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Efficient and stable genera-tion of transgenic silkworm, Bombyx mori with the lepi-dopteran derived transposon piggyBac

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Chinese Science Bulletin 2005年第15期50卷 1617-1621页

作者： DAI Hongjiu XU Guojiang Thomas Jean-luc WANG Zhugang JIANG Rongjing FEI Jian Graduate School of Chinese Academy of Sciences Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200032 China Shanghai Research Center for Model Organism Shanghai 201203China Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Model Organism Division e-Institutes of Shanghai UniversitiesShanghai 200025 China Unité Nationale Séricicole/INRA 25 Quai J. J. Rousseau 69350 la Mulatière France thomas@univ-lyonl.fr.

The piggyBac transposable element was suc-cessfully used for generation of the transgenic silkworm, Bombyx mori. The EGFP was adopted in this study as a screening marker in transgenic vector under the control of an artificial promoter containing Pax-6 binding sites that can drive eye-specific genes expression in various insect species including B. mori and Drosophila. 111 independent trans-genic lines were obtained among 700 fertile G0 moths. Most of the transgenic lines contained two or more chromosomal insertions of the EGFP marker, which were stably inherited over more than six generations during the time of this pro-ject. PiggyBac-mediated transposition was confirmed by identifying the characteristic TTAA duplication sequence at the insertion sites. The stable and high efficient transmission of a genetic marker into B. mori confirms the usage of this vector-marker system for industrial production and theo-retical research.

关键词：小猪遗传基因基因转换基因表达动物实验

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Effects of aqueous extract from red liriope platyphylla on phthalic-anhydride-induced atopic dermatitis in Interleukin-4/luciferase/Consensus non-coding sequence-1 transgenic mice evaluated in terms of luciferase signal and general phenotype biomarkers

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Journal of Traditional Chinese Medicine 2017年第4期37卷 475-485页

作者： Moon Hwa Kwak Ji Eun Kim Jun go Hong Joo Son Hee Seob lee Jin Tae Hong Dae Youn Hwang College of Natural Resources and life Science/life and Industry Convergence Research Institute Pusan National UniversityMiryang 627-706South of Korea College of Human Ecology Pusan National UniversityBusan 609-735south of Korea College of Pharmacy and Medical Research Center Chungbuk National UniversityChungju 361-763south of Korea College of Pharmacology Chungbuk National UniversityChungju 361-763south of Korea

OBJECTIVE: To quantitatively evaluate the therapeutic effects of Red liriope platyphylla(RlP) on atopic dermatitis(AD), alterations in the luciferase(luc) signal and general phenotype biomarkers were compared in phthalic anhydride(PA) treated Interleukin-4(Il-4)/luc/Consensus non-coding sequence-1(CNS-1) transgenic(Tg) mice following treatment with aqueous extract of RlP(AEt RlP) for4 ***: Alterations in AD phenotypes were measured in Il-4/luc/CNS-1 Tg mice following treatment with AEt RlP using inflammation parameter analysis, bioluminescence imaging analysis, histological analysis, reverse transcription-polymerase chain reaction, enzyme linked immunosorbent assay and Western blot ***: RlP contained high concentrations of total phenolic compounds, total flavonoid compounds and 5-HNE related to AD therapy. The luc signal was only detected in the abdominal region and the submandibular lymph node(Sl), mesenteric lymph node(Ml), thymus and pancreas of the PA treated group. This signal was significantly decreased by 28%-73% throughout the body and in the four organs in PA + AEt RlP treated group. Furthermore, the lymph node weight, immunoglobulin E concentration and dermal thickness were decreased by 37%-67% in the PA + AEt RlP treated ***: Our findings suggest that the therapeutic effect of AEt RlP on PA induced AD could be successfully quantified by comparison of luc signals and AD phenotype markers in Il-4/luc/CNS-1Tg mice, and that the luc signal was as sensitive as the general AD phenotypes, enabling detection of effects without euthanasia.

关键词： Dermatitis, Atopic liriope plant Interleukin-4 luciferases Mice, transgenic Phthalic anhydrides Immunoglobulin E

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Knowledge, Attitudes and Practice Regarding Breast Cancer among Parakou University Female Students in 2021

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Open Journal of Pathology 2023年第1期13卷 28-39页

作者： luc Valère Codjo Brun luc Béhanzin Nukunté David lionel Togbenon Marie-Claire Assomption Oloufoudi Balle Pognon Yessito Corine Nadège Houéhanou-Sonou Sèdjro Raoul Atadé Christelle Doussengue Falilath Séidou Kabibou Salifou Marie Thérèse Akélé Akpo Faculty of Medicine University of Parakou Parakou Benin National School of Public Health and Epidemiology University of Parakou Parakou Benin Institute of Nursing and Obstetrical Care (IFSIO) University of Parakou Parakou Benin Faculty of Health Sciences University of Abomey Calavi Cotonou Benin

Background: Breast cancer is a life-threatening pathology affecting women across the world. Evidence suggests deficiency of knowledge and numerous sociodemographic factors contribute to delayed medical attention seeking behavior whether initial symptoms of breast cancer go undetected. Objective: This study aimed to assess knowledge, attitudes and practices regarding breast cancer among Parakou University female students in 2021. Method: This was a cross-sectional study with descriptive and analytical purposes, carried out on the campus of the University of Parakou, from June 14 to July 2, 2021. Data collection was performed through an individual online questionnaire, designed with KoBoCollect version 1.29.3. Data analysis was implemented with EPI INFO VERSION 7.***.3.3 software. Result: A total of 200 students meeting the inclusion criteria participated in this study. Among them, 20.5% had never heard about breast cancer. Non practice of breast cancer screening (breast self-examination) prevalence was 80%. Factors favoring non practice of breast cancer screening were: ethnicity, religion, not adhering to screening ideas, not committing to educating family and friends about screening. Factors promoting breast cancer screening practice were: having ever heard about breast cancer, being able to point to breast cancer symptoms, having adequate knowledge of breast cancer screening methods, having a good knowledge of the late-stage screening drawbacks. Conclusion: Some young women with high school level are still unaware of breast cancer and do not practice screening. Policymakers should consider more effective information outreach.

关键词： Knowledge Attitudes Practice Breast Cancer Parakou (Benin)

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海南高种椰子全基因组测序与分析

海南高种椰子全基因组测序与分析

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2017年全国热带作物学术年会

作者：杨耀东肖勇许鹏威 luc Baudouin 范海阔吴翼李静彭明中国热带农业科学院椰子研究所华大基因法国农业国际合作研究发展中心中国热带农业科学院热带生物技术研究所

椰子（Cocos nucifera l）是热带地区重要的水果和油料经济作物,栽培历史悠久、用途广泛、经济价值巨大,但是由于椰子的种果少,繁育周期长,传统的育种方法不能满足当前的椰子产业发展的需求,快速发展的分子标记技术已经在其它作物的种...

椰子（Cocos nucifera l）是热带地区重要的水果和油料经济作物,栽培历史悠久、用途广泛、经济价值巨大,但是由于椰子的种果少,繁育周期长,传统的育种方法不能满足当前的椰子产业发展的需求,快速发展的分子标记技术已经在其它作物的种质资源评价和育种研究中得到了广泛的应用,但是由于椰子基因组信息的缺失限制了椰子分子标记的开发、性状的关联及其在育种中的应用等工作的开展。本研究以海南高种椰子为对象开展椰子全基因组测序与分析。通过9个DNA（170bp-40kb）插入文库的测序和数据过滤后共获得419.08 Gb的干净reads,K-mer分析预测了椰子的基因组大小为2.42Gb.用SOAPdenovo2软件进行contig和scaffold的构建及gap filling,最后获得长度为2.20Gb的椰子基因组草图（相当于90.91%的预测基因组的大小）。通过同源性比对,重头预测和转录本比对等方法我们共预测了28,039蛋白质编码基因,其中21,087蛋白序列具有保守域,15,705个被注释到1,622 Gene Ontology （go）功能类别,分析发现椰子的这些基因归属于14,411基因家族,其中有282基因家族是椰子特有的。进化分析显示椰子和油棕的分歧时间大约在46百万年前晚于椰枣的分歧时间。通过椰子作图群体的GBS分析,共发现621,283SNP标记,其中8,402个SNP被分配到2,303 scaffolds上,通过这些标记,我们成功地构建了椰子的16条染色体并对染色体的SNP标记的分布、基因密度、GC含量、共线性等特性进行了分析,通过比较基因组分析,我们重构了椰子、油棕和椰枣的9条古生染色体,在这些物种的演化过程中,出现了一次的染色体加倍变成了18条,通过后续的两次的融合和两次的分裂变成了现在的椰子和油棕的16条染色体。我们还结合椰子盐胁迫下的表达谱数据开展了椰子喜盐特性的组学分析,为探索椰子对高盐环境适应的分子机制提供了基础。本研究为椰子功能基因组的研究提供了一个参考基因组,该研究将为利于主要棕榈作物比较基因组工作的开展,为椰子DNA分子标记的开发及椰子重要农艺性状的解析及关联分析提供基础,为椰子的全基因组关联分析提供参考体系,促进椰子分子辅助育种工作的开展。

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