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Clonal Fidelity of micropropagated Psidium guajava L. Plants Using microsatellite markers

American Journal of Plant Sciences 2015年第14期6卷 2385-2392页

作者： Bernessa Rawls Karen harris-Shultz Sadanand Dhekney Ivis Forrester Viji Sitther Feagin mill middle School Warner Robins GA USA USDA Crop Genetics and Breeding Research Unit Tifton GA USA Sheridan Research and Extension Center University of Wyoming Sheridan WY USA School of Community Health and Policy Morgan State University Baltimore MD USA Department of Biology Morgan State University Baltimore MD USA

micropropagation of Psidium guajava L. (guava) is a viable alternative to currently adopted techniques for large-scale plant propagation of commercial cultivars. Assessment of clonal fidelity in micropropagated plants is the first step towards ensuring genetic uniformity in mass production of planting material. In the present study, 31 plants of guava cultivar “Lucknow 49” regenerated by micropropagation were tested for genetic fidelity by comparing them to the mother plant from which explant material was obtained. Efficient rooting of in vitro proliferated shoots was obtained by culture on 1/2 strength mS medium supplemented with either 9.8 μm indole butyric acid (IBA) or 11.4 μm indole acetic acid (IAA). Leaf samples of 31 regenerated plants were compared to the mother plant using 17 simple sequence repeat (SSR) markers. While 16 SSRs detected the same allele, locus mPgCIR07 detected slight differences, where six micropropagated plants were 1 bp smaller (152 bp) than the parental genotype (153 bp). Differences in leaf tissues for anthocyanin pigmentation were also noted among micropropagated plants. Results of the study indicated efficient rooting of “Lucknow-49” cultivar for rapid propagation of planting material, and revealed that micropropagated plants were identical for 16 of the 17 loci examined. Although most mutations induced by tissue culture may not have an effect on phenotype, the possibility that novel phenotypes can be generated in a commercial setting exists.

关键词： Genetic Stability Guava Simple Sequence Repeats Tissue Culture

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Eosinophil mBP Extract modulates Oncogene Expression in Prostate Tumor Cells: A Preliminary Study with monolayer Cultures

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Journal of Cancer Therapy 2015年第6期6卷 482-492页

作者： Christine A. Clarke michael A. Smith Ibrahim Laniyan Theresa R. Vaughn Debra Parish-Gause William Green Paulette m. Furbert-harris Department of microbiology Howard University College of Medicine Washington DC USA Howard University Cancer Center Howard University College of Medicine Washington DC USA Carolina’s Hospital System marion Mullins SC USA Department of Pathology Howard University College of Medicine Washington DC USA National Human Genome Center at Howard University Howard University College of Medicine Washington DC USA

Prostate cancer is the second leading cause of cancer deaths in the United States and remains a significant health concern for men throughout the world. Despite the discovery of promising immunotherapeutic strategies, curative outcomes remain elusive. We have investigated eosinophils as potential anti-cancer effector cells, and have reported the ability of their toxic granular proteins (mBP, EPO, ECP, EDN) to inhibit prostate tumor cell growth?in vitro. This study investigates the effect of eosinophil mBP extract on the expression of oncogenes p53, bcl-xl, bax, and c-myc, which modulate tumor growth, proliferation, and apoptosis. Briefly, granular proteins were differentially extracted from GRC.014.22 and GRC.014.24, eosinophilic cell lines established in our laboratory from a patient with moderate asthma. Protein extracts were fractionated on Sephadex G-50 columns, and prostate tumor cell lines DU-145, LNCaP, PC-3, and HPC8L (established in our laboratory from a tumor resected from an African American patient) were treated with mBP extracts from the pooled third peaks. Colony formation and monolayer cell growth inhibition assays were used to evaluate the protein’s growth inhibitory activity against prostate tumor cells;and gene expression analyses, to determine p53, bcl-xl, bax, and c-myc oncogene expression. We show that the granular proteins were potent in their action on HPC8L, inhibiting colony formation in a dose-dependent manner. Treated prostate tumor cell lines trended toward apoptosis-induction, as evident in bcl-xl/bax ratios myc. These preliminary results demonstrate the growth inhibitory potential of eosinophil granular proteins and strongly support the hypothesis that eosinophils modulate the expression of oncogenes associated with prostate tumor proliferation and apoptosis. more importantly, this study offers insights into possible applications of eosinophilic mediators in oncogenic-targeted

关键词： Eosinophils major Basic Protein (mBP) Prostate Cancer HPC8L Oncogenes

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Upgrade of beam energy measurement system at BEPC-Ⅱ

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Chinese Physics C 2016年第7期40卷 90-99页

作者：张建勇蔡啸莫晓虎郭迪舟王建力刘佰奇 m.N.Achasov A.A.Krasnov N.Yu.muchnoi E.E.Pyata E.V.mamoshkina F.A.harris Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Budker Institute of Nuclear Physics Siberian Branch of the Russian Academy of Sciences11 LavrentyevNovosibirsk 630090Russia Novosibirsk State University Novosibirsk 630090Russia University of Hawaii HonoluluHawaii 96822USA

The beam energy measurement system is of great importance for both BEPC-II accelerator and BES-III detector. The system is based on measuring the energies of Compton back-scattered photons. In order to meet the requirements of data taking and improve the measurement accuracy, the system has continued to be upgraded, which involves the updating of laser and optics subsystems, replacement of a view-port of the laser to the vacuum insertion subsystem, the use of an electric cooling system for a high purity germanium detector, and improvement of the data acquisition and processing subsystem. The upgrade system guarantees the smooth and efficient measurement of beam energy at BEPC-II and enables accurate offline energy values for further physics analysis at BES-III.

关键词： laser HPGe detector beam energy measurement

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一种紧凑的534.1nm波长中性锰蒸气激光器

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四川激光 1983年第1期4卷 49-50页

作者： m.CEmCOKAY JAmESS.harris 尹向泽

本文叙述了一种紧凑的高脉冲重复频率的534.1nm波长中性锰蒸气激光器的制作技术。非金属恒温保护层(Shielding)、Vyton O型环状放电管支架,密封、紧凑的结构和自加热技术均已被采用,在频率为10Kmz时平均激光输出功率为300mw,其壁塞效应(... 详细信息

本文叙述了一种紧凑的高脉冲重复频率的534.1nm波长中性锰蒸气激光器的制作技术。非金属恒温保护层(Shielding)、Vyton O型环状放电管支架,密封、紧凑的结构和自加热技术均已被采用,在频率为10Kmz时平均激光输出功率为300mw,其壁塞效应(Wall plug efficiency)为0.2％。

关键词：激光器紧凑蒸气中性高脉冲重复频率波长激光输出功率锰 300mw 制作技术

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前言

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Zoological Research 2014年第S1期35卷 1-2页

作者：詹姆斯·哈里斯国际鹤类基金会

1988年初我第一次在云南纳帕海见到黑颈鹤,那时,黑颈鹤是世界15种鹤类中最鲜为人知的物种。黑颈鹤栖息于高原湿地,在海拔4900 m以上的青藏高原繁殖,在海拔稍低的山谷度过寒冬。这个物种的存在,为地球上最为遥远闭塞的一些地方赋予了生机... 详细信息

1988年初我第一次在云南纳帕海见到黑颈鹤,那时,黑颈鹤是世界15种鹤类中最鲜为人知的物种。黑颈鹤栖息于高原湿地,在海拔4900 m以上的青藏高原繁殖,在海拔稍低的山谷度过寒冬。这个物种的存在,为地球上最为遥远闭塞的一些地方赋予了生机,引起了全世界的关注。

关键词：黑颈鹤鹤类高原湿地纳帕海青藏高原海拔物种栖息

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Determination of Ketoprofen in Human Plasma by RP-HPLC

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American Journal of Analytical Chemistry 2013年第5期4卷 252-257页

作者： Farya Zafar muhammad harris Shoaib Asia Naz Rabia Ismail Yousuf Huma Ali Department of Pharmaceutical Chemistry Faculty of PharmacyUniversity of Karachi Karachi Pakistan Department of Pharmaceutics Faculty of Pharmacy University of Karachi Karachi Pakistan Ziauddin College of Pharmacy Ziauddin University Karachi Pakistan

For in vivo pharmacokinetic studies, it is pre-requisite to quantify drug concentrations in plasma. In the present study a RP-HPLC procedure was developed and validated for the assessment of ketoprofen in human plasma. For this purpose mobile phase consisting of methaol:water (70:30) adjusted to pH 3.3 with phosphoric acid was used, and chromatography was carried out on Discovery HS C18 column, 5 μm (25 cm × 4.6 mm). The flow rate was 1 mL·min-1 and quantitative assessment was performed at 260 nm. The retention time was found to be was found to be accurate and illustrated linearity from 0.2441 to 125 μg·mL-1 with the determination coefficient (r2) of 0.9999, also accuracy and precision were found to be mL-1, 15.625 μg·mL-1, 7.812 μg·mL-1 and 1.953 μg·mL-1 were found to be 99.747%, 99.475%, 98.457% and 99.824% respectively where as for interday accuracy consecutive values for days 1, 2 and 3 were 99.104%, 99.091%, 98.96% and 99.385% in plasma. All validation parameters were assessed and were found to be within the limits. The proposed method was accurate, specific, quick (retention time min), selective (showed no interference with excipients), cost effective and a good resolution which gave this method an advantage over the different other reported methods for the estimation of ketoprofen in human plasma.

关键词： RP-HPLC Ketoprofen Linearity Accuracy Precision Specific

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Synthesis, Crystal Growth and Characterization of Organic NLO material: m-Nitroacetanilide

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Advances in materials Physics and Chemistry 2011年第2期1卷 39-43页

作者： Ramesh Rajendran Thangammal harris Freeda Udaya Lakshmi Kalasekar Rajesh Narayana Peruma 不详

Single crystals of m-Nitroacetanilide (mNAa) were successfully grown by slow evaporation method at a constant temperature 40°C from methanol solution. The solubility studies for mNAa were estimated. The cell dimensions were obtained by single crystal X-ray diffraction (XRD) study. The functional groups have been confirmed using Fourier transform infrared (FTIR) analysis. The placement of protons was identified from Nuclear magnetic Resonance Spectroscopy (NmR) spectral analysis. UV-visible and fluorescence spectral analyses were carried out for the grown crystals. Thermo gravimetric analysis and differential thermal analysis were carried out to determine the thermal properties of the as grown crystal. The Second Harmonic Generation (SHG) efficiency of mNAa was also determined.

关键词： Supersaturated Solution X-Ray Diffraction Single Crystal Growth Organic Compounds Nonlinear Optic materials

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情侣依恋与情感投入:性别的调节作用

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心理科学 2012年第3期35卷 654-658页

作者：陆爱桃张积家 michael harris Bond 张学新华南师范大学心理应用研究中心心理健康与认知科学广东重点实验室广东省突发事件心理援助应急技术研究中心广州510631 香港中文大学心理学系

考察情侣依恋、情感投入和性别之间的关系。结果发现,承诺和满意度与焦虑依恋和逃避依恋相关显著,对两种依恋预测作用显著。性别调节作用仅存在于承诺和满意度与焦虑依恋的关系中:男生的承诺与焦虑依恋关系较强;女生的满意度与焦虑依恋... 详细信息

考察情侣依恋、情感投入和性别之间的关系。结果发现,承诺和满意度与焦虑依恋和逃避依恋相关显著,对两种依恋预测作用显著。性别调节作用仅存在于承诺和满意度与焦虑依恋的关系中:男生的承诺与焦虑依恋关系较强;女生的满意度与焦虑依恋关系较强。承诺和满意度与逃避依恋的关系没有性别差异。焦虑依恋和逃避依恋相对独立。性别对承诺和满意度与焦虑依恋和逃避依恋的关系中有不同调节作用。

关键词：情侣依恋性别承诺满意度

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Human dNK cell function is differentially regulated by extrinsic cellular engagement and intrinsic activating receptors in first and second trimester pregnancy

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Cellular & molecular Immunology 2017年第2期14卷 203-213页

作者： Jianhong Zhang Caroline E Dunk melissa Kwan Rebecca L Jones Lynda K harris Sarah Keating Stephen J Lye Research Centre for Women's and Infants' Health Lunenfeld-Tanenbaum Research Institute Mount Sinai Hospital Toronto ON M5T 3H7 Canada Department of Obstetrics & Gynaecology University of Toronto Toronto ON M5G 1 L4 Canada Department of Physiology University of Toronto Toronto ON M5G 1L4 Canada Institute of Human Development University of Manchester Manchester M 13 9WL UK St. mary's Hospital Central Manchester University Hospitals NHS Foundation Trust Manchester Academic Health Science Centre Manchester M13 9WL UK Department of Pathology University of Toronto Toronto ON M5G 1L4 Canada

Decidual natural killer （dNK） cells express an array of activation receptors to regulate placental immunity and development during early pregnancy. We investigated the functional character of human dNK cells during the first and second trimester of gestation and the interaction between dNK and trophoblast cells. Although the frequency of CD56＋CD16-dNK among the total CD45＋ leukocytes did not change over this period, the expression of the activating receptors, NKp80 and NKG2D, was greatly upregulated. We observed a significantly higher number of extravillous trophoblast cells in proximity to the dNK cells in the first trimester in comparison with the second trimester decidua. NKG2D expression by first trimester dNK cells was decreased when co-cultured with the HTR-8 trophoblast cell line. In the second trimester, functional markers of dNK activation, i.e., angiogenic factor production （e.g., vascular endothelial growth factor, interleukin-8, interferon-gamma）, remained stable despite an increase in NKp80 or NKG2D surface expression. Furthermore, the degranulation capacity of dNK cells, as assessed by CD107a, was decreased in the second trimester. We suggest that in the first trimester, trophoblast-dNK interactions generate a population of dNK cells with a suppressed activating phenotype. In the second trimester, the loss of trophoblast-dNK interactions led to the inhibition of dNK cell function, although their activating receptor expression was increased. We speculate that during pregnancy, two mechanisms operate to modulate the dNK cell activation：suppression of activating receptor levels in the first trimester by trophoblasts and disengagement of receptor-ligand coupling in the second trimester.

关键词： decidual NK cells IFN-gamma NKG2D NKp80 pregnancy

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新西兰怀卡托流域9座大坝的评估--结合资产和大坝安全风险更好地透视管理决策

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大坝与安全 2022年第2期36卷 61-66页

作者： m.Jonathan harris Will Oxley murray Gillon 崔弘毅(翻译) 许传桂(校核) 施纳贝尔工程公司水星有限公司大坝监察工程公司不详

风险知情决策制定方法正越来越多地被用于支持水电项目的资本支出计划。通过使用资产管理工具和策略,可以在整个基础设施组合中综合考虑设备的定期维护、长期修复和资产性能评估。这些工具为决策制定者提供了所需的度量标准,可以通过风... 详细信息

风险知情决策制定方法正越来越多地被用于支持水电项目的资本支出计划。通过使用资产管理工具和策略,可以在整个基础设施组合中综合考虑设备的定期维护、长期修复和资产性能评估。这些工具为决策制定者提供了所需的度量标准,可以通过风险知情流程更有效地使用资本。这些基于风险的工具能让人更好地理解延迟维护或设计及性态评估等概念如何影响所有利益相关者的风险。大坝安全风险评估过程主要关注决策制定的生命安全方面。风险知情决策制定方法的发展使决策者能够了解和比较各种类型的大坝失事风险,其中许多情况都只具有极低的失事可能性,但失事后果非常严重。笔者将讨论风险知情决策制定方法与基于风险的资产管理计划相结合的价值。案例将说明其如何应用到新西兰怀卡托流域9座大坝和水电站的资产组合中。这些水电站位于一条独立的河流上,这加大了理解相互依存风险的复杂性。文章阐述了确定年化成本风险的一般过程,以及如何通过该过程加强对大坝安全和资产管理计划的理解;还阐述了如何将潜在的大坝安全相关影响(如生命安全、环境、企业和第三方的影响)整合到资产管理计划中,以增加对其结构物组合的相对风险的了解。文章概述了所获得的经验教训,以说明进行这种评估的益处。

关键词：水电资产安全风险基于风险的决策

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