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Spatiotemporal oxygen dynamics in young leaves reveal cyclic hypoxia in plants

Molecular Plant 2024年第3期17卷 377-394页

作者： Paolo M.Triozzi luca brunello Giacomo Novi Gianmarco Ferri Francesco Cardarelli Elena Loreti Mariano Perales Pierdomenico Perata PlantLab Center of Plant SciencesSant'Anna School of Advanced Studies56010 PisaItaly Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid(UPM)-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria(INIA-CSIC)Campus de Montegancedo UPMPozuelo de Alarcon28223 MadridSpain Fondazione Pisana per la Scienza 56017 PisaItaly Laboratorio NEST Scuola Normale SuperioreIstituto Nanoscienze-CNRPiazza S.Silvestro1256127 PisaItaly institute of Agricultural Biology and Biotechnology National Research Council56124 PisaItaly Departamento de Biotecnologia-Biologia Vegetal Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de BiosistemasUniversidad Politecnica de Madrid(UPM)28040 Madrid.Spain

Oxygen is essential for plant growth and *** occurs in plants due to limited oxygen avail-abilityfollowing adverse environmental conditions as well inhypoxic niches in otherwisenormoxic ***,the existence and functional integration of spatiotemporal oxygen dynamics with plant development remains *** animal systems dynamic fluctuations in oxygen availability are known as cyclic *** this study,we demonstrate that cyclic fluctuations in internal oxygen levels occur in young emerging leaves of Arabidopsis *** hypoxia in plants is based on a mechanism requiring the ETHYLENE RESPONSE FACTORS type VII(ERFVII)that are central components of the oxygen-sensing machinery in *** ERFVll-dependent mechanism allows precise adjustment of leaf growth in response to carbon status and oxygen availability within plant *** study thus establishes a functional connection between internal spatiotemporal oxygen dynamics and developmental processes of plants.

关键词： Arabidopsis hypoxia cyclic hypoxia leaf development oxygen-sensing mechanism ERFVII

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CO2 adsorption in a confined fluidized bed of zeolite pellets: Influence of operating velocity

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Particuology 2019年第5期17卷 67-74页

作者： Rossella Girimonte brunello Formisani Flaviano Testa Diparcimento di Ingegneria Informatica Modellistica Elettronica e Sistemistica Universita della Calabria 187030 Arcavacata di Rende Cosenza Italy

CO2 adsorption is performed across a fluidized bed comprising a commercial pelletized 13X zeolite con-fined to the interstitial void network of a coarse glass sphere packed bed. Compared with traditional fixed bed adsorption, the packed fluidized system allows operation across a wide-range of gas velocities without a substantial increase in pressure drop. Additionally, with respect to conventional fluidization regimes, the technique adopted herein prevents the formation of bubbles in favour of enhancing the bed expansion ability. Furthermore, for a given mass of sorbent, the C02 uptake capacity is observed to increase as a result of improved thermal conditions and in eliminating any by-pass effect at the gas-particle interface, which is associated to the suppression of bubbling.

关键词： CO2 capture Adsorption Zeolite 13X Confined fluidized beds Packed fluidized bed

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NEM^® Brand Eggshell Membrane Effective in the Treatment of Pain and Stiffness Associated with Osteoarthritis of the Knee in an Italian Study Population

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International Journal of Clinical Medicine 2016年第2期7卷 169-175页

作者： Emy brunello Antonio Masini Health Department “REGENESIS”Medical Center Bovolone Italy Complementary Medicine R & D Phytonaturesas Bovolone Italy

A single-center, open-label clinical study was conducted to evaluate the efficacy and safety of NEM® as a natural treatment for pain and stiffness associated with osteoarthritis of the knee in an Italian population. NEM® brand eggshell membrane is a unique dietary supplement that contains naturally occurring glycosaminoglycans and proteins essential for maintaining healthy joints and connective tissues. Twenty-five subjects received oral NEM®, 500 mg once daily for four weeks. The primary outcome measure was to evaluate the mean effectiveness of NEM® in relieving general pain associated with moderate osteoarthritis of the knee at 10 and 30 days utilizing a 10-question, abbreviated questionnaire based on the WOMAC osteoarthritis questionnaire. Supplementation with NEM® produced a significant treatment response from baseline at both 10 days and 30 days for composite pain (40.6% reduction, p p p = 0.009;59.7% reduction, p ® is an effective and safe natural therapeutic option for the treatment of both pain and stiffness associated with osteoarthritis of the knee. Supplementation with NEM®, 500 mg taken once daily, significantly reduced both pain and stiffness rapidly (10 days) and this effect continued to improve through 30 days. There was also a meaningful reduction in the amount of analgesic consumed on a weekly basis, which further enhanced patients’ safety.

关键词： Knee Osteoarthritis Supplement Egg Shell Membrane Glycosaminoglycans

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Novel Solid State Nitric Oxide Sensor Using Siloxane-Poly(Oxypropylene)(PPO)

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Materials Sciences and Applications 2013年第11期4卷 683-688页

作者： Rondinelli D.Herculano Carlos A.brunello Jair P.Melo Jr Mayler Martins Felipe ABorges Leila A.Chiavacci Carlos F.O.Graeff Departamento de Ciências Biológicas Faculdade de Ciencias e Letras de AssisUniversidade Estadual Paulista“Júlio de Mesquita Filho”AssisBrazil Departamento de Física Faculdade de FilosofiaCiências e Letras de Ribeirao PretoUniversidade de Sao PauloRibeirão PretoBrazil Departamento de Física Faculdade de CiênciasUniversidade Estadual Paulista“Júlio de Mesquita Filho”BauruBrazil Departamento de Fármacos e Medicamentos Faculdade de Ciências FarmacêuticasUniversidade Estadual Paulista“Júlio de Mesquita Filho”AraraquaraBrazil

In this paper, a novel solid state Nitric Oxide (NO) sensor made of a spin trap (iron(II)-diethyldithiocarbamate complex, FeDETC) encapsulated in a siloxane-poly(oxypropylene) (PPO) matrix was developed. Nitric oxide (NO), a free radical molecule, has numerous roles in various physiological functions, such as the regulation of blood pressure, immune response to bacterial infection, and nervous systems. Siloxane-polyether hybrid materials, for example siloxane-poly(oxypropylene) (PPO), are easy to prepare, transparent and flexible. The combination of all these characteristics in a unique material allows it to be used in several scientific and technological areas, including human health. NO radical is trapped in FeDETC, which allows its detection by electron paramagnetic resonance (EPR). FeDETC was added while PPO was a sol, which was then left in air for gelation. The novel sensor was dived directly into a solution of NO, when the NO-FeDETC complex was formed. Our results show that the novel sensor responds to NO, with similar sensitivity as previously published sensors. PPO sensors present a strong EPR signal and a high stability, keeping its signal for 45 days. We have studied ways to accelerate the NO release from the sensor, in order to study its potential as a drug delivery system. We observed an acceleration in NO release by using a modulated magnetic field of 40 G at 100 kHz;as well as by UV irradiation. Thermal induced NO release was also tested by heating NO-FeDETC PPO up to 50°C, with good results.

关键词： Biomaterials Nitric Oxide PPO Drug Delivery System

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Profile of People Living with HIV/AIDS in a Large Municipality of Sao Paulo State, Brazil （2012-2013）

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Journal of Health Science 2014年第2期2卷 94-101页

作者： Gabriela Tavares Magnabosco Livia Maria Lopes Mayara Falico Faria Maria Eugenia Firmino brunello Tiemi Arakawa Aline Araujo Antunes Rubia Laine de Paula Andrade Aline Aparecida Monroe Tereza Cristina Scatena Villa College of Nursing of Ribeirao Preto University of Sao Paulo Ribeirao Preto 14040-902 Sao Paulo Brazil

HIV/AIDS has brought to light the challenge of incorporating the many influences between living conditions, social characteristics and health services performance to an adequate care for PLWHA （people living with AIDS）. Vulnerability of these populations is under the responsibility of specialized care units whose assistance does not always occur according to their real needs and demands. Therefore, this study aimed to analyze demographic, social and clinical profiles of PLWHA, as well as their follow-up in SS （Specialized Health Services） in Ribeir^o Preto, Brazil. It is a descriptive study conducted by the application of structured questionnaires to 253 patients with HIV/AIDS in follow-up during the years of 2012-2013. Variables were analyzed by descriptive statistics procedures. The findings pointed out gender parity, aging population, low education and economic predominance of class C. Regarding clinical characteristics, there was a predominance of asymptomatic individuals, with no clinical manifestations of AIDS or major comorbidities. The main mode of transmission was through sexual contact. The results led to the need of adequating the assistance provided to the specificities inherent to PLWHA. The care provision should cross an interdisciplinary perspective, targeting recognition of problems and ensuring comprehensive health care adequate to users＇ needs and demands.

关键词： AIDS user profile evaluation of health services comprehensive health care.

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Study of the Firing Temperature of Marajoara Archaeological Ceramics by EPR Associated to INAA Data

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Journal of Environmental Science and Engineering(A) 2012年第9期1卷 1108-1116页

作者： Kelly P. Nunes Casimiro S. Munita Rosimeiri G. Toyota Paulo T.M. Oliveira Nilo F. Cano Denise Schaan Oswaldo Baffa Luiz C. Oliveira Carlos A. brunello Eduardo G. Neves Ce1ia C. Demartini Nuclear and Energy Research Institute University of Sao Paulo Sao Paulo 5508-000 Brazil Department of Anthropology Basic Campus 1 Federal University of Par6 Beldm 66075-110 Brazil Magnetic Resonance Laboratory Department of Physics University of Sao Paulo Ribeirao Preto 14040-901 Brazil Archaeology and Ethnology Museum Department of Archaeology University of Sao Paulo Sao Paulo 05508-900 Brazil

The Marajó Island is the largest island of the Marajō archipelago, located in the north of Brazil. In this region, at the end of 19th century, it was identified an archaeological society named Marajora, which occupied the island from 400 to 1,350 AD. It was characterized by a ceramic style recognized by its beauty and exuberance. Until this moment, the physical and chemical analysis studies in this ceramics are scarce and this kind of studies may help archaeological studies performed in the island. With this purpose, this work presents a preliminary study of the firing temperature in the ceramics. For that, the elementary concentrations of 109 samples from Museu de Archaeology and Ethnology Museum of the University of Sáo Paulo and samples collected in the Marajá Island were studied. The samples were analyzed using instrumental neutron activation analysis in which the elemental concentration of Ce, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Na, Nd, Rb, Sc, Sm, Ta, Tb, Th, U, Yb and Zn were determined. The interpretation of the results was made using multivariate statistical approaches and showed the existence of three chemical groups. Some samples of each cluster were chosen for the determination of their firing temperatures by electron paramagnetic resonance, in order to verify if different firing temperatures was associated with different elemental groups of ceramic samples. The results showed that there are no statistical differences in the firing temperature between the samples from Archaeology and Ethnology Museum and the contemporary samples of the Marajá Island.

关键词： Maraj6 island archaeological ceramics INAA EPR multivariate statistical analysis.

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