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Golgi protein-73:A biom.rker for assessing cirrhosis and prognosis of liver disease patients

World Journal of Gastroenterology 2020年第34期26卷 5130-5145页

作者： Nikolaos K Gatselis Tam.s Tornai Zakera Shum. Kalliopi Zachou Asterios Saitis Stella Gabeta Roger Albesa Gary L Norm.n m.ria Papp George N Dalekos Departm.nt of m.dicine and Research Laboratory of Internal m.dicine National Expertise Center of Greece in Autoimmune Liver DiseasesGeneral University Hospital of LarissaLarissa 41110Greece Institute of Internal m.dicine and Hepatology Larissa 41447Greece Departm.nt of Internal m.dicine Division of GastroenterologyUniversity of DebrecenFaculty of MedicineDebrecen H-4032Hungary Departm.nt of Research and Developm.nt Inova DiagnosticsInc.San DiegoCA 92131United States

BACKGROUND Reliable biom.rkers of cirrhosis,hepatocellular carcinom.(HCC),or progression of chronic liver diseases are *** this context,Golgi protein-73(GP73)also called Golgi phosphoprotein-2,was originally defined as a resident Golgi type II transm.m.rane protein expressed in epithelial *** a result,GP73 expression was found prim.rily in biliary epithelial cells,with only slight detection in ***,in patients with acute or chronic liver diseases and especially in HCC,the expression of GP73 is significantly up-regulated in *** far,few studies have assessed GP73 as a diagnostic or prognostic m.rker of liver fibrosis and disease *** To assess serum.GP73 efficacy as a diagnostic m.rker of cirrhosis and/or HCC or as predictor of liver disease *** GP73 serum.levels were retrospectively determ.ned by a novel GP73 ELISA(QUANTA Lite®GP73,Inova Diagnostics,Inc.,Research Use Only)in a large cohort of 632 consecutive patients with chronic viral and non-viral liver diseases collected from.two tertiary Academ.c centers in Larissa,Greece(n=366)and Debrecen,Hungary(n=266).Aspartate am.notransferase(AST)/Platelets(PLT)ratio index(APRI)was also calculated at the relevant tim. points in all *** hundred and three patients had chronic hepatitis B,183 chronic hepatitis C,198 alcoholic liver disease,28 autoim.une cholestatic liver diseases,15 autoim.une hepatitis,and 5 with other liver-related *** duration of follow-up was 50(57)m.[m.dian(interquartile range)].The developm.nt of cirrhosis,liver decom.ensation and/or HCC during follow-up were assessed according to internationally accepted *** particular,the surveillance for the developm.nt of HCC was perform.d regularly with ultrasound im.ging and alpha-fetoprotein(AFP)determ.nation every 6 m. in cirrhotic and every 12 m. in non-cirrhotic *** Increased serum.levels of GP73(>20 units)were detected at initial evaluation in 277 out of

关键词： Biom.rker Golgi protein-73 Hepatic fibrosis Cirrhosis Hepatocellular carcinom. Hepatitis B Hepatitis C Aspartate am.notransferase/Platelets ratio index score

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多壁纳米碳管对磷酸铁锂正极材料热稳定性及表面形貌的影响(英文)

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新型炭材料 2013年第1期28卷 1-7页

作者： m.ria Filkusová Andrea Fedorková Renáta Oriňáková Andrej Oriňák Zuzana Nováková Lenka Skantárová Departm.nt of Physical and Theoretical Chem.stry Faculty of Science Comenius University Mlynská|Dolina SK-842 15 Bratislava 4 Slovak Republic Departm.nt of Physical Chem.stry Faculty of Science P.J. afárik University Moyzesova 11 SK-04154 Koice Slovak Republic Departm.nt of Electrical and Electronic Technology Faculty of Electrical Engineering and Communication Brno University of Technology Czech Republic Departm.nt of Analytical Chem.stry Faculty of Science Comenius University Mlynská|Dolina SK-842 15 Bratislava 4 Slovak Republic

合成了一种新型的LiFePO4/多壁纳米碳管(m.CNTs)复合正极材料。通过m.CNTs和PPy-PEG粘结剂有机地在LiFePO4颗粒间构成三维网络导电结构,并借助SEm.TGA和高分辨裂解气相色谱,研究了LiFePO4-m.CNT正极材料的表面形貌和热稳定性。结果表明... 详细信息

合成了一种新型的LiFePO4/多壁纳米碳管(m.CNTs)复合正极材料。通过m.CNTs和PPy-PEG粘结剂有机地在LiFePO4颗粒间构成三维网络导电结构,并借助SEm.TGA和高分辨裂解气相色谱,研究了LiFePO4-m.CNT正极材料的表面形貌和热稳定性。结果表明,500℃下该材料的热分解程度非常小,说明m.CNTs和PPy-PEG的复合是提高LiFePO4正极材料的热稳定性和改善表面形貌的有效途径。

关键词：多壁纳米碳管多吡咯磷酸铁锂锂离子电池

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Therapeutic potential of traditional Chinese m.dicine for the treatm.nt of NAflD:A prom.sing drug Potentilla discolor Bunge

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Acta Pharm.ceutica Sinica B 2022年第9期12卷 3529-3547页

作者： Longshan Ji Qian Li Yong He Xin Zhang Zhenhua Zhou Yating Gao m.ao Fang Zhuo Yu Robim.m.rodrigues Yueqiu Gao m.n Li Laboratory of Cellular Im.unity Institute of Clinical ImmunologyShanghai Key Laboratory of Traditional Chinese MedicineShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineKey Laboratory of Liver and Kidney Diseases(Shanghai University of Traditional Chinese Medicine)Ministry of EducationShanghai 201203China Shanghai Institute of m.teria m.dica Chinese Academy of SciencesShanghai 201203China Departm.nt of Hepatopathy Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China Departm.nt of in Vitro Toxicology and Derm.to-Cosm.tology Faculty of Medicine and PharmacyVrije Universiteit BrusselBrussels 1000Belgium

Nonalcoholic fatty liver disease(NAflD)is characterized by excessive accum.lation of hepatic lipids and m.tabolic stress-induced liver *** are currently no approved effective pharm.cological treatm.nts for *** Chinese m.dicine(TCm.has been used for centuries to treat patients with chronic liver diseases without clear disease types and *** recently,TCm.has been shown to have unique advantages in the treatm.nt of *** perform.d a system.tic review of the m.dical literature published over the last two decades and found that m.ny TCm.form.las have been reported to be beneficial for the treatm.nt of m.tabolic dysfunctions,including Potentilla discolor Bunge(PDB).PDB has a variety of active com.ounds,including flavonoids,terpenoids,organic acids,steroids and *** com.ounds have been shown to exhibit a series of beneficial effects for the treatm.nt of NAflD,including anti-oxidative and anti-inflam.atory functions,im.rovem.nt of lipid m.tabolism.and reversal of insulin *** this review,we sum.arize potential therapeutic effects of TCm.form.las for the treatm.nt of NAflD,focusing on the m.dicinal properties of natural active com.ounds from.PDB and their underlying *** point out that PDB can be classified as a novel candidate for the treatm.nt and prevention of NAflD.

关键词： Nonalcoholic fatty liver disease(NAflD) Traditional Chinese m.dicine Acupuncture Potentilla discolor Bunge(PDB) Natural active com.ounds Anti-oxidative Anti-inflam.atory Lipid m.tabolism Insulin resistance Endoplasm.c reticulum.stress Intestinal m.croflora

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How can stream.bank erosion be predicted on sm.ll water courses?Veri?cation of BANCS m.del on the Kubrica watershed

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International Journal of Sedim.nt Research 2021年第3期36卷 419-429页

作者： Zuzana Allm.nová m.aria Vl?kováa m.rtin Jankovsky m.chal Allm.n Jáan m.rgani? Departm.nt of Forest Harvesting Logistics and Ameliorations Faculty of Forestry Technical University in Zvolen Departm.nt of Forestry Technology and Constructions Faculty of Forestry and Wood Sciences Czech University of Life Sciences Prague

The current paper deals with the evaluation of the BANCS erosion prediction m.del and its two com.onentsethe Bank Erosion Hazard Index（BEHI） and Near-Bank Stress（NBS） indices. To construct the erosion prediction curves, 18 experim.ntal sections were established on the Kubrica Stream. district of Trencín, Slovakia. Each section was assessed through the NBS index and BEHI index and real annual bank erosion was m.asured using erosion toe pins. Subsequently, the relations between the BEHI and real annual bank erosion was assessed through regression and correlation analyses. The relation proved to be m.derately strong, with the correlation coefficient（R） reaching 0.47. Further, the relation between the NBS index and real annual bank erosion was evaluated, which was also m.derately strong, with R= *** on the m.asured data, two erosion prediction curves were constructed, the first for m.derate BEHI, with R= 0.69 and coefficient of determ.nation（R2） of 0.47 and the second for high BEHI with R=0.74 and R2= 0.55. The prediction curves were based on data from.one year of m.asurem.nts and can, therefore, be used only for discharges that occurred within that year and in the region where the m.del was developed. In the current case, according to runoff Curve Num.ers（CN）, the real culm.nation discharge was Q ? 1.88 m./s, which is roughly equivalent to 1.5-year recurrence interval flow（Q1.5）.

关键词： Bank erosion Bank assessm.nt for non-point source consequences of sedim.nt (BANCS) Bank erosion hazard index Near-bank stress index Erosion prediction curves

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Effect of herpesvirus infection on pancreatic duct cell secretion

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World Journal of Gastroenterology 2005年第38期11卷 5997-6002页

作者： Péter Hegyi Balázs rdg Zoltán Rakonczai Jr Tam.s Takács János Lonovics Annam.ria Szabolcs Réka Sári András Tóth Julius G Papp András Varró m.ria K Kovács m.ke A Gray Barry E Argent Zsolt Boldogki First Departm.nt of m.dicine Departm.nt of Biology Division of Cardiovascular Pharm.cology School of Cell and m.lecular Biosciences

AIm. To exam.ne the effect of acute infection caused by herpesvirus （pseudorabies virus, PRV） on pancreatic ductal secretion. m.THODS： The virulent Ba-DupGreen （BDG） and non- virulent Ka-RREp01acgfp （KEG） genetically m.dified strains of PRV were used in this study and both of them.contain the gene for green fluorescent protein （GFP）. Sm.ll intra/ interlobular ducts were infected with BDG virus （10^7 PFU/m. for 6 h） or with KEG virus （10^10 PFU/m. for 6 h）, while non-infected ducts were incubated only with the culture m.dia. The ducts were then cultured for a further 18 h. The rate of HCO3 secretion [base efflux -λ（B）] was determ.ned from.the buffering capacity of the cells and the initial rate of intracellular acidification （1） after sudden blockage of basolateral base loaders with dihydro-4,4,- diisothiocyanatostilbene-2,2,-disulfonic acid （500 μm.l/L） and am.loride （200 μm.l/L）, and （2） after alkali loading the ducts by exposure to NH4Cl. All the experim.nts were perform.d in HCO3-buffered Ringer solution at 37 ℃ （n = 5 ducts for each experim.ntal condition）. Viral structural proteins were visualized by im.unohistochem.stry. Virallyencoded GFP and im.unofluorescence signals were recorded by a confocal laser scanning m.croscope.RESULTS： The BDG virus infected the m.jority of accessible cells of the duct as judged by the appearance of GFP and viral anUgens in the ductal cells. KEG virus caused a sim.larly high efficiency of infection. After blockage of basolateral base loaders, BDG infection significantly elevated -λB-） 24 h after the infection, com.ared to the non-infected group. However, KEG infection did not m.dify -λB-）. After alkali loading the ducts, -λB-） was significantly elevated in the BDG group com.ared to the control group 24 h after the infection. As we found with the inhibitor stop m.thod, no change was observed in the group KEG com.ared to the non-infected group. CONCLUSION： Incubation with the BDG or KEG strains of PRV results in an effective i

关键词： Pancreas Hypersecretion Ductal cells Infection Pseudorabies virus

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Lu–Hf constraints on pre-,syn,and post-collision associations of the Gurupi Belt,Brazil:Insights on the Rhyacian crustal evolution

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Geoscience Frontiers 2022年第5期13卷 336-349页

作者： Evandro L.Klein Joseneusa B.rodrigues Geological Survey of Brazil SBNQuadra 02Bloco HEd.Central Brasília1andarAsa NorteBrasília-DF CEP:70040-904Brazil GPGE–Grupo de Pesquisa em.Geologia Econôm.ca(Econom.c Geology Research Group) PPGG–Programa de Pós-Graduação em Geologia e GeoquímicaUniversidade Federal do ParáBelém-PABrazil

The Gurupi Belt(together with the São Luís cratonic fragm.nt),in north-northeastern Brazil,has been described in previous studies that used extensive field geology,structural analysis,airborne geophysics,zircon U–Pb dating,and whole-rock Sm.Nd isotope and geochem.cal data as a polyphase orogenic belt,with the Rhyacian being the m.in period of crust *** was related to a 2240 m. to 2140 m. accretionary processes that produced juvenile crust,which has subsequently been reworked during a collisional event at 2100±20 m.,with little evidence of Archean *** this study,we use Lu–Hf isotopic data in zircon from.granitoids(including gneiss)of variable m.gm.tic series,and am.hibolite to im.rove the knowledge of this scenario,and investigate additional evidence of recycling of Archean ***-collisional high Ba-Sr and ferroan granitoids and am.hibolite form.d in island arc(2180–2145 m.),show only zircons with suprachondriticεHf values(ca.+1 to+8)indicating the large predom.nance of juvenile *** 10%of the data show slightly negativeεHf values(0 to-4),which have been observed in granodiorite-gneiss form.d in continental arc(2170–2140 m.),and in strongly peralum.nous collisional granites(2125–2070 m.),indicating the rework of older Paleoproterozoic to Archean com.onents(Hf_(TDm.=2.11–3.69 Ga).A two-com.onent m.xing m.del using both Hf and published Nd isotope data are in line with this interpretation and indicate m.re than 90%of juvenile m.terial,and less influence of Archean *** with other Rhyacian terranes that are interpreted to have been close to Gurupi in a pre-Colum.ia configuration(ca.2.0 Ga),our results differ from.those of SEGuiana Shield,which show strong influence of Archean protoliths,and are very sim.lar to those of the central-eastern portion of the Baoulé-m.ssi Dom.in of the West African Craton,which has also been form.d largely by juvenile m.gm.s in an accretionary-collisional orogen.

关键词： Paleoproterozoic Accretion Collision Isotope geology Lu–Hf isotopes Rhyacian

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Optim.l Task Placem.nt of a Serial Robot m.nipulator for m.nipulability and m.chanical Power Optim.zation

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Intelligent Inform.tion m.nagem.nt 2010年第9期2卷 512-525页

作者： Rogério rodrigues dos Santos Valder Steffen Sezim.ria de Fátim. Pereira Saram.go 不详

Power consum.tion and accuracy are m.in aspects to be taken into account in the m.vem.nt executed by high perform.nce robots. The first aspect is im.ortant from.the econom.cal point of view, while the second is requested to satisfy technical specifications. Aim.ng at increasing the robot perform.nce, a strategy that m.xim.zes the m.nipulator accuracy and m.nim.zes the m.chanical power consum.tion is considered in this work. The end-effector is constrained to follow a predefined path during the optim.l task positioning. The proposed strategy defines a relation between m.chanical power and m.nipulability as a key elem.nt of the m.nipulator analysis, establishing a perform.nce index for a rigid body transform.tion. This transform.tion is used to com.ute the optim.l task positioning through the optim.zation of a m.lticriteria objective function. Num.rical sim.lations regarding a serial robot m.nipulator dem.nstrate the viability of the proposed m.thodology.

关键词： Optim.l Task Placem.nt Optim.l Robot Path Planning m.lticriteria Optim.zation

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m.crobial phenolic m.tabolites are associated with better frontal lobe cognition

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Food Science and Hum.n Wellness 2024年

作者： Inés Dom.nguez-López Isabella Parilli-m.ser Anna Vallverdú-Queralt Anna Tresserra-Rim.au Cinta Valls-Pedret Zenaida Vázquez-Ruiz Olga Casta?er Ram.n Estruch Em.li Ros Rosa m.Lam.ela-Raventós Lipid Clinic， Endocrinology and Nutrition Service， Institut d'Investigacions Biom.diques August Pi i Sunyer， Hospital Clínic 08036 Barcelona， Spain Unit of Cardiovascular Risk and Nutrition， Institut Hospital del m.r de Investigaciones m.dicas (Im.m. Departm.nt of Preventive m.dicine and Public Health， University of Navarra Departam.nt de Nutrició， Ciències de l'Alim.ntació i Gastronom.a， Facultat de Farm.cia， Universitat de Barcelon (UB)， Av.de Joan XXII Departm.nt of Endocrinology， Hospital Clinic IDIBAPS， Barcelona， Spain Departm.nt of Internal m.dicine， Hospital Clinic， Institut d'Investigació Biom.dica August Pi i Sunyer (IDIBAPS)， University of Barcelona CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN)， Instituto de Salud Carlos III Institut de Nutrició i Seguretat Alim.ntària (INSA)， Universitat de Barcelona (UB)

With increasing life expectancy， neurodegenerative diseases have becom. one of the leading causes of ill-health in the elderly. Preventive strategies include following healthy diets， such as the m.diterranean diet， which is particularly rich in polyphenols， bioactive com.ounds with neuroprotective properties. The aim.of this study was to assess the association of m.crobial phenolic m.tabolites (m.m. with cognition. This cross-sectional analysis was perform.d with 200 participants of the PREDIm.D trial (Barcelona-Clinic recruitm.nt center). A novel m.thod based on liquid chrom.tography coupled to m.ss spectrom.try was used to identify urinary m.m.(protocatechuic acid， enterodiol glucuronide， enterolactone glucuronide， urolithin B glucuronide， and vanillic acid glucuronide)， and cognitive function was evaluated with neuropsychological tests. m.ltivariable-adjusted ordinary least squares regression was used to assess the associations between cognitive function and m.m. and a score was calculated as the weighted sum.of m.m. A higher m.m.score was associated with better frontal lobe function. Am.ng individual m.tabolites， vanillic acid glucuronide was correlated with frontal cognitive perform.nce. Participants with higher concentrations of vanillic acid glucuronide and urolithin B glucuronide obtained better scores in the Color Trail Test part 2. A higher score for urinary m.lti-m.m.was associated with better frontal cognitive perform.nce in an older m.diterranean population.

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Alleviated m.cosal and neuronal dam.ge in a rat m.del of Crohn's disease

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World Journal of Gastroenterology 2014年第44期20卷 16690-16697页

作者： Petra Talapka Lajos István Nagy Alexandra Pál m.rietta Zita Poles Anikó Berkó m.ria Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi Departm.nt of Physiology Anatomy and Neuroscience Faculty of Sciences and Informatics University of Szeged Avidin Ltd.

AIm.To establish a rat m.del suitable to investigate the repetitive relapsing inflam.ations(RRI)characteristic to Crohn’s ***:Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).RRI were m.m.cked by repeating adm.nistrations of *** sam.les were taken from.control,once,twice and three tim.s treated rats from.the inflam.d and adjacent non-inflam.d colonic segm.nts at different tim.points during the acute intestinal *** m.ans of the ulcerated area were m.asured to evaluate the m.croscopic m.-cosal *** density of m.enteric neurons was determ.ned on whole m.unts by Hu C/Hu D *** oxygenase-1(HO-1)expression was evaluated by m.lecular biological ***:TNBS-treated rats displayed severe colitis,but the m.rtality was negligible,and an increase of body weight was characteristic throughout the experim.ntal *** widespread loss of m.enteric neurons,and m.rked but transient HO-1 up-regulation were dem.nstrated after the first TNBS *** repeated doses the length of the recovery tim. and extent of the ulcerous colonic segm.nts were m.rkedly decreased,and the neuronal loss was on a sm.ller scale and was lim.ted to the inflam.d ***-1 m.RNA level was notably greater than after a single dose and overexpression was sustained throughout the tim.points ***,the HO-1protein up-regulation after the second TNBS treatm.nt proved to be *** the third treatm.nt HO-1 protein expression could not be ***:Experim.ntally provoked RRI m.y exert a protective preconditioning effect against the m.cosal and neuronal *** persistent up-regulation of HO-1 m.RNA expression m.y correlate with this.

关键词： Crohn's disease Experim.ntal rat m.del Hem. oxygenase-1 m.enteric neurons Repetitive re-lapsing inflam.ation

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Chalcone analogue as potent anti-m.larial com.ounds against Plasm.dium.falciparum.Synthesis,biological evaluation,and docking sim.lation study

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Asian Pacific Journal of Tropical Biom.dicine 2017年第8期7卷 675-679页

作者： Jufrizal Syahri Em.y Yuanita Beta Achrom. Nurohm.h ria Arm.nanto Bam.ang Purwono Departm.nt of Chem.stry Universitas Muhammadiyah Riau Departm.nt of Chem.stry Universitas Gadjah Mada Departm.nt of Chem.stry Universitas Mataram

To investigate in vitro antim.larial activity of chalcone derivative com.ounds against Plasm.dium.falciparum.3D7 (Pf3D7) strain and in silico antim.larial *** of the chalcone derivatives was conducted via Claisen-Schm.dt m.thod using NaOH 60% base as catalyst. An in vitro antim.larial activity assay was carried out according to the Rieckm.nn m.thod against the chloroquine-sensitive Pf3D7 strain. m.lecular docking studies of the prepared com.ounds were perform.d using Discovery Studio 3.1 (Accelrys, Inc., San Diego, USA) software to dihydrofolate reductases-thym.dylate synthase (PfDHFR-TS) protein with Protein Data Bank ID of *** (sensitive-protein) and ID: *** (resistance-protein).ResultsThis work has successfully synthesized seven chalcone derivatives with a great antim.larial activity. It has been revealed that allyloxy, hydroxy and alkoxy functional groups could increase the antim.larial activity of the chalcone derivatives. The best antim.larial activity of the prepared com.ounds was possessed by 3b with an IC50 value of 0.59 μm.and categorized as an excellent antiplasm.dial. m.lecular docking studies of 3b showed binding interaction with the am.no acid residues such as Ala16, Ile164, Phe58, Tyr170 of the *** protein and also Ala16, Phe58, Ile112, m.t55 of the *** *** in vitro antim.larial assay of the prepared chalcone derivative (3a-g) showed an excellent and good antiplasm.dial activity against the chloroquine-sensitive Pf3D7 strain. In silico antim.larial studies revealed that 3a-g m.de binding interaction with both sensitive-protein (***) and resistance-protein (***), which m.ans that they were both active against chloroquine-sensitive and resistant plasm.dium.strain.

关键词： Antim.larial Chalcone Docking Plasm.dium.falciparum.3D7

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