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Expression Patterns of CAPN1 and CAPN8b Genes during Embryogenesis in Xenopus laevis

CellBio 2013年第4期2卷 211-216页

作者： lucie abrouk-vérot Claire Brun Jean-Marie Exbrayat Université de Lyon UMRS 449 Biologie Générale Université Catholique de Lyon Reproduction et Développement Comparé Ecole Pratique des Hautes Etudes Lyon France

Calpains are a superfamily of Ca2+-dependent cysteine proteases, implicated in various cellular processes and thus probably necessary in all the stages of cell life. The first extended report of quantification of total RNAs within the developmental stages of Xenopus laevis was described in this study. Decreases of total RNAs were positively associated with waves of apoptotic cell death (onset of gastrulation, and morphogenesis). Using qPCR, the temporal expression pattern of CAPN1 and CPAN8b (XCL-2) were characterized during the Xenopus laevis embryogenesis. Transcripts of the CAPN1 and CAPN8 genes were detectable from gastrula stage and their levels oscillated throughout development. The expression of the CAPN1 (mu/I) gene was observed in earliest stage, indicating a maternal origin, while expression of the CAPN8b gene was detectable after midblastula transition. The levels of the two transcripts then started to rise again obviously as a result of zygotic expression (stage 11). The CAPN1 gene expression was particularly expressed at tailbud stage, while the CAPN8 transcripts were found at gastrula, neurula and tailbud stages. This is the first report of quantification of mRNAs CAPN8b and CAPN1 (mu/I) within the developmental stages of Xenopus laevis by qPCR.

关键词： Calpain Embryonic Development Cell Death Xenopus laevis

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Apoptosis in amphibian development

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Advances in Bioscience and Biotechnology 2012年第6期3卷 669-678页

作者： Jean-Marie Exbrayat Elara N. Moudilou lucie abrouk Claire Brun Université de Lyon UMRS 449 Biologie générale Université catholique de Lyon Reproduction et développement comparé Ecole Pratique des Hautes Etudes 25 rue du Plat F-692088 Lyon Cedex 02 France

Amphibians and more particularly X. laevis are models often used for studying apoptosis during embryonic development. Using several methods, searchers determined the localization of programmed cell deaths (PCD). Several experimental methods also have been used to understand the regulatory mechanisms of apoptosis, throughout development, contributing to elucidate the general action of several genes and proteins. Apoptosis occurs very early, with a first program under control of maternal genes expressed before MBT, in order to eliminate damaged cells before gastrulation, and a second program at the onset of gastrulation. PCD is also observed during neurulation. Then, apoptotic cells are observed in amphibian organogenesis and metamorphosis. Results of these researches showed both importance of PCD for embryonic development, and the complexity of its regulation. Results obtained can be useful to understand others aspects of the importance of apoptosis, particularly pathological aspects.

关键词： PCD Apoptosis Development Metamorphosis Amphibian Xenopus

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