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A step-by-step protocol for formaldehyde-assisted isolation of regulatory elements from Arabidopsis thaliana

Journal of Integrative Plant Biology 2014年第6期56卷 527-538页

作者： mohammad Amin Omidbakhshfard Flavia Vischi Winck Samuel Arvidsson Diego m.ria?o-Pachón Bernd mueller-Roeber University of Potsdam Institute of Biochemistry and Biology Potsdam-Golm 14476 Germany max-Planck Institute of molecular Plant Physiology Potsdam-Golm 14476 Germany Brazilian Bioethanol Science and Technology Laboratory (CTBE) Brazilian Center of Research in Energy and Materials (CNPEM) Campinas Brazil

The control of gene expression by transcriptional regulators and other types of functional y relevant DNA transactions such as chromatin remodeling and replication underlie a vast spectrum of biological processes in al organisms. DNA transactions require the control ed interaction of proteins with DNA sequence motifs which are often located in nucleosome-depleted regions （NDRs） of the chromatin. Formaldehyde-assisted isolation of regulatory elements （FAIRE） has been established as an easy-to-implement method for the isolation of NDRs from a number of eukaryotic organisms, and it has been successful y employed for the discovery of new regulatory segments in genomic DNA from, for example, yeast, Drosophila, and humans. Until today, however, FAIRE has only rarely been employed in plant research and currently no detailed FAIRE protocol for plants has been published. Here, we provide a step-by-step FAIRE protocol for NDR discovery in Arabidopsis thaliana. We demonstrate that NDRs isolated from plant chromatin are readily amenable to quantitative polymerase chain reaction and next-generation sequencing. Only minor modification of the FAIRE protocol wil be needed to adapt it to other plants, thus facilitating the global inventory of regulatory regions across species.

关键词： Arabidopsis thaliana chromatin cis-regulatory elements epigenomics FAIRE-qPCR FAIRE-seq gene expression gene regulatory network transcription factor

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Neuroprotective agents effective against radiation damage of central nervous system

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Neural Regeneration Research 2022年第9期17卷 1885-1892页

作者： m.ria Lalkovičová Laboratory of Radiation Biology Joint Institute for Nuclear ResearchDubnaRussia Slovak Academy of Sciences Institute of Experimental PhysicsKošiceSlovakia

Ionizing radiation caused by medical treatments,nuclear events or even space flights can irreversibly damage structure and function of brain *** can result in serious brain damage,with memory and behavior disorders,or even fatal oncologic or neurodegenerative *** used treatments and drugs are mostly targeting biochemical processes of cell apoptosis,radiation toxicity,neuroinflammation,and conditions such as cognitive-behavioral disturbances or others that result from the radiation *** most drugs,the side effects and potential toxicity are also to be ***,many agents have not been approved for clinical use *** this review,we focus on the latest and most effective agents that have been used in animal and also in the human research,and clinical *** could have the potential therapeutical use in cases of radiation damage of central nervous system,and also in prevention considering their radioprotecting effect of nervous tissue.

关键词： antioxidants biomedical neuroprotectants central nervous system ionizing radiation neuroprotection radiation injury radiomitigators radioprotectants radioprotection therapeutics

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Is there a potential of circulating miRNAs as biomarkers in rheumatic diseases?

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Genes & Diseases 2023年第4期10卷 1263-1278页

作者： Klára Prajzlerová Ladislav Senolt m.ria Filková Institute of Rheumatology Prague 12800Czech Republic Department of Rheumatology First Faculty of MedicineCharles UniversityPrague 12800Czech Republic

t microRNAs(miRNAs)are small non-coding single-stranded RNAs of about 22 nucle-otides in length that act as post-transcriptional regulators of gene *** on the complementarity between miRNA and target mRNA,cleavage,destabilization,or transla-tional suppression of mRNA occurs within the RISC(RNA-induced silencing complex).As gene expression regulators,miRNAs are involved in a variety of biological *** of miRNAs and their target genes contribute to the pathophysiology of many diseases,including autoimmune and inflammatory *** are also present extracellularly in their sta-ble form in body *** incorporation into membrane vesicles or protein complexes with Ago2,HDL,or nucleophosmin 1 protects them against ***-free miRNAs can be deliv-ered to another cell in vitro and maintain their functional ***,miRNAs can be considered mediators of intercellular *** remarkable stability of cell-free miRNAs and their accessibility in body fluid makes them potential diagnostic or prognostic bio-markers and potential therapeutic *** we provide an overview of the potential role of circulating miRNAs as biomarkers of disease activity,therapeutic response,or diagnosis in rheumatic *** circulating miRNAs reflect their involvement in the pathogenesis,while for plenty,their pathogenetic mechanisms remain to be *** miRNAs described as biomarkers were also shown to be of therapeutic potential,and some miRNAs arealready tested in clinical trials.

关键词： Biomarker Diagnosis miRNA Rheumatic diseases Therapy

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Key elements determining the intestinal region-specific environment of enteric neurons in type 1 diabetes

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World Journal of Gastroenterology 2023年第18期29卷 2704-2716页

作者： m.ria Bagyánszki Nikolett Bódi Department of Physiology Anatomy and NeuroscienceFaculty of Science and InformaticsUniversity of SzegedSzeged H-6726Hungary

Diabetes,as a metabolic disorder,is accompanied with several gastrointestinal(GI)symptoms,like abdominal pain,gastroparesis,diarrhoea or *** and complex enteric nervous system damage is confirmed in the background of these diabetic motility *** anatomical length of the GI tract,as well as genetic,developmental,structural and functional differences between its segments contribute to the distinct,intestinal region-specific effects of *** observations support and highlight the importance of a regional approach in diabetes-related enteric *** large and microvessels are essential for the blood supply of enteric *** morpho-functional linkage exists between enteric neurons and enteroglia,however,there is also a reciprocal communication between enteric neurons and immune cells on which intestinal microbial composition has crucial *** this point of view,it is more appropriate to say that enteric neurons partake in multidirectional communication and interact with these key players of the intestinal *** interplays may differ from segment to segment,thus,the microenvironment of enteric neurons could be considered strictly *** goal of this review is to summarize the main tissue components and molecular factors,such as enteric glia cells,interstitial cells of Cajal,gut vasculature,intestinal epithelium,gut microbiota,immune cells,enteroendocrine cells,prooxidants,antioxidant molecules and extracellular matrix,which create and determine a gut region-dependent neuronal environment in diabetes.

关键词： Enteric neurons Neuronal environment Gut region specificity Type 1 diabetes,Hyperglycemia microbiota-gut interactions

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Epidemiology and Clinical Features of mycobacterium avium Complex Pulmonary Disease in a Brazilian Amazon Cohort

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Advances in microbiology 2024年第6期14卷 287-302页

作者： Jose Tadeu Colares monteiro Adriana Rodrigues Barretto Ruan Seguin Azevedo Quaresma Bianca miranda Gouveia Ismari Perini Furlaneto Victória menezes da Costa Yan Corrêa Rodrigues Layana Rufino Ribeiro Ricardo J. de Paula Souza e Guimarães Alex Brito Souza Ana Roberta Fusco da Costa Karla Valéria Batista Lima Joao de Barros Barreto University Hospital Federal University of Par Belm Brazil Center for Biological and Health Sciences Ph.D. Program in Parasitic Biology in the Amazon Region (PPGBPA) State University of Par (UEPA) Belm Brazil Department of medicine Centro Universitrio do Par CESUPA Belm Brazil Bacteriology and mycology Section Evandro Chagas Institute (SABMI/IEC) Ministry of Health Ananindeua Brazil Geoprocessing Laboratory Evandro Chagas Institute/SVSA/MS Ananineua Brazil

The incidence of mycobacterium avium complex pulmonary disease (mACPD) has been globally increasing. The present study aims to provide a comprehensive analysis of clinical characteristics and treatment response among patients in the Amazon region of Brazil and conduct a retrospective cohort study at a prominent referral hospital in the State of Par, Brazil, from 2012 to 2020. The m. avium group represented 58.9% of cases, followed by m. intracellulare (35.7%), m. colombiense (3.6%), and m. chimaera (1.8%). The majority of patients (73.2%) were female and with an average age of 63 years. Primary clinical manifestations included persistent cough and sputum production. The Charlson comorbidity index (CCI) averaged 2.47, with 36.3% of patients having a CCI score equal to higher than 3. Notably, over 70% of patients had received treatment for pulmonary tuberculosis before initiating treatment for mAC infection. The fibrocavitary radiologic form was the most prevalent (55.4%), frequently exhibiting a bilateral distribution (53.6%). Antimicrobial susceptibility revealed a significant prevalence of mAC resistance to drugs in the therapeutic regimen. Despite this, most of the patients experienced clinical improvement (50%). In conclusion, this study highlights a higher prevalence of mAC infections among middle-aged women, with a history of pulmonary tuberculosis treatment and presenting the fibrocavitary radiological form was predominant.

关键词： mACDP Nontuberculous mycobacteria mycobacterium avium Lung Disease

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male genitourinary schistosomiasis-related symptoms among long-term Western African migrants in Spain: a prospective population-based screening study

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贫困所致传染病(英文) 2024年第2期13卷 29-38页

作者： Roure Sílvia Vallès Xavier Pérez-Quílez Olga López-mu?oz Israel Chamorro Anna Abad Elena Valerio Lluís Soldevila Laura Espa?a Sergio Hegazy Alaa H. A. Fernández-Rivas Gema Gorriz Ester Herena Dolores Oliveira mário miralles m.ria Carme Conde Carmen montero-Alia Juan José Fernández-Pedregal Elia miranda-Sánchez Jose Llibre Josep m. Isnard mar Bonet Josep m.ria Estrada Oriol Prat Núria Clotet Bonaventura The Schisto-Stop study group International Health Program (PROSICS) Barcelona North Metropolitan Territorial Directorate for Infectious Diseases Catalan Institute for Health CAP La Salut Passatge dels Encants S/N Badalona Spain Fundació Lluita contra les Infeccions c/Can Ruti s/n Badalona Spain Infectious Diseases Department Hospital Universitari Germans Trias i Pujol c/Can Ruti s/n Badalona Spain Germans Trias i Pujol Research Institute c/Can Ruti s/n Badalona Spain microbiology Department Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Department of Genetics and microbiology Universitat Autònoma de Barcelona c/Can Ruti s/n Badalona Spain Faculty of medicine University of Asyut El Fateh Assiut Governorate Egypt Department of Urology Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Canovelles Primary Health Care Unit Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Indústria Canovelles Spain Primary Health Care Unit mataró-(Rocafonda-Palau) Barcelona North Metropolitan Health Directorate Catalan Institute for Health Camí Ral el Ravalet Mataró Barcelona Spain Barcelona North metropolitan Primary Care Directorate Catalan Institute for Health Ctra. de Barcelona Sabadell Barcelona Spain Directorate for Innovation and Interdisciplinary Cooperation Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain IrsiCaixa-AIDS Research Institute Hospital Universitari Germans Trias i Pujol University Hospital c/Can Ruti s/n Badalona Spain Infectious Diseases Directorate Barcelona North Metropolitan Health Directorate Catalan Institute for Health C/Can Ruti S/N Badalona Spain

Background Schistosomiasis is highly endemic in sub-Saharan Africa and frequently imported to Europe. male urogenital manifestations are often neglected. We aimed to ascertain the prevalence of genitourinary clinical signs and symptoms among long-term African migrants in a non-endemic European country using a serology *** We carried out a prospective, community-based cross-sectional study of adult male migrants from sub-Saharan Africa living in *** serology tests and microscopic urine examinations were carried out, and clinical data were obtained from an electronic medical record search and a structured *** We included 388 adult males, mean age 43.5 years [Standard Deviation (SD) = 12.0, range: 18–76]. The median time since migration to the European Union was 17 [Interquartile range (IQR): 11–21] years. The most frequent country of origin was Senegal (N = 179, 46.1%). Of the 338, 147 (37.6%) tested positive forSchistosoma. Parasite eggs were present in the urine of only 1.3%. Nine genitourinary clinical items were significantly associated with positiveSchistosoma serology results: pelvic pain (45.2%;OR = 1.57, 95%CI: 1.0–2.4), pain on ejaculation (14.5%;OR = 1.85, 95%CI: 1.0–3.5), dyspareunia (12.4%;OR = 2.45, 95%CI: 1.2–5.2), erectile dysfunction (9.5%;OR = 3.10, 95%CI: 1.3–7.6), self-reported episodes of infertility (32.1%;OR = 1.69, 95%CI: 1.0–2.8), haematuria (55.2%;OR = 2.37, 95%CI: 1.5–3.6), dysuria (52.1%;OR = 2.01, 95%CI: 1.3–3.1), undiagnosed syndromic STIs (5.4%), and orchitis (20.7%;OR = 1.81, 95%CI: 1.0–3.1). Clinical signs tended to *** Urogenital clinical signs and symptoms are prevalent among male African long-term migrants with a positiveSchistosoma serology results. Genital involvement can be frequent even among those with long periods of non-residence in their sub-Saharan African countries of origin. Further research is needed to develop diagnostic tools and validate therapeutic approaches to

关键词： Schistosomiasis Chronic schistosomiasis Urogenital schistosomiasis male genital schistosomiasis Longterm migrant

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Induced pluripotent stem cells for modeling and cell therapy of Parkinson＇s disease

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Neural Regeneration Research 2016年第5期11卷 727-728页

作者： m.ria Csobonyeiová Lubos Danisovic Stefan Polák Institute of Histology and Embryology Faculty of Medicine Comenius University in Bratislava Bratislava Slovakia Institute of medical Biology Genetics and Clinical Genetics Faculty of Medicine Comenius University in Bratislava Bratislava Slovakia

Neurodegenerative disorders include a variety of hereditary or sporadic diseases involving the chronic,progressive loss on neural ***＇s disease（PD）is the second most common neurodegenerative disease,affecting more than 6 million people worldwide（Wan et al.,2015）.Degeneration of nigrostriatal dopamine（DA）neurons is the main pathology in PD.

关键词： INDUCTION IPSCS

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Rilmenidine prevents blood pressure increase in rats with compromised nitric oxide production

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Acta Pharmacologica Sinica 2004年第12期25卷 74-80页

作者： m.ria GEROV,Jozef TRK,Ol’ga PECHV,Jana mATUKOV Institute ofNormal and Pathological Physiology SlovakAcademy of Sciences 813 71 Bratislava Slovak Republic Institute ofNormal and Pathological Physiology SlovakAcademy of Sciences 813 71 Bratislava Slovak Republic Institute ofNormal and Pathological Physiology SlovakAcademy of Sciences 813 71 Bratislava Slovak Republic Institute of Pathophysiology medical School Comenius University Bratislava Slovak Republic Now in Center for Blood Research and Department of Pathology Harvard medicalSchool Boston USA.

AIm: To search tools of high blood pressure in the model of nitric oxide (NO)-defective hypertension, and the study focused on the effect of rilmenidine, agonist of imidazoline receptors, which was suggested to modulate central sympathetic outflow. mETHODS: Three experimental groups, each consisting of 7 rats, were used: (I) rats with inhibition of NO synthase (NOS) by NG-nitro-L-arginine methyl ester (L-NAmE) 40 mg·kg-1·d-1 for 4 weeks in drinking water, (II) rats with inhibited NOS as in group I , plus agonist of imidazoline receptors rilmenidine 3 mg·kg-1·d-1 for 4weeks by gavage, and(III) control rats. Systolic blood pressure wasmeasured weekly noninvasively. At the end of experiment aortic ring isometric tension was followed, NOS expression (aorta, left ventricle), and NOS activity (left ventricle and brain) were determined. RESULTS: In the group I systolic blood pressure in- creased significantly, aortic ring relaxation to acetylcholine was significantly attenuated. Rilmenidine administered simultaneously with L-NAmE (group II) prevented the increase of blood pressure which did not differ significantly from control values; aortic ring relaxation to acetylcholine did not differ from control. No change in NOS expres- sion (aorta and left ventricle) was found in groups I and II. Significant decline in NOS activity (left ventricle and brain) wasfound in groups I and II. CONCLUSION: Rilmenidinehas aremarkable role inNO-defective hypertension, possibly by inhibiting central sympathetic outflow and by affecting receptors in vascular smooth muscle also. The prime cause of hypertension in this experimental model - the compromised production of NO due to inhibition of NOS - was not affected by rilmenidine.

关键词： NO deficiency hypertension imidazoline receptor nitric-oxide synthase sympathetic activity

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Receptor-like kinases and receptor-like proteins： keys to pathogen recognition and defense signaling in plant innate immunity

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Frontiers in Biology 2012年第2期7卷 155-166页

作者： Xin YANG Fengyang DENG katrina m. RAmONELL Department of Biological Sciences The University of Alabama Tuscaloosa AL 35487 USA College of Life Sciences Northwest A＆F University Yangling Shaanxi 712100 China

Plants have evolved multiple layers of defense against various pathogens in the environment. Receptor-like kinases/proteins （RLKs/RLPs） are on the front lines of the battle between plants and pathogens since they are present at the plasma membrane and perceive signature molecules from either the invading pathogen or damaged plant tissue. With a few notable exceptions, most RLKs/RLPs are positive regulators of plant innate immunity. In this review, we summarize recently discovered RLKs/RLPs that are involved in plant defense responses against various classes of pathogens, We also describe what is currently known about the mechanisms of RLK-mediated initiation of signaling via protein-protein interactions and phosphorylation.

关键词： receptor-like kinases (RLKs) receptor-like proteins (RLPs) biotrophic fungi necrotrophic fungi bacterial pathogens

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O_(3) based advanced oxidation for ibuprofen degradation

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Chinese Journal of Chemical Engineering 2022年第2期35卷 277-284页

作者： Vitória m.Almeida Carla A.Orge m.Fernando R.Pereira O.SaloméG.P.Soares Laboratory of Separation and Reaction Engineering-Laboratory of Catalysis and materials (LSRE-LCM)Faculdade de Engenharia da Universidade do PortoRua Dr.Roberto Frias4200-465 PortoPortugal

The degradation of the anti-inflammatory ibuprofen(IBP)was evaluated by several advanced oxidation *** was treated by single ozonation and oxidation with hydrogen peroxide(H_(2)O_(2)),as well as a combination of these *** order to improve the efficiency,the presence of catalysts such as original carbon nanotubes,labelled as CNT,and iron oxide supported on carbon nanotubes,named as Fe/CNT sample,was *** evolution of IBP degradation,mineralization and toxicity of the solutions was *** formation of intermediates was also *** the non-catalytic processes,IBP was faster removed by single ozonation,whereas no significant total organic carbon(TOC)removal was *** with H_(2)O_(2) did not present satisfactory *** ozone and H_(2)O_(2) were combined,a higher mineralization was attained(70%after 180 min of reaction).On the other hand,in the catalytic processes,this combined process allowed the fastest IBP *** terms of mineralization degree,the presence of Fe/CNT increases the removal rate in the first hour of reaction,achieving a TOC removal of 85%.Four compounds were detected as *** treated solutions presented lower toxicity than the initial solution,suggesting that the released intermediates during applied processes are less toxic.

关键词： Advanced oxidation processes Catalysts Emerging pollutants Ibuprofen

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