检索结果-南通市图书馆

A step-by-step protocol for formaldehyde-assi.ted i.olati.n of regulatory elements from Arabi.opsi. thali.na

Journal of i.tegrati.e Plant Bi.logy 2014年第6期56卷 527-538页

作者： mohammad Ami. Omi.bakhshfard Flavi. Vi.chi.Wi.ck Samuel Arvi.sson Di.go m.ria?o-Pachón Bernd mueller-Roeber Uni.ersi.y of Potsdam Institute of Biochemistry and Biology Potsdam-Golm 14476 Germany max-Planck i.sti.ute of molecular Plant Physi.logy Potsdam-Golm 14476 Germany Brazi.i.n Bi.ethanol Sci.nce and Technology Laboratory (CTBE) Brazilian Center of Research in Energy and Materials (CNPEM) Campinas Brazil

The control of gene expressi.n by transcri.ti.nal regulators and other types of functi.nal y relevant DNA transacti.ns such as chromati. remodeli.g and repli.ati.n underli. a vast spectrum of bi.logi.al processes i. al organi.ms. DNA transacti.ns requi.e the control ed i.teracti.n of protei.s wi.h DNA sequence moti.s whi.h are often located i. nucleosome-depleted regi.ns （NDRs） of the chromati.. Formaldehyde-assi.ted i.olati.n of regulatory elements （FAi.E） has been establi.hed as an easy-to-i.plement method for the i.olati.n of NDRs from a number of eukaryoti. organi.ms, and i. has been successful y employed for the di.covery of new regulatory segments i. genomi. DNA from, for example, yeast, Drosophi.a, and humans. Unti. today, however, FAi.E has only rarely been employed i. plant research and currently no detai.ed FAi.E protocol for plants has been publi.hed. Here, we provi.e a step-by-step FAi.E protocol for NDR di.covery i. Arabi.opsi. thali.na. We demonstrate that NDRs i.olated from plant chromati. are readi.y amenable to quanti.ati.e polymerase chai. reacti.n and next-generati.n sequenci.g. Only mi.or modi.i.ati.n of the FAi.E protocol wi. be needed to adapt i. to other plants, thus faci.i.ati.g the global i.ventory of regulatory regi.ns across speci.s.

关键词： Arabi.opsi. thali.na chromati. ci.-regulatory elements epi.enomi.s FAi.E-qPCR FAi.E-seq gene expressi.n gene regulatory network transcri.ti.n factor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neuroprotecti.e agents effecti.e agai.st radi.ti.n damage of central nervous system

引用

Neural Regenerati.n Research 2022年第9期17卷 1885-1892页

作者： m.ria Lalkovi.ová Laboratory of Radi.ti.n Bi.logy Joint Institute for Nuclear ResearchDubnaRussia Slovak Academy of Sci.nces Institute of Experimental PhysicsKošiceSlovakia

i.ni.i.g radi.ti.n caused by medi.al treatments,nuclear events or even space fli.hts can i.reversi.ly damage structure and functi.n of brai. *** can result i. seri.us brai. damage,wi.h memory and behavi.r di.orders,or even fatal oncologi. or neurodegenerati.e *** used treatments and drugs are mostly targeti.g bi.chemi.al processes of cell apoptosi.,radi.ti.n toxi.i.y,neuroi.flammati.n,and condi.i.ns such as cogni.i.e-behavi.ral di.turbances or others that result from the radi.ti.n *** most drugs,the si.e effects and potenti.l toxi.i.y are also to be ***,many agents have not been approved for cli.i.al use *** thi. revi.w,we focus on the latest and most effecti.e agents that have been used i. ani.al and also i. the human research,and cli.i.al *** could have the potenti.l therapeuti.al use i. cases of radi.ti.n damage of central nervous system,and also i. preventi.n consi.eri.g thei. radi.protecti.g effect of nervous ti.sue.

关键词： anti.xi.ants bi.medi.al neuroprotectants central nervous system i.ni.i.g radi.ti.n neuroprotecti.n radi.ti.n i.jury radi.mi.i.ators radi.protectants radi.protecti.n therapeuti.s

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Araucaria angusti.oli. Aboveground Roots Presented Hi.h Arbuscular mycorrhi.al Fungal Coloni.ati.n and Di.ersi.y i. the Brazi.i.n Atlanti. Forest

引用

Pedosphere 2016年第4期26卷 561-566页

作者： mi.ene mOREi.A m.ria i. zucchi Jose E. GOmES Si. m. TSAi.Alessandro ALVES-PEREi.A Elke J. B. N. CARDOSO Sao Paulo State Agency for Agri.usi.ess Technology CP 28 Piracicaba 13400-970 Center for Nuclear Energy i. Agri.ulture University of Sao Paulo Piracieaba 13416-000 Geneti.s Department Luiz de Queiroz College of Agriculture University of Sao Paulo Piraeieaba 13418-900 Soi. Sci.nce Department Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba 13418-900

30 di.ferent arbuscular mycorrhi.al fungi.（AmF） speci.s, di.tri.uted i. di.ferent genera such as Glomus, Acaulospora, Scutellospora, Entrophospora, Ambi.poru, Kuklospora, Gi.aspora, and Archeospora, have been i.enti.i.d i. the root zone of Araucaria angusti.oli., known as Brazi. Pi.e. Duri.g our AmF survey i. thi. ecosystem, our attenti.n was called to the presence of many superfi.i.lly growi.g Araucaria roots. Our hypothesi. was that these roots were coloni.ed wi.h AmF because of the presence of AmF spores i. organi. material aboveground. Samples of these superfi.i.l roots and the organi. substrate they were growi.g on were evaluated for thei. mycorrhi.al status. DNA was extracted from the AmF-coloni.ed superfi.i.l roots and submi.ted to polymerase chai. reacti.n （PCR） ampli.i.ati.n usi.g the NS31-Am1 pri.er pai., followed by cloni.g and sequenci.g. We found that the root coloni.ati.n percentages were between 31% and 52%, and the number of AmF spores i. the substrate ranged from 27 to 164 spores per 50 g dry substrate. The phylogeneti. analyses and tree constructi.n usi.g maxi.um parsi.ony （mP） and nei.hbor-joi.i.g （N J） methods i.enti.i.d 13 di.ferent speci.s of the phylum Glomeromycota belongi.g to the genera Glomus, Funneli.ormi., Rhi.ophagus, Gi.aspora, Acaulospora, and Archaeospora, and fi.e i.olates were i.enti.i.d only at the genus level. To our knowledge, thi. i. the fi.st report on Araucaria angusti.oli. wi.h roots growi.g aboveground, produci.g runner roots that develop on dead tree trunks and organi. material. The hi.her coloni.ati.n of the aboveground roots than those commonly found i. belowground Araucaria roots suggests that they may present acti.e metaboli. uptake of nutri.nts.

关键词： AmF spores fungal taxonomy mycorrhi.al status nutri.nt uptake organi. substrate root coloni.ati.n superfi.i.l roots

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

i. there a potenti.l of ci.culati.g mi.NAs as bi.markers i. rheumati. di.eases?

引用

Genes & Di.eases 2023年第4期10卷 1263-1278页

作者： Klára Prajzlerová Ladi.lav Senolt m.ria Fi.ková i.sti.ute of Rheumatology Prague 12800Czech Republic Department of Rheumatology First Faculty of MedicineCharles UniversityPrague 12800Czech Republic

t mi.roRNAs(mi.NAs)are small non-codi.g si.gle-stranded RNAs of about 22 nucle-oti.es i. length that act as post-transcri.ti.nal regulators of gene *** on the complementari.y between mi.NA and target mRNA,cleavage,destabi.i.ati.n,or transla-ti.nal suppressi.n of mRNA occurs wi.hi. the Ri.C(RNA-i.duced si.enci.g complex).As gene expressi.n regulators,mi.NAs are i.volved i. a vari.ty of bi.logi.al *** of mi.NAs and thei. target genes contri.ute to the pathophysi.logy of many di.eases,i.cludi.g autoi.mune and i.flammatory *** are also present extracellularly i. thei. sta-ble form i. body *** i.corporati.n i.to membrane vesi.les or protei. complexes wi.h Ago2,HDL,or nucleophosmi. 1 protects them agai.st ***-free mi.NAs can be deli.-ered to another cell i. vi.ro and mai.tai. thei. functi.nal ***,mi.NAs can be consi.ered medi.tors of i.tercellular *** remarkable stabi.i.y of cell-free mi.NAs and thei. accessi.i.i.y i. body flui. makes them potenti.l di.gnosti. or prognosti. bi.-markers and potenti.l therapeuti. *** we provi.e an overvi.w of the potenti.l role of ci.culati.g mi.NAs as bi.markers of di.ease acti.i.y,therapeuti. response,or di.gnosi. i. rheumati. *** ci.culati.g mi.NAs reflect thei. i.volvement i. the pathogenesi.,whi.e for plenty,thei. pathogeneti. mechani.ms remai. to be *** mi.NAs descri.ed as bi.markers were also shown to be of therapeuti. potenti.l,and some mi.NAs arealready tested i. cli.i.al trials.

关键词： Bi.marker Di.gnosi. mi.NA Rheumati. di.eases Therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Key elements determi.i.g the i.testi.al regi.n-speci.i. envi.onment of enteri. neurons i. type 1 di.betes

引用

World Journal of Gastroenterology 2023年第18期29卷 2704-2716页

作者： m.ria Bagyánszki.Ni.olett Bódi Department of Physi.logy Anatomy and NeuroscienceFaculty of Science and InformaticsUniversity of SzegedSzeged H-6726Hungary

Di.betes,as a metaboli. di.order,i. accompani.d wi.h several gastroi.testi.al(Gi.symptoms,li.e abdomi.al pai.,gastroparesi.,di.rrhoea or *** and complex enteri. nervous system damage i. confi.med i. the background of these di.beti. moti.i.y *** anatomi.al length of the Gi.tract,as well as geneti.,developmental,structural and functi.nal di.ferences between i.s segments contri.ute to the di.ti.ct,i.testi.al regi.n-speci.i. effects of *** observati.ns support and hi.hli.ht the i.portance of a regi.nal approach i. di.betes-related enteri. *** large and mi.rovessels are essenti.l for the blood supply of enteri. *** morpho-functi.nal li.kage exi.ts between enteri. neurons and enterogli.,however,there i. also a reci.rocal communi.ati.n between enteri. neurons and i.mune cells on whi.h i.testi.al mi.robi.l composi.i.n has cruci.l *** thi. poi.t of vi.w,i. i. more appropriate to say that enteri. neurons partake i. multi.i.ecti.nal communi.ati.n and i.teract wi.h these key players of the i.testi.al *** i.terplays may di.fer from segment to segment,thus,the mi.roenvi.onment of enteri. neurons could be consi.ered stri.tly *** goal of thi. revi.w i. to summari.e the mai. ti.sue components and molecular factors,such as enteri. gli. cells,i.tersti.i.l cells of Cajal,gut vasculature,i.testi.al epi.heli.m,gut mi.robi.ta,i.mune cells,enteroendocri.e cells,prooxi.ants,anti.xi.ant molecules and extracellular matri.,whi.h create and determi.e a gut regi.n-dependent neuronal envi.onment i. di.betes.

关键词： Enteri. neurons Neuronal envi.onment Gut regi.n speci.i.i.y Type 1 di.betes,Hyperglycemi. mi.robi.ta-gut i.teracti.ns

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ri.meni.i.e prevents blood pressure i.crease i. rats wi.h compromi.ed ni.ri. oxi.e producti.n

引用

Acta Pharmacologi.a Si.i.a 2004年第12期25卷 74-80页

作者： m.ria GEROV,Jozef TRK,Ol’ga PECHV,Jana mATUKOV i.sti.ute ofNormal and Pathologi.al Physi.logy SlovakAcademy of Sci.nces 813 71 Brati.lava Slovak Republi. i.sti.ute ofNormal and Pathologi.al Physi.logy SlovakAcademy of Sci.nces 813 71 Brati.lava Slovak Republi. i.sti.ute ofNormal and Pathologi.al Physi.logy SlovakAcademy of Sci.nces 813 71 Brati.lava Slovak Republi. i.sti.ute of Pathophysi.logy medi.al School Comeni.s Uni.ersi.y Brati.lava Slovak Republi. Now i. Center for Blood Research and Department of Pathology Harvard medi.alSchool Boston USA.

Ai.: To search tools of hi.h blood pressure i. the model of ni.ri. oxi.e (NO)-defecti.e hypertensi.n, and the study focused on the effect of ri.meni.i.e, agoni.t of i.i.azoli.e receptors, whi.h was suggested to modulate central sympatheti. outflow. mETHODS: Three experi.ental groups, each consi.ti.g of 7 rats, were used: (i. rats wi.h i.hi.i.i.n of NO synthase (NOS) by NG-ni.ro-L-argi.i.e methyl ester (L-NAmE) 40 mg·kg-1·d-1 for 4 weeks i. dri.ki.g water, (i.) rats wi.h i.hi.i.ed NOS as i. group i., plus agoni.t of i.i.azoli.e receptors ri.meni.i.e 3 mg·kg-1·d-1 for 4weeks by gavage, and(i.i. control rats. Systoli. blood pressure wasmeasured weekly noni.vasi.ely. At the end of experi.ent aorti. ri.g i.ometri. tensi.n was followed, NOS expressi.n (aorta, left ventri.le), and NOS acti.i.y (left ventri.le and brai.) were determi.ed. RESULTS: i. the group i.systoli. blood pressure i.- creased si.ni.i.antly, aorti. ri.g relaxati.n to acetylcholi.e was si.ni.i.antly attenuated. Ri.meni.i.e admi.i.tered si.ultaneously wi.h L-NAmE (group i.) prevented the i.crease of blood pressure whi.h di. not di.fer si.ni.i.antly from control values; aorti. ri.g relaxati.n to acetylcholi.e di. not di.fer from control. No change i. NOS expres- si.n (aorta and left ventri.le) was found i. groups i.and i.. Si.ni.i.ant decli.e i. NOS acti.i.y (left ventri.le and brai.) wasfound i. groups i.and i.. CONCLUSi.N: Ri.meni.i.ehas aremarkable role i.NO-defecti.e hypertensi.n, possi.ly by i.hi.i.i.g central sympatheti. outflow and by affecti.g receptors i. vascular smooth muscle also. The pri.e cause of hypertensi.n i. thi. experi.ental model - the compromi.ed producti.n of NO due to i.hi.i.i.n of NOS - was not affected by ri.meni.i.e.

关键词： NO defi.i.ncy hypertensi.n i.i.azoli.e receptor ni.ri.-oxi.e synthase sympatheti. acti.i.y

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Regi.n-dependent effects of di.betes and i.suli.-replacement on neuronal ni.ri. oxi.e synthase-and heme oxygenase-i.munoreacti.e submucous neurons

引用

World Journal of Gastroenterology 2017年第41期23卷 7359-7368页

作者： Ni.olett Bódi.Zi.a Szalai.Lali.ha Chandrakumar m.ria Bagyánszki Department of Physi.logy Anatomy and Neuro-science Faculty of Science and Informatics University of Szeged

Ai. To i.vesti.ate the i.testi.al segment-speci.i. effects of di.betes and i.suli. replacement on the densi.y of di.ferent subpopulati.ns of submucous neurons. mETHODS Ten weeks after the onset of type 1 di.betes samples were taken from the duodenum, i.eum and colon of streptozotoci.-i.duce di.beti., i.suli.-treated di.beti. and sex-and age-matched control rats. Whole-mount preparati.ns of submucous plexus were prepared from the di.ferent gut segments for quanti.ati.e fluorescent i.munohi.tochemi.try. The followi.g double-i.munostai.i.gs were performed: neuronal ni.ri. oxi.e synthase(n NOS) and Hu C/D, heme oxygenase(HO) 1 and peri.heri., as well as HO2 and peri.heri.. The densi.y of n NOS-, HO1-and HO2-i.munoreacti.e(i.) neurons was determi.ed as a percentage of the total number of submucous neurons. RESULTS The total number of submucous neurons and the proporti.n of n NOS-, HO1-and HO2-i. subpopulati.ns were not affected i. the duodenal gangli. of control, di.beti. and i.suli.-treated rats. Whi.e the total neuronal number di. not change i. ei.her the i.eum or the colon, the densi.y of ni.rergi. neurons exhi.i.ed a 2-and 3-fold i.crease i. the di.beti. i.eum and colon, respecti.ely, whi.h was further enhanced after i.suli. replacement. The presence of HO1-and HO2-i. submucous neurons was robust i. the colon of controls(38.4%-50.8%), whereas i. was si.ni.i.antly lower i. the small i.testi.al segments(0.0%-4.2%, P i.logi.al condi.i.ns the only alterati.n detected was an i.crease i. the i.eum and a decrease i. the colon of the proporti.n of HO-i. neurons i. i.suli.-treated di.beti. ani.als. CONCLUSi.N Di.betes and i.medi.te i.suli. replacement i.duce the most pronounced regi.n-speci.i. alterati.ns of n NOS-, HO1-and HO2-i. submucous neuronal densi.y i. the di.tal parts of the gut.

关键词： Ni.rergi. neurons Heme oxygenase 1 Heme oxygenase 2 Submucous neurons Gut regi.nspeci.i.i.y Di.betes i.suli.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tensi.e and fracture properti.s of an mg-RE-Zn alloy at elevated temperatures

引用

Journal of Rare Earths 2014年第6期32卷 564-572页

作者： TROJANOVZuzanka DONi. Ti.or LUK Pavel PALEK Peter CHALUPOV m.ria Ti.LOV Eva BASTOVANSKY Ronald Faculty of mathemati.s and Physi.s Charles University Faculty of mechani.al Engi.eeri.g University of Zilina

magnesi.m alloy EZ10 （mg-RE-Zn） was deformed i. tensi.n at temperatures from 20 up to 520℃. A rapi. decrease of the yi.ld and tensi.e strength wi.h temperature was observed at temperatures hi.her than 300 ℃. On the other hand, ducti.i.y of sam-ples rapi.ly i.creased i. the same temperature range. Li.ht mi.roscopy and scanni.g electron mi.roscopy was used to reveal the rea-son for these behavi.urs. i.termetalli. parti.les i. grai. boundari.s are responsi.le for excellent mechani.al properti.s at lower tem-peratures. Di.fusi.nal processes occurri.g at temperatures hi.her than 300 ℃ si.ni.i.antly i.fluenced the deformati.n mechani.m as well as the fracture character.

关键词： magnesi.m alloy tensi.n di.locati.n sli. twi.ni.g dynami. recrystalli.ati.n grai. boundary sli.i.g, fracture, cavi.ati.n rare

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluati.n of granulabi.i.y and absorbti.i.y of bi.mass i. agglomerati.n mi.ture

引用

Journal of Central South Uni.ersi.y 2017年第10期24卷 2260-2265页

作者： Hudák Jozef Lesko Jaroslav Semanová Zuzana Frohli.hová m.ria Fi.dorák Róbert Legemza Jaroslav i.sti.ute of metallurgy Faculty of MetallurgyTechnical University of KosiceKosice04200Slovakia

The granulabi.i.y and absorbti.i.y of sawdust and coke breeze were *** properti.s are i.portant wi.hi. the preparati.n of agglomerati.n *** have a si.ni.i.ant i.pact on plynodynami. batch *** of materials to wet i. a fundamental condi.i.n of granulabi.i.y of *** and water absorpti.n of wood wi.h li.ui.s i. generally si.ni.i.antly affected by thei. chemi.al *** i.portant factors affecti.g the wetti.g process are undoubtedly the wood moi.ture content,the value of the surface tensi.n of li.ui. medi. and thei. chemi.al *** i.creasi.g moi.ture content of wood,the ti.e to equi.i.ri.m *** of water absorpti.n and granulabi.i.y of sawdust was conducted to seek thei. possi.le use as a parti.l replacement of coke dust i. the producti.n of the *** have been reali.ed wi.h alder,oak and pi.e sawdust and reference material-coke *** materials were di.i.ed to four classes of granulari.y:suppli.d material,1-2 mm,0.5-1 mm and less than 0.5 *** di.ferent li.ui. medi. were used:water 16°C,water 60°C,soapy water,li.e mi.k and surfactant.

关键词： agglomerati.n bi.mass coke breeze granulabi.i.y absorbti.i.y

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

O_(3) based advanced oxi.ati.n for i.uprofen degradati.n

引用

Chi.ese Journal of Chemi.al Engi.eeri.g 2022年第2期35卷 277-284页

作者： Vi.ória m.Almei.a Carla A.Orge m.Fernando R.Perei.a O.SaloméG.P.Soares Laboratory of Separati.n and Reacti.n Engi.eeri.g-Laboratory of Catalysi. and materials (LSRE-LCM)Faculdade de Engenharia da Universidade do PortoRua Dr.Roberto Frias4200-465 PortoPortugal

The degradati.n of the anti.i.flammatory i.uprofen(i.P)was evaluated by several advanced oxi.ati.n *** was treated by si.gle ozonati.n and oxi.ati.n wi.h hydrogen peroxi.e(H_(2)O_(2)),as well as a combi.ati.n of these *** order to i.prove the effi.i.ncy,the presence of catalysts such as ori.i.al carbon nanotubes,labelled as CNT,and i.on oxi.e supported on carbon nanotubes,named as Fe/CNT sample,was *** evoluti.n of i.P degradati.n,mi.erali.ati.n and toxi.i.y of the soluti.ns was *** formati.n of i.termedi.tes was also *** the non-catalyti. processes,i.P was faster removed by si.gle ozonati.n,whereas no si.ni.i.ant total organi. carbon(TOC)removal was *** wi.h H_(2)O_(2) di. not present sati.factory *** ozone and H_(2)O_(2) were combi.ed,a hi.her mi.erali.ati.n was attai.ed(70%after 180 mi. of reacti.n).On the other hand,i. the catalyti. processes,thi. combi.ed process allowed the fastest i.P *** terms of mi.erali.ati.n degree,the presence of Fe/CNT i.creases the removal rate i. the fi.st hour of reacti.n,achi.vi.g a TOC removal of 85%.Four compounds were detected as *** treated soluti.ns presented lower toxi.i.y than the i.i.i.l soluti.n,suggesti.g that the released i.termedi.tes duri.g appli.d processes are less toxi..

关键词： Advanced oxi.ati.n processes Catalysts Emergi.g pollutants i.uprofen

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：