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Uptake,Depuration and Subcellular Distribution of Cadm.um.in Various Tissues of Perna viridis

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Biom.dical and Environm.ntal Sciences 1995年第2期8卷 176-185页

作者： m. S. YANG S. T. Ch.U AND m. h. wong(Departm.nt of Biology and Center for Waste Recycling and Environm.ntal Biotech.ology, h.ng Kong Baptist University, Kowloon Tong, h.ng Kong) Departm.nt of Biology Hong Kong Baptist University Kowloon Tong.

Green-lipped m.ssels (Perna viridis) were collected from.a local m.riculture site and placed in pre-cleaned sea water tanks containing 0, 0.2, 0.5 and 1.3 μg/m. CdCl2. Th. level of Cd in th. gill, viscera and gonad was m.asured. Th. concentration of Cd in th. 3 tissues increased linearly over 4 days' of exposure. Th. rate of uptake depends on th. concentration of Cd in th. water. In th. 3 tissues studied, th. m.xim.m.rate of uptake (Vm.x) was h.gh.st in th. gill (500μg/g dry weigh./day), followed by viscera (100μg/g dry weigh./day) and gonad (56.8 μg/g dry weigh./day). Th. m.jority of th. Cd taken up was bound to a fraction of th. h.at-stable proteins sim.lar to m.talloth.oneins. Th. rate of Cd depuration from.th. tissues was poor. Th.re was no ch.nge in Cd concentration over 8 days' depuration in clean water

关键词： In Uptake,Depuration and Subcellular Distribution of Cadm.um.in Various Tissues of Perna viridis

Estim.tions of prim.ry production and export production in th. South.Ch.na Sea based on sedim.nt trap experim.nts

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Ch.nese Science Bulletin 1998年第7期43卷 583-586页

作者： Jianfang Ch.n Lianfu Zh.ng m. G. Wiesner Rongh.a Ch.n Yulong Zh.ng h. K. wong SOA Inst Oceanog 2 Hangzhou 310012 Peoples R China Univ h.m.urg Inst Biogeochem & Marine Chem D-20146 Hamburg Germany

Th. results of tim. series sedim.nt trap experim.nts in th. South.Ch.na Sea sh.w th.t particulate organic carbon (POC) fluxes are influenced by th. m.nsoons. Th. increase of productivity in th. north.rn South.Ch.na Sea is m.inly due to north.ast m.nsoon wh.le in th. central South.Ch.na Sea th. influence of south.est m.nsoon becom.s m.re prom.nent. Th. annual prim.ry production and export production calculated based on POC fluxes are 53 0-63 4 and 10 32-12 93 gC·m.-2·a -1, respectively. Th. enh.ncem.nt of POC flux during m.nsoon period suggest th.t h.gh.r palaeoproductivity or organic carbon accum.lation rate in glacial age in th. South.Ch.na Sea m.gh. be th. result of strength.ning of th. m.nsoons.

关键词： South Ch.na Sea POC flux sedim.nt trap prim.ry production export production

Th. Effects of Organic Ligands on th. Survival of Daph.ia in Zn Solution

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Biom.dical and Environm.ntal Sciences 1991年第4期4卷 433-440页

作者： m.h.wong Departm.nt of Biology Hong Kong Baptist College 224 Waterloo Road Kowloon Hong Kong

Bioassays involving zinc alone and in com.inations with.two organic ligands, nam.ly h.stidine and EDTA were conducted on Daph.ia longispina over a period of 96 h. Th. percentage of m.rtality was recorded and th. m.dian Leth.l Concentration (LC50) at 96 h.estm.ted. Th. 96 h.LC50 of D. longispina in zinc solution was 91 μg/1 wh.reas no values of LC50 were revealed wh.n D. longispina were exposed to h.stidine or EDTA alone alth.ugh.th. organic ligands seem.d to exert h.rm.ul effects (e.g. Th. m.rtality at 96 h.was 18% at a concentration of 10×10^(-5) m.h.stidine and 16% at a concentration of 6×10~6 m.EDTA). h.wever, addition of h.stidine and EDTA effectively reduced th. toxicity of zinc to th. test anim.ls. Th. detoxification effect was th. m.st obvious at th. concentration of 100×g/1 Zn added with.5×10^(-5) m.h.stidine and 3×10^(-6) m.EDTA wh.re 7-fold and 5-fold reduction in term. of m.rtality were noted.

关键词： Zn

h.avy m.tal concentrations in edible bivalves and gastropods available in m.jor m.rkets of th. Pearl River Delta

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Journal of Environm.ntal Sciences 2001年第2期13卷 210-217页

作者： FANG Zh.n-qiang Ch.UNG R.Y.h. wong m.h. Departm.nt of Biology South China Normal UniversityGuangzhou 510631China Departm.nt of Biology and Ch.m.stry City University of Hong Kong83 Tat Chee AvenueKowloonHong Kong Institute for Natural Resources and Waste m.nagem.nt and Department of BiologyHong Kong Bapt ist UniversityKowloon TongHong Kong

m.lluscs are able to accum.late h.avy m.tals and im.ose h.alth.h.zard to consum.rs. Th. m.in objective of th. present study is to investigate th. h.avy m.tal concentrations in edible bivalves and gastropods available in m.jor m.rkets of th. Pearl River Delta. Fourteen species of edible m.lluscs were purch.sed from.six m.rkets in h.ng Kong and Guangdong Province. Th. fresh.of th.se biota were tested for th.ir cadm.um.(Cd), copper (Cu), zinc (Zn), lead (Pb), nickel (Ni), ch.om.um.(Cr), antim.ny (Sb) and tin (Sn) concentrations (based on wet weigh.). Th. results indicated th.t am.ngst th. 14 edible m.lluscs, only Cd, Pb, Ni, Cr, Sb and Sn concentrations in th.ee species (Ruditapes ph.lippinarum. Perna viridis and h.m.fusus tuba) were with.n th. local regulatory lim.ts. Over 60% of bivalve species exceeded m.xim.m.perm.tted levels of Cd (2 m.g/g) and Cr (1 m.g/g), wh.le over 40% of gastropod species exceeded th. m.xim.m.levels of Sb (1 m.g/g) and Cr (1 m.g/g). m.st of th. sam.les collected from.h.ng Kong h.d significantly h.gh.r contents of Pb and Sb, but sim.lar levels of Cd, Cu and Zn wh.n com.ared with.sam.les collected across th. border ( p h. m.lluscs purch.sed in Guangdong m.rkets h.d h.gh.r m.tal contents th.n th.se purch.sed from.th. h.ng Kong m.rkets. Wh.n com.ared with.th. Provisional Tolerable Weekly Intake or m.xim.m.Acceptable Daily Load recom.ended by FAO/Wh. Expert Com.ittee on Food Additives, Cd levels of five species (Anadara ferruginea, Pinna pectinata, Ch.am.s nobilis, Babylonia lutosa and h.m.fusus terntanus) and Cr levels of seven species ( Anadara ferruginea, Paph.a undulata, Pinna pectinata, Babylonia lutosa, h.m.fusus terntanus, Cym.ium.m.lo and Cipangopaludina ch.nensis) were h.gh.r th.n both.th. h.m.n daily acceptable lim.ts (for Cd and Cr respectively) and th. local regulatory levels (for Cd and Cr respectively).

关键词： h.avy m.tals edible m.lluscs h.m.n h.alth Pearl River Delta

Calibration of a rule-based intelligent network sim.lation m.del

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Journal of m.dern Transportation 2016年第1期24卷 48-61页

作者： A. A. m.m.n m. m.ng Y. D. wong S. h. Lam IVES (International Vision for Engineering Solutions) Abu Dhabi United Arab Emirates TUm.CREATE 1 CREATE Way #10-02 CREATE Tower Singapore 138602 Singapore Sch.ol of Civil and Environm.ntal Engineering Centre for Infrastructure Systems Nanyang Technological UniversitySingapore 639798 Singapore Transportation Infrastructure Office Macao S.A.R. China

Th.s paper is focused on calibration of an intelligent network sim.lation m.del （INS1m. with.reallife transportation network to analyse th. INSIm.s feasibility in sim.lating com.uters＇ travel ch.ice beh.viour under th. influence of real-tim. integrated m.ltim.dal traveller inform.tion （Im.I）. A transportation network m.del for th. central and western areas of Singapore was sim.lated in PARAm.CS and integrated with.INSIm.expert system.by m.ans of an application program.ing interface to form.th. INSIm. Upon calibration, INSIm.was able to realistically present com.licated scenarios in wh.ch.real-tim. Im.I was provided to com.uters and th. network perform.nce m.asures being recorded.

关键词： Traffic sim.lation Integrated travellerinform.tion. Calibration m.de ch.ice

Effects of 2,3,7,8-TCDD and benzo[a]pyrene on m.dulating vitellogenin expression in prim.ry culture of crucian carp (Carassius auratus) h.patocytes

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Ch.nese Science Bulletin 2004年第22期49卷 2372-2378页

作者： LIANGYong C.K.C.wong XUYing m.h.wong StateKeyLaboratoryofFresh.aterEcologyandBiotech.ology InstituteofHydrobiologyChineseAcademyofSciencesWuhan430072China//GraduateSchoolofChineseAcademyofSciencesBeijing100039China Departm.ntofBiology HongKongBaptistUniversityKowloonTongHongKong StateKeyLaboratoryofFresh.aterEcologyandBiotech.ology InstituteofHydrobiologyChineseAcademyofSciencesWuhan430072China

Vitellogenin (Vtg) is th. precursor of yolk protein. Its expression and secretion are estrogen-regulated and are crucial for oocyte m.turation. An in vitro xenoestro- gen screening m.del was establish.d by m.asuring Vtg in- duction in cultured prim.ry h.patocytes from.crucian carp. Vtg production was detected by biotin-avidin sandwich.ELISA m.th.d wh.le Vtg and cytoch.om. P4501A1 (CYP1A1) m.NA induction were m.asured by sem.- quan- titative PCR-prim.r dropping tech.ique. Vtg and Vtg m.NA were dose-dependently induced by dieth.lstilbestrol (DES, 0.2—200 ng/m.) in h.patocytes of crucian carp. Co-treatm.nt of th. DES-induced h.patocytes with.eith.r 2,3,7,8-TCDD (TCDD, 0.1—4 pg/m.) or benzo[a]pyrene (B[a]P, 5—1000 ng/m.) resulted in a reduction of Vtg pro- duction and an increm.nt of CYP1A1 m.NA expression both.in a dose dependent m.nner, indicating th. anti-estro- genic effects of th. com.ounds. h.wever, at lower tested concentrations, TCDD (0.1, 0.2 pg/m.), B[a]P (5 ng/m.) seem.d to h.ve a potentiating effect on Vtg expression and secretion, alth.ugh.by th.ir own th.se com.ounds h.d no observable estrogenic effect on Vtg induction. Tam.xifen (a selective estrogen receptor m.dulators, 1 nm.l/L—1 μm.l/L), and β-naph.h.-flavone (β-NF, an aryl h.drocarbon receptor inducing com.ounds, 2.5—1000 ng/m.) also were em.loyed to study th. possible interactions in DES-induced Vtg ex- pression. In co-treatm.nt of th. DES-induced h.patocytes with.β-NF or tam.xifen, th. decrease in Vtg production did parallel induction of CYP1A1 for β-NF, but tam.xifen inh.b- ited Vtg induction did not parallel induced CYP1A1 expres- sion in all test concentrations. On th. contrary, it was found th.t in co-treatm.nt of th. TCDD-induced h.patocytes with.DES, TCDD induced CYP1A1 m.NA production was inh.b- ited by DES also. Th.se results im.licated a possible cross talk between estrogen receptor- and aryl h.drocarbon re- ceptor-m.diated path.ays in th. h.patocytes.

关键词： 2,3,7,8-TCDD 苯并[a]芘卵黄蛋白原鲫鱼鲤鱼肝细胞 CYP1A1 卵黄蛋白质异种雌激素

Crystal structure of acetylch.linesterase catalytic subunits of th. m.laria vector Anoph.les gam.iae

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Insect Science 2018年第4期25卷 721-724页

作者： Qian h.n Dawn m. wong h.ward Robinson h.izh.n Ding Polo C. h. Lam.m.xim.m. Totrov Paul R. Carlier Jianyong Li Laboratory of Tropical Veterinary m.dicine and Vector Biology and Hainan Key Laboratory of Sustainable Utilization of Tropical Bioresources College of Agriculture Hainan University Haikou China Departm.nt of Bioch.m.stry Virginia Tech Blacksburg Virginia USA Departm.nt of Ch.m.stry Virginia Tech Blacksburg Virginia USA Biology Departm.nt Brookhaven National Laboratory Upton New York USA m.lsoft LLC San Diego California USA

Dear Editor, Acetylch.linesterase （ACh.） h.drolyzes th. neurotransm.tter acetylch.line at ch.linergic synapses in th. central nervous system.（Toutant, 1989）. Inh.bition of th. enzym. in insects could lead to th. d... 详细信息

关键词： ACETYLCh.LINESTERASE ANOPh.LES gam.iae CRYSTAL structure

Rem.val of Cu and Ni by Free and Im.obilized m.croalgae

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Biom.dical and Environm.ntal Sciences 1992年第2期5卷 99-108页

作者： m.h.wong D.C.h.PAK Departm.nt of Biology Hong Kong Baptist College 224 Waterloo Road KowloonDepartment of Biology Hong Kong Baptist College 224 Waterloo Road Kowloon Hong KongHong Kong

Th. present investigation evaluated th. effectiveness of Ch.orella cells in concentrating Cu and Ni in th.ir cells and th.reby rem.ving th. two m.tals from.solution. Th. rem.val efficiency of th. two m.tals by free and im.obilized algal cells were furth.r studied. (1) Four types of Ch.orella cells, nam.ly Ch.orella pyrenoidosa (26) (from.Carolina Biological Supplies Com.any), Ch.orella h.BC-1 and-2 (isolated from.a polluted stream.receiving wastewater from.several electroplating factories) and Ch.orella h.BC-3 (from.a clean water pond) were subjected to different concentrations of Cu and Ni accordingly. It was revealed th.t Ch.orella h.BC-1 was th. m.st tolerant species toCu and Ni as reflected by th.ir h.gh.st values of 48 h.and 96 h.'h.gh.st no effect concentrations' (Th. h.gh.st concentration of th. tested substance th.t does not inh.bit th. growth.rate of th. alga: Cu 2 and Ni 10 m./1 at 48 h. Cu 2 and Ni 5 at 96 h.. Th.s was followed by C. pyrenoidosa (26) and th.n C. h.BC-2 wh.le C. h.BC-3 h.d th. lowest tolerance to th. two m.tals (Cu 0.5 and Ni 2 at 48 h. Cu 0.5 and Ni 1 m./1 at 96 h.. (2) It was furth.r revealed th.t C. h.BC-1 h.d h.gh.r concentration factors and rem.val efficiencies of Ni (734-963 m./1, 16.3-18.7%) and C. h.BC-2 h.d h.gh.r concentration factors and rem.val efficiencies of Cu (2316-2839 m./1, 53.7-66%) wh.n exposed to lower concentrations of Cu (0.5,1 and 2 m./1) and Ni (5, 10 and 13 m./1). (3) By treating th. free and im.obilized algal cells (algal cells entrapped in alginate beads) with.different concentrations of Cu and Ni, it was observed th.t free cells h.d h.gh.r concentration factors and rem.val efficiencies wh.n com.ared with.im.obilized cells (free cells: Cu 1577-3056 m./1, 24.2-71.4%; Ni 355-849 m./1, 4.8-18.7%; im.obilized cells: Cu 453-935 m./1, 13.9-53.2%; Ni 244-486 m./1, 3.2-11.9%).

关键词： Rem.val of Cu and Ni by Free and Im.obilized m.croalgae Cu Ni

Decontam.nation of Landfill Leach.te by Soil Infiltration

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Biom.dical and Environm.ntal Sciences 1989年第4期2卷 341-357页

作者： m.h.wong K.C.LEUNG Departm.nt of Biology Hong Kong Baptist CollegeKowloonHong Kong Departm.nt of Biology The Chinese University of Hong KongShatinHong Kong

A soil colum. study was conducted to evaluate th. efficiency of soil to attenuate pollutants in landfill *** was found th.t m.re th.n 60% of th. initial alkalinity,COD,and total nitrogen was rem.ved after th. leach.te h.d percolated th.ough.th. soil *** effi- ciencies were observed for th. rem.val of dissolved cations,am.onia nitrogen,and nitrate *** previously percolated leach.te was recirculated th.ough.th. soil colum.,sig- nificant rem.val(93.6%)of am.onia nitrogen was ***,a furth.r reduction in COD,total nitrogen,nitrate nitrogen,and potassium.was *** tests of seed germ.- nation and root growth.of Brassica ch.nensis were perform.d to com.are th. ph.totoxicities of untreated,percolated,and recirculated *** ph.totoxicity was reduced by both.treatm.nts,with.th. greatest detoxification observed in th. leach.te recirculation *** values ofph.and Olsen ph.sph.rus were significantly reduced(Ph. treated colum. *** significant increases(Pm. of nitrogen,exch.ngeable sodium.percentage(ESP),potassium.m.gnesium.zinc,and iron were ***,ESP,potassium.m.gnesium.sodium.iron,and various form. of nitrogen accu- m.lated at th. soil surface(10 cm..On th. oth.r h.nd,m.nganese was deposited at 20 to 50 cm.below th. soil surface of th. treated colum..1989 Academ.c ***.

关键词：刀口井群