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Neurophenotyping of Zebrafish larvae that Are Preconditioned with Anesthetic Agent

Journal of Behavioral and Brain Science 2016年第2期6卷 99-106页

作者： Julie l. Mustard Matthew A. Strope Christopher S. Havey lucy S. Wang Norbert W. Seidler Division of Basic Sciences Department of Biochemistry Kansas City University Kansas City MO USA Department of Anesthesiology University of Missouri/Saint Luke’s Hospital Kansas City MO USA

Anesthetics evoke a stress-response, upregulating heat shock genes. This neuroprotective response to proteotoxic stress represents preconditioning, a process by which neuronal tissue, previously exposed to anesthetics, is protected against future insult. It presumes a sub-lethal injury, affecting protein unfolding. Our hypothesis is: preconditioning evokes molecular events that result in downstream changes that offer a selective advantage in terms of neuronal function. We focused on the neurobehavioral aspects which we neurophenotyped. larval zebrafish were exposed to trifluoroethanol (TFE), an anesthetic mimetic, and tested for both individual and group behavioral markers of neuronal function. In bright/dark tests, we observed that TFE-exposed larvae spent more time in the dark area (typically an adult-like response) than control larvae. The response of TFE larvae to noise startle was directly opposite to that of controls. TFE larvae swam towards the source of the startle (into the bright zone), whereas control larvae swam away from the source of the startle (into the dark), typical of fear-response. The larvae also exhibited several differences in social behaviors, including synchronized schooling and shoaling behaviors. The TFE-group showed a greater number of synchronized events versus controls. The TFE-group also exhibited more shoaling events compared with controls. While the long-term effects have yet to be determined, these results shed light on the mechanism of anesthetic preconditioning. These complex zebrafish behaviors normally develop with age and therefore represent, in the TFE-exposed group, a pattern of accelerated maturation of neuronal function, which is the neurophenotype attributed to preconditioning.

关键词： Zebrafish Anesthetics Preconditioning Neurophenotype Trifluoroethanol

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刚地弓形虫表面抗原1、2 B细胞表位基因的融合表达和鉴定

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中国寄生虫学与寄生虫病杂志 2017年第6期35卷 575-579页

作者：王钊哲许瑞洪炀林矫矫陆珂李浩陈兆国石耀军吴思敏江嘉欣李嘉静朱传刚中国农业科学院上海兽医研究所农业部寄生虫学重点开放实验室国家防治动物血吸虫病专业实验室上海200241 江苏省动物重要疫病与人兽共患病防控协同创新中心扬州225009

目的构建刚地弓形虫(以下简称弓形虫)表面抗原(SAG)1、2 B细胞表位基因片段的原核表达系统,并对其免疫反应性进行分析。方法根据GenBank中已公布的编码弓形虫表面抗原的SAG基因序列,分析SAG1和SAG2基因的B细胞表位,设计并合成弓形虫表... 详细信息

目的构建刚地弓形虫(以下简称弓形虫)表面抗原(SAG)1、2 B细胞表位基因片段的原核表达系统,并对其免疫反应性进行分析。方法根据GenBank中已公布的编码弓形虫表面抗原的SAG基因序列,分析SAG1和SAG2基因的B细胞表位,设计并合成弓形虫表面抗原SAG1和SAG2蛋白的B细胞表位基因,将目的基因与p ET-28a(+)表达质粒连接,并转化至大肠埃希菌(Escherichia coli)Bl21(DE3)感受态细胞中,挑取完整单个菌落于含卡那霉素的lB液体培养基中,菌液进行PCR扩增、双酶切和测序鉴定;菌液振荡培养至吸光度(A450值)为0.6~0.8时,加入终浓度为1 mmol/l的异丙基-β-D-硫代半乳糖苷诱导表达,得到带有His标签的融合蛋白,进行十二烷基硫酸钠-聚丙烯酰胺凝胶(SDS-PAGE))电泳,对蛋白的可溶性进行分析。使用His亲和层析柱对表达的重组蛋白进行纯化,将带有His标签的纯化重组蛋白进行蛋白质印迹(Western blotting)分析和ElISA分析免疫反应性。结果构建的p ET28a(+)-SAG重组质粒,菌液经PCR、双酶切鉴定结果显示,在909 bp处出现特异性目的条带,与预期相符,经测序表明重组表达质粒构建成功。SDS-PAGE鉴定结果显示,重组蛋白rSAG相对分子质量(Mr)约50 000,为可溶性表达,与预期结果相符。Western blotting分析结果显示,重组蛋白可以与弓形虫感染小鼠阳性血清1∶100发生特异性结合。ElISA检测结果显示,重组蛋白稀释度为1∶1,阳性血清稀释度为1∶50时,阳性反应最明显,A450值为1.037 9。不同稀释倍数的重组蛋白r SAG与不同浓度的小鼠弓形虫阳性血清均可发生反应,并能很好的区分阴性和阳性血清。结论构建了弓形虫表面抗原SAG1和SAG2蛋白的B细胞表位基因的原核表达系统,重组蛋白rSAG为可溶性表达,并具有较好的免疫反应性。

关键词：刚地弓形虫抗原表位重组序列免疫反应性

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美国临床肿瘤学会IV期非小细胞肺癌化疗的临床实践指南更新

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中国肺癌杂志 2010年第3期13卷 171-189页

作者： Christopher G. AZZOlI Sherman Baker JR Sarah TEMIN William PAO Timothy AlIFF Julie BRAHMER David H. JOHNSON Janessa l. lASKIN Gregory MASTERS Daniel MIlTON luke NORDQUIST David G. PFISTER Steven PIANTADOSI Joan H. SCHIllER Reily SMITH Thomas J. SMITH John R. STRAWN David TRENT Giuseppe GIACCONE 丁燕(翻译) 南娟(翻译) 刘谦(翻译) 周清华(校对) 陈军(校对) From the Memorial Sloan-Kettering Cancer Center New York NY Virginia Commonwealth University Massey Cancer Center Richmond American Society of Clinical Oncology Alexandria Virginia Cancer Center Richmond VA Vanderbilt-Ingram Cancer Center Nashville TN Northwest Oncology & Hematology Associates Coral Springs FL Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University Baltimore National Cancer Institute Bethesda MD Helen F. Graham Cancer Center Newark DE Hematology/Oncology of Indiana PC Indianapolis IN Nebraska Cancer Specialists PC Omaha NE Samuel Oschin Comprehensive Cancer Institute Los Angeles CA University of Texas Southwestern Medical Center Dallas TX British Columbia Cancer Agency Vancouver British Columbia Canada 天津医科大学总医院天津市肺癌研究所天津市肺癌转移与肿瘤微环境重点实验室

本文旨在为IV期非小细胞肺癌患者的治疗提供更新版推荐。本文资料检索源自2002年以来公布的相关随机试验文献。此指南范围限于化疗与生物治疗。更新委员会对这些文献进行了总结并提供了推荐更新。162篇文献符合标准被纳入参考。本推荐... 详细信息

本文旨在为IV期非小细胞肺癌患者的治疗提供更新版推荐。本文资料检索源自2002年以来公布的相关随机试验文献。此指南范围限于化疗与生物治疗。更新委员会对这些文献进行了总结并提供了推荐更新。162篇文献符合标准被纳入参考。本推荐基于可改善总生存期的治疗方法。仅改善无进展生存期的治疗方法推动了对毒性及生存质量的监测。对于体力状态评分为0分或1分患者的一线治疗,可推荐以铂类为基础的细胞毒性药物的两药联用。对铂类治疗有禁忌的患者,可采用非铂类细胞毒性两药联合。对于体力状态评分为2分的患者,单一细胞毒性药物即可。对于疾病进展或经过4个周期的治疗仍对治疗无反应的患者,应停止一线细胞毒性化疗。即使在6个周期后患者对治疗仍有反应,亦应停止两药细胞毒性化疗。对于伴有明确的表皮生长因子受体(epidermal growth factor receptor,EGFR)突变的患者,可推荐一线采用吉非替尼治疗;对于EGFR突变为阴性或不明确的患者,细胞毒性化疗更佳。除具有特定临床特征的患者外,可推荐贝伐单抗与卡铂-紫杉醇联用。对于通过免疫组化证实EGFR阳性的肿瘤患者,可推荐西妥昔单抗与顺铂-长春瑞滨联用。多西紫杉醇、厄洛替尼、吉非替尼或培美曲塞被推荐作为二线治疗。对于未曾接受过厄洛替尼或吉非替尼治疗的患者,可推荐厄洛替尼作为三线治疗。现有数据不足以推荐常规三线采用细胞毒性药物。已有的证据也不足以推荐常规应用分子标记物选择化疗。

关键词：临床实践指南非小细胞肺癌选择化疗美国临床肿瘤学会细胞毒性药物表皮生长因子受体 receptor 肺癌患者

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Culture, Detection and Identification of Dengue Virus in C6/36 Aedes albopictus, llC-MK2, MFl and Vero Cell lines

Culture, Detection and Identification of Dengue Virus in C6/...

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Third Congress of the Asian-Pacific Organization for Cell Biology

作者： Filipinas F. Nalividad Giselle V. Espiritu James l. Dungca Ramil R.Noche Corazon C.Buerano Ronald R,Matias Institute of Biology College of ScienceUniversity of the PhilippinesDilimanQuezon City 1101PhilippinesResearch and Biotechnology DivisionSt. Luke’s Medical Center279 E. Rodriguez Sr. Blvd.Quezon CityPhilippines

Four cell lines C6/36 Aedes albopictus, llC-MK2, MFl and Vero, were used for isolation and culture of dengue virus from serum of patients diagnosed with dengue fever in the Philippines. Fifty microliter (50 μl) of se...

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洛杉矶社区相关性耐甲氧西林金黄色葡萄球菌感染所致的坏死性筋膜炎

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世界核心医学期刊文摘（皮肤病学分册） 2005年第8期1卷 2-3页

作者： Miller l.G. Perdreau- Remington F. Rieg G. 惠海英 Dr.Division of Infectious Diseases Harbor- UCLA Medical Center L. A. Biomed. Inst. At Harbor- UCLA Torrance CA United States

BACKGROUND: Necrotizing fasciitis is a life- threatening infection requiring urgent surgical and medical therapy. Staphylococcus aureus has been a very uncommon cause of necrotizing fasciitis, but we have recently noted an alarming number of these infections caused by community- associated methicillin- resistant S. aureus (MRSA). METHODS: We reviewed the records of 843 patients whose wound cultures grew MRSA at our center from January 15,2003, to April 15,2004. Among this cohort, 14 were identified as patients presenting from the community with clinical and intraoperative findings of necrotizing fasciitis, necrotizing myositis, or both. RESUlTS: The median age of the patients was 46 years (range, 28 to 68), and 71 percent were men. Coexisting conditions or risk factors included current or past injection- drug use (43 percent); previous MRSA infection, diabetes, and chronic hepatitis C (21 percent each); and cancer and human immunodeficiency virus infection or the acquired immunodeficiency syndrome (7 percent each). Four patients (29 percent) had no serious coexisting conditions or risk factors. All patients received combined medical and surgical therapy, and none died, but they had serious complications, including the need for reconstructive surgery and prolonged stay in the intensive care unit. Wound cultures were monomicrobial for MRSA in 86 percent, and 40 percent of patients (4 of 10) for whom blood cultures were obtained had positive results. All MRSA isolates were susceptible in vitro to clindamycin, trimethoprim- sulfamethoxazole, and rifampin. All recovered isolates belonged to the same genotype (multilocus sequence type ST8, pulsed- field type USA300, and staphylococcal cassette chromosome mec type IV [SCCmecIV]) and carried the Panton- Valentine leukocidin (pvl), lukD, and luke genes, but no other toxin genes were detected. CONClUSIONS: Necrotizing fasciitis caused by community- associated MRSA is an emerging clinical entity. In areas in which community- a

关键词：坏死性筋膜炎金黄色葡萄球菌血培养阳性体外药敏试验慢性丙型肝炎整形外科手术吸毒史肌炎经验性治疗药物治疗

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糖尿病孕妇超重和肥胖对妊娠结局的影响

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世界核心医学期刊文摘（妇产科学分册） 2005年第10期1卷 3-4页

作者： langer O Yogev Y Xenakis E.M.J Brustman l 朱国栋 Department of Obstetrics and Gynecology St. Luke's-Roosevelt Hospital Center 1000 Tenth Avenue New York NY 10019 United States.Dr

Objective: We sought to investigate the relationship between prepregnancy weight, treatment modality (diet or insulin), level of glycemic control, and pregnancy outcome. Study design: We recruited women with gestational diabetes (GDM) from inner city prenatal clinics. All women were instructed in the use of an intensified management protocol using memory reflectance meters. Outcomes were analyzed according to maternal prepregnancy body mass index (BMI, kg/m 2) categories: normal weight (BMI 18.5- 24.9), overweight (BMI 25- 29.9), and obese (BMI ≥ 30), and by diet or insulin therapy and glycemic control (mean blood glucose l = good control). Pregnancy outcome variables included a composite outcome (at least 1 of the following: neonatal metabolic complications, large- for- gestational age or macrosomic infants, NICU admission for >24 hours, and the need for respiratory support) (not including oxygen therapy). In addition to composite outcome, a bivariate analysis was performed for each single variable, including preeclampsia and cesarean section delivery. Results: Four thousand and one women were enrolled. Obese women who achieved targeted levels of glycemic control had comparable pregnancy outcomes to normal weight and overweight women only when they were treated with insulin. Normal weight women treated with diet therapy who achieved targeted levels of glycemic control had good outcomes, but obese women treated with diet therapy who achieved targeted levels of glycemic control, nevertheless, had a 2- to 3- fold higher risk for adverse pregnancy outcome when compared with overweight and normal weight patients with well- controlled GDM. Women with GDM who failed to achieve established levels of glycemic control had significantly higher adverse pregnancy outcomes in all 3 maternal weight groups. Conclusion: In obese women with BMI ≥ 30 with GDM, achievement of targeted levels of glycemic control was associated with enhanced outcome only in women treated with

关键词：妊娠结局糖尿病孕妇血糖控制水平孕前体重妊娠期糖尿病妊娠不良结局大于胎龄儿平均血糖目标水平正常体重

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关于大麻素-1受体阻断剂利莫那班对超重或肥胖患者体重和心脏代谢危险因素影响的随机对照试验:RIO-北美研究

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世界核心医学期刊文摘（心脏病学分册） 2006年第6期2卷 6-7页

作者： Pi-Sunyer F.X. Aronne l.J. Heshmati H.M. 任付先 Obesity Research Center St. Luke's- Roosevelt Hospital WH1020 1111 Amsterdam Ave New York NY 10025 United States Dr.

Context: Rimonabant, a selective cannabinoid- 1 receptor blocker, may reduce body weight and improve cardiometabolic risk factors in patients who are overweight or obese. Objective: To compare the efficacy and safety of rimonabant with placebo each in conjunction with diet and exercise for sustained changes in weight and cardiometabolic risk factors over 2 years. Design, Setting, and Participants: Randomized, double- blind, placebo- controlled trial of 3045 obese(body mass index ≥ 30) or overweight(body mass index >27 and treated or untreated hypertension or dyslipidemia) adult patients at 64 US and 8 Canadian clinical research centers from August 2001 to April 2004. Intervention: After a 4- week single- blind placebo plus diet(600 kcal/d deficit) run- in period, patients were randomized to receive placebo, 5 mg/d of rimonabant, or 20 mg/d of rimonabant for 1 year. Rimonabant- treated patients were rerandomized to receive placebo or continued to receive the same rimonabant dose while the placebo group continued to receive placebo during year 2. Main Outcome Measures: Body weight change over year 1 and prevention of weight regain during year 2. Additional efficacy measures included changes in waist circumference, plasma lipid levels, and other cardiometabolic risk factors. Results: At year 1, the completion rate was 309(51% ) patients in the placebo group, 620(51% ) patients in the 5 mg of rimonabant group, and 673(55% ) patients in the 20 mg of rimonabant group. Compared with the placebo group, the 20 mg of rimonabant group produced greater mean(SEM) reductions in weight(- 6.3 [0.2] kg vs - 1.6[0.2] kg; Plevel of triglycerides(percentage change,- 5.3[1.2] vs 7.9 [2.0]; Plevel of high- density lipoprotein cholesterol(percentage change, 12.6 [0.5] vs 5.4 [0.7]; Placebo group during y

关键词：随机对照试验肥胖患者受体阻断剂危险因素心脏代谢大麻素体重超重北美限制饮食

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沃尔巴克氏菌(Wolbachia)结合放射线的应用现场清除白纹伊蚊种群

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中华实验和临床病毒学杂志 2019年第5期33卷 494-494页

作者： Xiaoying Zheng Dongjing Zhang Yongjun li Cui Yang Yu Wu Xiao liang Yongkang liang Xiaoling Pan linchao Hu Qiang Sun Xiaohua Wang Yingyang Wei Jian Zhu Wei Qian Ziqiang Yan Andrew GParker jeremie r.l.Gilles Kostas Bourtzis Jeremy Bouyer Moxun tang Bo Zheng Jianshe Yu Julian liu Jiajia Zhuang Zhigang Hu Meichun Zhang Juntao Gong Xiaoyue Hong Zhoubing Zhang lifeng lin Qiyong liu Zhiyong Hu Zhongdao Wu luke Anthony Baton Ary AHoffmann Zhiyong Xi 毛伟(编译) 曾琴(编译) 潘晓玲(编译) 梁国栋(审校) 不详湖南师范大学医学院湖南长沙410013

《自然》杂志近期发表论文介绍中国、美国、国际原子能机构和澳大利亚科学家通过合作于2014—2017年在中国广东省广州市南沙区和番禺区2个江中沙洲岛屿,开展清除白纹伊蚊即登革病毒主要传播蚊虫的现场研究。该研究结合昆虫绝育技术(ster... 详细信息

《自然》杂志近期发表论文介绍中国、美国、国际原子能机构和澳大利亚科学家通过合作于2014—2017年在中国广东省广州市南沙区和番禺区2个江中沙洲岛屿,开展清除白纹伊蚊即登革病毒主要传播蚊虫的现场研究。该研究结合昆虫绝育技术(sterile insect technique,SIT),通过释放携带沃尔巴克氏菌(Wolbachia)的白纹伊蚊雄蚊,而达到靶向消灭现场野生白纹伊蚊的目的。研究结果显示,此项技术可年平均消灭现场白纹伊蚊83%~94%雌性成蚊和94%蚊卵,在部分区域实现了野生白纹伊蚊种群的灭绝。

关键词：白纹伊蚊国际原子能机构登革病毒放射线沃尔巴克应用现场 Wolbachia

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红曲对中度血脂升高美国人调节血脂作用的临床研究

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上海预防医学 2013年第9期25卷 501-506页

作者：陆继红 Kermit Bonovich Harry Colfer Michael Davidson Carlos A.Dujovne David l.Fried Mitchell Greenspan Ronald P.Karlsberg Stephen P.King Craig F.laForce Marc litt J.Robert McGhee 曾润海朱佳石谈宁芝华茂北京临床药理研究中心北京100088 Healthy Options Burns Clinic Rush-Presbyterian-St. luke's Medical Center Kansas University Medical Center and Kansas Foundation for Clinical Pharmacology Omega Medical Research lifemark Medical Center Cardiovascular Research Institute of So. CA Future Scripts North Carolina Clinical Research Jacksonville Heart Center Mediquest Research Group Nu Skin Center for Anti-aging Research 香港理工大学

[目的]评价在饮食控制的情况下红曲（RYRCholestin）对美国高血脂人群的降脂作用和安全性。[方法]开放标记，自身对照，多中心临床试验。187名美国高血脂人群入选（血清低密度脂蛋白胆固醇3．50—4．92mmol／l，总胆固醇5．18—7．25mmo... 详细信息

[目的]评价在饮食控制的情况下红曲（RYRCholestin）对美国高血脂人群的降脂作用和安全性。[方法]开放标记，自身对照，多中心临床试验。187名美国高血脂人群入选（血清低密度脂蛋白胆固醇3．50—4．92mmol／l，总胆固醇5．18—7．25mmol／l；男116人，女71人），162入完成试验。在整个试验中受试者遵照美国国家胆固醇教育计划的饮食要求。试验的前4周仅控制饮食，第5周至第12周连续服用8周RYRCholestin（每日2．4g）。[结果]经前4周饮食控制，血清脂质各指标无显著变化。口服RYRCholestin8周后，血清总胆固醇、低密度脂蛋白胆固醇和三酰甘油分别降低16．6％，24．O％和25．2％，高密度脂蛋白胆固醇升高14．3％（P〈0．001）。显效率为79．0％，有效率为97．5％。停服14d（2周）后，血脂各指标回到试验前水平。试验中共发现可能与服用产品相关的、轻度的29项不良反应。[结论]RYRCholestin的耐受性良好，它可降低血清总胆固醇、低密度脂蛋白胆固醇和三酰甘油，升高血清高密度脂蛋白胆固醇。停服2用后受试者的血脂各项指标均恢复到试验前水平。

关键词：红曲高脂血症低密度脂蛋白胆固醇三酰甘油高密度脂蛋白胆固醇

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联用tPA、肝素及手术再通治疗肝移植术后下腔静脉栓塞

引用

世界核心医学期刊文摘（胃肠病学分册） 2005年第9期1卷 51-51页

作者： Mindikoglu A.l. Miller J.S. Borge M.A. Van Thiel D.H. 陈琳 St. luke's Medical Center 2801 W. Kinnickinnic River Pkwy. Milwaukee WI 53215 United States

Complete inferior vena cava (IVC) thrombosis can be a lethal complication in a liver transplant recipient. The case of a 52-year-old liver transplant recipient, who developed complete IVC as well as left iliofemoral thrombosis, is reported. After treatment with combined tissue plasminogen activator (tPA)-and heparin, the IVC was successfully recanalized with sharp dissection, balloon dilatation, and stent placement.

关键词：肝移植 tPA 下腔静脉髂静脉支架植入术

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