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Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIm

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World Journal of Gastroenterology 2008年第8期14卷 1231-1236页

作者： Sufi HZ rahman m Golam Azam m anisur rahman mS Arfin m mahbub Alam Tareq m Bhuiyan Nasim Ahmed motiur rahman Shamsun Nahar mS Hassan Department of Immunology Bangladesh Institute of Research and Rehabilitation in Diabetes Endocrine and Metabolic Disorders Dhaka Bangladesh Department of Gastrointestinal Hepatobiliary and Pancreatic Diseases Bangladesh Institute of Research and Rehabilitation in Diabetes Endocrine and Metabolic Disorders Dhaka Bangladesh Department of Pathology Ibrahim MedicalCollege Dhaka Bangladesh Laboratory Sciences Division International Centre for Diarrhoeal Diseases Research Bangladesh Dhaka Bangladesh

AIm:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIm and conventional ELISA for the diagnosis of H pylori infection in adult dyspeptic patients. mETHODS:Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA (AccuBindTm ELISA, monobind, USA), ICT (Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore), and immunoblot (Helico Blot 2.1, Genelabs Diagnostics, Singapore) assays. ICT and immunoblot kits cover CIm among other parameters and their performance with and without CIm was evaluated separately. RESULTS:Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIm were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIm were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively. CONCLUSION:Immunoblot with CIm had the best performance. ICT with CIm was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of H pylori infection.

关键词： H pylori ELISA Immunochromatographic test Immunoblot Current infection marker

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Financial management for custom hire service of tractor in Bangladesh

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International Journal of Agricultural and Biological Engineering 2013年第3期6卷 28-33页

作者： anisur rahman most.Latifunnahar md monjurul Alam Department of Farm Power and machinery Bangladesh Agricultural UniversityMymensingh-2202Bangladesh

The main purpose of the study was to determine the economic parameters and effective schedules for tractor *** data from various sources were used in this *** essential data were collected from primary sources through *** costs were calculated and project financial profitability was determined by four major factors on farm financial measurement techniques,namely,benefit-cost ratio(BCR),net present value(NPV),internal rate of return(IRR),and payback *** the economic use,land topography and cropping pattern was developed to effective scheduling for tractor *** operating cost of tractor with implement was found to be US$9.25 per *** replacement of the existing tractor on expiry of economic life,the entrepreneur has to save an amount of US$219 per year in a bank *** on the operating cost,annual savings for replacement and a profit margin for the entrepreneur,the rent-out charge of the tractor is estimated at US$11.58 per *** 10%interest rate,the NPV of the tractor at existing condition is US$*** NPV of tractor indicates that tractor entrepreneurship is considered financially sound and the project is financially viable,with an average IRR of 36.96%.This is because IRR of the tractor was higher than the bank interest rate and it is highly profitable from the viewpoint of individual *** Payback period of tractor with implement was determined as 2.03 *** minimum tenure for an economic use of a common tractor used in agriculture is about 6310 *** this critical use,the utilization of a tractor is economical for a tractor *** on the cropping pattern two major and one minor turn-around periods are available in between cropping seasons for tilling *** time available for tilling of land is estimated about 140 days at 12 hours a day in a *** strengthen the existing capacity of the tractor customer hire service entrepreneurs an

关键词： tractor operating cost economic parameter effective scheduling tractor entrepreneur Bangladesh

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Influence of magnetic Force on the Flow Stability Based on Energy Gradient Theory

Influence of Magnetic Force on the Flow Stability Based on E...

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作者： anisur rahman 浙江理工大学

学位级别：硕士

The stability of the flow under the magnetic force is a special problem in fluid *** investigation of flow of fluids in ducts,diffusers and also other parts of various machines in the existence of the magnetic field is important for many technological *** this thesis,the influence of magnetic force on the flow stability in rectangular duct,diffuser,and an airfoil has been studied and the energy gradient theory is used to provide the theoretical analysis of the stability of the magnetohydrodynamics(mHD)*** the first part of this thesis,the flow in a rectangular duct with different Hartmann(Ha)number is *** SImPLE algorithm and the finite volume method are used to solve a system of equations and then the energy gradient theory is used to study the(associated)stability of mHD *** to the energy gradient theory,in pressure driven flow,K represents the ratio of energy gradient in the transverse direction and the energy loss due to viscosity in streamline *** with large value of K will lose its stability earlier than that with small value of *** flow stability of mHD flow for different Hartmann(Ha)number,from Ha=1 to 40,at the fixed Reynolds number,Re=190 are *** simulation is validated firstly against the simulation in *** results show that,with the increasing Ha number,the centerline velocity of the rectangular duct with mHD flow decreases and the absolute value of the gradient of total mechanical energy along the streamwise direction *** maximum of K appears near the wall in both the coordinate axis of the *** the energy gradient theory,this position of the maximum of K would initiate flow instability(if any)more than the other *** higher the Hartmann number is,the smaller the K value becomes,which means that the fluid becomes more stable in the presence of higher magnetic *** the Hartmann number increases,the K value in the parallel layer decreases more s

关键词： Numerical simulation rectangular duct diffuser,airfoil,magnetic force energy gradient theory stability mHD

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Social meanings of Attempted Suicide of men in Bangladesh

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Fudan Journal of the Humanities and Social Sciences 2022年第4期15卷 553-571页

作者： anisur rahman Khan Sipho Dlamini Kopano Ratele Department of Sociology East West UniversityDhaka1212Bangladesh Department of Psychology University of JohannesburgJohannesburgSouth Africa Centre for Critical and Creative Thought Stellenbosch UniversityStellenboschSouth Africa

Émile Durkheim’s Le Suicide(1897/2005)paved the way for the development of the sociology of ***,Durkheim’s positivistic approach in studying suicide was trenchantly challenged by,amongst others,Jack Douglas,who,in Social meanings of Suicide(1968),advocated extricating the social meanings of suicide from the interpretive tradition of social *** on Douglas’approach to the sociology of suicide,this paper reports and reflects on part of a study conducted across several rural areas of Jhenaidah,an area in Bangladesh highly affected by suicide,with 17 males who have attempted *** responses of the participants were interpreted with a view to understanding the social meanings associated with their suicide *** there was a diversity of individual experiences among the participants with regard to suicide attempts,the paper synthesises these and reflects on three themes which most closely represent the commonalities of experience of the 17 men,namely:(i)inability to provide;(ii)intimate relationship stress;and(iii)loss of social *** conclusion,the paper contributes to understanding suicide attempts by men as social meaningfully events,delineating the roles and complexities associated with constructions of masculine identities.

关键词： Suicide attempt men masculinity Social meaning Sociology of suicide

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Psorinum 6× triggers apoptosis signals in human lung cancer cells

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Journal of Integrative medicine 2016年第2期14卷 143-153页

作者： Jesmin mondal Asmita Samadder anisur rahman K huda-Bukhsh Cytogenetics and molecular Biology Laboratory Department of Zoology University of Kalyani Department of Zoology Dum Dum Motijheel College

OBJECTIVE： To provide in vitro evidence of Psorinum treatment against cancer cells in a controlled study. mETHODS： Effects of homeopathic Psorinum 6× on cell viability were initially determined in several cancer cell lines, including A549, Hep G2 and mCF-7, using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay, and an ethanol 6× control. The cell line that exhibited highest inhibition was selected and used in the following experiments. A range of Psorinum 6× doses was used to explore treatment effects on cell cycle arrest, cell death（apoptosis）, generation of reactive oxygen species（ROS） and change in mitochondrial membrane potential（mmP） using fl ow cytometry and fl uorescence microscopy, respectively. Expression of several signal proteins related to apoptosis and cell survival were quantified with Western blotting and confocal microscopy. Further, circular dichroism（CD） spectroscopy was used to determine possible drug-DNA interactions, as well as the induction of conformational changes. RESULTS： Treatment of cancer cell lines with Psorinum showed greater anticancer effects in A549 cells than in others. In A549 cells Psorinum treatment inhibited cell proliferation at 24 h after treatment, and arrested cell cycle at sub-G1 stage. It also induced ROS generation, mmP depolarization, morphological changes and DNA damage, as well as externalization of phosphatidyl serine. Further, increases in p53 expression, Bax expression, cytochrome c release, along with reduction of Bcl-2 level and caspase-3 activation were observed after Psorinum 6× treatment, which eventually drove A549 cells towards the mitochondria-mediated caspase-3-dependent pathway. CD spectroscopy revealed direct interaction of Psorinum with DNA, using calf thymus-DNA as ***： Psorinum 6× triggered apoptosis in A549 cells via both up- and down-regulations of relevant signal proteins, including p53, caspase-3, Bax and Bcl-2.

关键词： homeopathy Psorinum therapy lung neoplasms reactive oxygen species anticancer potential apoptosis drug-DNA interaction

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Ultra-highly diluted plant extracts of Hydrastis canadensis and marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro

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Journal of Integrative medicine 2015年第6期13卷 400-411页

作者： Santu Kumar Saha Sourav Roy anisur rahman Khud a-Bukhsh Cytogenetics and molecular Biology Laboratory Department of ZoologyUniversity of Kalyani Department of Entomology and Institute for Integrative Genome Biology University of California Riverside

OBJECTIVE： methylation-specific epigenetic process and gene expression profiles of HeLa cells treated with ultra-high dilutions （HDs） of two plant extracts, Hydrastis canadensis （HC-30） and marsdenia condurango （Condu-30）, diluted 106o times, were analyzed against placebo 30C （PI-30） for alterations in gene profiles linked to epigenetic modifications. mETHODS： Separate groups of cells were subjected to treatment of Condu-30, HC-30, and PI- 30 prepared by serial dilutions and succussions. Global microarray data recorded on Affymetrix platform, using 25-mer probes were provided by iLifeDiscoveries, India. Slides were scanned with 3000 7G microarray scanner and raw data sets were extracted from Cel （raw intensity） files. Analyses of global microarray data profile, differential gene expression, fold change and clusters were made using GeneSpring GX12.5 software and standard normalization procedure. Before microarray study, concentration of RNA （ng/IJL）, RIN value and rRNA ratio for all the samples were analysed by Agilant Bioanalyzer 2100. Reverse transcriptase polymerase chain reaction （RT-PCR） and quantitative RT- PCR were done for analyzing SmAD-4 expression. Fluorescence-activated cell sorting study was further made to elucidate fate of cells at divisional stages. methylation-specific restriction enzyme assay was conducted for ascertaining methylation status of DNA at specific sites. RESULTS： HDs of HC-30 and Condu-30 differentially altered methylation in specific regions of DNA and expression profiles of certain genes linked to carcinogenesis, as compared to PI-30. Two separate cut sites were found in genomic DNA of untreated and placebo-treated HeLa cells when digested with mcrBC, compared to a single cut observed in Condu-30-treated genomic DNA. SmAD-4 gene expression validated the expression pattern observed in microarray profile. methylation-specific restriction enzyme assay elucidated differential epigenetic modifications in drug-treated and control cells. CON

关键词： plant extracts homeopathy reactive oxygen species apoptosis gene expression epigenesis,genetic Smad4 protein

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Homeopathic Thuja 30C ameliorates benzo(a)pyreneinduced DNA damage,stress and viability of perfused lung cells of mice in vitro

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Journal of Integrative medicine 2013年第6期11卷 397-404页

作者： Avinaba mukherjee Naoual Boujedaini anisur rahman Khuda-Bukhsh Cytogenetics and molecular Biology Laboratory Department of ZoologyUniversity of Kalyani Boiron Laboratories

OBJECTIVE： To examine if the ultra-highly diluted homeopathic remedy Thuja 30C can ameliorate benzo（a）pyrene （BaP）-induced DNA damage, stress and viability of perfused lung cells of Swiss albino mice in vitro. mETHODS： Perfused normal lung cells from mice were cultured in 5% Roswell Park memorial Institute medium and exposed to BaP, a potent carcinogen, at the half maximal inhibitory concentration dose （2.2 μmol/L） for 24 h. Thereafter, the intoxicated cells were either treated with Thuja 30C （used against tumor or cancer） or its vehicle media, succussed alcohol 30C. Relevant parameters of study involving reactive oxygen species （ROS） accumulation, total glutathione （GSH） content, and generations of heat shock protein （hsp）-90 were measured; the cell viability and other test parameters were measured after treatment with either Thuja 30C or its vehicle media. Circular dichroism spectroscopy was performed to examine if Thuja 30C directly interacted with calf thymus DNA as target. For ascertaining if DNA damaged by BaP could be partially repaired and restituted by the remedy, 4＇,6-diamidino-2-phenylindole staining was performed. RESULTS： Thuja 30C increased cell viability of BaP-intoxicated cells significantly, as compared to drug-untreated or drug-vehicle control. A minimal dose of Thuja 30C significantly inhibited BaP-induced stress level, by down-regulating ROS and hsp-90, and increasing GSH content. Thuja 30C itself had no DNA-damaging effect, and no direct drug-DNA interaction. However, it showed quite striking ability to repair DNA damage caused by BaP. CONCLUSION： Thuja 30C ameliorates BaP-induced toxicity, stress and DNA damage in perfused lung cells of mice and it apparently has no effect on normal lung cells.

关键词： Thuja 30C benzo(a)pyrene reactive oxygen species oxidative stress HSP90heat-shock proteins in vitro

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modification of total and phosphorus mineralizing bacterial communities associated with Zea mays *** plant development and fertilization regimes

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Journal of Integrative Agriculture 2021年第11期20卷 3026-3038页

作者： XIN Yuan-yuan anisur rahman LI Hui-xiu XU Ting DING Guo-chun LI Ji College of Resources and Environmental Sciences China Agricultural UniversityBeijing 100193P.R.China Organic Recycling Institute(Suzhou) China Agricultural UniversitySuzhou 215128P.R.China

Harnessing the rhizospheric microbiome,including phosphorus mineralizing bacteria(PmB),is a promising technique for maintaining sustainability and productivity in intensive agricultural ***,it is unclear as to which beneficial taxonomic group populations in the rhizosphere are potentially associated with the changes in soil microbiomes shifted by fertilization ***,we analyzed the diversity and community structure of total bacteria and PmB in the rhizosphere of maize(Zea mays L.)grown in soils under 25 years of four fertilization regimes(compost,biocompost,chemical,or nonfertilized)via selective culture and Illumina sequencing of the 16S rRNA *** development explained more variations(29 and 13%,respectively)in the composition of total bacteria and PmB in the rhizosphere of maize than the different fertilization *** those genera enriched in the rhizosphere of maize,the relative abundances of Oceanobacillus,Bacillus,Achromobacter,Ensifer,Paracoccus,Ramlibacter,and Luteimonas were positively correlated with those in the bulk *** relative abundance of Paracoccus was significantly higher in soils fertilized by compost or biocompost than the other *** results were also observed for PmB affiliated with Ensifer,Bacillus,and *** plant development was the major factor in shaping the rhizospheric microbiome of maize,fertilization regimes might have modified beneficial rhizospheric microbial taxa such as Bacillus and Ensifer.

关键词： organic fertilization bacterial diversity phosphorus mineralizing bacteria(PmB) Zea mays L. rhizosphere

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Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: drug-DNA interaction and signaling cascade

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Journal of Integrative medicine 2014年第2期12卷 102-114页

作者： Samrat Ghosh Kausik Bishayee anisur rahman Khuda-Bukhsh Cytogenetics and molecular Biology Laboratory Department of ZoologyUniversity of Kalyani

OBJECTIVE： Oleanolic acid （OA） has been reported to have anticancer effects, but the extent of its cytotoxicity, its ability to interact with nuclear DNA, its action against skin melanoma, as well as the molecular mechanism of its action against cell proliferation and in support of cell death are still unexplored. This led us to examine the efficacy of OA, a bioactive compound isolated from Phytolacca decandra, on these issues in the present investigation. mETHODS： Studies related to analyses of cell viability, drug-DNA interaction, cell proliferation, cell cycle and epidermal growth factor receptor （EGFR） activity were performed. To investigate whether cells undergo apoptosis, studies like fluorescence microscopy, poly （ADP-ribose） polymerase （PARP） degradation, annexin V-fluorescein isothiocyanate/propidium iodide assay, alteration in mitochondrial membrane potential and activity of some relevant signaling proteins were performed. RESULTS： OA displayed a minimal and negligible cytotoxic effect on normal HaCaT cells （skin keratinocytes） and peripheral blood mononuclear cells but by contrast it reduced A375 cell viability significantly. OA interacted with nuclear DNA quickly after exposure. It acted as an anti- proliferative agent. It suppressed EGFR activity. OA administration led the cells to mitochondria- dependent caspase 3-mediated apoptosis. CONCLUSION： OA interacts with cellular DNA, inhibits proliferation possibly through modulating EGFR activity and induces mitochondria-dependent caspase 3-mediated apoptosis in A375 cells which would qualify it as a potent anticancer agent.

关键词： oleanolic acid skin melanoma drug-DNA interaction proliferation epidermal growth factor receptor apoptosis

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Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo

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Journal of Integrative medicine 2015年第1期13卷 34-44页

作者： Samrat Ghosh Sourav Sikdar Avinaba mukherjee anisur rahman Khuda-Bukhsh Cytogenetics and molecular Biology Laboratory Department of Zoology University of Kalyani

OBJECTIVE： Chemopreventive approach with natural products, particularly plants and plant-derived ones, is receiving increasing attention for their effective role against cancer without any palpable side effects. In this study, efficacy of ethanolic extract of Ruta graveolens （RG） on skin melanoma cells （A375） in vitro and on 7,12-dimethylbenz（a）anthracene （DmBA）-induced skin cancer in vivo has been tested in Swiss albino mice. mETHODS： Studies on cell viability, apoptosis and autophagy induction were conducted in vitro. To check apoptosis, assays like alteration in mitochondrial membrane potential, annexin V-fluorescein isothiocyanate/propidium iodide assay and immunoblot were performed. Fluorescence microscopic and immunoblot assays were performed to confirm autophagy induction. The effects of RG were determined by evaluating body weight, tumor incidence, tumor volume and tumor burden in mice. Enzymatic and non-enzymatic antioxidant status was assessed. The role of some relevant signaling proteins was also analyzed. RESULTS： RG caused death ofA375 cells through induction of caspase 3-mediated apoptosis and Beclin-l-associated autophagy. moreover, RG administration （75 mg/kg body weight） which showed no acute or chronic toxicity, showed significant reduction in the skin tumor burden of DmBA-painted mice. RG also demonstrated potent anti-lipid peroxidative and antioxidant functions during the course of skin cancer induction by DmBA. CONCLUSION： Chemopreventive potential of RG was demonstrated from overall results of this study indicating its possible use in therapeutic formulation of an effective drug to treat skin cancer.

关键词： Ruta graveolens plant extracts chemoprevention skin neoplasms apoptosis autophagy

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