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serological diagnosis of nasop.aryngeal carcinoma by enzyme linked immunosorbant assay:op.imization,standardization and diagnostic criteria

引用

chinese medical j.urnal 1998年第6期 51-56页

作者： m.h. ng, h.l. chen, r.x. luo, k.h. chan, p.c.y. woo, j.s.t. sham, j. huang, w.h.seto p. smith and b.e. griffin

Ob.e.tives to p.oduce an enzyme linked immunosorbant assay (elIsA) to detect antibodies against ep.tein barr virus (ebV) sp.cified nuclear antigen 1 (ebNA 1) and the 18kD ebV matr.x.p.otein, and to determine and op.imize its sensitivity and sp.cificity for the diagnosis of nasop.aryngeal carcinoma (Np.).methods we used a combination of highly p.rified glutathione transferase fusion p.oteins of the 40kD carboxy domain of ebNA1 and the 18kD ebV matr.x.p.otein for coating elIsA p.ates. In three sep.rate studies, we tested for IgA antibodies in serum sp.cimens from 28 ebV seronegative donors, 284 ebV serop.sitive donors and 160 newly diagnosed Np. p.tients. by comp.ring the sensitivity and sp.cificity of diagnosis obtained for different cutoff values, we derived several quantitative p.rameters to evaluate assay p.rformance, establish ob.e.tive diagnostic criteria which op.imize the intrinsic diagnostic cap.bility of the assay and assess the significance of individual test results, resp.ctively. Op.imum cutoff op.ical density (OD) is defined as the cutoff OD where sensitivity of the assay equals its sp.cificity, and resolution of the assay is indicated by the value of sensitivity (or sp.cificity) determined at the op.imum cutoff OD. Diagnosis of Np. was achieved by setting a cutoff zone at +/-20% of this *** All the ebV seronegative donors tested were not reactive, and most of the ebV serop.sitive donors were weakly reactive, wh.l. the maj.rity of Np. p.tients were moderately or strongly reactive. wh.l. the assay was thus shown to be sp.cific for ebV, there was an overlap.in the level of these serum antibodies between few individuals of the two latter group.. It was shown that the assay p.rformed equally well in two sep.rate studies conducted under different testing conditions and using different collections of sera in that assay resolution determined on these occasions were 86% and 87% resp.ctively. Diagnosis of Np. can be achieved at the same exp.cted sensiti

关键词： serological diagnosis of nasop.aryngeal carcinoma by enzyme linked immunosorbant assay

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