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腐蹄病节瘤拟杆菌纤毛蛋白的基因克隆与表达

中国兽医学报 2000年第4期20卷 362-366页

作者：王克坚 Dhungyel om 冯书章吴广谋杨海滨 Egerton Jr 朱平殷震中国农业科学院特产研究所吉林吉林市132109 澳大利亚悉尼大学解放军军需大学军事兽医研究所吉林长春130062

用 PCr技术从腐蹄病 E型节瘤拟杆菌克隆出具免疫保护性抗原 0 .93 kb纤毛蛋白基因 ( Pili基因 ) ,利用该基因构建了纤毛蛋白基因表达载体。先将 PCr产物 Pili基因克隆于 TA Cloning System,扩增后 ,用Eco r 酶切 ,低熔点胶回收 ,经 Kle... 详细信息

用 PCr技术从腐蹄病 E型节瘤拟杆菌克隆出具免疫保护性抗原 0 .93 kb纤毛蛋白基因 ( Pili基因 ) ,利用该基因构建了纤毛蛋白基因表达载体。先将 PCr产物 Pili基因克隆于 TA Cloning System,扩增后 ,用Eco r 酶切 ,低熔点胶回收 ,经 Klenow补平后 ,用 T4DNA连接酶与中间载体 p PLλ连接 ,将 Pili基因克隆于 p PLλ载体 ;经 Bam H 、Bgl 、Pvu 和 Smal +Bgl 酶切鉴定和 p PLλ-Pili重组质粒 Pili基因序列测定正确后 ,扩增 p PLλ-Pili重组质粒 ,用 Bam H 酶切出 2 .1 kb大小的片段 ,回收后 ,与 p mE2 90表达质粒连接 ,转染宿主细胞 PAK/2 pfs,在营养肉汤中进行 Pili基因的表达。培养 1 8～ 2 4 h后 ,离心 ,向上清液中加入 0 .1mol/L mg Cl2 提取重组纤毛蛋白。用羊抗兔 E型节瘤拟杆菌抗血清与提取的重组纤毛蛋白进行对流免疫电泳 ,证明重组纤毛蛋白具有特异性 ;染料结合法测定 1 0 0 0 m L上清液中表达纤毛蛋白粗含量约为 1 1 4mg。SDS-PAGE测定重组纤毛蛋白的表达量占总菌量的 1 1 .8%,Western

关键词：腐蹄病节瘤拟杆菌纤毛蛋白基因克隆

Spatio-Temporal Effect on Soil respiration in Fine-Scale Patches in a Desert Ecosystem

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Pedosphere 2006年第1期16卷 1-9页

作者： S. PEN-mOUrATOV m. rAKHImBAEV Y. STEINBErGEr Faculty of Life Sciences Bar-Ilan University Ramat-Gan 52900 (Israel) Faculty of Life Sciences Bar-Ilan University Ramat-Gan 52900 (Israel) Institute of Botany Tashkent (Uzbekistan)

Soil organisms in terrestrial systems are unevenly distributed in time and space, and often aggregated. Spatiotemporal patchiness in the soil environment is thought to be crucial for the maintenance of soil biodiversity, providing diverse microhabitats tightly interweaving with resource partitioning. Determination of a ＂scale unit＂ to help understand ecological processes has become one of the important and most debatable problems in recent years. A fieldwork was carried out in the northern Negev Desert highland, Israel to determine the influence of fine-scale landscape patch moisture heterogeneity on biogeochemical variables and microbial activity linkage in a desert ecosystem. The results showed that the spatio-temporal patchiness of soil moisture to which we attribute influential properties, was found to become more heterogenic with the decrease in soil moisture availability （from 8.2 to 0.4 g kg^-1） toward the hot, dry seasons, with coefficient of variation （CV） change amounting to 66.9%. Spatio-temporal distribution of organic matter （om） and total soluble nitrogen （TSN） was found to be relatively uniformly distributed throughout the wet seasons （winter and spring）, with increase of relatively high heterogeneity toward the dry seasons （from 0.25% to 2.17% for om, and from 0 to 10.2 mg kg^-1 for TSN） with CV of 47.4% and 99.7% for om and TSN, respectively. Different spatio-temporal landscape patterns were obtained for Ca （CV = 44.6%）, K （CV = 34.4%）, and Na （CV = 92%） ions throughout the study period. CO2 evolution （CV = 48.6%） was found to be of lower heterogeneity （varying between 2 and 39 g CO2-C g^-1 dry soil h^-1） in the moist seasons, e.g., winter and spring, with lower values of respiration coupled with high heterogeneity of Na^＋ and low levels of TSN and organic matter content, and with more homogeneity in the dry seasons （varying between 1 and 50 g CO2-C g^-1 dry soil h^-1）. Our results elucidate the heterogeneity and complexity of desert system habitats

关键词： desert microbial respiration patchiness small-scale habitat soil

Direct Current Interruption with rLC Circuit-convoluted Arc Interaction

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高电压技术 2013年第9期39卷 2166-2172页

作者： Leonid m. Shpanin Noel Y. A. Shammas Sarath B. Tennakoon Gordon r. Jones Jim E. Humphries Joseph W. Spencer Staffordshire University Stafford ST 180AD United Kingdom University of Liverpool Liverpool L69 3G J United Kingdom

Investigations are reported into the use of an electromagnetically convoluted arc,external to a magnetic field(B-field)producing coil,in combination with a parallel r,L,C resonant circuit for interrupting quasi-steady *** order to elucidate the complex interactions between the arc,B-field and r,L,C circuit,the B-field producing coil is energised independently from the current to be interrupted and the r,L,C *** results are presented for the time variation of the currents flowing through the arc gap,the B-field coil and the parallel r,L,C circuit,along with the voltage across the arc *** insight is gained into the role of various effects,which are produced by the complex interactions and which might be used to advantage for direct current interruption.

关键词： rLC电路稳态电流中断相互作用调查报告谐振电路磁场线圈

Processing,microstructure and mechanical properties of bimodal size SiCp reinforced AZ31B magnesium matrix composites

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Journal of magnesium and Alloys 2015年第2期3卷 162-167页

作者： m.J.Shen m.F.Zhang W.F.Ying College of Engineering Shenyang Agricultural UniversityShenyang 110866PR China Liaoning Provincial Gem Quality Supervision and Inspection Center Shenyang 110000PR China College of Chemical Engineering and materials Science Eastern Liaoning UniversityDandong 118003PR China

The bimodal size SiC particulates(SiCp)reinforced magnesium matrix composites with different ratios of micron SiCp and nano SiCp(m-SiCp:N-SiCp=14.5:0.5,14:1,and 13.5:1.5)were prepared by semisolid stirring assisted ultrasonic vibration *** AZ31B alloy and all as-cast SiCp/AZ31B composites were extruded at 350℃ with the ratio of 12:*** characterization of the extruded m14+N1(m-SiCp:N-SiCp=14:1)composite revealed the uniform distribution of bimodal size SiCp and significant grain *** microscopy(om)observation showed that,compared with the m14.5+N0.5(m-SiCp:N-SiCp=14.5:0.5)composite,there are more recrystallized grains in m14+N1(m-SiCp:N-SiCp=14:1)and m13.5+N1.5(m-SiCp:N-SiCp=13.5:1.5)composites,but in comparison to the m13.5+N1.5 composite,the average grain size of the m14+N1 composite is slightly *** evaluation of mechanical properties indicated that the yield strength and ultimate tensile strength of the m14+N1 composite were obviously increased compared with other composites.

关键词： magnesium matrix composite microstructure mechanical properties

metabolic complications of hepatitis C virus infection

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World Journal of Gastroenterology 2021年第13期27卷 1267-1282页

作者： rahul Chaudhari Sherouk Fouda Ashik Sainu Joseph m Pappachan Department of medicine Pennsylvania Hospital of the University of PennsylvaniaPennsylvaniaPA 19104United States School of Health and Biomedical Sciences RMIT UniversityMelbourne VIC 3000Australia Department of Gastroenterology and Hepatology Aster Oman HospitalAl GhubraMuscat OM 133Oman Department of Endocrinology and metabolism Lancashire Teaching Hospitals NHS TrustPreston PR29HTUnited Kingdom Faculty of Science Manchester Metropolitan UniversityManchester M156BHUnited Kingdom Faculty of Biology Medicine and HealthThe University of ManchesterManchester M139PLUnited Kingdom

Hepatitis C virus(HCV) infection is a systemic disease that is implicated in multiple extrahepatic organ dysfunction contributing to its protean manifestations. HCV is associated with diverse extrahepatic disorders including atherosclerosis, glucose and lipid metabolic disturbances, alterations in the iron metabolic pathways, and lymphoproliferative diseases over and above the traditional liver manifestations of cirrhosis and hepatocellular carcinoma. The orchestration between HCV major proteins and the liver-muscle-adipose axis, poses a major burden on the global health of human body organs, if not adequately addressed. The close and inseparable associations between chronic HCV infection, metabolic disease, and cardiovascular disorders are specifically important considering the increasing prevalence of obesity and metabolic syndrome, and their economic burden to patients, the healthcare systems, and society. Cellular and molecular mechanisms governing the interplay of these organs and tissues in health and disease are therefore of significant interest. The coexistence of metabolic disorders and chronic hepatitis C infection also enhances the progression to liver fibrosis and hepatocellular carcinoma. The presence of metabolic disorders is believed to influence the chronicity and virulence of HCV leading to liver disease progression. This com.rehensive review highlights current knowledge on the metabolic manifestations of hepatitis C and the potential pathways in which these metabolic changes can influence the natural history of the disease.

关键词： Chronic hepatitis C infection Insulin resistance metabolic syndrome Cardiovascular diseases Fatty liver Diabetes mellitus

PET Oligom.r Waste to modify CAP Characteristics

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materials Sciences and Applications 2012年第8期3卷 575-579页

作者： L. C. mendes m. L. Dias P. S. C. Pereira L. m. r. Albuquerque Centro de Tecnologia Instituto de Macromoléculas Professora Eloisa Mano-IMA Universidade Federal do Rio de Janeiro-UFRJ Rio de Janeiro Brazil

To develop an improved method of reusing poly(ethylene terephthalate) (PET) waste in the production chain, it was chemically modified with pentaerythritol (PENTE), resulting in PET/PENTE (60/40) oligom.r. This was added to petroleum asphalt cement (CAP) in different proportions—5, 10 and 20 wt%—as a modifier of bitumen characteristics. The mixture was evaluated by thermogravimetry (TG/DTG), differential scanning calorimetry (DSC), infrared spectroscopy (FT-Ir), optical microscopy (om) and the adhesion-peel test. The oligom.r enhanced substantially the asphalt thermal stability-Tonset. om images showed strong compatibility between components and PET’s amorphization. PET oligom.r increased CAP’s wettability and the mixture presented cohesive fracture through the peel test. The mixture has excellent potential as paving material.

关键词： PET Waste Pentaerythritol CAP recycling

Exploratory systematic review and meta-analysis on period poverty

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World Journal of meta-Analysis 2023年第5期11卷 196-217页

作者： Gayathri Delanerolle Xiao-Jie Yang Heitor Cavalini om P Kurmi Camilla mørk røstvik Ashish Shetty LuckySaraswat Julie Taylor Sana Sajid Shanaya rathod Jian-Qing Shi Peter Phiri Department of research&Innovation Southern Health NHS Foundation TrustSouthampton SO303JBUnited Kingdom Nuffield Department of medicine University of OxfordOxford OX37JXUnited Kingdom School of Statistics and mathematics Yunnan University of Finance and EconomicsKunming 650221Yunnan ProvinceChina University College London London WC1E 6BTUnited Kingdom Centre for Intelligent Healthcare Institute of Health and WellbeingCoventry UniversityCoventry CV15LBUnited Kingdom Centre for Contemporary Art University of St AndrewsScotland KY169AJUnited Kingdom Pain management Centre University College London Hospitals NHS Foundation TrustLondon W1T 4AJUnited Kingdom Aberdeen Centre for Women’s Health University of AberdeenAberdeen AB243FXUnited Kingdom School of Nursing University of BirminghamBirmingham B152TTUnited Kingdom Statistics and Data Science Southern University of Science and TechnologyShenzhen 518055Guangdong ProvinceChina School of Psychology Faculty of Environmental and Life SciencesUniversity of SouthamptonSouthampton SO171BJUnited Kingdom

BACKGrOUNDPeriod poverty is a global health and social issue that needs to be *** has been reported that many females com.romise their education,employment,and social commitments during their menstruation days due to a number of reasons,including lack of access to toilets or menstrual *** To provide a com.rehensive understanding on period poverty,including outcomes associated with *** All observational and randomised clinical trials reporting menstruation challenges,menstrual poverty and menstrual products were *** search strategy included multiple electronic databases of Pubmed,Web of Science,ScienceDirect,ProQuest and *** published in a peer review journal in English between the 30th of April 1980 and the 30th of April 2022 were *** Newcastle-Ottawa Scale was used to assess the risk of bias of the systematic included *** odds ratios(Ors)together with 95%confidence intervals(CIs)are reported overall and for *** A total of 80 studies were systematically selected,where 38 were included in the *** the 38 studies,28 focused on children and young girls(i.e.,10-24 years old)and 10 included participants with a wider age range of 15-49 *** prevalence of using disposable sanitary pads was 45%(95%CI:0.35-0.58).The prevalence of menstrual education pre-menarche was 68%(95%CI:0.56-0.82).The prevalence of good menstrual hygiene management(mHm)was 39%(95%CI:0.25-0.61).Women in rural areas(Or=0.30,95%CI:0.13-0.69)were 0.70 times less likely to have good mHm practices than those living in urban *** There was a lack of evidence,especially from low-and middle-income *** research to better understand the scope and prevalence of period poverty should be *** will enable the development of improved policies to increase access to menstrual products and medical support where necessary.

关键词： Period poverty menstruation mental health menstrual education menstrual hygiene

Crystal and electronic structure study of mn doped wurtzite ZnO nanoparticles

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Progress in Natural Science:materials International 2016年第4期26卷 347-353页

作者： O.m.Ozkendir S.Yildirimcan A.Yuzer K.Ocakoglu Department of Energy Systems Engineering Mersin University Tarsus Faculty of Technology Advanced Technology research & Application Center Mersin University Faculty of Engineering Department of Electrical-Electronics Engineering Toros University

The change in the crystal and electronic structure properties of wurtzite ZnO nanoparticles was studied according to mn doping in the powder samples. The investigations were conducted by X-ray Absorption Fine Structure Spectroscopy(XAFS) technique for the samples prepared with different heating and doping processes. Electronic analysis was carried out by the collected data from the X-ray Absorption Near-Edge Structure Spectroscopy(XANES) measurements. Additional crystal structure properties were studied by Extended-XAFS(EXAFS) analysis. Longer heating periods for the undoped wurtzite ZnO samples were determined to own stable crystal geometries. However, for some doped samples, the distortions in the crystal were observed as a result of the low doping amounts of mn which was treated as an impurity. Besides, the changes in oxygen locations were determined to create defects and distortions in the samples.

关键词： Electronic materials Nanostructures Oxides XAFS(EXAFS and XANES) Crystal structure

microstructural evolution and mechanical properties of thixoformed A319 alloys containing variable amounts of magnesium

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Transactions of Nonferrous metals Society of China 2016年第8期26卷 2029-2042页

作者： m.S.SALLEH m.Z.om.r K.S.ALHAWArI m.N.mOHAmmED m.A.mAD ALI E.mOHAmAD Department of manufacturing Process Faculty of Manufacturing Engineering Universiti Teknikal Malaysia Melaka Department of mechanical and materials Engineering Faculty of Engineering and Built EnvironmentUniversiti Kebangsaan Malaysia Department of Engineering and Technology Faculty of Information Sciences and EngineeringManagement and Science University

The effects of mg content on the microstructure and tensile properties of thixoformed A319 alloys were studied. The samples were thixoformed at 50% liquid content and some of the thixoformed samples were subjected to the T6 heat treatment. The samples were then examined by optical microscopy (om), scanning electron microscopy (SEm), energy dispersive X-ray (EDX) spectroscopy and X-ray diffraction (XrD) analysis as well as tensile tests. The results showed that magnesium was able to refine the eutectic silicon in the samples. It was also observed that a compact Al9Femg3Si5 phase was formed when the magnesium content was 1.0% and 1.5%. The results also revealed that as the magnesium content in the alloy increases, the tensile strengths of the thixoformed alloys also increase. The ultimate tensile strength, yield strength and elongation to fracture of the thixoformed A319 heat treated alloy were 298 mPa, 201 mPa and 4.5%, respectively, whereas the values of the thixoformed heat treated alloy with 1.5% mg content were 325 mPa, 251 mPa and 1.4%, respectively. Thixoformed A319 alloy showed a dimple fracture behaviour, while thixoformed A319 alloys with 1.5% mg showed a mixed mode fracture behaviour, where dimple and cleavage ruptures were seen on the fracture surface of the samples.

关键词： aluminium alloy thixoforming T6 heat treatment mechanical properties