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Effects of maternal methyl donor intake during pregnancy on ileum methylation and function in an intrauterine growth restriction pig model

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1173-1189页

作者： Yan Lin Jiangnan Wu Yong Zhuo Bin Feng Zhengfeng Fang Shengyu Xu Jian Li hua Zhao De Wu lun hua Lianqiang Che Key Laboratory of Animal Disease‑Resistance Nutrition Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina

Background Intrauterine growth retardation(IUGR)affects intestinal growth,morphology,and function,which leads to poor growth performance and high *** present study explored whether maternal dietary methyl donor(MET)supplementation alleviates IUGR and enhances offspring’s growth performance by improving intestinal growth,function,and DNA methylation of the ileum in a porcine IUGR *** Forty multiparous sows were allocated to the control or MET diet groups from mating until *** farrowing,8 pairs of IUGR and normal birth weight piglets from 8 litters were selected for sampling before suckling *** The results showed that maternal MET supplementation tended to decrease the IUGR incidence and increased the average weaning weight of ***,maternal MET supplementation significantly reduced the plasma concentrations of isoleucine,cysteine,urea,and total amino acids in sows and newborn *** also increased lactase and sucrase activity in the jejunum of newborn *** addition resulted in lower ileal methionine synthase activity and increased betaine homocysteine S-methyltransferase activity in the ileum of newborn *** methylation analysis of the ileum showed that MET supplementation increased the methyla-tion level of DNA CpG sites in the ileum of newborn ***-regulated differentially methylated genes were enriched in folic acid binding,insulin receptor signaling pathway,and endothelial cell *** contrast,up-regulated methylated genes were enriched in growth hormone receptor signaling pathway and nitric oxide biosyn-thetic *** Maternal MET supplementation can reduce the incidence of IUGR and increase the weaning litter weight of piglets,which may be associated with better intestinal function and methylation status.

关键词： Ileum Intrauterine growth restriction Methyl donor Methylation Sows

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Standardized ileal digestibility of amino acids in soybean meal fed to non-pregnant and pregnant sows

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Journal of Animal Science and Biotechnology 2024年第1期15卷 362-373页

作者： Ke Wang Ya Wang Lei Guo Yong Zhuo lun hua Lianqiang Che Shengyu Xu Ruinan Zhang Jian Li Bin Feng Zhengfeng Fang Xuemei Jiang Yan Lin De Wu Institute of Animal Nutrition Key Laboratory for Animal Disease‑Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130SichuanChina

Background Two studies were designed to determine standard ileal crude protein(CP)and amino acid(AA)digestibility of soybean meal(SBM)from different origins fed to non-pregnant and pregnant *** solvent-extracted SBMs from soybeans produced in the USA,Brazil,and China were *** Exp.1,eight different diets were created:a nitrogen(N)-free diet and 7 experimental diets containing SBM from different origins as the only N *** non-pregnant,multiparous sows were arranged in an 8×8 Latin square design(8 periods and 8 diets).In Exp.2,the diet formula was the same as in *** gestating sows(parity 3)were assigned to 4 different diets in a replicated 4×3 Youden square design(three periods and four diets)in mid-gestation and again in late-gestation *** When fed to non-pregnant and late-gestating sows,the standardized ileal digestibility(SID)of CP and most AAs from different SBM were not significantly different(P>0.05).When fed to mid-gestating sows,the SID values for Arg,His,Lys,Phe,Cys,Gly,Ser,and Tyr in SBM 1 were lower than in SBM 4 and 5(P<0.05),whereas SID for Leu from SBM 5 was higher than in SBM 1 and 4(P<0.05).SID values for Ile,Ala,and Asp from SBM 4 were lower than in SBM 1 and 5(P<0.05).Sows had significantly greater SID values for Lys,Ala,and Asp during mid-gestation when compared with late-gestation stages(P<0.05).Mid-gestating sows had greater SID value for Val and lower SID value for Tyr when compared with non-pregnant and late-gestating sows(P<0.01),whereas non-pregnant sows had significantly greater SID value for Met when compared with gestating sows(P<0.01).Conclusions When fed to mid-gestating sows,the SID values for most AAs varied among SBM *** SID values for Lys,Met,Val,Ala,Asp,and Tyr in SBM were affected by sow gestation *** findings provide a cornerstone for accurate SBM use in sow diets.

关键词： Amino acids Sows Soybean meal Standardized ileal digestibility

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煤基燃料燃烧颗粒物对颗粒捕集器沉积过程的影响

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汽车安全与节能学报 2024年第1期15卷 63-70页

作者：朱鑫昌刘帅王忠华伦帅石金江苏大学汽车与交通工程学院镇江212013 清华大学苏州汽车研究院吴江215200 清华大学智能绿色车辆与交通全国重点实验室(原汽车安全与节能国家重点实验室)北京100084

探讨了柴油机燃用代用燃料后,排气颗粒物结构特征的变化规律。依据柴油机台架试验,使用0%、5%、15%甲醇掺混比的F-T(Fischer–Tropsch)合成柴油,在标定工况下采集颗粒。用同步辐射小角散射分析方法测量颗粒物摩擦力、粒径等参数。基于... 详细信息

探讨了柴油机燃用代用燃料后,排气颗粒物结构特征的变化规律。依据柴油机台架试验,使用0%、5%、15%甲醇掺混比的F-T(Fischer–Tropsch)合成柴油,在标定工况下采集颗粒。用同步辐射小角散射分析方法测量颗粒物摩擦力、粒径等参数。基于实验数据,在EDEM软件中建立颗粒模型,模拟了颗粒碰撞沉积过程。结果表明:随甲醇掺混比的增加,甲醇、F-T柴油燃烧颗粒间摩擦力增加0.6 N,平均粒径增加2.44 nm。沉积过程中,颗粒捕集器(DPF)单元体非迎风面的沉积量急剧增加;颗粒沉积效率随沉积时间的增加而增加;随摩擦力增大、粒径增大,颗粒层厚度及颗粒链长度也随之增加。甲醇掺混比的改变使得颗粒整体向更多、更细的方向变化,燃料类型及掺混比的改变显著影响了颗粒在DPF载体上的沉积状态。

关键词：柴油机代用燃料甲醇、甲醇/F-T合成燃料颗粒物柴油机微粒捕集器(DPF) EDEM软件小角散射

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基于BP神经网络的固化红土抗压强度预测

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交通科学与工程 2024年第2期40卷 108-115页

作者：王硕唐正光华伦昆明理工大学建筑工程学院云南昆明650500

为分析不同掺量的偏高岭土与石灰共同掺入玄武岩残积红土中对土体的改良效果,本试验选取偏高岭土的掺量分别为0%、2%、4%、6%和8%,石灰的掺量分别为0%、2.5%、5.0%、7.5%和10.0%,同时掺入玄武岩残积红土中,制作25组不同固化红土,对其进... 详细信息

为分析不同掺量的偏高岭土与石灰共同掺入玄武岩残积红土中对土体的改良效果,本试验选取偏高岭土的掺量分别为0%、2%、4%、6%和8%,石灰的掺量分别为0%、2.5%、5.0%、7.5%和10.0%,同时掺入玄武岩残积红土中,制作25组不同固化红土,对其进行28 d无侧限抗压强度正交试验,并用MATLAB软件建立神经网络预测模型,预测固化红土养护28 d的抗压强度。研究结果表明:本模型预测误差最大为4.56%,拟合度为0.997,且本方法比常规回归分析法更简单、更准确,可预测不同固结材料和掺量的固化红土抗压强度,提高试验效率。

关键词：玄武岩残积红土 BP神经网络抗压强度强度预测模型预测误差

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柯乐猪METTL23基因多态性与繁殖性能的关联性分析

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农业生物技术学报 2024年第7期32卷 1577-1586页

作者：向进王春源罗华伦吴燕杨酸谭元成张依裕贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室/贵州大学香猪研究所黔西南州农产品质量安全检测中心

甲基转移酶23 (methyltransferase like 23, METTL23)对于猪(Sus scrofa)早期胚胎及组织器官发育过程具有重要促进作用，为了分析METTL23基因SNP位点的遗传效应对繁殖性状的影响，本研究以240头健康的经产柯乐猪母猪为研究对象，采用San... 详细信息

甲基转移酶23 (methyltransferase like 23, METTL23)对于猪(Sus scrofa)早期胚胎及组织器官发育过程具有重要促进作用，为了分析METTL23基因SNP位点的遗传效应对繁殖性状的影响，本研究以240头健康的经产柯乐猪母猪为研究对象，采用Sanger测序法对METTL23基因的SNP位点进行筛选鉴定，并利用SPSS 22.0软件分析SNP位点与柯乐猪的繁殖性状指标的关联性。结果表明，在柯乐猪METTL23基因共发现g.4804958G>T (第1内含子)、g.4805082C>T (第2外显子)和g.4806821A>G (第4外显子) 3个SNPs位点，均存在3种基因型，其中GT、CC和AA分别为对应突变位点的优势基因型，G、C和A分别为对应突变位点的优势等位基因，多态信息含量均表现为中度多态(0.25T位点对产活仔猪数、初生窝重和断奶仔猪数有显著影响(PG位点对初生窝重、初生平均重和断奶仔猪数有显著影响(PT和g.4806821A>G对柯乐猪繁殖性能有显著影响，双倍型H4H4 (GGCCGG)对繁殖性能有极显著影响，METTL23可以作为分子育种辅助标记。本研究为猪的遗传育种提供了理论参考。

关键词：甲基转移酶23(METTL23) 柯乐猪多态性关联性分析繁殖性能

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Absorption Spectrum of Neutral Krypton in the Near Infrared Region

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Chinese Journal of Chemical Physics 2019年第5期32卷 536-542页

作者： Ruo-yu Jiang Jia Ye lun-hua Deng Hai-ling Wang State Key Laboratory of Precision Spectroscopy East China Normal University Shanghai 200062 China

High-resolution absorption spectra of atomic krypton in the range of 11870-12700 cm-1 were recorded by employing concentration modulation absorption spectroscopy technique with a tunable single-mode cw Ti:Sapphire laser. The krypton atoms were excited to the absorbing energy states by discharge-burning in a mixture of helium and krypton. A total of 120 lines of neutral krypton were observed, among them 33 lines had already been classified in previous studies, 45 lines were newly classifi ed with the known energy levels, and 42 lines cannot be classified. These unclassi ed lines indicate that up to now unknown energy levels of Kr must exist. Further, an analysis of the unclassi ed lines to get possible new energy levels with a classi cation program is reported.

关键词： Neutral krypton Absorption spectrum Energy levels

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润滑油发动机试验中部件评分项目与方法研究

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润滑油 2024年第1期39卷 32-37,64页

作者：朱君君邢建强梁辰董红义俞毓先 Andrew Broff 华伦清华大学苏州汽车研究院(吴江) 江苏苏州215200 中国石化石油化工科学研究院有限公司北京100083 Intertek Automotive Research Texas 77056

用于润滑油评价的发动机试验结束后,拆解发动机并对核心部件进行评分的方法,已在润滑油性能评价中得到广泛应用。由于部件评分具有主观性,故需要通过标准方法规范部件评价项目与评分计算方法,实现主观评分的客观化,从而保证部分评分的... 详细信息

用于润滑油评价的发动机试验结束后,拆解发动机并对核心部件进行评分的方法,已在润滑油性能评价中得到广泛应用。由于部件评分具有主观性,故需要通过标准方法规范部件评价项目与评分计算方法,实现主观评分的客观化,从而保证部分评分的一致性。通过部件评分结合客观的部件测量以及油样理化测试分析,可以更全面的反映发动机状态及润滑油性能水平。文章回顾了国内外润滑油发动机试验中部件评分方法、分类和项目,重点介绍了活塞沉积物评分和油泥评分,分析了汽油机油和柴油机油产品规格中的部件评分考核项目的质量指标,为我国自主润滑油标准体系的发动机台架测试提供参考。

关键词：润滑油发动机试验评分沉积物油泥

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Microbial and metabolomic mechanisms mediating the effects of dietary inulin and cellulose supplementation on porcine oocyte and uterine development

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Journal of Animal Science and Biotechnology 2022年第3期13卷 907-922页

作者： Zhaoyue Men Meng Cao Yuechan Gong lun hua Ruihao Zhang Xin Zhu Lianchao Tang Xuemei Jiang Shengyu Xu Jian Li Lianqiang Che Yan Lin Bin Feng Zhengfeng Fang De Wu Yong Zhuo Animal Nutrition Institute Sichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130People’s Republic of China College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130People’s Republic of China

Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive *** objective of this study was to investigate the effects of DF intake level on oocyte maturation and uterine development,to determine the optimal DF intake for gilts,and gain microbial and metabolomic insight into the underlying mechanisms ***:Seventy-six Landrace×Yorkshire(LY)crossbred replacement gilts of similar age(92.6±0.6 d;mean±standard deviation[SD])and body weight(BW,33.8±3.9 kg;mean±SD)were randomly allocated to 4 dietary treatment groups(n=19);a basal diet without extra DF intake(DF 1.0),and 3 dietary groups ingesting an extra50%(DF 1.5),75%(DF 1.75),and 100%(DF 2.0)dietary fiber mixture consisting of inulin and cellulose(1:4).Oocyte maturation and uterine development were assessed on 19 d of the 2nd oestrous *** diversity of faecal samples was analysed by high-throughput pyrosequencing(16S r RNA)and blood samples were subjected to untargeted ***:The rates of oocytes showing first polar bodies after in vitro maturation for 44 h and uterine development increased linearly with increasing DF intake;DF 1.75 gilts had a 19.8%faster oocyte maturation rate and a 48.9 cm longer uterus than DF 1.0 gilts(P0.1%)increased linearly or quadratically with increasing DF *** metabolic analysis revealed significant changes in serum metabolites that were closely associated with microbiota,including serotonin,a gut-derived signal that stimulates oocyte ***:The findings provide evidence of the benefits of increased DF intake by supplementing inulin and cellulose on oocyte maturation and uterine development in gilts,and new microbial and metabolomic i

关键词： Dietary fiber Gilts Metabolomics Microbiota Oocyte maturation

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The “Bi-drifting” Subpulses of PSR J0815+0939 Observed with the Fivehundred-meter Aperture Spherical Radio Telescope

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Research in Astronomy and Astrophysics 2022年第2期22卷 195-203页

作者： lun-hua Shang Jun-Tao Bai Shi-Jun Dang Qi-Jun Zhi School of Physics and Electronic Science Guizhou Normal UniversityGuiyang 550001China School of Electronic and Optical Engineering Nanjing University of Science and TechnologyNanjing 210094China Guizhou Provincial Key Laboratory of Radio Astronomy and Data Processing Guizhou Normal UniversityGuiyang 550001China

We report the "Bi-drifting" subpulses observed in PSR J0815+0939 using the Five-hundred-meter Aperture Spherical radio Telescope(FAST). The observation at band from 1050 to 1450 MHz is evenly divided into two bands, i.e., the bands at center frequencies of 1150 and 1350 MHz. The mean pulse profiles and the "Bi-drifting"subpulses at these two bands are investigated. It is found that the pulse profiles at these two frequencies show four emission components, and the peak separations between four emission components decrease with the increase of frequency. In addition, the ratio of peak intensity of each component to the intensity of component Ⅳ at 1150 MHz is larger than that at 1350 MHz. We carry out an analysis of the longitude-resolved fluctuation spectrum and twodimensional fluctuation spectrum for each emission component, and find that the P3 of components Ⅰ,Ⅱ and Ⅲ is about 10.56, 10.57 and 10.59 s at 1150 and 1350 MHz. However, the reliable measurements of P3 of component IV and P2 for these four components were not obtained due to the low signal-to-noise ratio of observation *** pulse energy distributions at frequencies 1150 and 1350 MHz are presented, and it is found that no nulling phenomenon has been found in this pulsar. With our observation from the FAST, the "Bi-drifting" subpulse phenomenon of PSR J0815+0939 is expanded from 400 to 1350 MHz, which is helpful for the relevant researchers to test and constrain the pulsar emission model, especially the model of "Bi-drifting" subpulse.

关键词： (stars:)pulsars:general (stars:)pulsars:individual(PSR J0815+0939) radiation mechanisms:nonthermal

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Rotational Analysis of A2Πu-X2Πg System of 16O2+ Cation

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Chinese Journal of Chemical Physics 2014年第2期27卷 13-19页

作者： Jing Zhang Yuan-yue Zhu lun-hua Deng Yang-qin Chen East China Normal University and Department of Physics State Key Laboratory of Precision Spectroscopy

The Doppler-limited absorption spectrum of16O2+cation was observed in the region of11385-12100 cm-1by optical heterodyne velocity modulation absorption spectroscopy(OH-VMS). The transitions were assigned to the(2, 19),(3, 20), and(5, 21) bands in the second negative system(A2Πu-X2Πg). All the available lines measured using OH-VMS were global fitted in a nonlinear least-squares fitting procedure, and precise molecular constants(Bv, Av, Dv, pv, qv, γv) were obtained for the involved levels.

关键词： Oxygen molecule cation Second negative system Rotational resolution

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