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Identifying damage caused by the 2008 Wenchuan earthquake from VHR remote sensing data

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International Journal of Digital Earth 2009年第4期2卷 309-326页

作者： D.Ehrlicha H.D.Guo K.molch J.W.ma m.pesaresi European Commission-Joint Research Centre Via E.Fermi 2749Ispra21027 Italy Center for Earth Observation and Digital Earth-Chinese Academy of Sciences Beijing100190 China

The paper discusses the potential of very high resolution(VHR)satellite imagery for post-earthquake damage assessment in comparison with the role of aerial ***-disaster optical and radar satellite data are assessed for their ability to resolve collapsed buildings,destroyed transportation infrastructure,and specific land cover *** VHR imagery has shown to be effective in quantifying building stock and for assessing damage at the building *** synthetic aperture radar(SAR)imagery requires further research to identify optimum information extraction procedures for rapid assessment of affected *** on current technical and operational capabilities increasing efforts should be devoted to the generation of spatial datasets for disaster preparedness.

关键词： damage assessment earthquake Sichuan settlements VHR SAR

The Generalised Settlement Area:mapping the Earth surface in the vicinity of built-up areas

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International Journal of Digital Earth 2020年第1期13卷 45-60页

作者： A.J.Florczyk m.melchiorri J.Zeidler C.Corbane m.Schiavina S.Freire F.Sabo P.Politis T.Esch m.pesaresi European Commission Joint Research Centre(JRC)Directorate for SpaceSecurity&MigrationIspraItaly Piksel S.r.l MilanoItaly German Aerospace Center(DLR) German Remote Sensing Data Center(DFD)WeßlingGermany Arhs Developments Italia S.r.l.milano Italy Arhs Developments S.A. LuxembourgLuxembourg

Geo-information on settlements from Earth Observation offers a base for objective and scalable monitoring of the evolution of cities and settlements,including their location,extent and other *** this work,we deploy the best available global knowledge on the presence of human settlements and built-up structures derived from Earth Observation to advance the understanding of the human presence on *** start from a concept of Generalised Settlement Area to identify the Earth surface within which any built-up structure is *** further characterise the resulted map by using an agreement map among the state of the art of remote sensing products mapping built-up areas or other strictly related semantic abstractions as urban areas or artificial *** agreement map is formed by a grid of 1 km2,where each cell is classified according to the number of EO-derived products reporting any positive occurrence of the abstractions related to the presence of built-up *** paper describes the characteristics of the Generalised Settlement Area,the differences in the agreement map across geographic regions of the world,and outlines the implications for potential users of the EO-derived products used in this study.

关键词： Generalised settlement area built-up area human settlement map agreement map

Validation of EO-derived information for crisis management: a Digital Earth perspective in the VALgEO expert community

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International Journal of Digital Earth 2011年第S1期4卷 1-7页

作者： Corbane, C. pesaresi, m. Carrion, D. European Commission Joint Research Centre Italy

New generation remote sensing technologies today open new application areas and demonstrate their effectiveness providing geo-information in support of disaster *** the same time,new challenges can be identified and among them the validation of geo-information under the new specific constraints dictated by the disaster management application area has a crucial *** the frame of the Digital Earth concept,geo-information is a sharable resource,and a resource is truly shareable not only if we know the formal code used to make itand then we can read itbut alsoand in particular during disasterif we know the reliability of this *** is crucial in order to avoid information overflow and time wasting during the decision-making process in support of disaster ***‘International workshop on validation of geo-information products for crisis management,’VALgEO 2009,was held at the Joint Research Centre,in Ispra,Italy on 2123 November *** purpose of this first workshop was to initiate a dialog between the main actors of Emergency Response Services around validation and to raise awareness of the necessity for an independent formal assessment of geoinformation for disaster *** special issue includes a collection of invited papers presented at the *** gathers interdisciplinary initiatives where the majority of the contributions either directly or implicitly address different aspects of validation of geo-information generated following a disaster,ranging from flood information to forest fires and earthquake damage assessments.

关键词： disaster Earth flood

Peptide aptamers:The versatile role of specific protein function inhibitors in plant biotechnology

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Journal of Integrative Plant Biology 2015年第11期57卷 892-901页

作者： monica Colombo Chiara mizzotti Simona masiero martin m.Kater Paolo pesaresi Research and Innovation Centre Fondazione Edmund MachSan Michele all'Adige(Trento) Department of Biosciences University of Milan

In recent years, peptide aptamers have emerged as novel molecular tools that have attracted the attention of researchers in various fields of basic and applied science, ranging from medicine to analytical chemistry. These artificial short peptides are able to specifically bind, track, and inhibit a given target molecule with high affinity, even molecules with poor immunogenicity or high toxicity, and represent a remarkable alternative to antibodies in many different applications. Their use is on the rise, driven mainly by the medical and pharmaceutical sector. Here we discuss the enormous potential of peptide aptamers in both basic and applied aspects of plant biotechnology and food safety. The different peptide aptamer selection methods available both in vivo and in vitro are introduced, and the most important possible applications in plant biotechnology are illustrated. In particular, we discuss the generation of broad- based virus resistance in crops, ＂reverse genetics＂ and aptasensors in bioassays for detecting contaminations in food and feed. Furthermore, we suggest an alternative to the transfer of peptide aptamers into plant cells via genetic transformation, based on the use of cell-penetrating peptides that overcome the limits imposed by both crop transformation and Genetically modified Organism commercialization.

关键词： Biosensors functional genomics peptide aptamers resistance

PGR5-PGRL1-Dependent Cyclic Electron Transport modulates Linear Electron Transport Rate in Arabidopsis thaliana

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molecular Plant 2016年第2期9卷 271-288页

作者： marjaana Suorsa Fabio Rossi Luca Tadini mathias Labs monica Colombo Peter Jahns martin m. Kater Dario Leister Giovanni Finazzi Eva-mari Aro Roberto Barbato Paolo pesaresi molecular Plant Biology Department of Biochemistry University of Turku 20014 Turku Finland Dipartimento di Bioscienze Unlversit~ degli studi di Milano 20133 Milano Italy Plant molecular Biology (Botany) Department Biology I Ludwig-Maximilians-Universit~t MSnchen 82152 Planegg-Martinsded Germany Centro Ricerca e Innovazione Fondazione Edmund Mach 38010 San Michele all'Adige Italy Plant Biochemistry Heinrich-Heine-University Dusseldorf Universitstsstrasse 140225 Dusseldorf Germany Laboratoire de Physiologie Cellulaire & Vegetale Unite Mixte de Recherche 5168 Centre National de la Recherche Scientifique 38054 Grenoble France Dipartimento di Scienze dell'Ambiente e della Vita Universita del Piemonte Orientale viale Teresa Michel 11 15121 Alessandria Italy These authors contributed equally to this article

Plants need tight regulation of photosynthetic electron transport for survival and growth under environ- mental and metabolic conditions. For this purpose, the linear electron transport （LET） pathway is supple- mented by a number of alternative electron transfer pathways and valves. In Arabidopsis, cyclic electron transport （CET） around photosystem I （PSI）, which recycles electrons from ferrodoxin to plastoquinone, is the most investigated alternative route. However, the interdependence of LET and CET and the relative importance of CET remain unclear, largely due to the difficulties in precise assessment of the contribution of CET in the presence of LET, which dominates electron flow under physiological conditions. We there- fore generated Arabidopsis mutants with a minimal water-splitting activity, and thus a low rate of LET, by combining knockout mutations in Psb01, PsbP2, PsbQ1, PsbQ2, and PsbR loci. The resulting 45 mutant is viable, although mature leaves contain only ~20% of wild-type naturally less abundant Psb02 protein. 45 plants compensate for the reduction in LET by increasing the rate of CET, and inducing a strong non-photochemical quenching （NPQ） response during dark-to-light transitions. To identify the molecular origin of such a high-capacity CET, we constructed three sextuple mutants lacking the qE component of NPQ （45 npq4-1）, NDH-mediated CET （45 crr4-3）, or PGR5-PGRLl-mediated CET （45 pgrS）. Their analysis revealed that PGR5-PGRLl-mediated CET plays a major role in ~pH formation and induction of NPQ in C3 plants. moreover, while pgr5 dies at the seedling stage under fluctuating light conditions, 45 pgr5 plants are able to survive, which underlines the importance of PGR5 in modulating the intersystem electron transfer.

关键词： Arabidopsis Photosynthesis Linear Electron Transport Cyclic Electron Transport Oxygen EvolvingComplex PGR5

Update on Chloroplast Research： New Tools, New Topics, and New Trends

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molecular Plant 2011年第1期4卷 1-16页

作者： Ute Armbruster Paolo pesaresi mathias Pribil Alexander Hertle Dario Leister Lehrstuhl for molekularbiologie der Pflanzen (Botanik) Department Biologie I Ludwig-Maximilians-Universitat M6nchen Groβhaderner Str. D-82152 Planegg-martinsried Germany b Dipartimento di Scienze Biomolecolari e Biotecnologie Universita degli studi di Milano 1-20133 Milano Italy

Chloroplasts, the green differentiation form of plastids, are the sites of photosynthesis and other important plant functions. Genetic and genomic technologies have greatly boosted the rate of discovery and functional character- ization of chloroplast proteins during the past decade. Indeed, data obtained using high-throughput methodologies, in particular proteomics and transcriptomics, are now routinely used to assign functions to chloroplast proteins. Our knowl- edge of many chloroplast processes, notably photosynthesis and photorespiration, has reached such an advanced state that biotechnological approaches to crop improvement now seem feasible. meanwhile, efforts to identify the entire com- plement of chloroplast proteins and their interactions are progressing rapidly, making the organelle a prime target for systems biology research in plants.

关键词： Chloroplast biology genetics genomics molecular biology proteomics transcriptome analysis

一种改进的地震灾害倒塌房屋遥感信息自动识别方法

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科学通报 2009年第17期54卷 2581-2585页

作者：郭华东鹿琳琳马建文 martino pesaresi 苑方艳中国科学院对地观测与数字地球科学中心数字地球科学重点实验室北京100190 中国科学院研究生院北京100049 Institute for the Protection and Security of the Citizen (IPSC) European Commission Joint Research Center

地震灾害倒塌房屋的识别是地震灾害损失评价的重要环节,也是判断地震强度的指标之一.5.12汶川大地震发生后,中欧地震灾害评估项目组使用自动识别技术处理0.5m分辨率的ADS40航空遥感数据,需要克服航空遥感数据中未倒塌混凝土桥梁、滑坡... 详细信息

地震灾害倒塌房屋的识别是地震灾害损失评价的重要环节,也是判断地震强度的指标之一.5.12汶川大地震发生后,中欧地震灾害评估项目组使用自动识别技术处理0.5m分辨率的ADS40航空遥感数据,需要克服航空遥感数据中未倒塌混凝土桥梁、滑坡体滚石与倒塌房屋反射光谱的相似性.经过多次实验改进后的技术,一次性识别的倒塌房屋面积覆盖率优于95%.介绍了这次实验过程和结果,实验表明,该方法也可以用于其他灾害造成的倒塌房屋的自动识别.

关键词：地震灾害航空遥感影像倒塌房屋反射光谱相似性自动识别方法

mutants, Overexpressors, and Interactors of Arabidopsis Plastocyanin Isoforms： Revised Roles of Plastocyanin in Photosynthetic Electron Flow and Thylakoid Redox State

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molecular Plant 2009年第2期2卷 236-248页

作者： Paolo pesaresi michael Scharfenberg martin Weigel Irene Granlund Wolfgang R Schroder Giovanni Finazzi Fabrice Rappaport Simona masiero Antonella Furini Peter Jahns Dario Leister Dipartimento di Produzione Vegetale Universita degli studi di Milano c/o Parco Tecnologico Padano Via Einstein Loc Cascina Codazza 1-26900 Lodi Italy Lehrstuhl fur Botanik Department Biologie I Ludwig-Maximilians-Universitat Munchen GroBhaderner Str. 2 D-82152 Planegg-Martinsried Germany Abteilung fhr Pflanzenzuchtung und Genetik Max-Planck-lnstitut fur Zhchtungsforschung Carl-von-Linne-Weg 10 D-50829 Koln Germany Institute of Chemistry and Umea Plant Science Centre (UPSC) Umea University SE-901 87 Umea Sweden Institut de Biologie Physico-Chimique UMR 7141 CNRS-Universite R et M. Curie 13 rue Pierre et Marie Curie F-75005 Paris France Dipatimento di Biologia Universita degli studi di Milano Via Celoria 26 L-20133 Milano Italy Dipartimento Scientifico e Tecnologico Universita degli studi di Verona Strade le Grazie 15 1-37134 Verona Italy Institut fur Biochemie der Pflanzen Heinrich-Heine-Universitat Dusseldorf Universitatsstr. 1 D-40225 Dusseldorf Germany

Two homologous plastocyanin isoforms are encoded by the genes PETE1 and PETE2 in the nuclear genome of Arabidopsis thaliana. The PETE2 transcript is expressed at considerably higher levels and the PETE2 protein is the more abundant isoform. Null mutations in the PETE genes resulted in plants, designated pete1 and pete2, with decreased plastocyanin contents. However, despite reducing plastocyanin levels by over -90%, a pete2 null mutation on its own affects rates of photosynthesis and growth only slightly, whereas pete1 knockout plants, with about 60-80% of the wild-type plastocyanin level, did not show any alteration. Hence, plastocyanin concentration is not limiting for photosynthetic elec- tron flow under optimal growth conditions, perhaps implying other possible physiological roles for the protein. Indeed, plastocyanin has been proposed previously to cooperate with cytochrome C6A （Cyt C6A） in thylakoid redox reactions, but we find no evidence for a physical interaction between the two proteins, using interaction assays in yeast. We observed homodimerization of Cyt C6A in yeast interaction assays, but also Cyt C6A homodimers failed to interact with plastocyanin. moreover, phenotypic analysis of atc6-1 pete1 and atc6-1 pete2 double mutants, each lacking Cyt C6A and one of the two piastocyanin-encoding genes, failed to reveal any genetic interaction. Overexpression of either PETE1 or PETE2 in the pete1 pete2 double knockout mutant background results in essentially wild-type photosynthetic performance, excluding the possibility that the two plastocyanin isoforms could have distinct functions in thylakoid electron flow.

关键词：蛋白质亚型基因拟南芥属氧化还原一氧化氮胰岛素植物质体蓝素

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7T脑mR检查的短期不良反应:单中心经验

国际医学放射学杂志 2014年第5期 506-506页

作者： m.Cosottini D.Frosini L.Biagi I.pesaresi m.Costagli G.Tiberi 张其锐 Department of Translational Research and New Technologies in medicine and Surgery University of Pisa

摘要目的研究超高场强(7 T)mR设备行脑成像时被试的耐受性。方法采用超高场强(7 T)mR设备检查了180例被试。被试者耐受性试验分成两部分(在检查床移动过程中

关键词： mR 耐受性被试不良反应场强