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Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado Manuel García-Gutiérrez María luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero luis jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez luis Martín-Villén JoséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious Diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain Department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious Diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious Diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory Diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain Department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain Digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain Neurology and Clinical Neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and Nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and Nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity *** cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease *** this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory ***,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 ***,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were *** findings contribute to a better understanding of the immunological sequelae of COVID-19.

关键词： SARS-CoV-2 COVID-19 Dendritic cell Long-COVID

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Magnetocaloric effect in ErNi2 melt-spun ribbons

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Journal of Rare Earths 2020年第6期38卷 612-616页

作者： J.L.Sanchez Llamazares P.Ibarra-Gaytan C.F.Sanchez-Valdes D.Rlos-jara P.Alvarez-Alonso Instituto Potosino de Investigacion Ciendfica y Tecnoldgica A.C. Camino a la Presa San Jose 2055Col.Lomas 4San Luis Potosi.S.LP.78216Mexico CPM-TIP University Pavol Jozef SafarikPark Angelinum 9.04154 KosiceSlovakia Division Multidisciplinaria Ciudad UniversitariaUniversidad Aurdnoma de Ciudad Juarez(UACJ)Calle Jose de Jesus Macias Delgado#18100Ciudad Juarez 32579Chihuahua.Mexico Departamento de Fisica Universidad de OviedoCalvo Sotelo s/n33007 OviedoSpain

ErNi2 ribbons were produced by rapid solidification using the melt spinning *** structural,magnetic and magnetocaloric properties in the as-solidified state were studied by X-ray diffraction,scanning electron microscopy,magnetization and specific heat *** are single phase with the MgCu2-type crystal structure,a Curie temperature TC of 6.8 K and a saturation magnetization at2 K and 5 T of 124.0 A·m2/*** a magnetic field change μ0△H of 5 T(2 T) ribbons show a maximum magnetic entropy change |△SMpeak| of 24.1(16.9) J/(kg·K),and an adiabatic temperature change △Tadmax of8.1(4.4) K;this is similar to the previously reported literature for bulk alloys that were processed through conventional melting techniques followed by prolonged thermal *** addition,the samples also show slightly wider △SM(T) curves with respect to bulk alloys leading to a larger refrigerant capacity.

关键词： ErNi2 Laves phase Melt-spun ribbons Magnetic entropy change Adiabatic temperature change Rare Earths

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Anticardiolipin Antibody Isotype Determination in Amniotic Fluid of Pregnant Patients with Systemic Lupus Erythematosus and/or Antiphospholipid Syndrome

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Open Journal of Rheumatology and Autoimmune Diseases 2014年第3期4卷 170-177页

作者： Xibillé-Friedmann Daniel Sánchez-Rodríguez Carmen Cruz-Cruz Polita García de la Torre Ignacio jara-Quezada luis Javier Departamento de Perinatología Hospital de Ginecoobstetricia 3 Centro Médico Nacional La Raza Instituto Mexicano del Seguro Social México Distrito Federal México Departamento de Reumatología Hospital General de Cuernavaca “Dr. José G. Parres” Servicios de Salud de Morelos Cuernavaca Morelos México Ginecología y Obstetricia Torre Médica San Diego Cuernavaca Morelos México

Objective: To determine the levels and isotypes of aCl, as well as anti beta 2 glycoprotein 1 (antiβ2-GP1) antibodies in serum and amniotic fluid of pregnant patients with SLE and/or APLS, and healthy pregnant women serving as a control group. Material and Methods: We analyzed serum and amniotic fluid of pregnant patients with SLE and/or APLS, and of healthy pregnant women through ELISA. Results were compared using a Student’s T test. Results: 6 of 13 patients (46.1%), 5 with SLE and one with primary APLS had antiphospholipid antibodies in amniotic fluid. Two patients had IgG aCl and 4 patients had antiβ2-GP1 (one of them also showing IgM) in amniotic fluid. In serum, 4 patients (30%) had antiphospholipid antibodies present (one IgG aCl and three anti β2-GP1) as opposed to none in the control group having antiphospholipid antibodies in amniotic fluid. Only one control had IgM aCl in serum. Antiβ2-GP1 in the amniotic fluid of patients showed a statistically significant value when compared to controls. Conclusion: aCl and antiβ2-GP1 may be present in the amniotic fluid of patients with and without a history of fetal loss. The presence of IgM aCl and antiβ2-GP1 in amniotic fluid suggests its localized production.

关键词： Antiphospholipid Syndrome Systemic Lupus Erythematosus Pregnancy Amniotic Fluid

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Intravitreal Bevacizumab for the Treatment of Chronic or Recurrent Central Serous Chorioretinopathy

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Open Journal of Ophthalmology 2014年第3期4卷 57-64页

作者： Mitzy E. Torres Soriano Gerardo García Aguirre Maximiliano Gordon Angelozzi Veronica Kon jara Jans Fromow Guerra Michael D. Ober Hugo Quiroz Mercado Centro de la Visión Gordon Manavella Rosario Argentina Department of Ophthalmology University of North Carolina Chapel Hill USA Ophthalmology Service at Denver Health Medical Center Denver USA Retina Consultants of Michigan Southfield USA Retina Department Asociación para evitar la Ceguera Hos-pital Dr. Luis Sanchez Bulnes Mexico City Mexico Unidad Oftalmológica “Dr Torres López” Centro Médico Cagua Cagua Venezuela

Background: We report the use of intravitreal bevacizumab as an option for the treatment of chronic or recurrent central serous chorioretinopathy (CSC). Methods: Eight eyes with chronic or recurrent CSC received intravitreal bevacizumab (1.25 mg/0.05 cc) and underwent best corrected visual acuity (VA), optical coherent tomography (OCT), fluorescein angiography (FA) and indocyanine green angiography (ICG) before, and one, three and six months after treatment. Results: All patients showed improvement in visual acuity, fluorescein angiographic leakage, choroidal hyperpermeability and reduced or resolved neurosensory detachment following treatment. Two patients require a second dose of intravitreal bevacizumab. Conclusions: Intravitreal injection of bevacizumab was associated with visual improvement and reduced neurosensory detachment without adverse events in patients with chronic or recurrent CSC. Although these results are promising, further investigations would be helpful to understand this therapy for patients with CSC.

关键词： Central Serous Chorioretinopathy Intravitreal Bevacizumab Retinal Pigment Epithelium Detachment

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Effect of Polishing Procedure on the Roughness and Bacterial Adhesion of Provisional Restorative Materials

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Microscopy Research 2018年第2期6卷 9-18页

作者： Néstor Usiel Lara-jara Gabriel Fernando Romo-Ramírez María del Pilar Goldaracena-Azuara Antonio Aragón-Piñ a Claudia Butrón-Téllez Girón María Verónica Méndez-González Ana María González-Amaro Faculty of Stomatology Autonomous University of San Luis Potosí San Luis Potosí City México Faculty of Engineering Institute of Metallurgy Autonomous University of San Luis Potosí San Luis Potosí City México Laboratory of Biochemistry Microbiology and Pathology Faculty of Stomatology Autonomous University of San Luis Potosí San Luis Potosí City México

Provisional prosthetic restoration materials are exposed to oral cavity producing on the surface biofilm where different factors such as surface roughness and porosity can condition their formation and organization and can create stagnation areas that promote the bonding of organic particles, thus facilitating the formation and maturation of the biofilm. The purpose of this study was to compare surface roughness of two provisional prosthetic restorations materials and their bacterial susceptibility. In this study, two provisional restoration materials were used in two groups, A polymethyl methacrylate acrylic (NicTone MDC DENTAL) and B bis-acryl resin (Protemp 4 ESPE 3M). A total of 80 samples (40 samples of each material) were in thick plates of 10 × 10 mm and 2 mm high. 20 samples of each material were polished, while 20 were left unpolished. Subsequently, the samples were observed by Atomic Force Microscopy for their evaluation of surface roughness. The values were analyzed with t-test, Mann-Whitney U test and Kruskal Wallis test. The samples were microbiologically inoculated with the strains obtained and identified from a provisional polymethyl methacrylate acrylic restoration in a patient, in order to observe bacterial adhesion using a Scanning Electron Microscope. Two strains, Enterococcus faecalis and Pseudomona luteola, were identified. The presence of the microorganisms was observed on the surface of both materials, either polished or unpolished, with a lower level of microorganism adhesion found on the bis-acrylic resin. There was a significant difference about surface roughness in the groups A and B with p -8). We found that the polished bis-acrylic resin showed lower surface roughness and bacterial adhesion in comparison with the polymethyl methacrylate.

关键词： Surface Analysis Bacterial Adhesion Provisional Fixed Restoration

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