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Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential ant1ip1 an-coronavirus activity

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Signal Transduction and Targeted Therapy 2024年第3期9卷 1186-1198页

作者： minghui Yang Chunhui Li Guoguo Ye Chenguang Shen Huiping Shi Liping Zhong Yuxin Tian mengyuan Zhao Pengfei Wu Abid Hussain Tian Zhang Haiyin Yang Jun Yang Yuhua Weng Xinyue Liu Zhimin Wang Lu gan Qianyu Zhang Yingxia Liu Ge Yang Yuanyu Huang Yongxiang Zhao School of Life Science Advanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyBeijing Institute of TechnologyBeijing 100081China National Clinical Research Center for infectious disease Shenzhen Third People’s HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China Guangdong Provincial Key Laboratory of Tropical Disease Research School of Public HealthSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Targeting Oncology National Center for International Research of Biotargeting TheranosticsGuangxi Key Laboratory of Biotargeting TheranosticsCollaborative Innovation Center for Targeting Tumor Diagnosis and TherapyGuangxi Medical UniversityNanning 530021China CAmS Key Laboratory of Antiviral Drug Research Beijing Key Laboratory of Antimicrobial AgentsInstitute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Emerging and recurrent infectious diseases caused by human coronaviruses(HCoVs)continue to pose a significant threat to global public health *** light of this ongoing threat,the development of a broad-spectrum drug to combat HCoVs is an urgently ***,we report a series of anti-pan-coronavirus ssDNA aptamers screened using Systematic Evolution of Ligands by Exponential Enrichment(SELEX).These aptamers have nanomolar affinity with the nucleocapsid protein(NP)of Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and also show excellent binding efficiency to the N proteins of both SARS,mERS,HCoV-OC43 and-NL63 with affinity KD values of 1.31 to 135.36 *** aptamer-based therapeutics exhibited potent antiviral activity against both the authentic SARS-CoV-2 prototype strain and the Omicron variant(BA.5)with EC50 values at 2.00 nm and 41.08 nm,*** protein docking analysis also evidenced that these aptamers exhibit strong affinities for N proteins of pan-coronavirus and other HCoVs(−229E and-HKU1).In conclusion,we have identified six aptamers with a high pan-coronavirus antiviral activity,which could potentially serve as an effective strategy for preventing infections by unknown coronaviruses and addressing the ongoing global health threat.

关键词： acute threat conclusion

鼻腔微生物多样性对慢性鼻窦炎伴鼻息肉患者手术预后的影响

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中华耳鼻咽喉头颈外科杂志 2024年第8期59卷 828-836页

作者：甘卫刚刘星辰刘锋四川大学华西医院耳鼻咽喉头颈外科成都610041

目的探讨慢性鼻窦炎伴鼻息肉(CRSwNP)患者鼻腔微生物多样性,以及术后复发者鼻腔微生物组学特征和炎性细胞及因子特点,以了解微生物组学因素对CRSwNP手术预后的影响。方法2017年12月至2018年12月期间,招募于四川大学华西医院耳鼻咽喉科... 详细信息

目的探讨慢性鼻窦炎伴鼻息肉(CRSwNP)患者鼻腔微生物多样性,以及术后复发者鼻腔微生物组学特征和炎性细胞及因子特点,以了解微生物组学因素对CRSwNP手术预后的影响。方法2017年12月至2018年12月期间,招募于四川大学华西医院耳鼻咽喉科接受手术治疗的77例CRSwNP患者,其中男34例,女43例,年龄29~76岁。收集患者的鼻腔分泌物和鼻息肉组织。于分泌物中提取鼻腔微生物DNA,基于16S核糖体RNA(16S rRNA)进行高通量测序,检测细菌群落组成。使用Luminex分析鼻息肉组织中白细胞介素(IL)-5、IL-8、IL-17A、IL-17E、IL-18、IL-27和干扰素γ(IFN-γ)等细胞因子。计数外周血和鼻息肉组织中嗜酸粒细胞和中性粒细胞含量。术后随访1年,记录鼻息肉的复发情况,分析鼻息肉复发与炎性因子、炎性细胞计数及鼻腔微生物多样性特征的相关性。对二分类变量采用卡方检验,对连续变量采用mann-Whitney U检验,两组之间平均相对丰度的差异比较采用Wilcoxon秩和检验。结果随访1年时复发患者12例,其中男5例,女7例。复发组与未复发组患者在年龄、性别、哮喘、变应性鼻炎、湿疹等方面的差异无统计学意义。复发组的鼻部症状总分[42.3(30.2,67.1),m(Q 1,Q 3),后同]明显高于未复发组[37.8(29.4,50.3)],Z=-4.930,P<0.001。鼻息肉组织中,复发组患者嗜酸粒细胞[40.83(22.33,102.00)个/HP,HP为高倍视野,后同]和中性粒细胞[30.83(20.33,56.44)个/HP]数量显著高于未复发组[13.72(13.50,48.33)个/HP和18.50(12.00,26.08)个/HP,Z值分别为-6.997、-8.243,P值均<0.001]。复发组IFN-γ、IL-17A、IL-17E、IL-18的表达量明显高于未复发组,但阳性率差异无统计学意义。属水平上,未复发组患者鼻腔棒状杆菌属的平均相对丰度为(11.90±20.31)%,高于复发组的(0.15±0.20)%,但校正后差异无统计学意义(FDR P=0.638)。未复发组葡萄球菌属平均相对丰度为(8.17±27.70)%,低于复发组的(8.99±15.89)%,但校正后差异无统计学意义(FDR P=0.638)。结论CRSwNP患者中性粒细胞介导的炎性反应与鼻息肉复发密切相关。鼻内镜术后鼻息肉的复发可能与保护性微生物丰度的减少和致病微生物数量的增加有关。

关键词：鼻窦炎微生物多样性中性粒细胞炎症手术预后

保温时间对二次炭化型煤微结构的影响与力学-渗流特性强化研究

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煤炭科学技术 2024年第6期52卷 111-122页

作者：甘青青许江彭守建蔡果良耿加波江西理工大学应急管理与安全工程学院江西赣州341000 重庆大学煤矿灾害动力学与控制国家重点实验室重庆400044 中煤科工集团重庆研究院有限公司瓦斯灾害监控与应急技术国家重点实验室重庆400037

物理模拟试验是煤矿瓦斯灾害发生机制与防控的一种有效方法。目前开展煤矿瓦斯灾害物理模拟试验所用型煤材料其力学强度、渗透率与原煤差异性较大,如何改善型煤(BC)的抗压强度、渗透率是型煤成型过程中面临的关键问题。基于此,采用热压... 详细信息

物理模拟试验是煤矿瓦斯灾害发生机制与防控的一种有效方法。目前开展煤矿瓦斯灾害物理模拟试验所用型煤材料其力学强度、渗透率与原煤差异性较大,如何改善型煤(BC)的抗压强度、渗透率是型煤成型过程中面临的关键问题。基于此,采用热压成型方法制作了若干不同保温时间下二次炭化型煤,并采用SEm、NmR、XRD、FTIR、mTS-815岩石力学试验系统和含瓦斯煤热-流-固耦合三轴伺服渗流试验装置对不同保温时间下二次炭化型煤进行测试分析,分析了不同保温时间对其表面形貌、T2谱形态、孔隙度、微晶结构、基本结构单元芳香烃、烷基侧链和各种官能团演化规律,厘清了不同保温时间下二次炭化型煤的力学及渗流特性,并确定最优的保温时间。结果表明:随着保温时间的增加,二次炭化型煤表面粗糙度、孔径逐渐变大,且增至6.7 h后,BC表面有裂隙出现,保温时间越长,裂隙越明显;BC累计孔隙度及中孔孔隙度逐渐增大,而微孔孔隙度逐渐减少;芳香层间距(d_(002))呈先减后增,而微晶直径(L_(a))和微晶高度(L_(c))均先增后减;脂肪链的长度减少,芳香环的缩合程度(A_(ar)/A_(al))呈先增大后减小;单轴抗压强度呈先增大后减小,渗透率呈先减小后增加。在保温时间为5.3 h为最佳保温时间,此时热压BC的单轴抗压强度和弹性模量都最大、泊松比和渗透率最小,其力学强度、渗透率和密度依次为9.85 mPa,1.49×10^(-15)m^(2)和1.127 g/cm^(3)。该研究为提高煤矿瓦斯灾害发生机制与防控基础试验中的真实还原性,有效预防控制煤矿瓦斯灾害事故具有重要的现实指导意义。

关键词：煤矿瓦斯灾害微结构力学特性渗流特性二次炭化型煤

The RNA binding protein EHD6 recruits the m^(6)A reader YTH07 and sequesters OsCOL4 mRNA into phase-separated ribonucleoprotein condensates to promote rice flowering

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molecular Plant 2024年第6期17卷 935-954页

作者： Song Cui Peizhe Song Chaolong Wang Saihua Chen Benyuan Hao Zhuang Xu Liang Cai Xu Chen Shanshan Zhu Xiangchao gan Hui Dong Yuan Hu Liang Zhou Haigang Hou Yunlu Tian Xi Liu Liangming Chen Shijia Liu Ling Jiang Haiyang Wang Guifang Jia Shirong Zhou Jianmin Wan State Key Laboratory for Crop Genetics&Germplasm Enhancement and Utilization Zhongshan Biological Breeding LaboratoryNational Observation and Research Station of Rice Germplasm ResourcesNanjing Agricultural UniversityNanjing 210095China State Key Laboratory for Crop Gene Resources and Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking-Tsinghua Center for Life SciencesBeijing Advanced Center of RNA BiologyPeking UniversityBeijingChina Jiangsu Key Laboratory of Crop Genomics and molecular Breeding Agricultural College of Yangzhou UniversityYangzhou 225009China max Planck Institute for Plant Breeding Research Carl-von-Linne´-Weg 1050829 KolnGermany

N6-methyladenosine(m^(6)A)is one of the most abundant modifications of eukaryotic mRNA,but its comprehensive biological functionality remains further *** this study,we identified and characterized a new flowering-promoting gene,EARLY HEADING DATE6(EHD6),in ***6 encodes an RNA recognition motif(RRm)-containing RNA binding protein that is localized in the non-membranous cytoplasm ribonucleoprotein(RNP)granules and can bind both m^(6)A-modified RNA and unmodified RNA *** found that EHD6 can physically interact with YTH07,a YTH(YT521-B homology)domain-containing m^(6)A *** showed that their interaction enhances the binding of an m^(6)A-modified RNA and triggers relocation of a portion of YTH07 from the cytoplasm into RNP granules through phase-separated *** these condensates,the mRNA of a rice flowering repressor,CONSTANS-like 4(OsCOL4),becomes sequestered,leading to a reduction in its protein abundance and thus accelerated flowering through the Early heading date 1 *** together,these results not only shed new light on the molecular mechanism of efficient m^(6)A recognition by the collaboration between an RNA binding protein and YTH family m^(6)A reader,but also uncover the potential for m^(6)A-mediated translation regulation through phaseseparated ribonucleoprotein condensation in rice.

关键词： N6-methyladenosine m^(6)A EHD6 YTH domain-containing protein phase separation heading date

活动大陆边缘岩浆作用及构造演化——以敦煌地块为例

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岩石学报 2024年第3期40卷 702-718页

作者：甘保平唐菊兴第五春荣西南交通大学地球科学与工程学院成都611756 中国地质科学院矿产资源研究所自然资源部成矿作用与资源评价重点实验室北京100037 西北大学大陆动力学国家重点实验室西北大学地质学系西安710069

大陆边缘弧是汇聚板块边界与俯冲有关的岩浆作用产物,通常记录了造山带弧岩浆作用、造山过程和大陆地壳形成与演化等诸多重要的地质过程。作为中亚造山带中段最南部的构造单元,敦煌地块为传统定义上具有早前寒武纪变质结晶基底的微陆块... 详细信息

大陆边缘弧是汇聚板块边界与俯冲有关的岩浆作用产物,通常记录了造山带弧岩浆作用、造山过程和大陆地壳形成与演化等诸多重要的地质过程。作为中亚造山带中段最南部的构造单元,敦煌地块为传统定义上具有早前寒武纪变质结晶基底的微陆块,其后在古生代时期经历了多期次、多阶段的与中亚造山带造山相关的构造热事件并使其发生再活化,进而产生了一系列广泛的弧岩浆-变质作用事件。然而,对于该陆块古生代弧岩浆作用机制和地壳构造演化历史缺乏系统的研究。本文综合近十多年来对敦煌地块的诸多最新研究进展,系统梳理了古生代岩浆岩岩石组合类型、年代格架、地球化学组成以及同时期变质-沉积构造热事件演化特征,得到以下认识:(1)敦煌地块古生代岩浆作用过程呈现阶段性特征,即幕式岩浆作用,构造位置上从东北部逐渐迁移到南部再折返到中部,大致可划分为五期:~510ma、440~410ma、390~360ma、~330ma和280~245ma,而变质作用事件主要集中在450~360ma;(2)古生代岩浆岩类型主要以钙碱性I型花岗岩、埃达克质岩石、少量S型花岗岩和高钾花岗岩为主,且岩石成分从寒武纪低钾拉斑系列逐渐转变为二叠纪高钾、富硅特征;(3)同位素地球化学特征表明,敦煌地块中-北部寒武纪-泥盆纪发育的与俯冲相关的弧岩浆对新生地壳的生长起了重要贡献,并伴随古老地壳再造事件;而南部泥盆纪-石炭纪岩浆作用则以古老地壳物质重熔为主;(4)基于埃达克质岩石的证据,敦煌地块在古生代时期经历了两次显著的地壳增厚事件,早期可能是与北山南部石板山地体/弧碰撞以及幔源岩浆底侵有关,而晚期可能是与俯冲板片后撤或回卷相关,地壳厚度可达~55km;(5)敦煌地块属于中亚造山带中段最南部一个具有前寒武纪基底的古老微陆块,后期卷入了古亚洲洋南向俯冲相关的造山事件使其被强烈破坏与改造。该微陆块作为古亚洲洋南部的活动大陆边缘弧,被与俯冲作用有关的阶段性弧岩浆底侵、部分熔融、增厚地壳和区域性变质作用等机制改造与活化,产生了阶段性侵位的陆缘弧岩浆作用。这些认识为探究中亚造山带微陆块的起源和造山带的构造演化提供新的约束。

关键词：大陆边缘弧幕式岩浆作用再活化地壳加厚敦煌地块中亚造山带

基于动态分解和角度惩罚距离的高维多目标进化算法

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电子学报 2024年第8期52卷 2773-2785页

作者：王旭健张峰干姚敏立火箭军工程大学陕西西安710025

多个领域的优化可归纳为高维多目标优化问题,高维多目标进化算法是解决此类问题的有效方法,然而该方法普遍存在收敛性和多样性较难平衡的问题.针对此问题,本文提出一种基于动态分解和角度惩罚距离的高维多目标进化算法.该算法基于动态... 详细信息

多个领域的优化可归纳为高维多目标优化问题,高维多目标进化算法是解决此类问题的有效方法,然而该方法普遍存在收敛性和多样性较难平衡的问题.针对此问题,本文提出一种基于动态分解和角度惩罚距离的高维多目标进化算法.该算法基于动态分解将种群分成多个类,此过程无需预先设定参考向量,可根据种群自身分布信息进行分解.之后,基于改进的角度惩罚距离从每类中选择个体,从而平衡收敛性与多样性.此外,设计基于Pareto支配、拐点、m近邻角度三原则的锦标赛匹配选择机制.本文算法与9种高维多目标进化算法在27例高维多目标优化测试题上进行对比实验.实验结果表明,本文算法能有效解决不同类型的高维多目标优化问题,并且在不同目标个数上具有较好的稳定性.

关键词：多目标优化高维多目标优化动态分解角度惩罚距离

Exploring the molecular mechanism of action of curcumin for the treatment of diabetic retinopathy,using network pharmacology,molecular docking,and molecular dynamics simulation

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Integrative medicine Discovery 2024年第8期8卷 1-10页

作者： Yuan-Yuan gan Yan-mei Xu Quan Shu Qi-Zhi Huang Tian-Long Zhou Ju-Fang Liu Wei Yu School of Pharmacy Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Second Affiliated Hospital Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Department of medicine Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Department of Obstetrics and Gynecology Xianning Central HospitalThe First Affiliated Hospital of Hubei University of Science and TechnologyXianning 437000China.

Background:Based on network pharmacology and molecular docking,the present study investigated the mechanism of curcumin(CUR)in diabetic retinopathy ***:Based on the DisGeNET,Swiss TargetPrediction,GeneCards,Online mendelian Inheritance in man,Gene Expression Omnibus,and Comparative Toxicogenomics Database,the intersection core targets of CUR and diabetic retinopathy were *** intersection target was imported into the STRING database to obtain the protein-protein interaction *** to the Database for Annotation,Visualization and Integrated Discovery database,the intersected targets were enriched in Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes *** Cytoscape 3.9.1 is used to make the drug-target-disease-pathway *** mechanism of CUR and diabetic retinopathy was further verified by molecular docking and molecular dynamics ***:There were 203 intersecting targets of CUR and diabetic retinopathy identified.1320 GO entries were enriched for GO functions,which were primarily involved in the composition of cells such as identical protein binding,protein binding,enzyme binding,*** was found that 175 pathways were enriched using Kyoto Encyclopedia of Genes and Genomes pathway enrichment methods,which were mainly included in the lipid and atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,pathways in cancer,*** the molecular docking analysis,CUR was found to have a good ability to bind to the core targets of albumin,IL-1B,and *** binding of albumin to CUR was further verified by molecular dynamics ***:As a result of this study,CUR may exert a role in the treatment of diabetic retinopathy through multi-target and multi-pathway regulation,which indicates a possible direction of future research.

关键词： curcumin diabetic retinopathy network pharmacology molecular docking molecular dynamics simulation

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存在物理约束的时变转动惯量航天器姿态控制

航空动力学报 2024年第1期39卷 160-168页

作者：殷春武甘婷徐琳西安建筑科技大学信息与控制工程学院西安710055 西安创联电气科技(集团)有限责任公司西安710065

对存在角速度和控制输入有界的快速机动航天器姿态控制问题,设计了一种自适应双环姿态跟踪控制器。将虚拟有界角速度作为运动学方程的虚拟控制输入,使姿态控制问题降阶为角速度跟踪问题;构建递归自适应算法估计时变转动惯量及其微分,并... 详细信息

对存在角速度和控制输入有界的快速机动航天器姿态控制问题,设计了一种自适应双环姿态跟踪控制器。将虚拟有界角速度作为运动学方程的虚拟控制输入,使姿态控制问题降阶为角速度跟踪问题;构建递归自适应算法估计时变转动惯量及其微分,并基于障碍李亚普诺夫函数和线性回归算子,设计了角速度跟踪误差有界的变增益自适应姿态控制器。结果表明:该控制策略能使抓捕非合作目标航天器的姿态呈指数收敛到期望轨迹,且收敛轨迹不受外部干扰和抓捕瞬间的强干扰影响;在整个控制过程中,航天器的角速度小于0.4 rad/s,控制力矩小于10 N·m,满足了航天器对角速度和控制输入有界的物理限制。

关键词：姿态控制双环控制障碍李亚普诺夫函数角速度在轨捕获

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The FAST Core Array

Astronomical Techniques and Instruments 2024年第2期1卷 84-94页

作者： Peng Jiang Rurong Chen Hengqian gan Jinghai Sun Boqin Zhu Hui Li Weiwei Zhu Jingwen Wu Xuelei Chen Haiyan Zhang Tao An National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Guizhou Radio Astronomical Observatory Guizhou UniversityGuiyang 550000China Shanghai Astronomical Observatory Chinese Academy of SciencesShanghai 200030China

The Five-hundred-meter Aperture Spherical Radio Telescope(FAST)Core Array is a proposed extension of FAST,integrating 24 secondary 40-m antennas implanted within 5 km of the FAST *** original array design will combine the unprecedented sensitivity of FAST with a high angular resolution(4.3"at a frequency of 1.4 GHz),thereby exceeding the capabilities at similar frequencies of next-generation arrays such as the Square Kilometre Array Phase 1 or the next-generation Very Large *** article presents the technical specifications of the FAST Core Array,evaluates its potential relatively to existing radio telescope arrays,and describes its expected scientific *** proposed array will be equipped with technologically advanced backend devices,such as real-time signal processing systems.A phased array feed receiver will be mounted on FAST to improve the survey efficiency of the FAST Core Array,whose broad frequency coverage and large field of view(FOV)will be essential to study transient cosmic phenomena such as fast radio bursts and gravitational wave events,to conduct surveys and resolve structures in neutral hydrogen galaxies,to monitor or detect pulsars,and to investigate exoplanetary ***,the FAST Core Array can strengthen China's major role in the global radio astronomy community,owing to a wide range of potential scientific applications from cosmology to exoplanet science.

关键词： FAST Radio telescope Interferometry Synthesis array