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Leukocyte-technetium-99m uptake in Crohn's disease:Does it show subclinical disease?

World Journal of Gastroenterology 2010年第3期16卷 365-371页

作者： Luciene G Mota luiz gv coelho Carlos JR Simal Maria LA Ferrari Clodomiro Toledo Josep Martin-Comin Simone OF Diniz Valbert N Cardoso Nuclear Engineering Department Federal University of Minas Gerais Gastro-enterology ALFA Institute Clinical HospitalFederal University of Minas Gerais Nuclear Medicine Felício Rocho HospitalMedicine FacultyFederal University of Minas Gerais Medicine Nuclear Center Bellvitge Hospital Radioisotope Laboratory Pharmacy FacultyFederal University of Minas Gerais

AIM:To evaluate inflammatory activity in patients with Crohn's disease (CD) using technetium-99m-hexamethylpropyleneamine oxime (99mTc-HMPAO) granulocyte ***: Twenty patients (7 male and 13 female) with CD and five healthy volunteers were selected for 99mTc-HMPAO granulocyte scintigraphy. The Crohn's Disease Activity Index (CDAI), blood tests and C-reactive protein (CRP) of each patient were performed 7 d before the scintigraphic images. The leukocytes were labeled according to the International Society of Radiolabeled Blood Elements (ISORBE) consensus protocol and the scintigraphic images, including single photon emission computed tomography, were obtained 30 min and 2 h after injection of the radiolabeled ***:The labeling yield of the leukocytes with the lipophilic complex 99mTc-HMPAO was 55.0%±10%. Six of the 20 patients (30%) presented congruent results for the three parameters investigated (CDAI, Scintigraphic Index and CRP). On the other hand, 14 patients (70%) did not show congruent results. There was no significant correlation between the indices analyzed according to the Spearman test (P>0.05,n=20).CONCLUSION: The results suggest that 99mTc-HMPAO-labeled leukocyte scintigraphy could be important for determining inflammatory activity in CD even in the absence of clinical symptoms.

关键词： Technetium-99m Hexamethylpropyleneamine oxime Leukocytes Inflammatory bowel disease Scintigraphy

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Clinical/Behavioral Monitoring of Rodents and Rabbits Undergoing Scientific Experiments

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Open Journal of Veterinary Medicine 2024年第5期14卷 91-109页

作者： luiz Cesar Cavalcanti Pereira Jhônata Willy Rocha coelho Hyago da Silva Medeiros Elidio Rita de Cássia dos Passos Ferraz Da Silva João Gabriel Regis Sobral Bárbara Alves de Brito Soledade Leandro Thomaz Vilela Isabele Barbieri Dos Santos Institute of Science and Technology in Biomodels (ICTB) Oswaldo Cruz Foundation (FIOCRUZ) Rio de Janeiro Brazil Laboratory Animal Facility of the Carlos Chagas Pavilion of the Animal Experimentation Center (CEA) Oswaldo Cruz Institute (IOC) Oswaldo Cruz Foundation (FIOCRUZ) Rio de Janeiro Brazil

Background: We currently have international and national guidelines regarding the assessment and monitoring of clinical signs and humane endpoints in animals used in teaching and research, which make the performance of these activities mandatory for any experiment and professional working in this area. Assigning the severity of a research experiment is the result of an analysis of records of observations of the animal’s behavior, and clinical signs. The aim of this study was to describe the importance of carrying out a severity assessment associated with clinical and behavioral monitoring of rodents and rabbits during experimentation to maintain the welfare of these animals undergoing scientific research. Methods: The literature search was carried out using the following terms: “Monitoring”;“Humane endpoints”;“Animal welfare”, “Rodents”;“Rabbits”, and as connectors “and”;“or”, in the following databases: PubMed;LILACS/BIREME and SciELO. Results: A total of 987 articles were identified in the databases, and 20 of these studies were included in this review. Conclusions: Humane endpoint protocols and procedure severity tables are of the utmost importance, both from an ethical point and to refine the results of research conducted on laboratory animals. They should be drawn up jointly by the teams responsible for the project and the maintenance of the animals during the research period, and the data obtained should be published so that the scientific community can have access to it, helping to disseminate these practices, as well as helping to draw up new procedures. Monitoring and evaluating the welfare and clinical condition of animals undergoing scientific research procedures is the responsibility of the professors, researchers, veterinarians, and animal facility coordinators. The Ethics Committee on the Use of Animals must monitor all the activities conducted with the animals, by inspecting the experimental procedures and the physical environment of the laboratory an

关键词： Monitoring Humane Endpoints Animal Welfare Experimental Design Laboratory Animals’ Investigations

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Heme as an inducer of cerebral damage in hemorrhagic stroke:potential therapeutic implications

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Neural Regeneration Research 2022年第9期17卷 1961-1962页

作者： luiz Ricardo da Costa Vasconcellos Pedro Moreno Pimentel-coelho Cellular Signalling and Cytoskeletal Function Laboratory The Francis Crick InstituteLondonUK Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de JaneiroInstituto Nacional de Ciência e Tecnologia em Medicina RegenerativaRio de JaneiroRJBrazil

Intracerebral hemorrhage(ICH)consists of the rupture of a cerebral artery leading to bleeding into the surrounding *** event has a primary phase of brain injury consisting of mechanical tissue damage due to the mass effect,followed by a secondary phase of brain injury triggered by the presence of blood components released at the site of bleeding(Bulters et al.,2018).Despite the high rates of mortality and morbidity from ICH,no effective treatment is available so far.

关键词： cerebral damage rupture

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Marigold(Tagetes erecta): The Potential Value in the Phytoremediation of Chromium

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Pedosphere 2017年第3期27卷 559-568页

作者： Lívia C.coelho Ana Rosa R.BASTOS Paulo J.PINHO Guilherme A.SOUZA Janice G.CARVALHO Viviane A.T.coelho luiz Carlos A.OLIVEIRA Rimena R.DOMINGUES Valdemar FAQUIN Soil Science Department Federal University of Lavras Chemistry Department Federal University of Minas Gerais

Environmental pollution with chromium （Cr） is harmful to humans, animals and plants, while in plants it causes diminished growth, anatomical alterations and death. In the present study, the potential value of marigold （Tagetes erecta） in the phytoremediation of Cr has been investigated. The randomized experimental design involved the exposure of plants to nutrient solutions containing 0.00, 0.04, 0.08, 0.12, 0.16 or 0.24 mmol L-1 Cr（III）. Chromium toxicity was observed at Cr（III） concentrations ≥ 0.12 mmol L-1 as demonstrated by diminished growth of the aerial parts and reduced density of the root system. Increasing Cr（III） concentrations in the nutrient solution resulted in a higher bioaccumulation of total Cr in the tissues, although translocation from roots to aerial parts was not efficient （maximum value of 25% at 0.12 mmol L-1 Cr（III））. The Cr bioaccumulation was up to 11-fold greater in roots than in the aerial parts. Tagetes erecta exhibited leaf plasticity when exposed to Cr, indicating the existence of a tolerance mechanism to Cr in this species. Chromium caused a reduction in xilem vases, resulting in a plastic effect in T. erecta leaves that increased the metal tolerance in culture solution. Tagetes spp. are potential Cr hyperaccumulators; at Cr（III） concentrations up to 0.12 mmol L-1, the plants accumulated levels above that proposed for hyperaccumulators and still maintained a considerable growth and even flourished. However, this study was conducted in nutrient solution, and studies on species confirmation as Cr hyperaccumulator should be conducted in soils for further clarification.

关键词： anatomical alterations heavy metal hyperaccumulor leaf anatomy toxicity

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Pentoxifylline enhances the protective effects of hypertonic saline solution on liver ischemia reperfusion injury through inhibition of oxidative stress

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Hepatobiliary & Pancreatic Diseases International 2015年第2期14卷 194-200页

作者： Vinicius Rocha-Santos Estela RR Figueira Joel A Rocha-Filho Ana MM coelho Rafael Soraes Pinheiro Telesforo Bacchella Marcel CC Machado luiz AC D'Albuquerque Department of Gastroenterology Laboratory of Medical Investigations LIM37 Discipline of Liver and Gastrointestinal Transplantation Hospital das Clinicas University of Sao Paulo School of Medicine Discipline of Anesthesiology Hospital das Clinicas University of Sao Paulo School of Medicine Department of Gastroenterology Laboratory of Medical Investigations LIM37 Discipline of Liver and Gastrointestina Hospital das Clinicas University of Sao Paulo School of Medicinel Transplantation

BACKGROUND：Liver ischemia reperfusion（IR）injury triggers a systemic inflammatory response and is the main cause of organ dysfunction and adverse postoperative outcomes after liver ***（PTX）and hypertonic saline solution（HTS）have been identified to have beneficial effects against IR *** study aimed to investigate if the addition of PTX to HTS is superior to HTS alone for the prevention of liver IR ***： Male Wistar rats were allocated into three groups. Control rats underwent 60 minutes of partial liver ischemia, HTS rats were treated with 0.4 mL/kg of intravenous 7.5% NaCl 15 minutes before reperfusion, and HPTX group were treated with 7.5% NaC1 plus 25 mg/kg of PTX 15 minutes before reperfusion. Samples were collected after reperfusion for determination of ALT, AST, TNF-α, IL-6, IL-10, mitochondrial respiration, lipid peroxidation, pulmonary permeability and myeloperoxidase. RESULTS： HPTX significantly decreased TNF-α 30 minutes after reperfusion. HPTX and HTS significantly decreased ALT,AST, IL-6, mitochondrial dysfunction and pulmonary myelo- peroxidase 4 hours after reperfusion. Compared with HTS only, HPTX significantly decreased hepatic oxidative stress 4 hours after reperfusion and pulmonary permeability 4 and 12 hours after reperfusion. CONCLUSION： This study showed that PTX added the beneficial effects of HTS on liver IR injury through decreases of hepatic oxidative stress and pulmonary permeability.

关键词： pentoxifylline hypertonic saline solution hepatic oxidative stress ischemia reperfusion injury pulmonary permeability

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Beneficial effects of adenosine triphosphate-sensitive K^+ channel opener on liver ischemia/reperfusion injury

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World Journal of Gastroenterology 2014年第41期20卷 15319-15326页

作者： Mateus Antunes Nogueira Ana Maria Mendona coelho Sandra Nassa Sampietre Rosely Antunes Patzina Fabiano Pinheiro da Silva luiz Augusto Carneiro D'Albuquerque Marcel Cerqueira Cesar Machado Department of Gastroenterology (LIM/37) Medical SchoolUniversity of Sao Paulo Department of Clinical Emergency (LIM/51) Medical SchoolUniversity of Sao Paulo

AIM: To investigate the effect of diazoxide administration on liver ischemia/reperfusion injury.

关键词： Liver ischemia/reperfusion Diazoxide K+ channel opener Mitochondrial ATP-sensitive potassium channel Liver mitochondria

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决定巴西Espinhaco生物圈保护区森林-草地变化的环境因素

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Journal of Plant Ecology 2023年第5期16卷 13-25页

作者： Thais Ribeiro Costa Cristiane coelho de Moura Leovandes Soares da Silva Anne Priscila Dias Gonzaga AndréRodrigo Rech Evandro luiz Mendonga Machado Department of Forest Sciences Federal University of Vales do Jequitinhonha e Mucuri-Campus JKRodovia MGT 367Km 583n 5.000Alto da JacubaDiamantina/MGCEP39100-000Brazil

关于热带雨林(对火敏感)和开放地层(耐火)之间边界所涉及过程的认识对于保护生物多样性和生态系统服务至关重要,尤其是在气候变化和人为压力增加的情况下。本文调查了位于巴西东南部的Espinhaco生物圈保护区内的森林岛屿和湿润草原同时... 详细信息

关于热带雨林(对火敏感)和开放地层(耐火)之间边界所涉及过程的认识对于保护生物多样性和生态系统服务至关重要,尤其是在气候变化和人为压力增加的情况下。本文调查了位于巴西东南部的Espinhaco生物圈保护区内的森林岛屿和湿润草原同时出现的主要环境因素。我们使用Costa(2018)布置的永久样地来收集森林岛屿的土壤变量(水分和化学性质)。对于湿润草原的采样,我们从岛屿边缘沿不同方向设置了4条30m的线。随后,我们在每条线上划了3个点,每个点相距10m,每个区域共12个点。我们还调查了处理火烧前后的植被覆盖情况,并对环境变量进行均值检验和主成分分析。研究结果显示,森林岛屿土壤中钾、盐基总量、阳离子交换容量和有机质的值均高于湿润草地。因此,两种植被类型之间的边界定义似乎主要与土壤肥力和水分梯度有关。在对这些地区进行火烧处理后,在岛屿边缘附近没有发现树木个体的再生。因此,这些研究结果表明,由于明确的土壤气候条件,森林岛屿无法扩张。因此,这些环境应该成为设计公共保护政策的目标焦点,因为它们增加了山地岩石草原植被景观的复杂性。

关键词：湿地土壤气候梯度岛屿森林综合火灾管理有机土

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NT157 has antineoplastic effects and inhibits IRS1/2 and STAT3/5 in JAK2^(V617F)-positive myeloproliferative neoplasm cells

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Signal Transduction and Targeted Therapy 2020年第1期5卷 2440-2449页

作者： Bruna Alves Fenerich Jaqueline Cristina Fernandes Ana Paula Nunes Rodrigues Alves Juan luiz coelho-Silva Renata Scopim-Ribeiro Priscila Santos Scheucher Christopher A.Eide Cristina E.Tognon Brian J.Druker Eduardo Magalhães Rego João Agostinho Machado-Neto Fabiola Traina Department of Medical Images Hematologyand Clinical OncologyUniversity of Sao Paulo at Ribeirao Preto Medical SchoolRibeirao PretoSao PauloBrazil Center for Cell-Based Therapy Sao Paulo Research FoundationRibeirao PretoSao PauloBrazil Knight Cancer Institute Oregon Health&Science UniversityPortlandORUSA Howard Hughes Medical Institute PortlandORUSA Department of Internal Medicine University of Sao Paulo Medical SchoolSao PauloBrazil Department of Pharmacology Institute of Biomedical Sciences of the University of Sao PauloSao PauloBrazil

Recent data indicate that IGF1R/IRS signaling is a potential therapeutic target in BCR-ABL1-negative myeloproliferative neoplasms(MPN);in this pathway,IRS2 is involved in the malignant transformation induced by JAK2^(V617F),and upregulation of IGF1R signaling induces the MPN ***157,a synthetic compound designed as an IGF1R-IRS1/2 inhibitor,has been shown to induce antineoplastic effects in solid ***,we aimed to characterize the molecular and cellular effects of NT157 in JAK2^(V617F)positive MPN cell lines(HEL and SET2)and primary patient hematopoietic *** JAK2^(V617F)cell lines,NT157 decreased cell viability,clonogenicity,and cell proliferation,resulting in increases in apoptosis and cell cycle arrest in the G2/M phase(p<0.05).NT157 treatment inhibited IRS1/2,JAK2/STAT,and NFκB signaling,and it activated the AP-1 complex,downregulated four oncogenes(CCND1,MYB,WT1,and NFKB1),and upregulated three apoptotic-related genes(CDKN1A,FOS,and JUN)(p<0.05).NT157 induced genotoxic stress in a JAK2/STAT-independent ***157 inhibited erythropoietin-independent colony formation in cells from polycythemia vera patients(p<0.05).These findings further elucidate the mechanism of NT157 action in a MPN context and suggest that targeting IRS1/2 proteins may represent a promising therapeutic strategy for MPN.

关键词： JAK2 IGF1R neoplasms STAT3

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Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

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World Journal of Gastroenterology 2012年第31期18卷 4162-4168页

作者： Raimundo Fernandes de Araújo Júnior luiz Alberto Lira Soares Cínthia Raquel da Costa Porto Ranniere Gurgel Furtado de Aquino Hugo Gon alo Guedes Pedro Ros Petrovick Tatiane Pereira de Souza Aurigena Antunes de Araújo Gerlane coelho Bernardo Guerra Department of Morphology/Postgraduate Program in Morphological Sciences Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of PernambucoRecife 50670-901PernambucoBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of Rio Grande do NorteNatal 59012-570Rio Grande do NorteBrazil Department of Production and control of drugs Federal University of Rio Grande do SulPorto Alegre 90610-000Rio Grande do SulBrazil Department of Food and Drug Administration Federal University of AmazonasManaus 69077-000AmazonasBrazil Department of Biophysics and Pharmacology Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil

AIM：To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cis- platin on two cancer cell lines. METHODS： Colorectal carcinoma （HT29） and human hepatocellular carcinoma （HepG2） cells were treated with spray-dried extracts of Phyllanthus niruri （SDEPN） either alone or in combination with cisplatin at differ- ent concentrations （0.5 mg/mL and 1 mg/mL） for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with prop- idium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histo- grams. Significant differences between groups were determined using analysis of variance and Bonferroni＇s test, as indicated. A value of P 〈 0.05 was considered to be statistically significant. RESULTS： SDEPN had significantly different cyto- toxic effects on HT29 （2.81 4- 0.11 vs 3.51 4- 1.13, P 〉 0.05） and HepG2 （5.07± 0.3 vs 15.9 ± 1.04, P 〈 0.001） cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 （1.91 ± 0.57 vs 4.53± 1.22, P 〉 0.05） and HepG2 （14.56 ± 1.6 vs 35.67 ± 3.94, P 〈 0.001） cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells （HepG2 cells for 4 h： 10.78 ± 1.58 vs 53.89 ± 1.53, P 〈 0.001; 24 h： 8.9 ± 1.43 vs 62.78 ± 1.87, P 〈 0.001 and HT29 cells for 4 h： 9.52 ±0.913 vs 49.86 ± 2.89, P 〈 0.001; 24 h： 11.78 ± 1.05 vs 53.34 ± 2.65, P 〈 0.001）. In HT29 cells, pretreat- ment with SDEPN and subsequent treatment with cis-platin resulted in a greater number of cells being killed （12.78 ± 1.01 vs 93.76 ± 1.6, P 〈 0.001）. HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin （12.87 ± 2.78 vs 78.8 ± 3.02, P 〈 0.001）. CONCLUSION： SDEPN is selectively toxic ag

关键词： Cisplatin Colorectal cancer Liver cancer Phyllanthus n/ruri Cytotoxic effect

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How to improve colon cancer screening rates

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World Journal of Gastrointestinal Oncology 2015年第12期7卷 484-491页

作者： luiz Ronaldo Alberti Diego Paim Carvalho Garcia Debora Lucciola coelho David Correa Alves De Lima Andy Petroianu Department of Surgery Federal University of Minas Gerais Medical Sciences of Minas Gerais

Colorectal carcinoma is a common cause of death throughout the world and may be prevented by routine control, which can detect precancerous neoplasms and early cancers before they undergo malignant transformation or metastasis. Three strategies may improve colon cancer screening rates: convince the population about the importance of undergoing a screening test; achieve higher efficacy in standard screening tests and make them more available to the community and develop new more sensitive and efficacious screening methods and make them available as routine tests. In this light, the present study seeks to review these three means through which to increase colon cancer screening rates.

关键词： Colon cancer screening Colon cancer Screening test

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