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luminescence of coelenterazine derivatives with C-8 extended electronic conjugation

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Chinese Chemical Letters 2016年第4期27卷 550-554页

作者： Ming-Liang Yuan Tian-Yu Jiang lu-pei du Min-Yong Li Department of Medicinal Chemistry Key Laboratory of Chemical Biology (MOE) School of Pharmacy Shandong University

Replacement of the methylene group at the C-8 position with an extended electronic conjugation is a new promising method to develop red-shifted coelenterazine derivatives. In this paper, we have described an oxygen-containing coelenterazine derivative with a significant red-shifted（63 nm）bioluminescence signal maximum relative to coelenterazine 400a（Deep Blue C^（TM）, 1）. In cell imaging, the sulfur-containing coelenterazine derivative displayed a significantly（1.77 ± 0.09;P≤0.01） higher luminescence signal compared to coelenterazine 400 a and the oxygen-containing coelenterazine derivative exhibited a slightly（0.74 ± 0.08; P≤0.05） lower luminescence signal. It is beneficial to understand further the underlying mechanisms of bioluminescence.

关键词： Bioluminescence imaging Coelenterazine derivatives Relative quantum yield Chemiluminescence Red-shifted

Discovery of naphthalimide conjugates as fluorescent probes for α_1-adrenoceptors

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Chinese Chemical Letters 2016年第2期27卷 185-189页

作者： Wei Zhang Xin-Yang Zhou Qin-Ying Yu lu-pei du Min-Yong Li Department of Medicinal Chemistry Key Laboratory of Chemical Biology (MOE) School of Pharmacy Shandong University

α1-Adrenoceptors（α1-ARs）, including at least three subtypes, α（1A）, α（1B） and α（1D）, which play essential roles in G protein-coupled receptors（GPCRs）, can convey multiple pivotal extracellular signals in varied tissues and organs. In this research, a series of napthalimide-based small-molecule fluorescent probes（1a-1f） for α1-ARs, including two parts, a pharmacophore（quinazoline and phenylpiperazine） for α1-AR recognition and a fluorophore（naphthalimide） for visualization, were designed and synthesized successfully. These compounds display excellent fluorescence property and high affinity to receptors,which were used successfully for in vitro visualization of α1-adrenoceptors.

关键词： α1-Adrenergic receptors Fluorescent probes High affinity Cell imaging

A bestatin-based fluorescent probe for aminopeptidase N cell imaging

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Chinese Chemical Letters 2015年第5期26卷 513-516页

作者： Hui Chen Hui Wang Xiao-Jun Qin Chen Chen lu Feng Lai-Zhong Chen lu-pei du Min-Yong Li Department of Medicinal Chemistry Key Laboratory of Chemical Biology(MOE)School of PharmacyShandong University China Pharmaceutical University Pharmaceutical Co.Ltd.

Aminopeptidase N （APN） is an important drug target and biomarker for various tumors. The current work characterizes a novel APN-targeted fluorescent probe （Bes-Green, 2） that manifests comparable inhibitory activity with Bestatin. This probe has capacity of tightly binding to the APN for imaging endogenous APN in living human ovarian clear cell carcinoma cells （ES-2） and has potential application in biological study of cellular APN.

关键词： Aminopeptidase N Bestatin Cell imaging Fluorescent probe

Diagnostic Techniques for COVID-19:A Mini-review of Early Diagnostic Methods

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Journal of Analysis and Testing 2021年第4期5卷 314-326页

作者： Gao-Pan Dong Xiu-Juan Guo Ying-Ai Sun Zheng Zhang lu-pei du Min-Yong Li Department of Medicinal Chemistry Key Laboratory of Chemical Biology(MOE)School of Pharmaceutical SciencesCheeloo College of MedicineShandong UniversityJinan 250012China School of Pharmacy Xinxiang Medical UniversityXinxiang 453003HenanChina

The outbreak of severe pneumonia at the end of 2019 was proved to be caused by the SARS-CoV-2 virus spreading out the *** COVID-19 spread rapidly through a terrible transmission way by human-to-human,which led to many suspected cases waiting to be diagnosed and huge daily samples needed to be tested by an eff ective and rapid detection *** an increasing number of COVID-19 infections,medical pressure is ***,more e fficient and accurate diagnosis methods were keen urgently *** this review,we summarized several methods that can rapidly and sensitively identify COVID-19;some of them are widely used as the diagnostic techniques for SARS-CoV-2 in various countries,some diagnostic technologies refer to SARS(Severe Acute Respiratory Syndrome)or/and MERS(Middle East Respiratory Syndrome)detection,which may provide potential diagnosis ideas.

关键词： COVID-19 Severe respiratory disease SARS-CoV-2 virus Rapid and accurate methods Diagnostic approach

Peptidase Activities of Tripeptidyl Peptidase Ⅰ (TPP Ⅰ): Exopeptidase and Endopeptidase

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Chemical Research in Chinese Universities 2006年第1期22卷 65-67页

作者： du pei-ge AN Li-ping QIU Fang-ping lu Gang Clinical Diagnosis Laboratory of Beihua University Jilin 132013 P. R. China College of Biological Engineering Changchun University of Technology Changchun 130012 P. R. China

The defect of TPP Ⅰ causes a disease, late infantile neuronal ceroid lipofuscinosis（LINCL, CLN2）. To investigate the bio-activity of tripeptidyl peptidase Ⅰ （TPP Ⅰ ） from rat kidneys, the effects of digestion of angiotensin Ⅱ （Ang Ⅱ ） and a synthetic endo-type substrate（ Gly^1-Lys-Pro^5-lie-Pro^5-Phe-Phe-Arg-Leu-Lys^10） via TPP I were ana- lyzed by HPLC and TOF-MS. The data suggest that the degradation rate of Ang I1 can reach 18. 2% by the rat TPP I and DRV（Asp-Arg-Val） can be released from N-termini of Ang Ⅱ within 16 h. In addition, the synthetic endotype substrate is cleaved at the same position between Phe6 and Phe^7. Accordingly, TPP Ⅰ shows two kinds of peptidase activities. One is a tripeptidyl peptidase activity and the other is a pepstatin insensitive carboxyl endopeptidase activity. Tripeptidyl peptidase activity and pepstatin insensitive carboxyl endopeptidase activity seem to be dual phases of one enzyme, TPP Ⅰ.

关键词： Rat TPP Ⅰ Angiotensin Ⅱ Endo-type substrate Endopeptidase activity

Nonlinear dynamics of a classical rotating pendulum system with multiple excitations

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Chinese Physics B 2020年第11期29卷 230-243页

作者： Ning Han pei-pei lu Key Laboratory of Machine Learning and Computational Intelligence College of Mathematics and Information ScienceHebei UniversityBaoding 071002China

We report an attempt to reveal the nonlinear dynamic behavior of a classical rotating pendulum system subjected to combined excitations of constant force and periodic *** unperturbed system characterized by strong irrational nonlinearity bears significant similarities to the coupling of a simple pendulum and a smooth and discontinuous(SD)oscillator,especially the phase trajectory with coexistence of duffing-type and pendulum-type homoclinic *** order to learn the effect of constant force on this pendulum system,all types of phase portraits are displayed by means of the Hamiltonian function with large constant excitation especially the transitions of complex singular closed *** sufficiently small perturbations of the viscous damping and constant excitation,the Melnikov method is used to analyze the global structure of the phase space and the feature of *** is shown,both theoretically and numerically,that this system undergoes a homoclinic bifurcation and then bifurcates a unique attracting rotating limit ***,the estimation of the chaotic threshold of the rotating pendulum system with multiple excitations is calculated and the predicted periodic and chaotic motions can be shown by applying numerical simulations.

关键词： rotating pendulum Melnikov method rotating limit cycle chaotic dynamics

Exploring charge and spin fluctuations in infinite-layer cuprate SrCuO_(2)from a phonon perspective

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Science China(Physics,Mechanics & Astronomy) 2024年第8期67卷 159-167页

作者： Xin du pei-Han Sun Ben-Chao Gong Jian-Feng Zhang Zhong-Yi lu Kai Liu Department of Physics and Beijing Key Laboratory of Opto-electronic Functional Materials&Micro-nano Devices Renmin University of ChinaBeijing 100872China Department of Physics School of ScienceJiangsu University of Science and TechnologyZhenjiang 212100China Institute of Physics Chinese Academy of SciencesBeijing 100190China Key Laboratory of Quantum State Construction and Manipulation(Ministry of Education) Renmin University of ChinaBeijing 100872China

The infinite-layer cuprate ACu O_(2)(A=Ca,Sr,Ba)possesses the simplest crystal structure among numerous cuprate superconductors and can serve as a prototypical system to explore the unconventional *** on the first-principles electronic structure calculations,we have studied the electronic and magnetic properties of the infinite-layer cuprate SrCuO_(2)from a phonon *** find that interesting fluctuations of charges,electrical dipoles,and local magnetic moments can be induced by the atomic displacements of phonon modes in SrCuO_(2)upon the hole *** all optical phonon modes of SrCuO_(2)in the antiferromagnetic Néel state,only the A_(1g)mode that involves the full-breathing O vibrations along the Cu-O bonds can cause significant fluctuations of local magnetic moments on O atoms and dramatic charge redistributions between Cu and O ***,due to the atomic displacements of the A_(1g)mode,both the charge fluctuations on Cu and the electrical dipoles on O show a dome-like evolution with increasing hole doping,quite similar to the experimentally observed behavior of the superconducting T_(c);in comparison,the fluctuations of local magnetic moments on O display a monotonic enhancement along with the hole *** analyses indicate that around the optimal doping,there exists a large softening in the frequency of the A_(1g)phonon mode and a van Hove singularity in the electronic structure close to the Fermi level,suggesting potential electron-phonon *** work reveals the important role of the full-breathing O phonon mode playing in the infinite-layer SrCuO_(2),which may provide new insights in understanding the cuprate superconductivity.

关键词： first-principles calculations cuprate superconductors phonon charge and spin fluctuations

IDENTIFYING INFluENTIAL SPREADERS IN ARTIFICIAL COMPLEX NETWORKS

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Journal of Systems Science & Complexity 2014年第4期27卷 650-665页

作者： WANG pei TIAN Chengeng lu Jun-an School of Mathematics and Information Sciences Henan University School of Mathematics and Statistics Wuhan University

A long-term common belief in complex networks is that,the most connected nodes are the most efficient ***,recent investigations on real-world complex networks show that the most influential spreaders are those with the highest fc-shell *** is well-known that,many real-world complex networks have scale free(SF),small world(SW) properties,therefore,identification of influential spreaders in general artificial SF,SW as well as random networks will be more *** research finds that,for artificial ER and SW networks,degree is more reliable than fc-shell in predicting the outcome of ***,for artificial SF networks,fc-shell is remarkably reliable than degree and betweeness,which indicate that the four recently investigated real-world networks[Kitsak M,Gallos L K,Havlin S,Liljeros F,Muchnik L,Stanley H E,Makse H A,Identification of influential spreaders in complex networks,***.,2010,6:888-893.]are more similar to scale free ***,the investigations also indicate us an optimal dissemination strategy in networks with scale free *** is,starting from moderate-degree-nodes will be ok and even more economical,since one can derive roughly similar outcome with starting from hubs.

关键词： Complex network influential spreader k-shell scale free small world.

CFD simulation of bubbling and collapsing characteristics in a gas-solid fluidized bed

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Petroleum Science 2009年第1期6卷 69-75页

作者： pei pei Zhang Kai lu Erwei Wen Dongsheng State Key Laboratory of Heavy Oil Processing China University of Petroleum Beijing 102249 China School of Engineering and Materials Science Queen Mary University of London Mile End Road London UK E1 4NS

Computational Fluid Dynamics （CFD） has become an alternative method to experiments for understanding the fluid dynamics of multiphase flow. A two-fluid model, which contains additional terms in both the gas- and solid-phase momentum equations, is used to investigate the fluidization quality in a fluidized bed. A case study for quartz sand with a density of 2,660 kg/m^3 and a diameter of 500 μm, whose physical property is similar to a new kind of catalyst for producing clean fuels through the residue fluid catalytic cracking process, is simulated in a two-dimensional fluidized bed with 0.57 m width and 1.00 m height. Transient bubbling and collapsing characteristics are numerically investigated in the platform of CFX 4.4 by integrating user-defined Fortran subroutines. The results show that the fluidization and collapse process is in fair agreement with the classical theory of Geldart B classification, but the collapse time is affected by bubbles at the interface between the dense phase and freeboard.

关键词： Fluidized bed CFD simulation bubbling and collapsing behaviors