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In vitro and in vivo evaluation of the antiangiogenic activities of Trigonella foenum-graecum extracts

Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 732-738页

作者： Zina A.Habib-martin Hana m.Hammad Fatma U.Afifi m.lek zihlif Hamzeh J.Al-Ameer mohanad m.Saleh Ismail F.Abaza Zeyad D.Nassar Department of Biology School of ScienceThe University of Jordan Department of Pharmaceutical Sciences School of PharmacyThe University of Jordan Department of Pharmacology School of MedicineThe University of Jordan School of medicine and Freemasons Foundation Centre for men's Health University of Adelaide South Australian Health and medical Research Institute

Objective: To assess the antiangiogcnic activity of ***: Different fractions of fenugreek crude extracts were prepared and their antiangiogenic properties were assessed using the ex vivo rat aortic ring assay and in vivo chicken embryo chorioallantoic membrane(CAm) assay. They were investigated for their direct cytotoxic activity in the mCF7 cells using the mTT ***: The ethanol extract showed 100% inhibition of blood vessel outgrowth from primary tissue explants in the rat aortic ring assay at a concentration of 100μg/mL while the other extracts did not show significant antiangiogenic activity. The ethanol extract was therefore investigated at varying concentrations and exhibited a significant dose dependent effect. The CAm assay coincided with the results of the aortic ring assay as ethanol extract showed a significant inhibition of formation of new blood vessels. The extracts only showed anti-proliferative activity at the highest concentration of 400μg/mL towards mCF7 breast cancer cell lines in the mTT ***: Findings of the both assays confirmed that the ethanol extract inhibited vascularization significantly. Further studies on the ethanol extract would be beneficial in isolating the active ingredient responsible for the inhibition.

关键词： Fenugreek mCF7 Angiogenesis Rat aortic ring assay CAm assay mTT assay

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Screening the RFX6-DNA binding domain for potential genetic variants in patients with type 2 diabetes

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World Journal of Diabetes 2019年第3期10卷 181-187页

作者： Ismail S mahmoud Ayat Homsi Hamzeh J Al-Ameer Jihad Alzyoud mais Darras mohammad Al Shhab m.lek zihlif ma’mon m Hatmal Walhan Alshaer Department of medical Laboratory Sciences Faculty of Allied Health Sciences The Hashemite University Zarqa 13133 Jordan Cell Therapy Centre The University of Jordan Amman 11942 Jordan Department of Pharmacology Faculty of Medicine The University of Jordan Amman 11942 Jordan

BACKGROUND The regulatory factor X6 (RFX6), a member of regulatory factor X family, is known to play a key role in the development and differentiation of pancreatic beta cells as well as insulin production and secretion. However, the potential role of RFX6 in type 2 diabetes (T2D) is still unclear. AIm Recent studies have indicated that RFX6 binding to DNA could be disrupted in diabetes. Therefore, in this study we investigated whether genetic mutations are present in the DNA binding domain of RFX6 gene that could abrogate its function in T2D. mETHODS A cohort of T2D patients was enrolled in this study, and the gene encoding the DNA binding domain of RFX6 was amplified by polymerase chain reaction and then analysed by direct DNA sequencing. RESULTS The DNA sequence analysis revealed the absence of any exonic mutation. However, we have identified a new heterozygous single nucleotide polymorphism (IVS6+31 C>T) in the intronic region of DNA binding domain gene that is present in 9.2% and 8.5% of diabetic and control people, respectively (P = 0.97).CONCLUSION We report the absence of any significant genetic variant that could affect the function of RFX6-DNA binding domain in T2D.

关键词： Regulatory factor X6 Genetic variant Diabetes DNA binding domain

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