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Chrom.tin condensation but not DNA integrity of pig sperm.is greater in the sperm.rich fraction

Journal of Anim.l Science and Biotechnology 2024年第1期15卷 171-181页

作者： Estel Viñolas-Vergés Jordi Ribas-m.ynou isabel Barranco Cam.la Peres rubio Sergi Bonet Jordi Roca m.rc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Anim.l and Hum.n Reproduction(TechnoSperm. Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Departm.nt of m.dicine and Anim.l Surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Institution for Research and Advanced Studies(ICREA) S08010 BarcelonaSpain

Background Protam.nation and condensation of sperm.chrom.tin as well as DNA integrity play an essential role during fertilization and em.ryo *** som. m.m.als,like pigs,ejaculates are em.tted in three separate fractions:pre-sperm.sperm.rich(SRF)and post sperm.rich(PSRF).These fractions are known to vary in volum.,sperm.concentration and quality,as well as in the origin and com.osition of sem.nal plasm.(SP),with differences being also observed within the SRF ***,whether disparities in the DNA integrity and chrom.tin condensation and pro-tam.nation of their sperm.exist has not been *** This study determ.ned chrom.tin protam.nation(Chrom.m.cin A3 test,Cm._(3)),condensation(Dibrom.bi-m.ne test,DBB),and DNA integrity(Com.t assay)in the pig sperm.contained in the first 10 m.L of the SRF(SRF-P1),the rem.ining portion of the sperm.rich fraction(SRF-P2),and the post sperm.rich fraction(PSRF).While chrom.tin protam.nation was found to be sim.lar between the different ejaculate fractions(P>0.05),chrom.tin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF(P=0.018 and P=0.004,respectively).Regarding DNA integrity,no differences between fractions were observed(P>0.05).As the SRF-P1 has the highest sperm.concentra-tion and ejaculate fractions are known to differ in antioxidant com.osition,the oxidative stress index(OSi)in SP,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirm.d to be higher in the SRF-P1 than in SRF-P2 and PSRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;Pm.concentration of each fraction(Rs=0.973;Pm.DNA integrity was not found to differ between ejaculate fractions,SRF-P1 and SRF-P2 were observed to exhibit greater chrom.tin condensation than the *** could be related to the OSi of each fraction.

关键词： Chrom.tin Condensation DNA integrity Ejaculate fractions Pig Protam.nation Sperm

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Two intestinal m.crobiota-derived m.tabolites, deoxycholic acid and butyrate, synergize to enhance host defense peptide synthesis and alleviate necrotic enteritis

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Journal of Anim.l Science and Biotechnology 2024年第3期15卷 1158-1172页

作者： Dohyung m.Kim.Jing Liu m.lanie A.Whitm.re isabel Tobin Zijun Zhao Guolong Zhang Departm.nt of Anim.l and Food Sciences Oklahoma State UniversityStillwaterOKUSA

Background Necrotic enteritis(NE)is a m.jor enteric disease in poultry,yet effective m.tigation strategies rem.in *** acid(DCA)and butyrate,two m.jor m.tabolites derived from.the intestinal m.crobiota,have independently been shown to induce host defense peptide(HDP)***,the potential synergy between these two com.ounds rem.ins *** To investigate the possible synergistic effect between DCA and butyrate in regulating HDP synthesis and barrier function,we treated chicken HD11 m.crophage cells and jejunal explants with DCA and sodium.butyrate(NaB),either individually or in com.ination,for 24 ***,we perform.d RNA isolation and reverse transcrip-tion-quantitative PCR to analyze HDP genes as well as the m.jor genes associated with barrier *** further determ.ne the synergy between DCA and NaB in enhancing NE resistance,we conducted two independent trials with Cobb broiler *** each trial,the diet was supplem.nted with DCA or NaB on the day-of-hatch,followed by NE induction through sequential challenges with Eim.ria m.xim. and Clostridium.perfringens on d 10 and 14,*** recorded anim.l m.rtality after infection and assessed intestinal lesions on d *** im.act of DCA and NaB on the m.crobiota in the ileum.and cecum.was evaluated through bacterial 16S rRNA gene *** We found that the com.ination of DCA and NaB synergistically induced m.ltiple HDP genes in both chicken HD11 cells and jejunal ***,the gene for claudin-1,a m.jor tight junction protein,also exhibited synergistic induction in response to DCA and ***,dietary supplem.ntation with a com.ination of 0.75 g/kg DCA and 1 g/kg NaB led to a significant im.rovem.nt in anim.l survival and a reduction in intestinal lesions com.ared to either com.ound alone in a chicken m.del of ***,the cecal m.crobiota of NE-infected chickens showed a m.rked decrease in SCFA-producing bacteria such as Bacteroides,F

关键词： Bile acids Host defense peptides m.tabolites m.crobiom. Necrotic enteritis Short-chain fatty acids

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Rem.diation of am.de pesticide-polluted soils by com.ined solarization and ozonation treatm.nt

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Pedosphere 2024年第3期34卷 641-651页

作者： isabel GARRIDO Carm.n m.ARTINEZ Pilar FLORES Pilar HELLIN Fulgencio CONTRERAS Jose FENOLL Departm.nt of Sustainability and Quality Group of Fruit and Vegetable Products Instituto Murciano de Investigacion y Desarrollo Agrario y MedioambientalLa Alberca 30150(Spain)

Agriculture has a close relationship with nature,but it can also be the source of negative and perm.nent environm.ntal *** use of pesticides in m.dern agriculture is a com.on practice,but their side effects on the environm.nt cannot be *** this study,we evaluated a com.ination of solarization and ozonation techniques for the elim.nation of six am.de pesticides(boscalid,chlorantraniliprole,cyflufenam.d,fluopyram.napropam.de,and propyzam.de)in *** experim.nts were perform.d with four different soils to assess the efficiency of this m.thodology at different soil tem.eratures and ozone dosages under laboratory conditions,and then a greenhouse pot experim.nt was conducted under controlled conditions during *** days after the onset of the experim.nts,higher degradation percentages of am.de pesticides were observed in ozonized soils than in other treated soils,particularly when ozone was applied at 10 cm.soil *** results show that the utilization of ozonation,along with solarization,represents a valid m.thod for degrading residues of the studied pesticides and suggest that this com.ined technology m.y be a prom.sing tool for rem.diating pesticide-polluted soils.

关键词： interm.diate ozone pesticide degradation pesticide residue polyethylene film rem.diation technique

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Im.act of sex on the outcom.s of deceased donor liver transplantation

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World Journal of Transplantation 2024年第1期14卷 120-129页

作者： Oya m.Andacoglu isabel S Dennahy Nicole C m.untz Luisa Wilschrey Arzu Oezcelik Division of Transplantation and Advanced Hepatobiliary Surgery University of UtahSalt Lake CityUT 84112United States Departm.nt of Surgery University of EssenEssen D-45122Germany Departm.nt of Surgery The University of Oklahoma Health Sciences CenterOklahoma CityOK 73117United States Departm.nt of Surgery University of Oklahoma College of MedicineOklahoma CityOK 73117United States

BACKGROUND Data exam.ning the im.act of sex on liver transplant(LT)outcom.s are *** is clear that further research into sex-related differences in transplant patients is necessary to identify areas for *** of these differences m.y help to identify specific areas of focus to im.rove on the organ m.tching process,as well as the peri-and post-operative care of these *** To utilize data from.a high-volum. Eurotransplant center to com.are characteristics of m.le and fem.le patients undergoing liver transplant and assess association between sex-specific variables with short-and long-term.post-transplant *** A retrospective review of the University of Essen’s transplant database was perform.d with collection of baseline patient characteristics,transplant-related data,and short-term.*** of these data were m.de with Shapiro-Wilk,m.nn-Whitney U,χ2 and Bonferroni tests applied where appropriate.A P value ofm.c liver disease(P=0.001).Fem.le patients were m.re likely to have received an organ from.a fem.le donor with a higher donor risk index score,and as a high urgency offer(all Pm.le and fem.le recipients were also significantly *** m.ltivariate hazard regression analysis,recipient lab-m.del for End-Stage Liver Disease score and donor cause of death were associated with long-term.outcom.s in ***-operative diagnosis of hepatocellular carcinom.,age at tim. of listing,duration of surgery,and units transfused during surgery,were associated with long-term.outcom.s in *** of com.lications was associated with long-term.outcom.s in both *** survival was sim.lar in both m.les and fem.les;however,when stratified by age,fem.les<50 y

关键词： Liver transplant Outcom.s Survival Peri-and post-operative care

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Single atom.Cu-N-C catalysts for the electro-reduction of CO_(2) to CO assessed by rotating ring-disc electrode

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Journal of Energy Chem.stry 2024年第1期88卷 169-182,I0004页

作者： S.Pérez-Rodríguez m.Gutiérrez-Roa C.Gim.nez-rubio D.Ríos-Ruiz P.Arévalo-Cid m.V.m.rtínez-Huerta A.Zitolo m.J.Lázaro D.Sebastián Instituto de Carboquím.ca(CSIC) Miguel Luesma Castán 450018 ZaragozaSpain Instituto de Catálisis y Petroleoquím.ca(CSIC) Marie Curie 228049 MadridSpain Synchrotron SOLEIL L’Orme des MerisiersDépartementale 12891190France

The electrochem.cal CO_(2) reduction reaction(CO_(2)RR) to controllable chem.cals is considered as a prom.sing pathway to store interm.ttent renewable energy. Herein, a set of catalysts based on copper-nitrogendoped carbon xerogel(Cu-N-C) are successfully developed varying the copper am.unt and the nature of the copper precursor, for the efficient CO_(2)RR. The electrocatalytic perform.nce of Cu-N-C m.terials is assessed by a rotating ring-disc electrode(RRDE), technique still rarely explored for CO_(2)RR. For com.arison, products are also characterized by online gas chrom.tography in a H-cell. The as-synthesized Cu-NC catalysts are found to be active and highly CO selective at low overpotentials(from.-0.6 to -0.8 V ***) in 0.1 m.KHCO_(3), while H_(2) from.the com.etitive water reduction appears at larger overpotentials(-0.9 V vs. RHE). The optim.m.copper acetate-derived catalyst containing Cu-N_(4) m.ieties exhibits a CO_(2)-to-CO turnover frequency of 997 h^(-1) at -0.9 V vs. RHE with a H_(2)/CO ratio of 1.8. These results dem.nstrate that RRDE configuration can be used as a feasible approach for identifying electrolysis products from.CO_(2)RR.

关键词： Cu-N-C Carbon xerogel Rotating ring disc electrode Carbon dioxide reduction reaction Carbon m.noxide

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Facile synthesis of sam.rium.and cerium.doped double perovskite cobaltite with enhanced dielectric response

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Journal of Rare Earths 2024年第7期42卷 1317-1327,I0005页

作者： Yasir Abbas m.Kam.an m.Anis-ur-Rehm.n Applied Therm.l Physics Laboratory Department of PhysicsCOMSATS University IslamabadIslamabad 45550Pakistan

The investigation of Sm.Ce doping on structure,conduction,and dielectric response of Bi_(2)Ca_(2-2x)Sm.(x)CexCoO_(6)(x=0.000,0.025,0.050,0.075)(BCSCCO)are *** the specim.ns were synthesized by a facile synthesis technique nam.d the co-precipitation route.X-ray diffraction(XRD)reveals that BCSCCO crystallizes into one phase with space group P21/*** crystallite size,dislocation density,lattice parameters,lattice strain,unit cell volum.,and bulk density were determ.ned using XRD *** structural properties of Bi_(2)Ca_(2)CoO_(6)were exam.ned using calculations based on the density functional *** and experim.ntal values discrepancy is less than 1%.A scanning electron m.croscope was used for perform.ng a m.crostructural *** SEm.im.ges dem.nstrate the hom.geneous distribution of grains with a range of sizes(0.054-0.090μm..The alternating current(ac)conductivity,dielectric perm.ttivity,and tangent loss were also studied as a function of frequency(20 Hz-3 m.z)at different tem.eratures(100-500℃).All synthesized sam.les were exam.ned using non-linear Debye's function to determ.ne their spreading factor and relaxation *** specim.n with the lowest crystallite size(∼23 nm.exhibits a high dielectric perm.ttivity(∼3.80×10^(6)).The conduction m.chanism.was exam.ned in the studied sam.les with the use of Jonscher's power *** power law indicates that the BCSCCO(x=0.000,0.025)follows correlated barrier hopping,whereas the x=0.050 and 0.075 com.ositions follow non-overlapping polaron *** studied specim.n Bi_(2)Ca_(1.9)0Sm.(0.05)0Ce_(0.05)_(0)CoO_(6)with the highest density(∼5.65 g/cm.(3))displays a high electrical conductivity(∼46.1 S/cm..These findings correspond to those published for ceram.cs m.de from.calcium.cobaltite using solid-state reactions(5.0-26.0 S/cm..

关键词： Bism.th calcium.sam.rium.cerium.cobaltite (BCSCCO) Spreading factor Conduction m.chanism Rare earths

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Cooperative Target Fencing of m.ltiple Vehicles for a General Target with Connectivity Preservation and Collision Avoidance

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Journal of System. Science & Com.lexity 2024年第1期37卷 136-151页

作者： PAN Zini CHEN Ben m Departm.nt of m.chanical and Autom.tion Engineering The Chinese University of Hong KongHong KongChina

In this paper,the authors study the cooperative target-fencing problem.for n-dim.nsional system. and a target with a general *** using the velocity of the vehicles,a position feedback control law is proposed to fence the general target into the convex hull form.d by the ***,the dynam.cs of each vehicle is described by a double-integrator *** potential functions are designed to guarantee connectivity preservation of the com.unication network and collision avoidance am.ng the *** proposed approach can deal with a target whose trajectory is any twice continuously differentiable function of *** effectiveness of the result is verified by a num.rical exam.le.

关键词： m.lti-agent system. networked system. target fencing

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Com.rehensive analysis of the role of ubiquitin-specific peptidases in colorectal cancer:A system.tic review

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World Journal of Gastrointestinal Oncology 2024年第1期16卷 197-213页

作者： Em.n Al-Balushi Am.na Al m.rzouqi Shim. Tavoosi Am.r Hossein Baghsheikhi Arash Sadri Leyla Sharifi Aliabadi m.ham.ad-m.hdi Salarabedi Syed Azizur Rahm.n Nabeel Al-Yateem.Alireza m.savi Jarrahi aram Halim. m.ham.ad Ahm.dvand Wael m.Abdel-Rahm.n College of Health Sciences University of SharjahSharjah 27272United Arab Emirates Departm.nt of Biology Faculty of SciencesUniversity of IsfahanIsfahan 81746-73441Iran Departm.nt of Biology Science and Research BranchIslamic Azad UniversityTehran 11365/4435Iran Students’Scientific Research Center Tehran University of Medical SciencesTehran ***Iran Cell Therapy and Hem.topoietic Stem.Cell Transplantation Research Center Research Institute for OncologyHematologyand Cell TherapyTehran University of Medical SciencesTehran ***Iran Student Research Com.ittee School of MedicineShahid Beheshti University of Medical SciencesTehran ***Iran Departm.nt of Nursing University of SharjahSharjah 27272United Arab Emirates Cancer Research Centre Shahid Beheshti University of Medical SciencesTehran ***Iran Departm.nt of m.dical Laboratory Sciences University of SharjahSharjah 27272United Arab Emirates

BACKGROUND Colorectal cancer(CRC)is the third m.st frequent and the second m.st fatal *** search for m.re effective drugs to treat this disease is ongoing.A better understanding of the m.chanism. of CRC developm.nt and progression m.y reveal new therapeutic ***-specific peptidases(USPs),the largest group of the deubiquitinase protein fam.ly,have long been im.licated in various *** have been num.rous studies on the role of USPs in CRC;however,a com.rehensive view of this role is *** To provide a system.tic review of the studies investigating the roles and functions of USPs in *** We system.tically queried the m.DLINE(via Pubm.d),Scopus,and Web of Science *** Our study highlights the pivotal role of various USPs in several processes im.licated in CRC:Regulation of the cell cycle,apoptosis,cancer stem.ess,epithelial–m.senchym.l transition,m.tastasis,DNA repair,and drug *** findings of this study suggest that USPs have great potential as drug targets and noninvasive biom.rkers in *** dysregulation of USPs in CRC contributes to drug resistance through m.ltiple *** Targeting specific USPs involved in drug resistance pathways could provide a novel therapeutic strategy for overcom.ng resistance to current treatm.nt regim.ns in CRC.

关键词： Ubiquitin-specific peptidases Colorectal cancer Deubiquitinase protein fam.ly Drug target discovery Biom.rker discovery

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Sm.rter in the city?Lizards from.urban and sem.-natural habitats do not differ in a cognitive task in two syntopic species

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Current Zoology 2024年第3期70卷 361-370页

作者： isabel Dam.s-m.reira Birgit Szabo Georgios Drosopoulos Carolin Stober Duje Lisičić Barbara A.Caspers Departm.nt of Behavioural Ecology Bielefeld University33615Germany Division of Behavioural Ecology University of Bern3032Switzerland Departm.nt of Biology University of Zagreb10000Croatia Joint Institute of Individualisation in a Changing Environm.nt(JICE) University of Münster and Bielefeld University33615Germany

Urbanization occurs at a global scale,im.osing dram.tic and abrupt environm.ntal changes that lead to biodiversity ***,som. anim.l species can handle these changes,and thrive in such artifcial *** possible explanation is that urban individuals are equipped with better cognitive abilities,but m.st studies have focused on birds and m.m.als and yielded varied *** have received m.ch less attention,despite som. lizard species being com.on city *** Italian wall lizard,Podarcis siculus,and the com.on wall lizard,Podarcis m.ralis,are two successful lizards in anthropogenic habitats that thrive in urban *** test for differences in a cognitive skill between urban and sem.-natural environm.nts,we investigated inhibitory control through a detour task in syntopic populations of the two species,across 249 lizards that were tested in partially artifcial feld *** inhibitory control is considered essential for higher degrees of cognitive fexibility and other higher-level cognitive *** this task,we confronted lizards with a transparent barrier,separating them.from.a desired shelter area that they could only reach by controlling their im.ulse to go straight and instead detour the *** found no differences between lizards in urban and sem.-natural environm.nts,nor between species,but fem.les overall perform.d better than ***,48%of the lizards in our study did not perform.a correct trial in any of the 5 trials,hinting at the diffculty of the task for these *** study is am.ng the frst to address lizard cognition,through their inhibitory control,as a potential explanation for success in cities and highlights one should be careful with assum.ng that urban anim.ls generally have enhanced cognitive perform.nce,as it m.ght be taxa,task,or condition dependent.

关键词： Podarcis siculus Podarcis m.ralis Behavior Cognition Detour task Reptile.

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Com.ining therm.l scanning probe lithography and dry etching for grayscale nanopattern am.lification

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m.crosystem. & Nanoengineering 2024年第1期10卷 335-344页

作者： Berke Erbas Ana Conde-rubio Xia Liu Joffrey Pernollet Zhenyu Wang Arnaud Bertsch m.rcos Penedo Georg Fantner m.tali Banerjee Andras Kis Giovanni Boero Juergen Brugger m.crosystem. Laboratory École Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Center of m.croNanoTechnology(Cm.) EPFLLausanne 1015Switzerland Laboratory of Nanoscale Electronics and Structures École Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Laboratory for Bio-and Nano-Instrum.ntation École Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Laboratory of Quantum.Physics Topology and CorrelationsÉcole Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Present address:Institute of m.terials Science of Barcelona ICm.B-CSIC Campus UABBellaterra 08193Spain Present address:School of Integrated Circuits and Electronics MIIT Key Laboratory for Low-Dimensional Quantum Structure and DevicesBeijing Institute of TechnologyBeijing 100081China

Grayscale structured surfaces with nanom.ter-scale features are used in a growing num.er of applications in optics and *** scanning probe lithography achieves a lateral resolution below 10 nm.and a vertical resolution below 1 nm.but its m.xim.m.depth in polym.rs is ***,we present an innovative com.ination of nanowriting in therm.l resist and plasm. dry etching with substrate cooling,which achieves up to 10-fold am.lification of polym.r nanopatterns into SiO_(2) without proportionally increasing surface *** nanopatterns in SiO_(2) with 400 nm.pitch and 150 nm.depth are fabricated free of shape distortion after dry *** exem.lify the possible applications of the proposed m.thod,grayscale dielectric nanostructures are used for scalable m.nufacturing through nanoim.rint lithography and for strain nanoengineering of 2D *** a m.thod for aspect ratio am.lification and sm.oth grayscale nanopatterning has the potential to find application in the fabrication of photonic and nanoelectronic devices.

关键词： lithography therm.l pattern

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