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What have we known so far for fluorescence staining and quantification of microplastics:A tutorial review

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Frontiers of Environmental Science & Engineering 2022年第1期16卷 37-50页

作者： Shengdong Liu Enxiang Shang Jingnan Liu Yining Wang Nanthi bolan mbkirkham Yang Li Key Laboratory of Water and Sediment Sciences of ministry of Education State Key Laboratory of Water Environment SimulationSchool of EnvironmentBeijing Normal UniversityBeijing 100875China College of Science and Technology Hebei Agricultural UniversityHuanghua 061100China School of Agriculture and Environment The University of Western AustraliaPerthWA 6001Australia The UWA Institute of Agriculture The University of Western AustraliaPerthWA 6001Australia Global Innovative Centre for Advanced Nanomaterials College of EngineeringScience and EnvironmentUniversity of NewcastleCallaghanNSW 2308Australia Department of Agronomy Throckmorton Plant Sciences CenterKansas State UniversityManhattanKS 66506USA

Understanding the fate and toxicity of microplastics(mPs,<5 mm plastic particles)is limited by quantification *** paper summarizes the methods in use and presents new ***,sampling and pretreatment processes ofmPs,incl... 详细信息

Understanding the fate and toxicity of microplastics(mPs,mm plastic particles)is limited by quantification *** paper summarizes the methods in use and presents new ***,sampling and pretreatment processes ofmPs,including sample collection,digestion,density separation,and quality control are *** the promising and convenient staining procedures and quantification methods for mPs using fluorescence dyes are *** factors that influence the staining of mPs,including their physicochemical properties,are summarized to provide an optimal operation *** general,the digestion step is crucial to eliminate natural organic matter(NOm)to avoid interference in *** was reported to be the most appropriate solvent,and 10–20μg/mL are recommended as optimal dye *** addition,a heating and cooling procedure is recommended to maintain the fluorescence intensity of mPs for two *** staining,a fluorescence microscope is usually used to characterize the morphology,mass,or number of mPs,but compositional analysis cannot be determined with *** fluorescence staining methods have been implemented to study mP abundance,transport,and toxicity and have been combined with other chemical characterization techniques,such as Fourier transform infrared spectroscopy and Raman *** studies are needed to focus on the synthesis of novel dyes to avoid NOm’s *** need to be combined with other spectroscopic techniques to characterize plastic composition and to develop image-analysis *** stability of stained mPs needs to be improved.

关键词： Plastic particles Fluorescence dyes Identification Concentration quantification

Use of ^(137)Cs and ^(210)Pb_(ex) fallout radionuclides for spatial soil erosion and redistribution assessment on steeply sloping agricultural highlands

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Journal of mountain Science 2021年第11期18卷 2888-2899页

作者： Jung-Hwan YOON Young-Nam KIm Kye-Hoon KIm m.b.kirkham Hyuck Soo KIm Jae E.YANG Kangwon Institute of Inclusive Technology Kangwon National UniversityChuncheon 24341Republic of Korea Department of biological Environment Kangwon National UniversityChuncheon 24341Republic of Korea Institute of Agriculture and Life Science Gyeongsang National UniversityJinju 52828Republic of Korea Department of Environmental Horticulture University of SeoulSeoul 02504Republic of Korea Department of Agronomy Kansas State UniversityManhattanKS 66506-0110USA

The steeply sloping agricultural highlands in Korea have severe soil *** of both soil erosion and sedimentation in these highlands is necessary to make plans for soil-conservation measures,but it is not feasible using existing soil-erosion *** study measured the site-specific concentrations of ^(137)Cs and ^(210)Pb_(ex) on both a highland slope(33%slope)and a reference site(undisturbed flat area)to estimate soil erosion and *** use of the fallout radionuclide(FRN)method was evaluated to see if it is a suitable method for characterizing soil *** were compared with those obtained with the Universal Soil Loss Equation(USLE),which is an empirical model that estimates annual soil *** average concentrations of ^(137)Cs and ^(210)Pb_(ex) at the reference site were 11.57±0.24 bq kg^(-1) and 59.74±4.2 bq kg^(-1),*** of ^(137)Cs and ^(210)Pb_(ex) in the experimental slope were 16.4%and 10.8%,respectively,of those at the reference *** inventories were lower at the upper point of the slope than those at the basal point of the *** of ^(137)Cs and ^(210)Pb_(ex) were significantly correlated with available phosphorus,organic matter,CEC,and exchangeable *** of soil redistribution rate using ^(137)Cs and ^(210)Pb_(ex) showed site-specific variations at different points along the slope,and respective ranges were^(-1)7.46~-207.51 and 1.55~-581.38 mgha^(-1) yr^(-1),which indicated that more erosion was assessed by ^(210)Pb_(ex) than by ^(137)*** analysis showed that soil erosion occurred along the entire slope,except for the bottom point of the slope where 1.55 mgha^(-1) yr^(-1) of sediment *** USLE provided a single value of the average annual soil loss in the entire slope,which was either 166 or 398 mgha^(-1) yr^(-1),depending on the soil erodibility factor(soil series factor and calculated factor from soil sample analysis)used in t

关键词： Soil erosion Steep highland Fallout radionuclide ^(137)Cs ^(210)Pb_(ex) USLE

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钙钠离子对高粱和小麦乙烯释放的影响

河北农业大学学报 1990年第3期13卷 51-54页

作者：张敬贤 m.b.kirkham 河北省农林科学院农业物理生理生化研究所美国堪萨斯州立大学农学系

本文阐述了钙、钠离子对小麦、高粱抗旱性不同的品种叶片与根乙烯释放的影响。钙和钠离子对小麦和高架根的乙烯释放有明显的抑制效应。钠离子胁迫下,小麦根乙烯释放量多于高粱根;钙离子胁迫下则以高粱根乙烯释放量为多。钙或钠离子胁迫... 详细信息

本文阐述了钙、钠离子对小麦、高粱抗旱性不同的品种叶片与根乙烯释放的影响。钙和钠离子对小麦和高架根的乙烯释放有明显的抑制效应。钠离子胁迫下,小麦根乙烯释放量多于高粱根;钙离子胁迫下则以高粱根乙烯释放量为多。钙或钠离子胁迫下,高粱或小麦不抗旱品种根乙烯释放量多于抗早品种。就叶片乙烯释放而言,高粱乙烯释放量少于小麦。两离子对高粱抗旱品种叶片乙烯释有促进作用,对不抗旱品种影响甚微。小麦不抗旱品种叶片乙烯释放为钙钠处理所抑制。胁迫下两种作物抗旱品种叶片的乙烯释放量高于其不抗旱品种的。这些结果表明,植物的乙烯释放对盐分胁迫的反应随组织器官和基因型而异。

关键词：高粱小麦乙烯钙钠

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冬小麦叶片的方向性、光的接受和生长

麦类作物学报 1985年第6期 39-42页

作者： m.b.kirkham ,奚雷译

决定作物生长最重要的因素之一,是植株接收的日光量.冬小麦生长的地区,晚春封行以前的生长季节前期(秋、冬、早春)太阳辐射的截获量特别重要.在比较冷的时期内,叶子吸收辐射量的多少是很重要的,因为辐射不仅为光合作用提供能量,还为生... 详细信息

决定作物生长最重要的因素之一,是植株接收的日光量.冬小麦生长的地区,晚春封行以前的生长季节前期(秋、冬、早春)太阳辐射的截获量特别重要.在比较冷的时期内,叶子吸收辐射量的多少是很重要的,因为辐射不仅为光合作用提供能量,还为生长提供温度.kirkham等1980年报道,用八个冬小麦品种的试验表明,封行前不同品种截获的辐射不同.生育前期截获辐射越多的品种,籽粒产量就越高.

关键词：冬小麦指向封行生长季节叶片冬麦封垄直射辐射强度叶面积系数叶面积指数

乙烯利对抗旱性不同的高粱品种水势和渗透势的影响

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华北农学报 1989年第S1期4卷 103-107页

作者：张敬贤 m.b.kirkham 河北省农林科学院农业物理生理生化研究所美国堪萨斯州立大学

本文研究了乙烯利对高粱抗旱和干旱敏感型品种水势和渗透势的影响.乙烯利处理能显著增加植株叶片的乙烯释放量.在浇水条件下,乙烯利使叶水势、渗透势降低;但在水分胁迫时,乙烯利却使叶水势、渗透势提高.在两种水分状况下,乙烯利处理与... 详细信息

本文研究了乙烯利对高粱抗旱和干旱敏感型品种水势和渗透势的影响.乙烯利处理能显著增加植株叶片的乙烯释放量.在浇水条件下,乙烯利使叶水势、渗透势降低;但在水分胁迫时,乙烯利却使叶水势、渗透势提高.在两种水分状况下,乙烯利处理与不处理植株叶片的气孔阻力相似.不同品种对乙烯利的反应差异随水分处理而异.

关键词：高粱乙烯利气孔阻力水势渗透势水分胁迫

Quantum correlations and entanglement in coupled optomechanical resonators with photon hopping via Gaussian interferometric power analysis

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Chinese Physics b 2024年第5期33卷 204-211页

作者： Y.Lahlou b.maroufi m.Daoud LPHE-mS Department of PhysicsFaculty of SciencesMohammed V UniversityRabatMorocco LPmS Department of PhysicsFaculty of SciencesIbn Tofail UniversityKénitraMorocco

Quantum correlations that surpass entanglement are of great importance in the realms of quantum information processing and quantum ***,for quantum systems prepared in pure states,it is difficult to differentiate between quantum entanglement and quantum ***,this indistinguishability is no longer holds for mixed *** contribute to a better understanding of this differentiation,we have explored a simple model for both generating and measuring these quantum *** study concerns two macroscopic mechanical resonators placed in separate Fabry–Pérot cavities,coupled through the photon hopping *** system offers a comprehensively way to investigate and quantify quantum correlations beyond entanglement between these mechanical *** key ingredient in analyzing quantum correlation in this system is the global covariance *** forms the basis for computing two essential metrics:the logarithmic negativity(E_(N)^(m))and the Gaussian interferometric power(P_(G)^(m)).These metrics provide the tools to measure the degree of quantum entanglement and quantum correlations,*** study reveals that the Gaussian interferometric power(P_(G)^(m))proves to be a more suitable metric for characterizing quantum correlations among the mechanical modes in an optomechanical quantum system,particularly in scenarios featuring resilient photon hopping.

关键词： quantum correlations entanglement Gaussian interferometric power logarithmic negativity optomechanics photon hopping

Semi-analytical investigation of heat transfer in a porous convective radiative moving longitudinal fin exposed to magnetic field in the presence of a shape-dependent trihybrid nanofluid

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Applied mathematics and mechanics(English Edition) 2024年第1期45卷 197-216页

作者： C.G.PAVITHRA b.J.GIREESHA m.L.KEERTHI Department of PG Studies and Research in mathematics Kuvempu UniversityShankaraghatta 577451India Department of mathematics and Statistics M.S.Ramaiah University of Applied SciencesBengaluru 560058India

The thermal examination of a non-integer-ordered mobile fin with a magnetism in the presence of a trihybrid nanofluid(Fe_3O_4-Au-Zn-blood) is carried out. Three types of nanoparticles, each having a different shape, are considered. These shapes include spherical(Fe_3O_4), cylindrical(Au), and platelet(Zn) configurations. The combination approach is utilized to evaluate the physical and thermal characteristics of the trihybrid and hybrid nanofluids, excluding the thermal conductivity and dynamic viscosity. These two properties are inferred by means of the interpolation method based on the volume fraction of nanoparticles. The governing equation is transformed into a dimensionless form, and the Adomian decomposition Sumudu transform method(ADSTm) is adopted to solve the conundrum of a moving fin immersed in a trihybrid nanofluid. The obtained results agree well with those numerical simulation results, indicating that this research is reliable. The influence of diverse factors on the thermal overview for varying noninteger values of γ is analyzed and presented in graphical representations. Furthermore, the fluctuations in the heat transfer concerning the pertinent parameters are studied. The results show that the heat flux in the presence of the combination of spherical, cylindrical, and platelet nanoparticles is higher than that in the presence of the combination of only spherical and cylindrical nanoparticles. The temperature at the fin tip increases by 0.705 759% when the value of the Peclet number increases by 400%, while decreases by 11.825 13% when the value of the Hartman number increases by 400%.

关键词： convection radiation moving longitudinal fin Adomian decomposition Sumudu transform method(ADSTm) trihybrid nanofluid magnetic field

Direct microwave leaching conditions of rare earth elements in fluorescent wastes

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Journal of Rare Earths 2024年第6期42卷 1165-1174,I0007页

作者： A.bilen b.birol m.N.Saridede S.S.Kaplan m.S.Sonmez Department of metallurgical and materials Engineering Yildiz Technical UniversityIstanbulTurkey Department of Chemical Engineering and materials Science Stevens Institute of TechnologyNew JereyUSA Department of metallurgical and materials Engineering Istanbul Technical UniversityIstanbulTurkey

The luminescent phosphor powder in the fluorescent lamp constitutes 2%of the lamp’s *** can be mentioned that fluorescent wastes are a crucial raw material to produce rare earth *** the present study,microwave leaching process was conducted to dissolve rare earth elements yttrium(Y),europium(Eu),and remaining rare earth elements(REEs)present in the phosphor powder of the fluorescent *** the yields were *** the microwave leaching process,the effects of the temperature(80-160℃),acid type(hydrochloric acid(HCl),nitric acid(HNO_(3)),sulphuric acid(H_(2)SO_(4))),acid concentration(0.5-6 mol/L),solid to liquid ratio(0.1:10-0.5:10)and reaction time(5-90 min)parameters on leaching efficiencies of varying rare earth elements and calcium were *** highest yield was obtained in the direct microwave leaching of fluorescent waste with the experimental conditions of 6 mol/L HCl,160℃,0.1:10 solid-to-liquid ratio(S:L),and 90 *** energy calculations were made,and kinetic models of the reactions were obtained,and it is observed that Y and Eu dissolution is diffusion-controlled,on the other hand,lanthanum(La),cerium(Ce),and terbium(Tb)were examined to be chemical reaction ***,calcium(Ca)and gadolinium(Gd)seem coherent with the mixed ***,mathematical models of all experimental studies are created with the response surface box-behnken method and the correlation coefficients of all the models are over 90%.

关键词： Rare earth elements(REEs) Direct microwave leaching Fluorescent waste Kinetic models Response surface method

RPLP0/TbP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

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World Journal of Stem Cells 2024年第6期16卷 656-669页

作者： Daniel b Ferreira Leticia m Gasparoni Cristiane F bronzeri Katiucia b S Paiva Laboratory of Extracellular matrix biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

bACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliable way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18S rRNA,andβ-actin]without paying attention to whether they are suitable for such experimental conditions or the reason for choosing such ***,such studies use only one gene when minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credibility of these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of gene expression drives the best experimental or therapeutical *** cultivated DPSCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,both for 35 *** evaluated the gene expression of 10 candidates for RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TbP),GAPDH,actin beta(ACTb),tubulin(TUb),aminolevulinic acid synthase 1(ALAS1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,subunit A,flavoprotein(SDHA),and beta-2-micro-globulin(b2m)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analysed by the four main algorithms,ΔCt method,geNorm,NormFinder,and bestKeeper and ranked by the RefFinder *** subdivided the samples into eight *** All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder *** final ranking showed RPLP0/TbP as the two most stable RGs and TUb/b2m as the two least stable *** theΔCt method or NormFinder analysis showed TbP/RPLP0 as the two most stable ***,geNorm analysis showed RPLP0/EF1αin the first *** algorithms’two least stab

关键词： Dental pulp stem cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentiation RefFinder