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油桐尺蠖单粒包埋核型多角体病毒(BusuNPV)BamHI-H片段的序列分析

中国病毒学 1999年第4期14卷 333-342页

作者：罗保君王华林陈新文孙修炼王汉中彭辉银胡志红 Basilm.arif Justm.Valk 中国科学院武汉病毒研究所中荷无脊椎动物病毒学联合实验室武汉430071 LaboratopryformolecularVirology.GreatLakesForestResearchCentre SaultSte.Marieon.P6A5M7.Canada DepartmentofVirology WageningenAgriculturalUniversityBinnenhaven116709PDWageningentheNetherlands

对油桐尺蠖单粒包埋核型多用体病毒（Buzurasuppressariasingle－nucleocapsidnucleopolyhedrovirus，BusuNPV）基因组中BamHI－H片段的序列进行分析，该片段全长2422bp，包括三个开放阅读框：p47基因（AcmNPVORF40的同源区）的5′端，完整的组织蛋白酶基因（cathepsin）（AcmNPVORF127的同源区）和p74基因（AcmNPVORF138的同源区）的3′端。序列比较分析表明，BusuNPV的这三个基因与其它杆状病毒的同源基因具有相同的结构保守区。BusuNPV基因组BamHI－H片段上这三个基因的排列顺序完全不同于AcmNPV相应基因的排列顺序。

关键词： BusuNPV BamHI-H片段基因序列分析

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Bleeding risk with clopidogrel and percutaneous endoscopic gastrostomy

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World Journal of Gastrointestinal Endoscopy 2016年第16期8卷 553-557页

作者： Umair Sohail Chela Harleen Amin O mahdi murtaza arif Douglas L Nguyen matthew L Bechtold Division of Gastroenterology and Hepatology Department of Medicine University of Missouri Department of medicine University of California

AIm To compare bleeding within 48 h in patients undergoing percutaneous endoscopic gastrostomy(PEG) with or without *** After institutional review board approval, a retrospective study involving a single center was conducted on adult patients having PEG(1/08-1/14). Patients were divided into two groups: Clopidogrel group consisting of those patients taking clopidogrel within 5 d of PEG and the non-clopidogrel group including those patients not taking clopidogrel within 5 d of the *** Three hundred and nineteen PEG patients were found. One hundred and sixty-eight males and 151 females with mean body mass index 28.47 ± 9.75 kg/m2 and mean age 65.03 ± 16.11 years were identified. Thirtythree patients were on clopidogrel prior to PEG with 286 patients not on clopidogrel. No patients in either group developed hematochezia, melena, or hematemesiswithin 48 h of percutaneous endoscopic gastrostomy(PEG). No statistical differences were observed between the two groups with 48 h for hemoglobin decrease of > 2 g/dL(2 vs 5 patients; P = 0.16), blood transfusions(2 vs 7 patients; P = 0.24), and repeat endoscopy for possible gastrointestinal bleeding(no patients in either group). CONCLUSION Based on the results, no significant post-procedure bleeding was observed in patients undergoing PEG with recent use of clopidogrel.

关键词： Percutaneous endoscopic gastrostomy Clopidogrel Bleeding Complications Antiplatelets

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Association of mineral and bone biomarkers with adverse cardiovascular outcomes and mortality in the German Chronic Kidney Disease(GCKD)cohort

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Bone Research 2023年第4期11卷 764-771页

作者： Katharina Charlotte Reimer Jennifer Nadal Heike meiselbach matthias Schmid Ulla T.Schultheiss Fruzsina Kotsis Helena Stockmann Nele Friedrich matthias Nauck Vera Krane Kai-Uwe Eckardt markus P.Schneider Rafael Kramann Jürgen Floege Turgay Saritas Department of Nephrology Rheumatologyand Clinical ImmunologyUniversity Hospital RWTH AachenAachenGermany Institute of Experimental medicine and Systems Biology RWTH Aachen UniversityAachenGermany Institute for Cell and Tumor Biology RWTH Aachen UniversityAachenGermany Institute of medical Biometry Informatics and EpidemiologyUniversity Hospital of BonnBonnGermany Department of Nephrology and Hypertension University of Erlangen-NürnbergErlangenGermany Institute of Genetic Epidemiology Faculty of Medicine and Medical Center-University of FreiburgFreiburgGermany Department of medicine IV-Nephrology and Primary Care Faculty of Medicine and Medical Center-University of FreiburgFreiburgGermany Department of Nephrology and medical Intensive Care Charité-Universitätsmedizin BerlinBerlinGermany Department of Nephrology University Medical Center RegensburgRegensburgGermany Institute of Clinical Chemistry and Laboratory medicine University Medicine GreifswaldGreifswaldGermany DZHK(German Centre for Cardiovascular Research) Partner Site GreifswaldUniversity MedicineGreifswaldGermany Department of medicine I Division of NephrologyUniversity Hospital WürzburgWürzburgGermany

mineral and bone disorder(mBD)in chronic kidney disease(CKD)is tightly linked to cardiovascular disease(CVD).In this study,we aimed to compare the prognostic value of nine mBD biomarkers to determine those associated best with adverse cardiovascular(CV)outcomes and *** 5217 participants of the German CKD(GCKD)study enrolled with an estimated glomerular filtration rate(eG FR)between 30–60 mL·min-1 per 1.73 m2 or overt proteinuria,serum osteoprotegerin(OPG),C-terminal fibroblast growth factor-23(FGF23),intact parathyroid hormone(iP TH),bone alkaline phosphatase(BAP),cross-linked C-telopeptide of type 1 collagen(CTX1),procollagen 1intact N-terminal propeptide(P1NP),phosphate,calcium,and 25-OH vitamin D were measured at *** with missing values among these parameters(n=971)were excluded,leaving a total of 4246 participants for *** a median follow-up of6.5 years,387 non-CV deaths,173 CV deaths,645 nonfatal major adverse CV events(mACEs)and 368 hospitalizations for congestive heart failure(CHF)were *** and FGF23 were associated with all outcomes,with the highest hazard ratios(HRs)for *** the final Cox regression model,adjusted for CV risk factors,including kidney function and all other investigated biomarkers,each standard deviation increase in OPG was associated with non-CV death(HR 1.76,95%CI:1.35–2.30),CV death(HR 2.18,95%CI:1.50–3.16),mACE(HR 1.38,95%CI:1.12–1.71)and hospitalization for CHF(HR 2.05,95%CI:1.56–2.69).Out of the nine biomarkers examined,stratification based on serum OPG best identified the CKD patients who were at the highest risk for any adverse CV outcome and mortality.

关键词： Kidney cardiovascular vitamin

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