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Human Seroreactivity to Secreted molecules of Corynebacterium pseudotuberculosis

Advances in microbiology 2022年第3期12卷 150-158页

作者： Ramon mendes dos Santos Silvânia maria Andrade Cerqueira Caio Lopez Borge Andrade Gabriel Saldanha müller Vitória Costa de menezes Santos Hévlyn Ribeiro de Araújo Samanta Queiroz Rogério Reis Conceição Luiz Gustavo Freitas Oliveira marcos Borges Ribeiro Silvana marchioro Lilia Ferreira de moura-Costa Fúlvia Soares Campos de Sousa maria da Conceição Aquino de Sá José Tadeu Raynal Rocha Filho Soraya Castro Trindade Eduardo martins Netto Roberto meyer Songelí menezes Freire Laboratory of Immunology and molecular Biology of the Health Sciences Institute of the Federal University of Bahia Bahia Brazil Specialized Hospital Otávio mangabeira Bahia Brazil microbiology of the Federal University of Bahia Bahia Brazil Laboratory of Infectology of the University Hospital Complex Professor Edgard Santos Salvador Bahia Brazil

Corynebacterium pseudotuberculosis is an infectious agent that occurs in small ruminants causing caseous lymphadenitis, and more rarely in humans causing lymphadenitis and pneum.nia. The breeding small ruminants have great economic importance in Brazil. Rural farm workers and veterinary students who acquired this disease suffered from weakening symptoms for weeks, and the identification of the etiological agent was time-consuming and complex. Due to the low prevalence of case records, there is probably no available commercial diagnostic kit for C. pseudotuberculosis infection in humans. This study aimed to describe human seroreactivity to secreted antigens from C. pseudotuberculosis. Reactivity of serum from farm workers (n = 14), individuals who work with the bacillus at laboratory (n = 8) or individuals without contact (n = 25) was tested with secreted proteins from PAT10 strain of C. pseudotuberculosis by Western blotting. Samples of all (100%) farm workers showed reactivity to 31 kDa, 71 kDa and 164 kDa proteins, while laboratory workers showed 87.5%, 62.5 % and 37.5%, and no-contact 20%, 0% and 16%, respectively. All sera recognized the 275 kDa protein. Our data suggest that C. pseudotuberculosis secreted proteins are antigenic in humans and the recognition profiles allowed the identification of individuals with and without prior contact with this bacillus. This is the first paper which describes human reactivity to C. pseudotuberculosis in serum samples of workers in Brazil.

关键词： Human Immunoreactivity Antigenicity Corynebacterium pseudotuberculosis Secreted Proteins Western Blot

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A regionalized IO-model to value seasonal recreational ecosystem services in a mountain National Park in Spain

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Journal of mountain Science 2022年第1期19卷 211-227页

作者： Víctor mARTIN BARROSO mónica de CASTRO-PARDO Pascual FERNÁNDEZ mARTÍNEZ João C.AZEVEDO Department of Applied Economics I and history and economic institutions Faculty of Law and Social SciencesUniversidad Rey Juan CarlosCampus of VicálvaroTulipanMóstoles 28933Spain Department of Financial and Actuarial Economics and Statistics-Statistics and Operational Research Complutense University of MadridCampus of SomosaguasPozuelo de Alarcon 28223Spain Centro de Investigação de montanha Instituto Politécnico de BragançaCampus de Santa ApolóniaBragança 5300-253Portugal

Recreational Ecosystem Services(RES)are among the most frequently evaluated ecosystem *** are seen as a major opportunity for sustainable development in areas of high ecological value resulting from the presence of emblematic species,habitats or scenery,often integrated in conservation areas,in particular in mountain areas affected by depopulation,rising environmental risks and ***,the assessment of RES has been based on Contingent Valuation and Choice Experiments,methods with limitations related to their ***,Input-Output(IO)models provide a very accurate and effective way of valuation of RES with regional information regarding interindustry *** data on nature tourist expenditure may be only available annually,tourism inflows have a strong seasonal behavior,which impacts the profitability of several local economic *** this paper,we firstly constructed a novel regionalized IO-model based on Cross-Industry Location Quotients using employment data and,secondly,based on monte Carlo simulation,we estimated nature tourist expenditure monthly using data on nature tourism *** method allowed for a more precise RES evaluation,estimating direct,indirect and induced monthly economic impacts of nature-based *** method was applied to the Ordesa and monte Perdido(OmP)National Park in the Spanish *** estimate the average tourist expenditure,we collected and analyzed spending on commerce,hospitality and restaurants,leisure,and transport of 385 *** suggest that using a regionalized IO model leads to a considerable reduction of over-estimation of the economic impact of tourist expenditure estimated by conventional *** into consideration the direct,indirect and induced effects of tourist expenditure in 2016-2018,the total annual output effect in the OmP National Park amounted to€208.8 million,while the total income effect amounted to€86.29 ***,a total of

关键词： Input-Output monte Carlo Simulation Location quotients Recreational Ecosystem Services Nature tourism Sustainable mountain development

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Autologous treatment for ALS with implication for broad neuroprotection

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Translational Neurodegeneration 2022年第1期11卷 718-736页

作者： Daehwan Kim Subin Kim Ashley Sung Neetika Patel Nathan Wong michael J.Conboy Irina m.Conboy Department of Bioengineering and QB3 Institute University of CaliforniaBerkeleyCA 94720USA

Background: Amyotrophic lateral sclerosis (ALS) is characterized by a progressive loss of motor neurons (mNs), leading to paralysis, respiratory failure and death within 2-5 years of diagnosis. The exact mechanisms of sporadic ALS, which comprises 90% of all cases, remain unknown. In familial ALS, mutations in superoxide dismutase (SOD1) cause 10% of cases. methods: ALS patient-derived human-induced pluripotent stem cells (ALS hiPSCs, harboring the SOD1AV4 mutation), were differentiated to mNs (ALS-mNs). The neuroprotective effects of conditioned medium (Cm) of hESCs (H9), wt hiPSCs (WTC-11) and the ALS iPSCs, on mN apoptosis and viability, formation and maintenance of neurites, mitochon-drial activity and expression of inflammatory genes, were examined. For in vivo studies, 200 μl of Cm from the ALS iPSCs (CS07 and CS053) was injected subcutaneously into the ALS model mice (transgenic for the human SOD1G93A mutation). Animal agility and strength, muscle innervation and mass, neurological score, onset of paralysis and lifes-pan of the ALS mice were assayed. After observing significant disease-modifying effects, the Cm was characterized biochemically by fractionation, comparative proteomics, and epigenetic screens for the dependence on pluripotency. Cm of fibroblasts that were differentiated from the wt hiPSCs lacked any neuroprotective activity and was used as a negative control throughout the studies. Results: The secretome of PSCs including the ALS patient iPSCs was neuroprotective in the H2O2 model. In the model with pathogenic SOD1 mutation, ALS iPSC-Cm attenuated all examined hallmarks of ALS pathology, rescued human ALS-mNs from denervation and death, restored mitochondrial health, and reduced the expression of inflam-matory genes. The ALS iPSC-Cm also improved neuro-muscular health and function, and delayed paralysis and mor-bidity in ALS mice. Compared side by side, cyclosporine (CsA), a mitochondrial membrane blocker that prevents the leakage of mitochondri

关键词： Neurodegeneration Amyotrophic lateral sclerosis SOD1 mutation Pluripotent stem cells Secretome

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Use of Silicon in mitigating Ammonium Toxicity in maize Plants

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American Journal of Plant Sciences 2015年第11期6卷 1780-1784页

作者： Cid Naudi Silva Campos Renato de mello Prado Cassiano Garcia Roque Antonio Joao de Lima Neto Luiz Junior Pereira marques Aridênia Peixoto Chaves Claudia Amaral Cruz Department of Soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil Department of Soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil Universidade Federal de mato Grosso do Sul Chapad&atildeo do Sul Brazil Universidade Federal de Viçosa Vi&ccedilosa Brazil Department of Applied Biology and Agriculture Universidade Estadual Paulista/FCAV Jaboticabal Brazil Department of Plant Science Universidade Federal Rural do Semi-árido Mossoró Brazil m.Sc. in Agronomy Natal Brazil

Silicon is a beneficial element that can mitigate abiotic stresses, such as ammonium toxicity. The objective herein was to evaluate the effects of silicon (Si) on mitigating toxicity caused by excess ammonium in maize plants grown in nutrient solution. An experiment was conducted with maize plants (cultivar DKB 390 VT Pro II) grown in a greenhouse in pots (8 L) in a hydroponic system. The treatments were arranged in a 2 × 2 factorial, consisting of two ammonium concentrations (30 and 60 mmol·L-1) in the absence and presence of Si (10 mmol·L-1), arranged in a completely randomized design with six repetitions. At 28 days after applying the treatments the dry mass of shoots and roots was evaluated, along with accumulation of silicon and nitrogen in the shoots. The use of silicon resulted in increases in the studied variables, regardless of ammonium concentrations. Silicon reduced the effect of toxicity caused by ammonium excess in maize plants, resulting in greater growth and dry matter accumulation.

关键词： Zea mays L. Beneficial Element Nitrogen Nutritional Disorder Abiotic Stress

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Dengue in renal transplant recipients: Clinical course and impact on renal function

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World Journal of Transplantation 2017年第1期7卷 57-63页

作者： Paula Frassinetti Castelo Branco Camur?a Fernandes Reed AndréSiqueira Evelyne Santana Gir?o Rainne AndréSiqueira márcia Uchoa mota Leyla Castelo Branco Fernandes marques Silvana Cristina Albuquerque Andrade Wilson mendes Barroso S?nia Leite Silva Bruno Gomes Rodrigues dos Santos Claúdia maria Costa de Oliveira Kidney Transplant Unit Hospital Universitário Walter Cantídio Universidade Federal do Ceará Department of Nephrology of Universidade Estadual do Ceará Universidade Estadual do Ceará

AIm To present clinical characteristics from renal transplant recipients with dengue fever and its impact on graft *** We retrospectively evaluated 11 renal transplant recipients(RTR) with dengue infection confirmed by laboratory test, between January 2007 and July 2012, transplanted in the Renal Transplant Center of Walter Cantídio University Hospital from Federal University of Ceará. RESULTS Positive dengue serology(Igm) was found in all patients. The mean time between transplant and dengue infection was 43 mo. Fever was presented in all patients. Nine patients presented with classical dengue and two(18%) with dengue hemorrhagic fever. All cases had satisfactory evolution with complete recovery of the symptoms. The time for symptom resolution varied from 2 to 20 d, with an average of 9 d. An increase of creatinine after the infection was observed in three(27.2%) patients with no clinically impact on the kidney graft function. CONCLUSION RTR with dengue infection seems to have a clinical presentation and evolution similar to those seen in the general population, with no long-term damage to patient and to the graft.

关键词： Kidney Renal Transplant Dengue Clinical Brazil

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Biotechnological Potential of Carotenoids Produced by Extremophilic microorganisms and Application Prospects for the Cosmetics Industry

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Advances in microbiology 2020年第8期10卷 397-410页

作者： Tayane de Cássia Dias mendes-Silva Rosileide Fontenele da Silva Andrade marcio Akio Ootani Paulo Vitor Dias mendes Rafael Artur de Queiroz Cavalcanti de Sá milena Roberta Freire da Silva Karolayne Silva Souza maria Tereza dos Santos Correia márcia Vanusa da Silva maria Betânia melo de Oliveira Department of Biochemistry Federal University of Pernambuco Recife Brazil National Program Postdoctoral CAPES (PNPD) Catholic University of Pernambuco Recife Brazil Bioprocess Laboratory Center of Strategic Technologies Northeast Recife Brazil Design Department Federal University of Pernambuco Recife Brazil Nucleus of Bioprospecting and Conservation of the Caatinga Semiarid National Institute/Ministry of Science Technology Innovations and Communications Campina Grande Brazil

Carotenoids are isoprenoid pigments used in food, chemical, textile, pharmaceutical, and cosmetic industries. They act not only as dyes and provitamins A but also have antioxidants, photoprotective, antimicrobial properties, among others. This class of pigment can be obtained traditionally by plants or chemical synthesis, but they have some disadvantages. In recent years, search for alternative sources has been an important strategy for the carotenoid industries. microbial synthesis is an alternative that has shown good yields, speed, and reduced production costs. Hostile environments, such as the Caatinga domain, represent an interesting source of microorganisms that produce biomolecules, especially carotenoids, because of oxidative stress caused by sunlight. Thus, this region has been attracting the attention of the scientific community and industry for the use of these organisms in the production of carotenoids and applications in cosmetic products;since these compounds have interesting antioxidant and photoprotective properties. In this review, general characteristics of carotenoids, sources of production, industrial applicability, and commercialization will be discussed, as well as perspectives on the production of carotenoids from microorganisms isolated from the Caatinga and their application in anti-UV products.

关键词： Natural Pigment microbial Production Hostile Environments Antioxidant Photoprotection

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Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia(Hemsl) *** leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 245-253页

作者： Bruno Rafael Barboza Bárbara Rafaela da Silva Barros Bárbara de Azevedo Ramos maiara Celine de moura Thiago Henrique Napoleao maria Tereza dos Santos Correia Luana Cassandra Breitenbach Barroso Coelho Iranildo José da Cruz Filho Ana maria Souto maior Túlio Diego da Silva Leylianne de Cássia Rodrigues Nerys Edson Renan Barros de Santana Cláudia Sampaio de Andrade Lima Virgínia maria Barros de Lorena Cristiane moutinho Lagos de melo Department of Antibiotics Biosciences Centre Federal University of Pernambuco Department of Biochemistry Biosciences Centre Federal University of Pernambuco Center for Strategic Techmologies of the Northeast Department of Biophysics Biosciences Centre Federal University of Pernambuco Aggeu magalhaes Institute IAM/FIOCRUZ

Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal ***:The saline extract was prepared,with NaCl(0.15 m),by constant stirring of the dried and pulverized leaves,followed by volume reduction by *** extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was *** antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice ***:Fifteen compounds were identified belonging to two main classes terpenoids and *** extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of ***,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these ***:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.

关键词： Tithonia diversifolia Extract Antioxidant Antifungal properties Immunostimulation

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Are flexibility and muscle-strengthening activities associated with functional limitation?

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Sports medicine and Health Science 2022年第2期4卷 95-100页

作者： Craig E.Pfeifer Leanna m.Ross Samantha R.Weber Xuemei Sui Steven N.Blair Department of Exercise Science University of South Carolina921 Assembly StColumbiaSC29208United States Applied Sport and Exercise Sciences University of GloucestershireUniversity of Gloucestershire Oxstalls CampusOxstalls LnLonglevens GL29HWUnited Kingdom Duke molecular Physiology Institute Duke University School of Medicine300 N Duke StDurhamNC27701United States Department of Nursing and Health Sciences Limestone University1115 College DrGaffneySC29340United States

This retrospective cohort study examined the relationship between self-reported participation in flexibility and muscular strengthening activities and the development of functional limitation(i.e.,once an individual has difficulty with or becomes unable to perform activities of daily living).Data were obtained from 1318 adults(mean age 49.59.7 years;98.7%Caucasian;14.9%female)enrolled in the Aerobics Center Longitudinal Study from 1979 to 2004 and free of functional limitation at ***-back health surveys were used to prospectively determine incident functional *** in muscle-strengthening and flexibility activities was assessed via *** logistic regression analyses were used to determine the odds ratios(OR)and corresponding 95%confidence intervals for developing functional limitation during follow-up based on participation in general and specific categories of flexibility(‘Stretching’,‘Calisthenics’,or‘Exercise Class’)and musclestrengthening activities(‘Calisthenics’,‘Free Weights’,‘Weight Training machines’,or‘Other’).Overall,42.6%of the sample reported incident functional *** adjusting for potential confounders(e.g.,age,sex,cardiometabolic risk factors),those who reported performing muscle-strengthening activities in general(n=685)were at lower risk of developing functional limitation[OR=0.79(0.63–1.00)].In addition,the specific flexibility activities of stretching(n=491)and calisthenics(n=122)were associated with 24%and 38%decreased odds of incident functional limitation,*** muscle-strengthening,stretching,and calisthenics activities are prospectively associated with decreased risk of incident functional limitation in generally healthy,middleaged and older ***,both public health and rehabilitation programs should highlight the importance of flexibility and muscle-strengthening activities during adulthood to help preserve functional capacity.

关键词： Functional capacity Quality of life Aging Exercise Independence Health

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波尔图Kinematix办公室

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现代装饰 2015年第6期 112-115页

作者： Diogo Aguiar Teresa Otto Joo Jesus Tània Costa Coll lvaro Vila Iturbe Dinis Sottomayor 姜敏华 LIKE建筑事务所

项目地址葡萄牙项目面积180m^2竣工时间2014.05设计单位LIKE建筑事务所本案是LIKE建筑事务所的设计团队为一家专业致力于医疗保健技术和健身产品的IT公司Kinematix打造的研发总部。项目坐落于葡萄牙第二大城市——波尔图一栋具有象征意... 详细信息

项目地址葡萄牙项目面积180m^2竣工时间2014.05设计单位LIKE建筑事务所本案是LIKE建筑事务所的设计团队为一家专业致力于医疗保健技术和健身产品的IT公司Kinematix打造的研发总部。项目坐落于葡萄牙第二大城市——波尔图一栋具有象征意义的六十年代历史建筑内,其前身为卢米埃尔电影院。该建筑上层现存的空间安装有排列整齐的窗户,覆盖建筑物的整个立面,极具韵律感。

关键词：波尔建筑事务所 Kinematix 项目地址自然光线设计单位历史建筑韵律感象征意义项目面积

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Chaperone proteins as ameliorators of α-synuclein-induced synaptic pathologies: insights into Parkinson’s disease

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Neural Regeneration Research 2021年第6期16卷 1198-1199页

作者： Susan m.L.Banks Audrey T.medeiros Rui Sousa Eileen m.Lafer Jennifer R.morgan Department of Biology Florida Southern CollegeLakelandFLUSA Neuroscience Graduate Program Brown UniversityProvidenceRIUSA Department of Biochemistry and Structural Biology and Center for Biomedical Neuroscience The University of Texas Health Science Center at San AntonioSan AntonioTXUSA The Eugene Bell Center for Regenerative Biology&Tissue Engineering Marine Biological LaboratoryWoods HoleMAUSA

α-Synuclein accumulation causes synaptic vesicle trafficking defects and may underlie neurodegenerative disorders:Neurodegenerative disorders,such as Parkinson’s disease(PD)and other synucleinopathies,impact the lives of millions of patients and their *** include PD,dementia with Lewy Bodies(DLB),multiple system atrophy,and several Alzheimer’s Disease *** are clinically characterized by intracellular inclusions called Lewy Bodies,which are rich in atypical aggregates of the protein α-*** dopaminergic neurons in the substantia nigra are particularly susceptible toα-synuclein-induced aggregation and neurodegeneration,glutamatergic neurons in other brain regions(***)are also frequently affected in PD and other synucleinopathies(Schulz-Schaeffer 2010).

关键词： cortex Alzheimer insight

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