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Horticulture Research 2015年第1期2卷 310-316页

作者： Lindsay E Wyatt Susan R Strickler lukas A Mueller Michael Mazourek Department of Plant Breeding and Genetics Cornell UniversityIthacaNYUSA Boyce Thompson Institute for Plant Research Tower RoadIthacaNYUSA

Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary *** is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown *** provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally *** with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon *** candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait *** transcriptome represents a major advance in *** genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed *** studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource.

关键词： squash breeding cultivar

Structural Aspects of Multistep Phosphorelay- Mediated Signaling in Plants

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Molecular Plant 2016年第1期9卷 71-85页

作者： Blanka Pekarova Agnieszka Szmitkowska Radka Dopitova Oksana Degtjarik lukas Zidek Jan Hejatko Faculty of Science Institute of Chemistry and Biochemistry University of South Bohemia Branisovska 1760 370 05 Ceske Budejovice Czech Republic Faculty of Science Institute of Chemistry and Biochemistry University of South Bohemia 370 05 Ceske Budejovice Czech Republic

The multistep phosphorelay （MSP） is a central signaling pathway in plants integrating a wide spectrum of hormonal and environmental inputs and controlling numerous developmental adaptations. For the thor- ough comprehension of the molecular mechanisms underlying the MSP-mediated signal recognition and transduction, the detailed structural characterization of individual members of the pathway is critical. In this review we describe and discuss the recently known crystal and nuclear magnetic resonance structures of proteins acting in MSP signaling in higher plants, focusing particularly on cytokinin and ethylene signaling in Arabidopsis thaliana. We discuss the range of functional aspects of available structural infor- mation including determination of ligand specificity, activation of the receptor via its autophosphorylaUon, and downstream signal transduction through the phosphorelay. We compare the plant structures with their bacterial counterparts and show that although the overall similarity is high, the differences in structural de- tails are frequent and functionally important. Finally, we discuss emerging knowledge on molecular recog- nition mechanisms in the MSP, and mention the latest findings regarding structural determinants of signaling specificity in the Arabidopsis MSP that could serve as a general model of this pathway in all higher plants.

关键词： multistep phosphorelay structure histidine kinase phosphotransfer protein response regulator

Reduction of Transient Engine-Out NO_(x)-Emissions by Advanced Digital Combustion Rate Shaping

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Automotive Innovation 2020年第2期3卷 181-190页

作者： Daniel Neumann lukas Schäfers Paul Muthyala Jakob Andert Stefan Pischinger Institute for Combustion Engines RWTH Aachen UniversityAachenGermany FEV Consulting GmbH Neuenhofstraße 18152078 AachenGermany

Modern diesel passenger cars already fulfill high demands regarding the reduction in NOx emissions through complex exhaust aftertreatment *** the consideration of real driving emissions,the reduction in NOx emissions in high transient engine operation becomes even more *** from increasing the complexity of exhaust aftertreatment systems,internal engine measures play a major *** approach to reducing NOx emissions described in this paper uses the precise control of the *** this purpose,the method of digital combustion rate shaping control is applied,which allows the realization of a predefined combustion by automatically adapting the injection profile during *** this work,this controller is extended in order to control the predefined combustion trace based on target NOx ***,the working principal of the state-of-the-art digital combusting rate shaping controller is *** the next step,the design and strategy of the extended control approach are explained and ***,its potential to reduce engine-out NOx emissions during transient driving situations is evaluated based on simulations of the *** is shown that the control concept fulfills the requirements and is able to effectively reduce high NOx peaks during transient operation.

关键词： Diesel engine Emission control Combustion rate shaping Closed loop combustion control NO_(x)Control

Blockchain-enabled decentralized identity management:The case of self-sovereign identity in public transportation

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Blockchain(Research and Applications) 2021年第2期2卷 50-67页

作者： lukas Stockburger Georgios Kokosioulis Alivelu Mukkamala Raghava Rao Mukkamala Michel Avital Department of Digitalization Copenhagen Business SchoolHowitzvej 60FrederiksbergDK-2000Denmark Department of Technology Kristiania University CollegeKirkegata 24-260153 OsloNorway

Identity management has been ripe for disruption over the past few years due to recurring incidents of data breaches that have led to personal information leaks and identity *** rise of blockchain technology has paved the way for the development of self-sovereign identity(SSI)—a new class of user-controlled resilient identity management systems that are enabled by distributed ledger *** paper examines how SSI management can be used in a public transportation sector that spans different operators in multiple ***,the paper explores how a blockchain-based decentralized identity management system can draw on the SSI framework to provide high-level security and transparency for all involved parties in public transportation ***,building on analyses of the existing public transportation ticketing solutions,we elicited requirements of a comparable system based on the SSI ***,we developed a low-fidelity prototype to showcase how passengers can utilize standardized travel credentials that are valid across different transportation networks in *** proposed system eliminates the need for multiple travel cards(i.e.,one for each transportation provider)and empowers individuals to have better control over the use of their identities while they utilize interoperable ticketing systems across ***,building on the public transportation case,we offer a proof-of-concept that shows how individuals can better manage their identity credentials via the SSI framework.

关键词： Identity management Self-sovereign identity Blockchain Distributed ledger Intelligent transport systems Payment systems infrastructure

Clinical algorithms for the prevention of variceal bleeding and rebleeding in patients with liver cirrhosis

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World Journal of Hepatology 2021年第7期13卷 731-746页

作者： Nikolaus Pfisterer lukas W Unger Thomas Reiberger Medizinische Abteilung für Gastroenterologie und Hepatologie Klinik Landstraße/Krankenanstalt RudolfstiftungVienna 1030Austria Vienna Hepatic Hemodynamic Lab Medical University of ViennaVienna 1090Austria Division of Visceral Surgery Department of General SurgeryMedical University of ViennaVienna 1090Austria Cambridge Institute of Therapeutic Immunology and Infectious Disease Jeffrey Cheah Biomedical CentreUniversity of CambridgeCambridge CB20AWUnited Kingdom Division of Gastroenterology and Hepatology Department of Medicine IIIMedical University of ViennaVienna 1090Austria Christian Doppler Laboratory for Portal Hypertension and Liver Fibrosis Medical University of ViennaVienna 1090Austria

Portal hypertension(PH),a common complication of liver cirrhosis,results in development of esophageal *** esophageal varices rupture,they cause significant upper gastrointestinal bleeding with mortality rates up to 20%despite state-of-the-art ***,prophylactic measures are of utmost importance to improve outcomes of patients with *** high-quality studies have demonstrated that non-selective beta blockers(NSBBs)or endoscopic band ligation(EBL)are effective for primary prophylaxis of variceal *** secondary prophylaxis,a combination of NSBB+EBL should be routinely *** esophageal varices develop and variceal bleeding occurs,standardized treatment algorithms should be followed to minimize bleeding-associated *** attention should be paid to avoidance of overtransfusion,early initiation of vasoconstrictive therapy,prophylactic antibiotics and early endoscopic ***-emptive transjugular intrahepatic portosystemic shunt should be used in all Child C10-C13 patients experiencing variceal bleeding,and potentially in Child B patients with active bleeding at *** use of carvedilol,safety of NSBBs in advanced cirrhosis(*** refractory ascites)and assessment of hepatic venous pressure gradient response to NSBB is *** the present review,we give an overview on the rationale behind the latest guidelines and summarize key papers that have led to significant advances in the field.

关键词： Portal hypertension Endoscopy Non-selective betablockers Transjugular intrahepatic portosystemic shunt

Optical leaky fin waveguide for long-range optical antennas on high-index contrast photonic circuit platforms

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Photonics Research 2023年第9期11卷 1570-1582页

作者： lukas VAN ISEGHEM WIM BOGAERTS Photonics Research Group Department of Information TechnologyGhent University-IMECGhentBelgium Center for Nano-and Biophotonics(NB Photonics) Ghent UniversityGhentBelgium

Long-distance light detection and ranging(LiDAR)applications require an aperture size in the order of 30 mm to project 200–300 *** generate such collimated Gaussian beams from the surface of a chip,this work presents a novel waveguide antenna concept,which we call an“optical leaky fin antenna,”consisting of a tapered waveguide with a narrow vertical“fin”on *** proposed structure(operating aroundλ=1.55μm)overcomes fundamental fabrication challenges encountered in weak apodized gratings,the conventional method to create an offchip wide Gaussian beam from a waveguide *** explore the design space of the antenna by scanning the relevant cross section parameters in a mode solver,and their sensitivity is *** also investigate the dispersion of the emission pattern and angle with the *** simulated design space is then used to construct and simulate an optical antenna to emit a collimated target intensity *** show inherent robustness to crucial design parameters and indicate good scalability of the *** and challenges to fabricate this device concept are also *** novel antenna concept illustrates the possibility to integrate long optical antennas required for long-range solid-state LiDAR systems on a high-index contrast platform with a scalable fabrication method.

关键词： waveguide antenna optical

Harnessing coherence of area decomposition and semantic shared spaces for task allocation in a robotic fleet

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Information Processing in Agriculture 2014年第1期1卷 23-33页

作者： Domagoj Drenjanac Slobodanka Dana Kathrin Tomic lukas Klausner Eva Kühn The Telecommunications Research Center Vienna(FTW) Donau-City-Strasse 11220 ViennaAustria Institute of Computer Languages Vienna University of TechnologyArgentinierstrasse 81040 ViennaAustria

Task allocation is a fundamental problem in multi-robot systems where heterogeneous robots cooperate to perform a complex mission.A general requirement in a task allocation algorithm is to find an optimal set of robots to execute a certain *** paper presents the work that harnesses an area decomposition algorithm,and a space-based middleware to facilitate task allocation process in unstructured and dynamic *** reduce spatial interference between robots,area decomposition algorithm divides a working area into cells which are then dynamically assigned to *** addition,coordination and collaboration among distributed robots are realized through a space-based *** this purpose,the space-based middleware is extended with a semantic model of robot capabilities to improve task selection in terms of flexibility,scalability,and reduced communication overhead during task *** this way a framework which exploits the synergy of area decomposition and semantically enriched space-based approach is *** conducted performance tests in a specific precision agriculture use case focusing on the utilization of a robotic fleet for weed control introduced in the European Project RHEA–Robot Fleets for Highly Effective Agriculture and Forestry Management.

关键词： Task allocation Area decomposition Space-based computing Semantics Robotic fleet

Pores for thought:generative adversarial networks for stochastic reconstruction of 3D multi-phase electrode microstructures with periodic boundaries

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npj Computational Materials 2020年第1期6卷 971-981页

作者： Andrea Gayon-Lombardo lukas Mosser Nigel P.Brandon Samuel J.Cooper Department of Earth Science and Engineering Imperial College LondonLondon SW72BPUK Earth Science Analytics ASA Professor Olav Hanssens vei 7AStavanger 4021Norge Dyson School of Design Engineering Imperial College LondonLondon SW72DBUK

The generation of multiphase porous electrode microstructures is a critical step in the optimisation of electrochemical energy storage *** work implements a deep convolutional generative adversarial network(DC-GAN)for generating realistic nphase microstructural *** same network architecture is successfully applied to two very different three-phase microstructures:A lithium-ion battery cathode and a solid oxide fuel cell anode.A comparison between the real and synthetic data is performed in terms of the morphological properties(volume fraction,specific surface area,triple-phase boundary)and transport properties(relative diffusivity),as well as the two-point correlation *** results show excellent agreement between datasets and they are also visually *** modifying the input to the generator,we show that it is possible to generate microstructure with periodic boundaries in all three *** has the potential to significantly reduce the simulated volume required to be considered“representative”and therefore massively reduce the computational cost of the electrochemical simulations necessary to predict the performance of a particular microstructure during optimisation.

关键词： microstructure electrode phase

Targeting new ways for large-scale,high-speed surface functionalization using direct laser interference patterning in a roll-to-roll process

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International Journal of Extreme Manufacturing 2023年第3期5卷 569-583页

作者： Christoph Zwahr Nicolas Serey lukas Nitschke Christian Bischoff Ulrich Radel Alexandra Meyer Penghui Zhu Wilhelm Pfleging Laser Precision Manufacturing Fraunhofer Institute for Material and Beam Technology(IWS)DresdenGermany Topag Lasertechnik GmbH DarmstadtGermany Institute of Manufacturing Science and Engineering Technical University DresdenDresdenGermany Institute for Applied Materials—Applied Materials Physics(IAM-AWP) Karlsruhe Institute of Technology(KIT)Eggenstein-LeopoldshafenGermany

Direct Laser Interference Patterning(DLIP)is used to texture current collector foils in a roll-to-roll process using a high-power picosecond pulsed laser system operating at either fundamental wavelength of 1064 nm or 2nd harmonic of 532 *** raw beam having a diameter of 3 mm@1/e^(2) is shaped into an elongated top-hat intensity profile using a diffractive so-called FBS■-L element and cylindrical *** shaped beam is split into its diffraction orders,where the two first orders are parallelized and guided into a galvanometer *** deflected beams inside the scan head are recombined with an F-theta objective on the working position generating the interference *** DLIP spot has a line-like interference pattern with about 15μm spatial *** fluences of up to 8 J cm^(-2) were achieved using a maximum pulse energy of 0.6 ***,an in-house built roll-to-roll machine was *** this setup,aluminum and copper foil of 20μm and 9μm thickness,respectively,could be *** to current collector structuring coating of composite electrode material took *** case of lithium nickel manganese cobalt oxide(NMC 622)cathode deposited onto textured aluminum current collector,an increased specific discharge capacity could be achieved at a C-rate of 1℃.For the silicon/graphite anode material deposited onto textured copper current collector,an improved rate capability at all C-rates between C/10 and 5℃ was *** rate capability was increased up to 100%compared to reference *** C-rates between C/2 and 2℃,the specific discharge capacity was increased to 200 mAh g^(-1),while the reference electrodes with untextured current collector foils provided a specific discharge capacity of 100 m Ah g^(-1),showing the potential of the DLIP technology for cost-effective production of battery cells with increased cycle lifetime.

关键词： DLIP lithium-ion battery surface texturing copper aluminum

Biohybrid Micro-and Nanorobots for Intelligent Drug Delivery

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Cyborg and Bionic Systems 2022年第1期2022卷 187-199页

作者： Jinhua Li lukas Dekanovsky Bahareh Khezri Bing Wu Huaijuan Zhou Zdenek Sofer School of Medical Technology Beijing Institute of TechnologyBeijing 100081China Department of Inorganic Chemistry University of Chemistry and Technology PragueTechnicka 516628 Prague 6Czech Republic

Biohybrid mico-and nanorobots are integrated tiny machines from biological components and artificial *** can poss ess the advantages of onboard actuation,sensing,control,and implementation of multiple medical tasks such as targeted drug delivery,single-cell manipulation,and cell *** review paper is to give an overview of biohybrid micro-and nanorobots for smart drug delivery ***,a wide range of biohybrid micro-and nanorobots comprising different biological components are reviewed in ***,the applications of biohybrid mico-and nanorobots for active drug delivery are introduced to demonstrate how such biohybrid micro-and nanorobots are being exploited in the field of medicine and ***,key challenges to be overcome are discussed to pave the way for the clinical translation and application of the biohybrid micro-and nanorobots.

关键词： overcome artificial delivery