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繁殖母猪的能量和蛋白质营养(续完)

国外畜牧学（猪与禽） 2001年第6期21卷 18-21页

作者： J.Noblet J.Y.Dourmad m.etienne 法国国家农业科学院

泌乳母猪的营养能量需要量和妊娠母猪一样,用于估计泌乳母猪能量需要量的基本数据见表1.显而易见,最重要的变异因子是母猪的产奶量水平.母猪的产奶量很难测定,通常是通过窝增重来进行估计(Noblet和etienne,1989;表1).引起泌乳母猪奶产... 详细信息

泌乳母猪的营养能量需要量和妊娠母猪一样,用于估计泌乳母猪能量需要量的基本数据见表1.显而易见,最重要的变异因子是母猪的产奶量水平.母猪的产奶量很难测定,通常是通过窝增重来进行估计(Noblet和etienne,1989;表1).引起泌乳母猪奶产量及进而引起泌乳母猪的能量需要量发生变化的因素可见etienne等(1999)及Noblet(1999)的综述.图2所示为能量需要量随窝重增长而发生的变化.窝增重从每天2000克增加到每天3000克,母猪代谢能需要量将从每天80兆焦增至每天105兆焦,或者说相当于常规日粮从每天6.5千克增加到每天8.5千克.

关键词：繁殖母猪能量蛋白质营养

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繁殖母猪的能量和蛋白质营养

国外畜牧学（猪与禽） 2001年第5期21卷 15-18页

作者： J.Noblet J.Y.Dourmad m.etienne 法国国家农业科学院

前言研究报道表明,母猪的利用年限与其生产率在逻辑上呈正相关,其利用年限及其有关的问题(返情率,受胎率)一方面取决于母猪的整个繁殖周期中躯体组成变化的特点,更重要的是取决于泌乳期间母猪瘦肉和(或)脂肪组织的减少量(King,1987;Whit... 详细信息

前言研究报道表明,母猪的利用年限与其生产率在逻辑上呈正相关,其利用年限及其有关的问题(返情率,受胎率)一方面取决于母猪的整个繁殖周期中躯体组成变化的特点,更重要的是取决于泌乳期间母猪瘦肉和(或)脂肪组织的减少量(King,1987;Whittemore和morgan,1990).为达到最高繁殖性能的最佳饲养方案,是使母猪躯体成分在繁殖周期任何阶段的变化减少到最低限度(Dourmad等,1994).这样的方案要求有充足的饲料供应以满足整个繁殖周期母猪营养需要.本文目的在于对妊娠及泌乳母猪的能量与蛋白质营养方面作一综述,同时提供一些在实际条件下预测营养需要量的原则,本文将讨论使用析因法进行预测.本文还将探讨气候环境(如环境温度)对泌乳母猪的能量需要量,尤其是对采食量的影响.

关键词：生长猪能量需要量日粮纤维成年母猪经产母猪能量消化率妊娠母猪怀孕母猪代谢能生物能非必需氨基酸理想蛋白质兆焦赖氨酸水平可消化赖氨酸消化能增重子宫组织营养

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Hydrolytic and Antimicrobial Activities of Bacteria Isolated from Fermented Peppers Sold in markets at Brazzaville, Republic of the Congo

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Journal of Biosciences and medicines 2024年第9期12卷 215-234页

作者： Faly Armel Soloka mabika Elgie Viennechie Gatsé Ngo Itsouhou Tarcisse Baloki Ngoulou mérite Berthelie mouanda Nzoussi Nguimbi etienne Laboratory of Cell and molecular Biology Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of the Congo Unit of molecular microbiology and Bioinformatics Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of the Congo

microorganisms are omnipresent in all environments and play mainly the role of transformers, thanks to the multiple enzymes they are able to produce. In order to valorize fermented foods in the Republic of the Congo, this work aimed to characterize and study some properties of microorganisms isolated from samples of peppers sold in three markets of Brazzaville. A numeration of the total aerobic mesophilic flora (TAmF) was made in a solid medium, allowing the evaluation of each sample’s microbial concentration. The microbial mass varied from 2.8 × 105 CFU/g for the Ouénzé sample to 1.8 × 104 CFU/g for the Total sample and 2 × 104 CFU/g for the moungali market sample. The evaluation of the enzymatic properties of the Bacillus isolates showed that 68.42% were capable of producing cellulases and 78.94% were capable of producing amylases and proteases. Antimicrobial activities revealed that 63.15% of the isolates were able to secrete inhibitory substances against E. coli and Staphylococcus aureus. molecular analysis by PCR amplification, sequencing of the 16S rRNA gene and BLAST bioinformatics analysis provides newly identified bacteria strains with new accession numbers in GenBank: Bacillus thuringiensis mBCBR322 (OP474008), Bacillus megaterium mBCBJ1822 (OP476493), Bacillus thuringiensis mBCBR222 (OP476494), Priestia megaterium mBCBJ2022 (OP476495) and Lactobacillus paraplantarum mBCBR1522 (OP476496). multiple sequences alignment of identified sequences with their homologs of GenBank has shown high similarities. The phylogenetic inference assay has provided the two groups of strains observed in this study, and the two groups are very coherent with the phylogeny of the reference.

关键词： Antimicrobial Activity Hydrolytic Activity Sequencing Pepper Brazzaville

Vaccination and measles Epidemic in the Republic of Congo

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Open Journal of Epidemiology 2024年第1期14卷 45-55页

作者： Léa Gwladys Gangoue Yanne mavougou Fabien Rock Niama Pembe Issamou mayengue etienne Nguimbi National Public Health Laboratory (LNSP) Ministry of Health and Population Brazzaville Republic of Congo Cellular and molecular Biology Laboratory (BCm) Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of Congo

measles is a highly infectious, vaccine-preventable viral disease. Often forgotten, it is potentially fatal. Its main symptoms are: fever, cough, conjunctivitis, rhinitis and skin rash. Serological diagnoses by ELISA test based on the detection of anti-measles virus immunoglobulins m and G (Igm and IgG), are used respectively for the confirmation of suspected cases notified by means of clinical signs of the disease in health structures of the twelve departments of the Republic of Congo involved in the epidemiological surveillance of the disease, and for the evaluation of the immunity conferred by vaccination. During 2021, 459 suspected cases of measles were reported and sampled throughout the country, despite the administration under the Expanded Routine Immunization Program (EPI), of 2 doses of the combined measles-rubella vaccine (RR1 and RR2) in 2020. The notification rate was higher in the south of the country, more precisely in the health structures of the departments of Pointe-Noire (42%) and Brazzaville (11%). During the year 2021, 459 suspected cases of measles were notified and sampled throughout the country with a high notification rate in the south of the country, more precisely in the health structures of the Pointe-Noire departments (42%) and Brazzaville (11%). The samples consisting of human blood (serum) were sent to the National Public Health Laboratory and analyzed by various ELISA tests for the detection of anti-measles immunog-lobulins m and G. The analysis of the results obtained shows that the measles virus circulated in all departments of the country. 154 cases (33.55%) were confirmed positive by Igm ELISA and 98 positive cases (63.63%) were patients vaccinated against measles. Vaccination coverage in RR1 of [50%-95% [(first dose) as well as the lowest RR2 mic could be the cause of the high frequency of cases positive vaccinated. The non-detection of IgG immunoglobulins in vaccinated pat

关键词： Vaccination measles Epidemic

Hepatocyte cryopreservation:Is it time to change the strategy?

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World Journal of Gastroenterology 2010年第1期16卷 1-14页

作者： Xavier Stéphenne mustapha Najimi etienne m Sokal Catholic University of Louvain and St Luc Clinics Paediatric Department(HPED)PEDI unitLaboratory of Paediatric Hepatology and Cell Therapy

Liver cell transplantation presents clinical benefit in patients with inborn errors of metabolism as an alternative,or at least as a bridge,to orthotopic liver *** success of such a therapeutic approach remains limited by the quality of the transplanted *** remains the best option for long-term storage of hepatocytes,providing a permanent and sufficient cell ***, isolated adult hepatocytes are poorly resistant to such a process,with a significant alteration both at the morphological and functional ***,the aim of the current review is to discuss the state of the art regarding widely-used hepatocyte cryopreservation protocols,as well as the assays performed to analyse the post-thawing cell quality both in vitro and in vivo. The majority of studies agree upon the poor quality and efficiency of cryopreserved/thawed hepatocytes as compared to freshly isolated *** ice formation or exposure to hyperosmotic solutionsremains the main phenomenon of cryopreservation process,and its effects on cell quality and cell death induction will be *** increased knowledge and understanding of the cryopreservation process will lead to research strategies to improve the viability and the quality of the cell suspensions after *** strategies,such as vitrification,will be discussed with respect to their potential to significantly improve the quality of cell suspensions dedicated to liver cell-based therapies.

关键词： Hepatocyte Cryopreservation Quality mitochondria Intracellular ice formation

Expectations and Level of Satisfaction of the Patient with Parkinson’s Disease Undergoing Deep Brain Stimulation Surgery at the National Institute of Neurology and Neurosurgery

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Advances in Parkinson's Disease 2024年第1期13卷 1-7页

作者： Paola Bazán-Rodríguez Eduardo Ichikawa-Escamilla etienne Reséndiz-Henríquez Carlos E. martínez-Cortés Amin Cervantes-Arriaga mayela Rodríguez-Violante Lisette Bazán-Rodríguez Laboratorio de Enfermedades Neurodegenerativas en el Instituto Nacional de Neurología y Neurocirugía manuel Velasco Suarez Ciudad de México México Laboratorio de Enfermedades Neurodegenerativas en el Instituto Nacional de Neurología y Neurocirugía manuel Velasco Suarez Ciudad de México México. Unidad Periférica para el Estudio de la Neuroinflamación en Patologías Neurológicas del Instituto de Investigaciones Biomédicas en el Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suarez Ciudad de México México

Background: Deep brain stimulation (DBS) is an established treatment for patients with advanced Parkinson’s disease (PD). Reports show continued patient satisfaction after surgery despite not maintaining clinical improvement as measured by evolution scales. Objectives: The present study sought to explore expectations and level of satisfaction in patients after DBS surgery with a semi-structured questionnaire and subsequent correlation with functional scales, Quality of Life (QoL), and motor and non-motor symptoms. methods: We performed descriptive statistics to represent demographic data, Wilcoxon rank tests to determine significant differences, and Spearman correlation between the applied scales. Results: We evaluated 20 patients with a history of DBS surgery. 45% were female, with a mean age of 55.7 ± 14.15 years, a mean disease duration of 13.42 ± 8.3 years, and a mean time after surgery of 3.18 ± 1.86 years. Patients reported surgery meeting expectations in 85.5% and continued satisfaction in 92%. These two variables showed a significant correlation. Conclusions: This sample of patients remained satisfied after DBS surgery, although we found no differences in motor and non-motor clinimetric scales. Further studies are needed to confirm the importance of assessing quality of life in patients with DBS.

关键词： Parkinson’s Disease Quality of Life Deep Brain Stimulation Patient Satisfaction

Fluid Flow Estimation with multiscale Ensemble Filters Based on motion measurements Under Location Uncertainty

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Numerical mathematics(Theory,methods and Applications) 2013年第1期6卷 21-46页

作者： Sébastien Beyou Thomas Corpetti Sai Gorthi etienne mémin INRIA/FLUmINANCE 35042 Rennes CedexFrance CNRS/LETG-Rennes-Costel Campus Villejean Place du recteur Henri Le Moal CS *** Rennes cedexFrance Indian Institute of Space Science and Technology Valiamala P.O.Thiruvananthapuram–695547Kerala India

This paper proposes a novel multi-scale fluid flow data assimilation approach,which integrates and complements the advantages of a Bayesian sequential assimilationtechnique, the Weighted Ensemble Kalman filter (WEnKF) [27]. The data assimilation proposed in this work incorporates measurement brought by an efficient multiscalestochastic formulation of the well-known Lucas-Kanade (LK) estimator. This estimatorhas the great advantage to provide uncertainties associated to the motion measurements at different scales. The proposed assimilation scheme benefits from this multiscale uncertainty information and enables to enforce a physically plausible dynamicalconsistency of the estimated motion fields along the image sequence. Experimentalevaluations are presented on synthetic and real fluid flow sequences.

关键词： Data assimilation stochastic filter particle filters fluid motion estimation

Reconstructing f(T) modified gravity from ECHDE and ECNADE models

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Communications in Theoretical Physics 2020年第9期72卷 75-88页

作者： A E Godonou Ines G Salako m J S Houndjo etienne Baffou Joel Tossa Institut de mathematiques et de Sciences Physiques(ImSP) 01 BP 613 Porto-NovoBenin Ecole de Genie Rural(EGR) 01 BP 55 KetouBenin Faculte des Sciences et Techniques de Natitingou(FAST) BP 72NatitingouBenin

We investigate alternative candidates to dark energy(DE) that can explain the current state of the Universe in the framework of the generalized teleparallel theory of gravity f(T), where T denotes the torsion scalar. To achieve this, we carry out a series of reconstructions taking into account the ordinary and entropy-corrected versions of the holographic and new agegraphic DE *** models are used as alternatives to DE in the literature in order to describe the current state of our Universe. It is remarked that the proposed models indicate behavior akin to phantom or quintessence models. Furthermore, we also generate the parameters of the equation of state associated with entropy-corrected models and we observe a phase transition between the quintessence state and phantom state as it is shown by the recent observational data. We also investigate the stability of these models and we create the {r-s} trajectories and compare with the ΛCDm limit. The behavior of certain physical parameters such as the speed of sound and the Statefinder diagnostic pair {r-s} is compatible with the current observational data.

关键词： teleparallel gravity ECHDE ECNADE

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molecular Detection of Pathogens Involved in Sexually Transmitted Infections in Brazzaville, Congo

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Open Journal of medical microbiology 2024年第3期14卷 190-205页

作者： Jeanine mireille Sita maboundou Bitsi Nina Esther Ngoyi Ontsira Edith Sophie Kombo Bayonne Tanguy mieret Geril Sekangue Obili Herlen Tsoumou Lina Foungou Kessengue Samantha Potokoue mpia Chloé Dupont Guilhem Conquet Gickel mpika Bitsene Bienvenu Roland Ibara Ossibi Axel Aloumba etienne Nguimbi Sylvain Godreuil Faculty of Sciences and Technology Marien NGOUABI University Brazzaville Republic of the Congo Faculty of Health Sciences Marien NGOUABI University Brazzaville Republic of the Congo Bacteriology-Virology Laboratory University Hospital Center Brazzaville Republic of the Congo Department of Infectious Diseases Referral Hospital Center of Talanga Brazzaville Republic of the Congo Parasitology-mycology Laboratory University Hospital Center Brazzaville Republic of the Congo Department of Gynecology-Obstetrics University Hospital Center Brazzaville Republic of the Congo UmR IRD224-CNRS5290-Um mIVEGEC Infectious Diseases and Vectors: Ecology Genetics Evolution and Control CREES (Reshearch Center in Ecology and Evolution Health) Montpellier France Bacteriology Laboratory Arnaud de Villeneuve University Hospital CHU of Montpellier Montpellier France Department of Infectious Diseases University Hospital Center Brazzaville Republic of the Congo

The objective of this multicentric study was to assess the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, mycoplasma génitalium and Trichomonas vaginalis infections in Brazzaville, in the Republic of Congo, using molecular methods. From January to December 2021, the sexually transmitted disease risk participants were recruited from six centers: The Association of Young HIV-Positive People of Congo, The Congolese Association for Family Welfare, The Association for Support to Vulnerable Groups, Talangaï hospital, Brazzaville university hospital (outpatient service) and the private clinic COGEmO (outpatient service). The real-time multiplex PCR was carried out to detect these pathogens. Each patient had at least one specimen (urine, urethral, anal and/or vaginal samples). The patients were considered infected when one of their samples was positive. 287 participants made of 227 women and 60 men were tested. The general prevalence of these infections was: Chlamydia trachomatis 2.79%, Neisseria gonorrhoeae 3.14%, mycoplasma génitalium 3.45% and Trichomonas vaginalis 2.97. The prevalence rates according to sex were: C. trachomatis, m. génitalium, N. gonorrhea and T. vaginalis were 1.32%, 2.05%, 1.32% and 3.42% in women and 8.33%, 7.02%, 10% and 1.75% in men, respectively. most infected patients were asymptomatic. Prevalence rates were higher in bisexual individuals, with the exception of T. vaginalis which showed higher prevalence in heterosexual patients. The bisexual and homosexual individuals represent a major public health problem in sexually active young adults, particularly among men having sex with men. These sexually transmitted infections are mainly asymptomatic, their diagnosis and management remain difficult in developing countries.

关键词： Prevalence Chlamydia trachomatis Neisseria gonorrhoeae mycoplasma génitalium Trichomonas vaginalis