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Polydopamine-coated photoautotrophic bacteria for improving extracellular electron transfer in living photovoltaics

Nano Research 2024年第2期17卷 866-874页

作者： Melania Reggente Charlotte Roullier Mohammed Mouhib Patricia Brandl Hanxuan Wang Stefano Tacconi Francesco Mura luciana dini Rossella Labarile Massimo Trotta Fabian Fischer Ardemis A.Boghossian Institute of Chemical Sciences and Engineering(ISIC) Ecole Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Department of Biology and Biotechnology Charles Darwin Sapienza University of RomeRome 00185Italy Research Center on Nanotechnology Applied for Engineering of Sapienza(CNIS) University of Rome SapienzaRome 00185Italy CNR-IPCF Consiglio Nazionale delle Ricerche–Istituto per i Processi Chimico Fisici Bari 70126Italy Institute of Life Technologies HES-SO Valais-WallisSion 1950Switzerland Institute for Renewable Energy HES-SO Valais-WallisSion 1950Switzerland

Living photovoltaics are microbial electrochemical devices that use whole cell–electrode interactions to convert solar energy to *** bottleneck in these technologies is the limited electron transfer between the microbe and the electrode *** study focuses on enhancing this transfer by engineering a polydopamine(PDA)coating on the outer membrane of the photosynthetic microbe Synechocystis *** coating provides a conductive nanoparticle shell to increase electrode adhesion and improve microbial charge extraction.A combination of scanning electron microscopy(SEM),transmission electron microscopy(TEM),UV–Vis absorption,and Raman spectroscopy measurements were used to characterize the nanoparticle shell under various synthesis *** cell viability and activity were further assessed through oxygen evolution,growth curve,and confocal fluorescence microscopy *** results show sustained cell growth and detectable PDA surface coverage under slightly alkaline conditions(pH 7.5)and at low initial dopamine(DA)concentrations(1 mM).The exoelectrogenicity of the cells prepared under these conditions was also characterized through cyclic voltammetry(CV)and chronoamperometry(CA).The measurements show a three-fold enhancement in the photocurrent at an applied bias of 0.3 V(***/AgCl[3 M KCl])compared to non-coated *** study thus lays the framework for engineering the next generation of living photovoltaics with improved performances using biosynthetic electrodes.

关键词： biophotovoltaics bioelectronics photosynthetic bacteria cyanobacteria polydopamine adherence

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Wnt signaling pathway inhibitor promotes mesenchymal stem cells differentiation into cardiac progenitor cells in vitro and improves cardiomyopathy in vivo

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世界干细胞杂志:英文版(电子版) 2023年第8期15卷 821-841页

作者： Rabbia Muneer Rida-e-Maria Qazi Abiha Fatima Waqas Ahmad Asmat Salim luciana dini Irfan Khan Dr.Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological SciencesUniversity of KarachiKarachi 75270SindhPakistan Department of Biology and Biotechnologies Charles Darwin Sapienza University of RomeRome 00185Italy

BACKGROUND Cardiovascular diseases particularly myocardial infarction(MI)are the leading cause of mortality and morbidity around the *** cardiac tissue possesses very limited regeneration potential,therefore use of a potent small molecule,inhibitor Wnt production-4(IWP-4)for stem cell differentiation into cardiomyocytes could be a promising approach for cardiac *** pathway inhibitors may help stem cells in their fate determination towards cardiomyogenic lineage and provide better homing and survival of cells in *** stem cells(MSCs)derived from the human umbilical cord have the potential to regenerate cardiac tissue,as they are easy to isolate and possess multilineage differentiation ***-4 may promote the differentiation of MSCs into the cardiac *** To evaluate the cardiac differentiation ability of IWP-4 and its subsequent in vivo *** Umbilical cord tissue of human origin was utilized to isolate the MSCs which were characterized by their morphology,immunophenotyping of surface markers specific to MSCs,as well as by tri-lineage differentiation *** analysis was performed to identify the optimal concentration of *** were treated with 5μM IWP-4 at two different time *** of MSCs into cardiomyocytes was evaluated at DNA and protein *** MI ratmodel was ***-4 treated as well as untreated MSCs were implanted in the MI model,then the cardiac function was analyzed via *** were labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate(DiI)dye for tracking,while the regeneration of infarcted myocardium was examined by histology and *** MSCs were isolated and *** analysis showed that IWP-4 was non-cytotoxic at 5μM *** specific gene and protein expression analyses exhibited more remarkable results in fourteen days treated group that was eventually sel

关键词： Myocardial infarction Inhibitor Wnt production-4 Differentiation Mesenchymal stem cells Wnt pathway Cardiac function

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Advances in Active Suspension Systems for Road Vehicles

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Engineering 2024年第2期33卷 160-177页

作者： Min Yu Simos AEvangelou Daniele dini Department of Mechanical Engineering Imperial College LondonLondon SW72AZUK Department of Electrical and Electronic Engineering Imperial College LondonLondon SW72AZUK

Active suspension systems(ASSs)have been proposed and developed for a few decades,and have now once again become a thriving topic in both academia and industry,due to the high demand for driving comfort and safety and the compatibility of ASSs with vehicle electrification and *** review papers on ASSs mainly cover dynamics modeling and robust control;however,the gap between academic research outcomes and industrial application requirements has not yet been bridged,hindering most ASS research knowledge from being transferred to vehicle *** paper comprehensively reviews advances in ASSs for road vehicles,with a focus on hardware structures and control *** particular,state-of-the-art ASSs that have been recently adopted in production cars are discussed in detail,including the representative solutions of Mercedes active body control(ABC)and Audi predictive active suspension;novel concepts that could become alternative candidates are also introduced,including series active variable geometry suspension,and the active wheel-alignment *** with compact structure,small mass increment,low power consumption,high-frequency response,acceptable economic costs,and high reliability are more likely to be adopted by car *** terms of control strategies,the development of future ASSs aims not only to stabilize the chassis attitude and attenuate the chassis vibration,but also to enable ASSs to cooperate with other modules(e.g.,steering and braking)and sensors(e.g.,cameras)within a car,and even with high-level decision-making(e.g.,reference driving speed)in the overall transportation system-strategies that will be compatible with the rapidly developing electric and autonomous vehicles.

关键词： Active suspension Vehicle dynamics Robust control Ride comfort Chassis attitude

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The effects of mixed prebiotics in aquaculture:A review

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Aquaculture and Fisheries 2024年第1期9卷 28-34页

作者： Wendy Wee Noor Khalidah Abdul Hamid Khairiyah Mat Raja Ili Airina Raja Khalif Nor dini Rusli Mohammad Mijanur Rahman Muhammad Anamul Kabir Lee Seong Wei Centre for Foundation and Continuing Education Universiti Malaysia Terengganu21030Kuala NerusTerengganuMalaysia School of Biological Sciences Universiti Sains Malaysia11800MindenPulau PinangMalaysia Department of Agricultural Sciences Faculty of Agro-Based IndustryUniversiti Malaysia KelantanJeli Campus17600JeliKelantanMalaysia Faculty of Fisheries Sylhet Agricultural UniversitySylhet3100Bangladesh

Aquaculture industry contributes significant aquaculture species production to meet fish protein demand from the *** industry is gearing towards intensification to increase more production in the near *** technologies and efforts were introduced into aquaculture industry to bring it up to the next higher ***,the development of aquaculture industry is hindered by disease *** a result,excessive use of antibiotics and chemicals were applied to reduce or minimize risk of disease outbreak in aquaculture farming ***,aquacultures products and environment were highly exposed to these antibiotics and *** resolve this issue,fish farmers were encouraged to utilize alternative method in aquaculture species health *** has become a new approach in aquaculture industry to maintain aquaculture species health and a good environment at the same time produce chemical and antibiotic-free aquaculture *** this review paper,we summarize and discuss the effects of prebiotic mixtures administration on growth performance,immune response,disease resistance,and tolerance against abiotic stressors of aquatic animals.

关键词： Growth performance Immunosaccharide Disease resistance Stress relieve

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转抗虫耐除草剂基因玉米对根际细菌群落结构和功能的影响

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环境科学研究 2024年第10期37卷 2171-2180页

作者：郑怀谷胡刚于赐刚章嫡妮王琛张振华浙江大学地球科学学院浙江杭州310058 生态环境部南京环境科学研究所江苏南京210042 中国药科大学工学院江苏南京211198

抗虫耐除草剂是转基因玉米的最主要性状,根际土壤细菌是转基因植物生态风险评估中的重要监测对象,目前关于转抗虫耐除草剂基因对玉米根际土壤细菌群落结构和功能的影响尚不清楚。本研究以华兴单和罗单2个转基因玉米品种及其亲本为研究对... 详细信息

抗虫耐除草剂是转基因玉米的最主要性状,根际土壤细菌是转基因植物生态风险评估中的重要监测对象,目前关于转抗虫耐除草剂基因对玉米根际土壤细菌群落结构和功能的影响尚不清楚。本研究以华兴单和罗单2个转基因玉米品种及其亲本为研究对象,采用高通量测序技术并结合PICRUSt功能预测,分析转基因对玉米根际细菌群落结构和功能的影响。结果表明:①转基因对细菌主要门和属的组成及丰度没有显著影响,但对硝化螺菌门、鞘氨醇单胞菌属和硝化螺菌属等丰度有显著影响。②冗余分析表明,土壤pH、电导率及速效磷对细菌群落结构具有显著影响,但转基因的影响并不显著。③PICRUSt功能预测结果表明,转基因对31种二级功能没有显著影响,对氮磷循环功能影响也不明显;而玉米品种和生长阶段对25种二级功能具有显著影响,而且品种也是影响氮磷循环相关功能的重要因素。研究显示,转基因对玉米根际细菌群落结构及功能并无显著影响,而玉米品种和生长阶段的影响则更为显著。

关键词：转抗虫耐除草玉米品种根际细菌群落 PICRUSt 氮磷循环

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OsSCE1 Encoding SUMO E2-Conjugating Enzyme Involves in Drought Stress Response of Oryza sativa

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Rice science 2018年第2期25卷 73-81页

作者： dini NURDIANI Dwi WIDYAJAYANTIE Satya NUGROHO Laboratory of Genomics and Crop Improvement Research Center for Biotechnology Indonesian Institute of Sciences

Small ubiquitin-like modifier(SUMO)-conjugating enzymes are involved in post-translational regulatory processes in eukaryotes, including the conjugation of SUMO peptides to protein substrate(SUMOylation). SUMOylation plays an important role in improving plant tolerance to abiotic stress such as salt, drought, heat and cold. Herein, we reported the isolation of OsSCE1(LOC_Os10 g39120) gene encoding a SUMO-conjugating enzyme from rice(Oryza sativa cv. Nipponbare) and its functional validation in response to drought stress. The E2 enzyme, Os SCE1, is one of three key enzymes involved in the conjugation of SUMO to its target proteins. Activated SUMO is transferred to the cysteine of an E2 enzyme and then to the target lysine residue of the substrate, with or without the help of an E3 SUMO ligase. Expression of OsSCE1 was strongly induced by polyethylene glycol 6000(PEG6000) treatment, which suggested OsSCE1 may be involved in the drought stress response. Overexpression of OsSCE1(OsSCE1-OX) in Nipponbare reduced the tolerance to drought stress. Conversely, the drought tolerance was slightly improved by the knockdown of OsSCE1(OsSCE1-KD). These results were further supported by measurement of proline content in OsSCE1-OX and OsSCE1-KD transgenic lines under induced drought stress, which showed OsSCE1-KD transgenic lines accumulated higher proline content than the wild type, whereas OsSCE1-OX line had lower proline content than the wild type. These findings suggested OsSCE1 may play a role as a negative regulator in response to drought stress in rice.

关键词： Oryza sativa drought stress small ubiquitin-like modifier SUMO-conjugating enzyme proline content gene expression

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A unified electrical model based on experimental data to describe electrical transport in carbon nanotube-based materials

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Nano Research 2020年第6期13卷 1764-1779页

作者： Yoann dini Jérôme Faure-Vincent Jean Dijon Univ.Grenoble-Alpes CEALITENDTNMF-38000GrenobleFrance Univ.Grenoble-Alpes CEACNRSIRIGSyMMESF-38000GrenobleFrance

Understanding the electrical transport in carbon nanotube(CNT)materials is one key to reach very high electrical *** CNT material resistivity(ρ(T))as function of the temperature are fully apprehended by their reduced activation W(T)=dln(ρ)/dln(T)*** to now,no model accurately fits W(T)curves,thus preventing from precisely describing the CNT material electrical *** present a new electrical transport model that perfectly fits all W(T)curves found in the literature and in our own *** material resistivities are modeled byρ(T)=ρ0(T^−α+M(1+βTγT^2)).Our model has few enough parameters(α,M,β,γ)to relate them to the CNT *** 70 K,we experimentally show that CNT material resistivity follows the Luttinger Liquid theory justifying the T^−αterm in our *** 70 K,the polynomial part becomes dominant and depends on the two different CNT fabrication techniques which lead to two very different yarn *** yarns made from floating catalyst chemical vapor deposition CNTs,the polynomial is explained by the percolation of metallic CNT ***,the polynomial of yarns spun from CNT arrays is explained by the electrical transport in CNT bundles which are the basic building blocks of this type of yarns.

关键词： carbon nanotube carbon-based materials electrical conductivity electrical transport model Luttinger Liquid

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On the origin of plasticity-induced microstructure change under sliding contacts

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Friction 2023年第3期11卷 473-488页

作者： Yilun XU Daniel S.BALINT Christian GREINER Daniele dini Department of Mechanical Engineering Imperial College LondonLondon SW72AZUK Department of Materials Imperial College LondonLondon SW72AZUK Karlsruhe Institute of Technology(KIT) Institue for Applied Materials(IAM)Kaisertrasse 12Karlsruhe 76131Germany KITIAM-CMS MicroTribology Center(μTC) Strasse am Forum 5Karlsruhe 76131Germany

Discrete dislocation plasticity(DDP)calculations are carried out to investigate the response of a single crystal contacted by a rigid sinusoidal asperity under sliding loading conditions to look for causes of microstructure change in the dislocation *** mechanistic driver is identified as the development of lattice rotations and stored energy in the subsurface,which can be quantitatively correlated to recent tribological experimental *** of surface slip initiation and substrate permanent deformation obtained from DDP calculations for varying contact size and normal load suggest ways of optimally tailoring the interface and microstructural material properties for various frictional loads.

关键词： discrete dislocation plasticity sliding size effect microstructure change

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Thermo-magnetic analysis of thick-walled spherical pressure vessels made of functionally graded materials

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Applied Mathematics and Mechanics(English Edition) 2019年第6期40卷 751-766页

作者： M. A. NEMATOLLAHI A. dini M. HOSSEINI Department of Biosystems Engineering College of AgricultureShiraz UniversityShiraz 71441-65186Iran Department of Mechanical Engineering Ferdowsi University of MashhadMashhad 91775-1111Iran Department of Mechanical Engineering Sirjan University of TechnologySirjan 78137-33385Iran

This study presents an analytical solution of thermal and mechanical displacements, strains, and stresses for a thick-walled rotating spherical pressure vessel made of functionally graded materials (FGMs). The pressure vessel is subject to axisymmetric mechanical and thermal loadings within a uniform magnetic field. The material properties of the FGM are considered as the power-law distribution along the thickness. Navier’s equation, which is a second-order ordinary differential equation, is derived from the mechanical equilibrium equation with the consideration of the thermal stresses and the Lorentz force resulting from the magnetic field. The distributions of the displacement, strains, and stresses are determined by the exact solution to Navier’s equation. Numerical results clarify the influence of the thermal loading, magnetic field, non-homogeneity constant, internal pressure, and angular velocity on the magneto-thermo-elastic response of the functionally graded spherical vessel. It is observed that these parameters have remarkable effects on the distributions of radial displacement, radial and circumferential strains, and radial and circumferential stresses.

关键词： analytical solution magnetic field thermal loading rotating thick-walled spherical pressure vessel functionally graded material (FGM)

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Seismic assessment of nature-inspired hexagrid lateral load-resisting system

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Earthquake Engineering and Engineering Vibration 2021年第3期20卷 661-672页

作者： Yasaman Memarzadeh Kiani Seyed Hossein Hosseini Lavassani Afshin Meshkat-dini Department of Civil Engineering Faculty of EngineeringKharazmi UniversityTehranIran

This paper provides a general perspective of the seismic performance of a nature-inspired,honey-comb grid structural system,known as a hexagrid,under near-field ground *** performance of this skeleton is then compared to that of a bundled-tube,as a conventional and efficient load-resisting system in order to provide a better perception of the seismic behavior of a hexagrid *** 20-story buildings with bundled-tube and hexagrid skeleton were *** behavior of the structures was investigated through 3-D finite element computer models and nonlinear time history analyses by subjecting the models to seven three-component records of scaled near-field ground *** of peak inter story drift and corner beam-column joint rotations were calculated and *** indicated that by replacing the exterior columns of the bundled-tube system with inclined beam-column elements of nature-inspired hexagons,lateral stiffness of the building increased and it would tolerate less deformations before global dynamic instability is *** presence of inclined columns in the hexagrid skeleton helped to concentrate local nonlinearities in ring beams rather than exterior columns.

关键词： high-rise steel structure hexagrid structure performance assessment nonlinear seismic analysis

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