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Laser irradiation effects and its possible mechanisms of action on spermatozoa functions in domestic animals

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Asian pacific Journal of Reproduction 2017年第3期6卷 97-103页

作者： Lone SA m.hanty tk Kumaresan A Bhakat m Artificial Breeding Research Centre ICAR-National Dairy Research InstituteKarnal132001HaryanaIndia Livestock Research Centre ICAR-National Dairy Research InstituteKarnal132001HaryanaIndia

This article presents a review pertains the laser irradiation effects and its possible mechanisms of action on spermatozoa functions in domestic animals. To improve artificial insemination, laser is sensitive and cost effective technique, when compared to other conventional methods. Laser may have both positive and negative effects on spermatozoa functions. Since the effects of light are mediated by reactive oxygen species, and the levels of these reactive oxygen species following irradiating spermatozoa with laser may be responsible for determining the effects of laser on sperm. Dose of laser may be regarded as of great significance and this dosage of laser may be responsible for determining its effects on spermatozoa. Optimum dosage of laser for improving seminal attributes may vary among various species and this need to be standardized in each of them. The beneficial effects include improving sperm livability, acrosomal integrity, hypo-osmotic swelling response, mitochondrial function and computer-aided sperm analysis parameters. The increase in cytochrome c oxidase activity, ATP levels and mitochondrial membrane potential, in laser irradiated cells may be responsible for enhanced sperm quality parameters. Improving fertility with laser irradiated spermatozoa has been reported in few species like boar and need to be elaborated in other species. In conclusion laser may be regarded as an easy, cheap and time saving technology for improving artificial insemination;in addition, laser may have various potential applications in the field of reproductive biotechnology as well as in livestock farms and veterinary polyclinics.

关键词： Laser Irradiation Spermatozoa Function mechanism

Q-switched giant pulsed erbium-doped all-fiber laser with V_(2)ZnC mAX phase saturable absorber

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Optoelectronics Letters 2024年第6期20卷 321-329页

作者： Kawther m musthafa Azura Hamzah Ooi Wei Ling Ahmad Haziq Aiman Rosol Norliza mohamed Sulaiman Wadi Harun malaysia-Japan International Institute of Technology Universiti Teknologi MalaysiaKuala Lumpur 54100Malaysia Razak Faculty of Technology and Informatics Universiti Teknologi MalaysiaKuala Lumpur 54100Malaysia Department of Electrical Engineering University of MalayaKuala Lumpur 50603Malaysia1

mXenes,drawn from mAX phases,are special two-dimensional substances with numerous advantages in nonlinear optics,specifically in giant and ultrashort pulsed-laser ***_(3)C_(2)T_(x)and Ti_(2)CT_(x)nanosheets however rapidly deteriorate under ambient conditions,limiting their *** paper demonstrates how excellent modulation depth of one of the mAX phase compounds vanadium zinc carbide(V_2ZnC)makes it a brilliant saturable absorber(SA)in passively Q-switched all-fiber pulsed lasers,integrated such that a 16.73-μm V_(2)ZnC-polyvinyl alcohol(PVA)thin film acts as SA in the *** and non-saturable absorptions were found to be 13.2%and 10.47%,while saturation optical intensity and modulation depth were 6.25 k W/cm^(2)and 12.43%,respectively,illustrating the optical *** superiority of mAX-PVA,fabricated in four distinct ratios,was demonstrated by the fact that it self-starts a giant pulsed laser at pump power as low as 22.5 mW and firmly accomplished 120.6 kHz repetition rate with a pulse width of 2.08μ*** is a fine SA for the use of pulsed-laser production using all-fiber laser due to fabrication simplicity and great optical,thermophysical,and mechanical qualities.

关键词： mAX Q-switched giant pulsed erbium-doped all-fiber laser with V_(2)ZnC mAX phase saturable absorber

青石棉诱导A_L细胞CD59基因突变和DNA氧化损伤的研究

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中华劳动卫生职业病杂志 2004年第1期22卷 43-46页

作者：许安吴李君 Hei tk 余增亮中国科学院等离子体物理研究所离子束生物工程学重点实验室合肥230031 Center for Radiological Research College of Physicians and SurgeonsColumbia Universitynewyork10032

目的　研究谷胱甘肽合成酶抑制剂buthioninesulfoximine(BSO)和自由基清除剂二甲亚砜 (DmSO)对青石棉诱导人鼠杂交瘤 (AL)细胞CD59基因突变率的影响以及青石棉引发细胞内 8 羟基脱氧鸟苷 (8 OHdG)产生的规律。方法　细胞毒性、遗传毒性... 详细信息

目的　研究谷胱甘肽合成酶抑制剂buthioninesulfoximine(BSO)和自由基清除剂二甲亚砜 (DmSO)对青石棉诱导人鼠杂交瘤 (AL)细胞CD59基因突变率的影响以及青石棉引发细胞内 8 羟基脱氧鸟苷 (8 OHdG)产生的规律。方法　细胞毒性、遗传毒性检测分别采用克隆法 ;8 OHdG检测采用ABC酶免疫染色法 ;非蛋白巯基化合物 (NPSH)检测采用改进后的Tietze法。结果　 2 5μmol/LBSO预处理AL 细胞 2 4h后的细胞内NPSH含量降为 2nmol/ 10 7细胞 ,仅为对照组的 5%。青石棉单独处理组AL 细胞CD59基因突变率为 2 0 8± 18。BSO预处理 2 4h后与青石棉继续共同孵育组细胞突变率可达到 3 97± 55,是青石棉单独处理组的 2倍左右 ,差异有显著性 (P mSO存在时 ,青石棉诱导的CD59基因突变率仅为 57± 8,比青石棉单独处理组降低 72 .6%。细胞内 8 OHdG的产生随着青石棉处理剂量的增加而线性增加 (y =150 + 2 0x ,r =0 .962 1)。当青石棉处理剂量为 6μg/cm2 时 ,细胞内 8 OHdG含量由对照组的 13 7± 9提高到 2 89± 6,是对照组的 2倍以上。DmSO存在时 ,可使 6μg/cm2 石棉诱导的细胞内 8 OHdG含量由 2 89± 6降低到 170± 3。结论　自由基是青石棉诱导基因突变和DNA损伤过程中重要的调节物 ,具有剂量

关键词：青石棉 AL细胞 CD59基因基因突变 DNA氧化损伤 DNA突变脱氧鸟嘌呤核苷酸类致癌因子

A Study of Radiation-Induced Telomere Instability Using multiplex Ligation-Dependent Probe Amplification (mLPA)

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Advances in Biological Chemistry 2024年第3期14卷 73-85页

作者： Alan Chant Ahmad Chaudary Christina m. Kraemer-Chant Department of microbiology and molecular Genetics University of Vermont Burlington VT USA Department of Public and Allied Health Bowling Green State University Bowling Green OH USA Department of Chemistry Saint Michaels College Colchester VT USA

The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (tk6 and Wtk1) in the presence and absence of ionizing radiation was analyzed by multiplex Ligation-Dependent Probe Amplification (mLPA). The tk6 cell line has the native p53 tumor-suppressor gene, whereas Wtk1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by mLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that Wtk1 and tk6 are impacted differently after irradiation, and that each cell line presents its own unique mLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated mLPA profile that are not present in the controls, and two unique probe signals only seen in Wtk1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53.

关键词： Ionizing Radiation multiplex Ligation-Dependent Probe Amplification (mLPA) p53 Tumor Suppressor Telomere Instability

Endoscopic push through tragal cartilage tympanoplasty: A 10-year retrospective review of our technique and outcomes

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World Journal of Otorhinolaryngology 2024年第2期11卷 18-24页

作者： K m Abidur Rahman Khalid majeed Emma Finnegan Ivan Keogh Ear Noseand Throat/Head and Neck SurgeryGalway University HospitalGalway H91 YR71Ireland Academic Department of Otorhinolaryngology and Head and Neck Surgery University of GalwayGalway H91 TK33Ireland

BACKGROUND Endoscopic ear surgery(EES)provides a magnified,high-definition view of the otological surgical *** allows otologists to avoid surgical incisions and associated postoperative *** is an ideal technique for the perfor-mance and teaching of *** To examine the efficacy of total Endoscopic Push Through Tragal Cartilage Tympanoplasty(EPTTCT),at our institution over a 10-year *** A retrospective analysis of 168 cases of EPTTCT for closure of small to medium tympanic membrane perforations from 2013-2023 was *** sex,age range(pediatric vs adult),etiology of injury,success rate,complications,and postoperative hearing status were *** Graft uptake results indicated success in 94%of patients,with less than a 2%complication *** pure tone audiometry demonstrated hearing status improvement in 69%of *** EPTTCT has been shown to be effective in tympanic membrane perforation closures with minimal *** study further demonstrates the efficacy and safety of these procedures in a single-center review.

关键词： Total endoscopic ear surgery Otology Tympanoplasty Hearing Perforation Tragal cartilage graft

Nitrosamines crisis in pharmaceuticals-Insights on toxicological implications,root causes and risk assessment:A systematic review

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Journal of Pharmaceutical Analysis 2024年第5期14卷 639-652页

作者： Hemanth P.R.Vikram Tegginamath Pramod Kumar Gunjan Kumar Narasimha m.Beeraka Rajashree Deka Sheik mohammed Suhail Sandeep Jat Namitha Bannimath Gayatiri Padmanabhan Ravandur S.Chandan Pramod Kumar Bannimath Gurupadayya Department of Pharmaceutical Chemistry JSS College of Pharmacy MysuruJSS Academy of Higher Education and Research(JSSAHER)Mysuru570015India Xenone Healthcare Pvt.Ltd. New Delhi110076India Department of Pharmaceutics JSS College of Pharmacy MysuruJSS Academy of Higher Education and Research(JSSAHER)Mysuru570015India Department of Human Anatomy I.M.Sechenov First Moscow State Medical University(Sechenov University)Moscow119991Russian Federation Department of Pharmacology Raghavendra Institute of Pharmaceutical Education and Research(RIPER)Ananthapuramu515721India Herman B.Wells Center for Pediatric Research Department of PediatricsIndiana University School of MedicineIndianapolisIN46202USA Animal Physiology and Biochemistry Laboratory Department of ZoologyGauhati UniversityGuwahati781014India Department of Pharmacology JSS College of Pharmacy MysuruJSS Academy of Higher Education and Research(JSSAHER)Mysuru570015India Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research(NIPER)-GuwahatiChangsari781101India Department of Pharmacology University of GalwayGalwayH91 TK33Ireland

The presence of N-nitroso compounds,particularly N-nitrosamines,in pharmaceutical products has raised global safety concerns due to their significant genotoxic and mutagenic *** systematic review investigates their toxicity in active pharmaceutical ingredients(APIs),drug products,and pharmaceutical excipients,along with novel analytical strategies for detection,root cause analysis,reformulation strategies,and regulatory guidelines for *** review emphasizes the molecular toxicity of N-nitroso compounds,focusing on genotoxic,mutagenic,carcinogenic,and other physiological ***,it addresses the ongoing nitrosamine crisis,the development of nitrosamine-free products,and the importance of sensitive detection methods and precise risk *** comprehensive overview will aid molecular biologists,analytical scientists,formulation scientists in research and development sector,and researchers involved in management of nitrosamine-induced toxicity and promoting safer pharmaceutical products.

关键词： Carcinogenicity Genotoxicity mutagenicity molecular toxicity Nitrosamine impurities Nitrosamine drug substance-related impurities

利用地理信息系统数据库分析钉螺空间区域的分布

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中华预防医学杂志 1999年第6期33卷 343-345页

作者：周晓农 tk Kristensen 洪青标孙乐平 m Fuentes JB malone 江苏省血吸虫病防治研究所无锡市214064 DanishBilharziasisLaboratoryCharlottenlund Denmark SchoolofVeterinarymedicinemFuentes JBMalone

目的　利用地理信息系统（ＧＩＳ）数据库分析中国大陆钉螺的空间区域分布。方法　收集中国九省３４个种群钉螺等位基因实验所得的种群遗传变异资料以及钉螺易感性实验资料，建立数据库。以世界数字地图为背景，在ＡｒｃＶｉｅｗ３．... 详细信息

目的　利用地理信息系统（ＧＩＳ）数据库分析中国大陆钉螺的空间区域分布。方法　收集中国九省３４个种群钉螺等位基因实验所得的种群遗传变异资料以及钉螺易感性实验资料，建立数据库。以世界数字地图为背景，在ＡｒｃＶｉｅｗ３．０ａ软件上与钉螺的遗传变异和易感性数据库相联。采用ＡｒｃＶｉｅｗ空间分析和ＧＩＳ重叠分析功能进行区域划分。收集１９９５年ＡＶＨＲＲＮＤＶＩ卫星图像组合成流行季节的卫星图像，并重叠各种群钉螺的杂合指数、多态位点百分率和血吸虫感染率分布图，进行图像分类。结果　空间分析结果，将钉螺遗传杂合性指数、多态位点百分率和钉螺感染率的分布图，划分成西部和东部二大区域，各区域内有各种类型。三层分布图与ＡＶＨＲＲＮＤＶＩ卫星图像重叠分析后，显示出西部四川、西部云南、中部江湖洲滩和东南部沿海４个明显不同的区域。结论　首次运用ＧＩＳ分析种群遗传学资料，佐证了中国大陆钉螺种群结构为分裂亚群结构，并可区分出多个亚种的理论。

关键词：钉螺空间区域分布地理信息系统

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脑神经肉样瘤病的影像学征象及鉴别诊断

放射学实践 2011年第9期26卷 1022-1023页

作者： Fenchel m Hauser tk Korn A Ernemann U Horger m 焦豫(译) 漆剑频(校) 不详华中科技大学外国语学院德语系

肉样瘤病为特异性全身系统性疾病，以非干酪性肉芽肿为其病理组织学特征。肉样瘤病常见于肺及淋巴结，也发生于人体其它部位。在头部和脊髓会出现脑/脊膜增厚及增强扫描时的强化改变，且以脑基底部表现明显。在少数情况下，肉样瘤病可... 详细信息

肉样瘤病为特异性全身系统性疾病，以非干酪性肉芽肿为其病理组织学特征。肉样瘤病常见于肺及淋巴结，也发生于人体其它部位。在头部和脊髓会出现脑/脊膜增厚及增强扫描时的强化改变，且以脑基底部表现明显。在少数情况下，肉样瘤病可累及骨骼、神经及脑组织。肉样瘤病可以仅发生于中枢神经系统，但极为少见。肉样瘤病可以侵犯多种器官和组织，合并全身其它器官和组织的改变。病因学肉样瘤病的病因目前并不明确，但T淋巴细胞肉芽肿的形成被认为是其病因，因为患者具有明确的T淋巴细胞抗原反应。分支杆菌和丙酸杆菌的RNA和DNA可以在肉样瘤病肉芽肿中被分离出来，这在肉样瘤病的发病机理中具有重要意义。经尸检病理证实，约25％的患者具有全身系统性肉样瘤病。约5％的患者在生前具有症状和体征，其中也只有10％的患者具有显著的影像学征象。仅限于神经系统而不侵犯其它器官和组织的肉样瘤病少见，发病率低于1％，但其临床症状和体征明显。重要的是，放射科医师应该熟悉和了解脑神经肉样瘤病的影像学检查及其结果。

关键词：肉样瘤病脑神经影像学征象鉴别诊断病理组织学特征全身系统性疾病非干酪性肉芽肿中枢神经系统

^(99m)Tc标记肝胆试剂的药物动力学和应用

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国外医学（放射医学核医学分册） 1991年第5期15卷 224-227页

作者： Gerbail tk Fredrick ET 张继和

自1972年^(99m)Tc-D-青霉素胺作为肝胆显像剂以来,五十年代起一直是评价肝胆功能的主要试剂^(131)I-玫瑰红逐渐被^(99m)Tc标记化合物所代替.^(99m)Tc标记的肝胆显像剂主要有两类:吡哆醛氨基酸类(PYG)和亚胺基二乙酸类(IDA),二者相比,以... 详细信息

自1972年^(99m)Tc-D-青霉素胺作为肝胆显像剂以来,五十年代起一直是评价肝胆功能的主要试剂^(131)I-玫瑰红逐渐被^(99m)Tc标记化合物所代替.^(99m)Tc标记的肝胆显像剂主要有两类:吡哆醛氨基酸类(PYG)和亚胺基二乙酸类(IDA),二者相比,以后者为好,因芳香基因在苯环上的位置不同,分别具有肾脏排泄少,肝脏摄取多,经肝转运时间短,进入肠腔快等优点.

关键词：肝胆显像剂药物动力学同位素