylbenzene, xylenes, propane, n-decane and undecane and in case of gasoline vehicle exhaust influential species are benzene, ethylbenzene, 1,2,4-methylbenzene, 1,3,5-methylbenzene, n-hexane and 2-methylhexane. So xylene is mainly coming from diesel exhaust since gasoline vehicle exhaust doesn’t show this

关键词： VOCs Benzene Toluene Xylene Ethylbenzene

健脾五味丸HPLC指纹图谱的建立及多指标成分的含量测定研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

现代中药研究与实践 2024年第3期38卷 47-51页

作者：张玉婷莎日娜王焕芸何春龙张屏孙丽君内蒙古医科大学药学院内蒙古呼和浩特010110 内蒙古医科大学第二附属医院内蒙古呼和浩特手010110

目的建立蒙药健脾五味丸指纹图谱和多指标含量测定方法,为其质量控制提供依据。方法采用Sepax HP-C_(18)(4.6 mm × 250 mm,5 μm)色谱柱,乙腈-0.1%甲酸溶液为流动相,梯度洗脱,流速为1 mL/min,检测波长为254 nm,柱温为28 ℃,进样量为... 详细信息

目的建立蒙药健脾五味丸指纹图谱和多指标含量测定方法,为其质量控制提供依据。方法采用Sepax HP-C_(18)(4.6 mm × 250 mm,5 μm)色谱柱,乙腈-0.1%甲酸溶液为流动相,梯度洗脱,流速为1 mL/min,检测波长为254 nm,柱温为28 ℃,进样量为10 μL;建立10 批健脾五味丸的指纹图谱,并采用一测多评法测定其中7种成分的含量。结果健脾五味丸HPLC指纹图谱确定了11个共有峰,指认出原儿茶酸、没食子酸、胡黄连苷Ⅰ、胡黄连苷Ⅱ、鞣花酸、土木香内酯和异土木香内酯7个成分,通过一测多评法计算得到7个成分的含量值,与外标法比较10 批样品含量测定结果一致。经方法学验证,线性关系良好(r ≥ 0.999),加样回收率为96.68% ~ 104.44%。结论所建立的指纹图谱和多指标含量测定方法准确、高效、特征性强,可为健脾五味丸的质量评价和临床应用提供参考。

关键词：健脾五味丸指纹图谱一测多评法高效液相色谱法

基于青色荧光蛋白和FAm标记核酸的荧光能量共振转移分析PUF蛋白与其目标核酸的互作

在线全文

学校读者我要写书评

暂无评论

基于青色荧光蛋白和FAM标记核酸的荧光能量共振转移分析PUF蛋白与...

作者： Fidiniaina rina Juliana 浙江师范大学

学位级别：硕士

在细胞内,各种生物分子之间的相互作用,对于调节、维持细胞的正常生长、发育、繁殖具有非常重要的意义。F?rster或荧光共振能量转移(fluorescence resonance energy transfer,FRET)是一种物理现象,需要在一对分子之间发生非辐射能量转移... 详细信息

在细胞内,各种生物分子之间的相互作用,对于调节、维持细胞的正常生长、发育、繁殖具有非常重要的意义。F?rster或荧光共振能量转移(fluorescence resonance energy transfer,FRET)是一种物理现象,需要在一对分子之间发生非辐射能量转移,这取决于距离以及光子从供体到受体荧光色团的发射和再吸收。这种能量转移的效率高度依赖于供体和受体荧光团的选择,荧光蛋白是最流行的荧光色团之一,可以与不同的蛋白质分子形成融合蛋白,有机荧光色团可用来标记目标核酸序列。FRET技术可以用来分析蛋白质之间相互作用,以及蛋白质与其他分子之间的相互作用。PUF蛋白家族是一种高度保守的蛋白,属于广泛的真核细胞RNA结合蛋白家族,它们通过与靶标m RNA的3’非翻译区(UTR)结合并调节其降解来调节靶标m RNA的生命周期。不同的PUF蛋白具有称为RNA结合支架或PUF蛋白结构域的特异性结合位点,可调节靶RNA的表达。在长期进化过程中,PUF蛋白的结合特异性,调控和靶标等在真核生物中出现了多样化现象。科学家已经分析出PUF蛋白氨基酸序列与识别核苷酸序列的对应关系,为人工设计PUF蛋白并应用于识别结合特定RNA序列奠定了基础。在本研究中,我们首先通过绿色荧光蛋白序列基因的64、65、66和72位的定点突变设计了一种新的蓝绿色荧光蛋白mutCFP,并分析了其发射光谱。此mutCFP在490nm波长处产生一个单峰发射光谱,这与普通青色荧光蛋白在476nm和500nm波长处具有双峰发射光谱有所不同,mutCFP可作为FAm荧光色团FRET的荧光供体。构建PUF3-mutCFP融合蛋白表达载体,并纯化融合蛋白,分析其与目标核苷酸的互作。PUF3蛋白可以结合12个寡核苷酸(GATCGGCAGCGA),我们在其编码序列的3’端连接mutCFP编码序列,并在大肠杆菌胞内表达此融合蛋白。经分离、纯化获得此融合蛋白PUF3-mutCFP。我们设计、合成在6-FAm标记与PUF识别位点之间有不同长度腺苷酸的寡核苷酸链,并以融合蛋白PUF3-mutCFP中的mutCFP作为荧光供体,6-FAm作为荧光受体进行FRET分析。结果我们发现与15、20和25个腺苷酸相比,当FAm和靶序列之间有9个连接腺苷酸时,PUF3与目标寡核苷酸具有最高亲和力(K=163.3 nm)。而与6、8和10个寡核苷酸相比,PUF3对12个寡核苷酸靶序列具有最高亲和力(K=44.3 nm)。用FRET技术进一步研究了pH,甘油和还原剂对PUF3和目标寡核苷酸链互作的影响。通过解离常数测定分析,发现PUF3和目标寡核苷酸链的亲和力受到这些因素的影响,且在pH8,20%甘油和2 m m DTT的条件下具有更强的亲和力。本研究利用FRET技术分析PUF3与目标寡核苷酸链的互作条件,如6-FAm标记与PUF识别位点之间间隔核苷酸链长度的影响,并评估不同pH、甘油和还原剂等对其相互作用的潜在影响。总之,本研究建立了一个RNA结合蛋白与目标核苷酸链互作的互作分析体系,为体外分析其生物大分子之间的互作、深入探究它们体内的互作功能建立的一定基础。

关键词：荧光共振能量转移 RNA结合蛋白蛋白核酸互作青色荧光蛋白 PUF蛋白